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MRTFA (NM_020831) Human Tagged ORF Clone
CAT＃: RG221212
- TrueORF®
MKL1 (tGFP-tagged) - Human megakaryoblastic leukemia (translocation) 1 (MKL1)
ORF Plasmid: DDK
"NM_020831" in other vectors (4)
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CNY 11,824.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BSAC; MAL; MKL; MKL1; MRTF-A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221212 representing NM_020831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCCAGGACAGAGG ACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTCAGGATGCACATTTTGGAAGA GACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGACTAGCCGATGACCTC AATGAGAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAGTCCA GCCTGAAGGAAGCCATCATTGTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTCGATGA GGACAGCAGCGATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCGTCACCC CTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGGTTGTGTCTCAAC TTCCGATGGGCCGGGATTCCAGAGAAATGCTTTTCCTGGCAGAGCAGCCTCCTCTGCCTCCCCCACCTCT GCTGCCTCCCAGCCTCACCAATGGAACCACTATCCCCACTGCCAAGTCCACCCCCACACTCATTAAGCAA AGCCAACCCAAGTCTGCCAGTGAGAAGTCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGA AGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCCCCCATGGACTC ATCCTACGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAACCAGCAGCAGCAG CAGCACCACAACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCCCTGGGAAGCAGCG GGACCCCCCCAGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCGGGCGCCCCTGGGCCCTGTGG GCTGGCACGTCAGAACAGCACCTCACTGACTGGCAAGCCGGGAGCCCTGCCGGCCAACCTGGACGACATG AAGGTGGCAGAGCTGAAGCAGGAGCTGAAGTTGCGATCACTGCCTGTCTCGGGCACCAAAACTGAGCTGA TTGAGCGCCTTCGAGCCTATCAAGACCAAATCAGCCCTGTGCCAGGAGCCCCCAAGGCCCCTGCCGCCAC CTCTATCCTGCACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGGCTGAGCACGGGGCCAGCC CTGGTGGCAGCAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCCAGCAGTGGGGTGGTGAAGT TTGGCAGCACGGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGCTCACTGCTCAGCACGGGCGA TGAAAACTCCACCCCCGGGGACACCTTTGGTGAGATGGTGACATCACCTCTGACGCAGCTGACCCTGCAG GCCTCGCCACTGCAGATCCTCGTGAAGGAGGAGGGCCCCCGGGCCGGGTCCTGTTGCCTGAGCCCTGGGG GGCGGGCGGAGCTAGAGGGGCGCGACAAGGACCAGATGCTGCAGGAGAAAGACAAGCAGATCGAGGCGCT GACGCGCATGCTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAGCTGCAGCTGGAGCAGGAGAAGCGA GCCCAGCAGCCCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTGAAGCAGGAGAACAGCTTCTCCAGCT GCCAGCTGAGCCAGCAGCCCCTGGGCCCCGCTCACCCATTCAACCCCAGCCTGGCGGCCCCAGCCACCAA CCACATAGACCCTTGTGCTGTGGCCCCGGGGCCCCCGTCCGTGGTGGTGAAGCAGGAAGCCTTGCAGCCT GAGCCCGAGCCGGTCCCCGCCCCCCAGTTGCTTCTGGGGCCTCAGGGCCCCAGCCTCATCAAGGGGGTTG CACCTCCCACCCTCATCACCGACTCCACAGGGACCCACCTTGTCCTCACCGTGACCAATAAGAATGCAGA CAGCCCTGGCCTGTCCAGTGGGAGCCCCCAGCAGCCCTCGTCCCAGCCTGGCTCTCCAGCGCCTGCCCCC TCTGCCCAGATGGACCTGGAGCACCCACTGCAGCCCCTCTTTGGGACCCCCACTTCTCTGCTGAAGAAGG AACCACCTGGCTATGAGGAAGCCATGAGCCAGCAGCCCAAACAGCAGGAAAATGGTTCCTCAAGCCAGCA GATGGACGACCTGTTTGACATTCTCATTCAGAGCGGAGAAATTTCAGCAGATTTCAAGGAGCCGCCATCC CTGCCAGGGAAGGAGAAGCCATCCCCGAAGACAGTCTGTGGGTCCCCCCTGGCAGCACAGCCATCACCTT CTGCTGAGCTCCCCCAGGCTGCCCCACCTCCTCCAGGCTCACCCTCCCTCCCTGGACGCCTGGAGGACTT CCTGGAGAGCAGCACGGGGCTGCCCCTGCTGACCAGTGGGCATGACGGGCCAGAGCCCCTTTCCCTCATT GACGACCTCCATAGCCAGATGCTGAGCAGCACTGCCATCCTGGACCACCCCCCGTCACCCATGGACACCT CGGAATTGCACTTTGTTCCTGAGCCCAGCAGCACCATGGGCCTGGACCTGGCTGATGGCCACCTGGACAG CATGGACTGGCTGGAGCTGTCGTCAGGTGGTCCCGTGCTGAGCCTAGCCCCCCTCAGCACCACAGCCCCC AGCCTCTTCTCCACAGACTTCCTCGATGGCCATGATTTGCAGCTGCACTGGGATTCCTGCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221212 representing NM_020831
Red=Cloning site Green=Tags(s) MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADDL NEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSP LEARVSEPLLSATSASPTQVVSQLPMGRDSREMLFLAEQPPLPPPPLLPPSLTNGTTIPTAKSTPTLIKQ SQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILNQQQQ QHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTTNSSSSSGAPGPCGLARQNSTSLTGKPGALPANLDDM KVAELKQELKLRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAARLSTGPA LVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSPLTQLTLQ ASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLKLQLEQEKR AQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSVVVKQEALQP EPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPGLSSGSPQQPSSQPGSPAPAP SAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQMDDLFDILIQSGEISADFKEPPS LPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSPSLPGRLEDFLESSTGLPLLTSGHDGPEPLSLI DDLHSQMLSSTAILDHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAP SLFSTDFLDGHDLQLHWDSCL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020831 |
| ORF Size | 2793 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020831.4, NP_065882.1 |
| RefSeq Size | 4507 bp |
| RefSeq ORF | 3096 bp |
| Locus ID | 57591 |
| UniProt ID | Q969V6 |
| Domains | SAP, RPEL |
| Protein Families | Transcription Factors |
| Gene Summary | The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221212 | MKL1 (Myc-DDK-tagged)-Human megakaryoblastic leukemia (translocation) 1 (MKL1) |
CNY 10,224.00 |
|
| RC221212L3 | Lenti ORF clone of Human megakaryoblastic leukemia (translocation) 1 (MKL1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221212L4 | Lenti ORF clone of Human megakaryoblastic leukemia (translocation) 1 (MKL1), mGFP tagged |
CNY 9,030.00 |
|
| SC314943 | MKL1 (untagged)-Human megakaryoblastic leukemia (translocation) 1 (MKL1) |
CNY 18,050.00 |
