United States
Germany
Japan
United Kingdom
China
USH2A (NM_007123) Human Tagged ORF Clone
CAT＃: RG221475
- TrueORF®
USH2A (tGFP-tagged) - Human Usher syndrome 2A (autosomal recessive, mild) (USH2A), transcript variant 1
ORF Plasmid: DDK
"NM_007123" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 16,910.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | dJ1111A8.1; RP39; US2; USH2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221475 representing NM_007123
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTGCCCAGTTCTTTCATTGGGCTCTGGCTTCTTGTTTCAGGTCATTGAAATGTTGATCTTTGCCT ATTTTGCTTCAATATCCTTGACTGAGTCACGAGGTCTTTTCCCAAGGCTGGAGAACGTGGGAGCTTTCAA GAAAGTTTCCATCGTGCCAACCCAAGCAGTATGTGGACTCCCAGACCGAAGCACTTTTTGTCACAGCTCT GCTGCTGCTGAAAGTATTCAGTTCTGTACCCAGCGGTTTTGTATTCAGGATTGCCCATACAGATCTTCAC ACCCTACCTACACTGCCCTTTTCTCAGCAGGCCTCAGTAGCTGCATCACACCAGACAAGAATGATCTGCA TCCTAACGCCCATAGCAATTCTGCAAGTTTTATTTTTGGAAATCACAAGAGCTGCTTTTCTTCTCCTCCT TCTCCAAAGCTGATGGCATCATTTACCTTAGCTGTATGGCTGAAACCTGAGCAACAAGGTGTAATGTGTG TTATAGAAAAGACAGTAGATGGGCAGATTGTGTTCAAACTTACAATATCTGAGAAAGAGACCATGTTTTA TTATCGCACAGTAAATGGTTTGCAACCTCCAATAAAAGTAATGACACTGGGGAGAATTCTTGTGAAGAAA TGGATTCATCTTAGTGTGCAGGTGCATCAGACAAAAATCAGCTTCTTTATCAATGGCGTGGAGAAGGATC ATACACCTTTCAATGCAAGAACTCTAAGTGGTTCAATTACAGATTTTGCATCTGGTACTGTGCAAATAGG ACAGAGTTTAAATGGTTTAGAGCAGTTTGTCGGAAGAATGCAAGATTTTCGATTATACCAAGTGGCACTT ACAAACAGAGAGATTCTGGAAGTCTTCTCTGGAGATCTTCTCAGATTGCATGCCCAATCACATTGCCGTT GCCCTGGCAGCCACCCGCGGGTCCACCCTTTGGCACAGCGGTACTGCATTCCTAATGATGCAGGAGACAC AGCTGATAATAGAGTGTCACGGTTGAATCCTGAAGCCCATCCTCTCTCTTTTGTCAATGATAATGATGTT GGTACTTCATGGGTTTCAAATGTGTTTACAAACATTACACAGCTTAATCAAGGAGTGACTATTTCAGTTG ATTTGGAAAATGGACAGTATCAGGTGTTTTATATTATCATTCAGTTCTTTAGTCCACAACCAACGGAAAT AAGGATTCAAAGGAAGAAGGAAAATAGTTTAGATTGGGAGGACTGGCAATATTTTGCCAGGAATTGTGGT GCTTTTGGAATGAAAAACAATGGAGATTTGGAAAAACCTGATTCTGTCAACTGTCTTCAGCTTTCCAATT TTACTCCATATTCCCGTGGCAATGTCACATTTAGCATCCTGACACCTGGACCAAATTATCGTCCTGGATA CAATAACTTCTATAATACCCCATCTCTTCAAGAGTTCGTAAAAGCCACGCAAATAAGGTTTCATTTTCAT GGGCAGTACTATACAACTGAGACTGCTGTTAACCTCAGACACAGATATTATGCAGTGGACGAAATCACCA TTAGTGGGAGATGTCAGTGCCATGGTCATGCCGATAACTGCGACACAACAAGCCAGCCATATAGATGCCT CTGCTCCCAGGAGAGCTTCACTGAAGGACTTCATTGTGATCGCTGCTTGCCTCTTTATAATGACAAGCCT TTCCGCCAAGGTGATCAAGTTTACGCTTTCAATTGTAAACCTTGTCAATGCAACAGCCATTCCAAAAGCT GCCATTACAACATCTCTGTAGACCCATTTCCTTTTGAGCACTTCAGAGGGGGAGGAGGAGTTTGTGATGA TTGTGAGCATAACACTACAGGAAGGAACTGTGAGCTGTGCAAGGATTACTTTTTCCGACAAGTTGGTGCA GATCCTTCGGCCATAGATGTTTGCAAACCCTGTGACTGTGATACAGTTGGCACTAGAAATGGTAGCATTC TTTGTGATCAGATTGGAGGACAGTGTAATTGTAAGAGACACGTGTCTGGCAGGCAGTGCAATCAGTGCCA GAATGGATTCTACAATCTACAAGAGTTGGATCCTGATGGCTGCAGTCCCTGTAACTGCAATACCTCTGGG ACAGTGGATGGAGATATTACCTGTCACCAAAATTCAGGCCAGTGCAAGTGCAAAGCAAACGTTATTGGGC TTAGGTGTGATCATTGCAATTTTGGATTTAAATTTCTCCGAAGCTTTAATGATGTTGGATGTGAGCCCTG CCAGTGTAACCTCCATGGCTCAGTGAACAAATTCTGCAATCCTCACTCTGGGCAGTGTGAGTGCAAAAAA GAAGCCAAAGGACTTCAGTGTGACACCTGCAGAGAAAACTTTTATGGGTTAGATGTCACCAATTGTAAGG CCTGTGACTGTGACACAGCTGGATCCCTCCCTGGGACTGTCTGTAATGCTAAGACAGGGCAGTGCATCTG CAAGCCCAATGTTGAAGGGAGACAGTGCAATAAATGTTTGGAGGGAAACTTCTACCTACGGCAAAATAAT TCTTTCCTCTGTCTGCCTTGCAACTGTGATAAGACTGGGACAATAAATGGCTCTCTGCTGTGTAACAAAT CAACAGGACAATGTCCTTGCAAATTAGGGGTAACAGGTCTTCGCTGTAATCAGTGTGAGCCTCACAGGTA CAATTTGACCATTGACAATTTTCAACACTGCCAGATGTGTGAGTGTGATTCCTTGGGGACATTACCTGGG ACCATTTGTGACCCAATCAGTGGCCAGTGCCTGTGTGTGCCTAATCGTCAAGGAAGAAGGTGTAATCAGT GTCAACCAGGTTTTTATATTTCTCCAGGCAATGCCACTGGCTGCCTGCCATGCTCATGCCATACAACTGG TGCAGTTAATCACATCTGTAATAGCCTGACTGGTCAGTGTGTTTGCCAAGATGCTTCCATTGCTGGGCAA CGTTGTGACCAATGCAAAGACCATTACTTTGGATTTGATCCTCAGACTGGAAGATGTCAGCCTTGTAATT GTCATCTCTCAGGAGCCTTGAATGAAACCTGTCACTTGGTCACAGGCCAGTGTTTCTGTAAACAATTTGT CACTGGCTCAAAGTGTGATGCTTGTGTTCCCAGTGCAAGCCACTTGGATGTCAACAATCTATTGGGTTGC AGCAAAACTCCATTCCAGCAACCTCCGCCCAGAGGACAAGTTCAAAGTTCTTCTGCTATCAATCTCTCCT GGAGTCCACCTGATTCTCCAAATGCCCACTGGCTTACTTACAGTTTACTCAGGGATGGTTTTGAAATCTA CACAACAGAGGATCAATACCCATACAGTATTCAATACTTCTTAGACACAGACCTGTTACCATATACCAAA TATTCCTATTACATTGAGACCACCAATGTGCATGGTTCAACAAGGAGTGTAGCTGTCACTTACAAGACAA AACCAGGGGTCCCAGAGGGAAACTTGACTTTAAGTTATATCATTCCTATTGGCTCAGACTCTGTGACACT TACCTGGACAACACTCTCAAATCAATCTGGTCCCATAGAGAAATATATTTTGTCCTGTGCCCCTTTGGCT GGTGGTCAGCCATGTGTTTCCTACGAAGGTCATGAAACCTCAGCTACCATCTGGAATCTGGTTCCATTTG CCAAGTACGATTTTTCTGTACAGGCGTGTACTAGCGGGGGCTGTTTACACAGCTTGCCCATTACAGTGAC CACAGCCCAGGCCCCTCCCCAAAGACTAAGTCCACCTAAGATGCAGAAAATCAGTTCTACAGAACTTCAT GTAGAATGGTCTCCACCAGCGGAACTAAATGGAATAATTATAAGATATGAACTATACATGAGAAGACTGA GATCTACTAAAGAAACCACATCTGAGGAAAGTCGAGTTTTTCAGAGCAGTGGTTGGCTCAGTCCTCATTC ATTTGTAGAATCGGCCAATGAAAATGCATTAAAACCTCCTCAAACAATGACAACCATCACTGGCTTGGAG CCATACACCAAGTATGAGTTCAGAGTCTTAGCTGTGAATATGGCTGGAAGTGTGTCTTCTGCCTGGGTCT CAGAAAGAACGGGAGAATCAGCACCTGTATTCATGATCCCTCCTTCAGTCTTTCCCCTCTCTTCGTACTC TCTCAATATCTCCTGGGAGAAGCCAGCAGATAATGTTACAAGAGGAAAAGTTGTGGGGTATGACATCAAT ATGCTTTCTGAACAATCACCTCAACAGTCTATTCCCATGGCGTTTTCACAGCTGTTGCACACTGCTAAAT CCCAAGAACTATCTTACACTGTAGAAGGACTGAAACCTTATAGGATATATGAGTTTACTATTACTCTCTG CAATTCAGTTGGTTGTGTGACCAGTGCTTCGGGAGCAGGACAAACTTTAGCAGCAGCACCAGCACAACTG AGGCCACCTCTGGTTAAAGGAATCAACAGCACAACAATCCATCTTAAGTGGTTTCCACCTGAAGAACTGA ATGGACCCTCTCCTATATATCAGCTGGAAAGGAGAGAGTCATCTCTACCAGCTCTGATGACCACGATGAT GAAAGGAATCCGTTTCATAGGAAATGGGTATTGTAAATTTCCCAGCTCCACTCACCCAGTCAATACAGAC TTCACTGGTAAGTGTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221475 representing NM_007123
Red=Cloning site Green=Tags(s) MNCPVLSLGSGFLFQVIEMLIFAYFASISLTESRGLFPRLENVGAFKKVSIVPTQAVCGLPDRSTFCHSS AAAESIQFCTQRFCIQDCPYRSSHPTYTALFSAGLSSCITPDKNDLHPNAHSNSASFIFGNHKSCFSSPP SPKLMASFTLAVWLKPEQQGVMCVIEKTVDGQIVFKLTISEKETMFYYRTVNGLQPPIKVMTLGRILVKK WIHLSVQVHQTKISFFINGVEKDHTPFNARTLSGSITDFASGTVQIGQSLNGLEQFVGRMQDFRLYQVAL TNREILEVFSGDLLRLHAQSHCRCPGSHPRVHPLAQRYCIPNDAGDTADNRVSRLNPEAHPLSFVNDNDV GTSWVSNVFTNITQLNQGVTISVDLENGQYQVFYIIIQFFSPQPTEIRIQRKKENSLDWEDWQYFARNCG AFGMKNNGDLEKPDSVNCLQLSNFTPYSRGNVTFSILTPGPNYRPGYNNFYNTPSLQEFVKATQIRFHFH GQYYTTETAVNLRHRYYAVDEITISGRCQCHGHADNCDTTSQPYRCLCSQESFTEGLHCDRCLPLYNDKP FRQGDQVYAFNCKPCQCNSHSKSCHYNISVDPFPFEHFRGGGGVCDDCEHNTTGRNCELCKDYFFRQVGA DPSAIDVCKPCDCDTVGTRNGSILCDQIGGQCNCKRHVSGRQCNQCQNGFYNLQELDPDGCSPCNCNTSG TVDGDITCHQNSGQCKCKANVIGLRCDHCNFGFKFLRSFNDVGCEPCQCNLHGSVNKFCNPHSGQCECKK EAKGLQCDTCRENFYGLDVTNCKACDCDTAGSLPGTVCNAKTGQCICKPNVEGRQCNKCLEGNFYLRQNN SFLCLPCNCDKTGTINGSLLCNKSTGQCPCKLGVTGLRCNQCEPHRYNLTIDNFQHCQMCECDSLGTLPG TICDPISGQCLCVPNRQGRRCNQCQPGFYISPGNATGCLPCSCHTTGAVNHICNSLTGQCVCQDASIAGQ RCDQCKDHYFGFDPQTGRCQPCNCHLSGALNETCHLVTGQCFCKQFVTGSKCDACVPSASHLDVNNLLGC SKTPFQQPPPRGQVQSSSAINLSWSPPDSPNAHWLTYSLLRDGFEIYTTEDQYPYSIQYFLDTDLLPYTK YSYYIETTNVHGSTRSVAVTYKTKPGVPEGNLTLSYIIPIGSDSVTLTWTTLSNQSGPIEKYILSCAPLA GGQPCVSYEGHETSATIWNLVPFAKYDFSVQACTSGGCLHSLPITVTTAQAPPQRLSPPKMQKISSTELH VEWSPPAELNGIIIRYELYMRRLRSTKETTSEESRVFQSSGWLSPHSFVESANENALKPPQTMTTITGLE PYTKYEFRVLAVNMAGSVSSAWVSERTGESAPVFMIPPSVFPLSSYSLNISWEKPADNVTRGKVVGYDIN MLSEQSPQQSIPMAFSQLLHTAKSQELSYTVEGLKPYRIYEFTITLCNSVGCVTSASGAGQTLAAAPAQL RPPLVKGINSTTIHLKWFPPEELNGPSPIYQLERRESSLPALMTTMMKGIRFIGNGYCKFPSSTHPVNTD FTGKCV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007123 |
| ORF Size | 4638 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007123.5, NP_009054.5 |
| RefSeq Size | 6332 bp |
| RefSeq ORF | 4641 bp |
| Locus ID | 7399 |
| UniProt ID | O75445 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a protein that contains laminin EGF motifs, a pentaxin domain, and many fibronectin type III motifs. The protein is found in the basement membrane, and may be important in development and homeostasis of the inner ear and retina. Mutations within this gene have been associated with Usher syndrome type IIa and retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221475 | USH2A (Myc-DDK-tagged)-Human Usher syndrome 2A (autosomal recessive, mild) (USH2A), transcript variant 1 |
CNY 15,390.00 |
|
| RC221475L3 | Lenti-ORF clone of USH2A (Myc-DDK-tagged)-Human Usher syndrome 2A (autosomal recessive, mild) (USH2A), transcript variant 1 |
CNY 17,290.00 |
|
| RC221475L4 | Lenti-ORF clone of USH2A (mGFP-tagged)-Human Usher syndrome 2A (autosomal recessive, mild) (USH2A), transcript variant 1 |
CNY 17,290.00 |
|
| SC303881 | USH2A (untagged)-Human Usher syndrome 2A (autosomal recessive, mild) (USH2A), transcript variant 1 |
CNY 29,640.00 |
