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C15ORF27 (TMEM266) (NM_152335) Human Tagged ORF Clone
CAT＃: RG221441
- TrueORF®
C15orf27 (tGFP-tagged) - Human chromosome 15 open reading frame 27 (C15orf27)
ORF Plasmid: DDK
"NM_152335" in other vectors (5)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C15orf27; HsHVRP1; hTMEM266; HVRP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221441 representing NM_152335
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGCTCCATCTTTCAACATGACCAATCCACAGCCTGCCATAGAAGGAGGAATTTCTGAAGTTG AGATCATCTCCCAACAAGTAGACGAAGAAACCAAGAGCATTGCTCCTGTGCAGCTGGTGAACTTTGCCTA TCGGGACTTGCCCCTGGCTGCTGTCGATCTCTCCACGGCGGGCTCGCAGCTCCTGTCAAATCTGGACGAA GATTACCAAAGAGAAGGGTCTAACTGGCTGAAGCCGTGCTGTGGGAAGAGAGCAGCCGTGTGGCAGGTAT TTTTGCTCAGTGCAAGTCTCAACAGTTTCCTGGTAGCCTGTGTAATATTGGTGGTGATTCTCCTGACTCT GGAACTTCTAATAGATATAAAGCTTCTCCAGTTTTCCAGCGCATTCCAGTTTGCTGGCGTGATTCACTGG ATCAGCCTGGTCATTCTGTCCGTGTTCTTCTCAGAGACTGTTCTACGGATTGTGGTGCTTGGGATCTGGG ATTACATCGAAAACAAAATAGAGGTGTTTGACGGGGCTGTGATCATCCTATCTTTGGCTCCGATGGTGGC ATCCACTGTGGCCAATGGACCCAGGAGCCCCTGGGACGCCATCAGCCTCATCATCATGCTCCGGATCTGG AGGGTGAAGAGGGTCATTGATGCCTACGTCCTGCCAGTGAAGCTGGAGATGGAGATGGTTATCCAGCAGT ACGAGAAGGCCAAGGTCATCCAAGACGAGCAGCTGGAGAGGCTGACGCAGATCTGTCAGGAGCAAGGGTT TGAGATCCGGCAGCTGCGCGCGCACCTGGCGCAGCAGGACCTGGACCTGGCTGCCGAGCGCGAAGCGGCG CTCCAGGCCCCGCACGTGCTCAGCCAGCCGCGCAGCCGCTTCAAAGTGTTGGAGGCCGGCACGTGGGACG AGGAGACGGCGGCCGAGAGCGTCGTGGAGGAGCTGCAGCCCTCGCAAGAAGCCACGATGAAGGACGACAT GAACAGCTACATCAGTCAGTATTACAATGGGCCCAGCAGTGACAGCGGTGTCCCAGAGCCAGCTGTGTGT ATGGTCACCACGGCCGCAATAGACATTCACCAGCCCAACATCTCCTCGGACCTCTTCTCTCTGGACATGC CCCTCAAACTCGGCGGTAATGGCACCAGCGCCACCTCGGAGAGTGCCTCCCGCAGCTCAGTCACCCGGGC CCAGAGTGACAGCAGCCAGACGCTGGGCTCCTCCATGGACTGCAGCACTGCCCGCGAGGAGCCGTCCTCT GAGCCCGGCCCTTCTCCCCCGCCGCTGCCATCCCAGCAGCAGGTGGAGGAGGCCACAGTCCAGGACCTGC TGTCCTCCCTGTCGGAGGACCCCTGCCCTTCCCAGAAGGCCTTGGACCCAGCCCCCCTCGCCCGGCCCAG CCCAGCGGGCTCGGCCCAAACCAGCCCCGAGCTGGAACACAGGGTAAGTCTGTTCAACCAGAAGAACCAG GAGGGCTTCACTGTCTTTCAGATCAGGCCTGTCATCCACTTCCAGCCCACTGTGCCCATGCTGGAGGACA AGTTCAGATCTTTGGAATCCAAAGAGCAAAAGCTGCACAGGGTCCCTGAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221441 representing NM_152335
Red=Cloning site Green=Tags(s) MAVAPSFNMTNPQPAIEGGISEVEIISQQVDEETKSIAPVQLVNFAYRDLPLAAVDLSTAGSQLLSNLDE DYQREGSNWLKPCCGKRAAVWQVFLLSASLNSFLVACVILVVILLTLELLIDIKLLQFSSAFQFAGVIHW ISLVILSVFFSETVLRIVVLGIWDYIENKIEVFDGAVIILSLAPMVASTVANGPRSPWDAISLIIMLRIW RVKRVIDAYVLPVKLEMEMVIQQYEKAKVIQDEQLERLTQICQEQGFEIRQLRAHLAQQDLDLAAEREAA LQAPHVLSQPRSRFKVLEAGTWDEETAAESVVEELQPSQEATMKDDMNSYISQYYNGPSSDSGVPEPAVC MVTTAAIDIHQPNISSDLFSLDMPLKLGGNGTSATSESASRSSVTRAQSDSSQTLGSSMDCSTAREEPSS EPGPSPPPLPSQQQVEEATVQDLLSSLSEDPCPSQKALDPAPLARPSPAGSAQTSPELEHRVSLFNQKNQ EGFTVFQIRPVIHFQPTVPMLEDKFRSLESKEQKLHRVPEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152335 |
| ORF Size | 1593 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152335.3 |
| RefSeq Size | 2414 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 123591 |
| UniProt ID | Q2M3C6 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | Voltage-sensor protein present on the post-synaptic side of glutamatergic mossy fibers and granule cells in the cerebellum (PubMed:25165868, PubMed:30810529). Despite the presence of a voltage-sensor segment, does not form a functional ion channel and its precise role remains unclear (PubMed:25165868, PubMed:30810529). Undergoes both rapid and slow structural rearrangements in response to changes in voltage (PubMed:30810529). Contains a zinc-binding site that can regulate the slow conformational transition (PubMed:30810529).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221441 | C15orf27 (Myc-DDK-tagged)-Human chromosome 15 open reading frame 27 (C15orf27) |
CNY 3,952.00 |
|
| RC221441L3 | Lenti ORF clone of Human chromosome 15 open reading frame 27 (C15orf27), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC221441L4 | Lenti ORF clone of Human chromosome 15 open reading frame 27 (C15orf27), mGFP tagged |
CNY 6,460.00 |
|
| SC101016 | C15orf27 (untagged)-Human chromosome 15 open reading frame 27 (C15orf27) |
CNY 3,656.00 |
|
| SC317901 | C15orf27 (untagged)-Human chromosome 15 open reading frame 27 (C15orf27) |
CNY 8,550.00 |
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