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Smek1 (PPP4R3A) (NM_032560) Human Tagged ORF Clone
CAT＃: RG221040
- TrueORF®
SMEK1 (tGFP-tagged) - Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1)
ORF Plasmid: DDK
"NM_032560" in other vectors (4)
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CNY 7,608.00
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FLFL1; KIAA2010; MSTP033; PP4R3A; smk-1; smk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221040 representing NM_032560
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGCA CCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGA CGGTTCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACAACAGGACACTCTGATT GTGTGGTCTGAAGCAGAAAATTATGACTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTT GGGAGAAAATATGTCAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGATGAATC TGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAATTGCCATCTTGTGAATTAAGTCGCCTT GAAGAAATTGCAGAACTTGTGGCATCATCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTGGCAC TAGAAAATGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGTGTGTGAAGATTTGGAAAATATTGA AGGACTGCACCACTTGTATGAAATTATCAAAGGCATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTT ATGTTCTCTGAAGAATGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCACAACCAC GAAAACACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAAGTGATTCCCATATCAGATCCTGAGCT GAAACAAAAAATTCATCAGACATACAGAGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTCGGTC TTTGAAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCAATAAGGTAGAGATTGTTGGCATGT TGCAGGAAGATGAAAAATTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAA AAGACAGGAATTGGTTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGA GATGCTTTTTTCAAGACTTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATG ATACACAGGTGCGAAGTGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACG AGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGATATTTTGCTCATCAACCTCATTATAGAACAT ATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTTATGGGCCTGCTACGAACTTTAGTTG ACCCAGAGAACATGCTAGCCACTGCCAATAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCA CTGTATGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGACAAACCTAGTAAAGATGATTTT CAGACTGCCCAACTATTGGCACTTGTATTGGAATTGTTAACATTTTGTGTGGAGCACCATACCTACCACA TAAAGAACTACATTATTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCATGCTTT CTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATTAAAAGATGAGTTTTACAACCGCTAC ATAATGAAAAGTTTTTTGTTTGAACCAGTAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATCTGA TGAACTCTGCCATAATAGAGATGTTTGAATTTATTAGGGTGGAAGATATAAAATCATTAACTGCTCATGT AATTGAAAATTACTGGAAAGCACTGGAAGATGTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGA TTTGAACAACAAAGAGAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCACA GATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGTGGTTTAACACAGATGAAGATGACAT GGAAGATGGAGAAGCTGTAGTGTCTCCATCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATA AGTAAATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAAGTGCTTCTGAAAACAAACCTTT CTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTGTCCAGTGGAACGAAGACTAACCTCACCAGCCAGTC ATCTACAACAAATCTGCCTGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGATCC GTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGAGGCCTCGTGGGTCTGGTAGATTATC CTGACGATGATGAAGATGATGATGAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAA ATTTGATTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221040 representing NM_032560
Red=Cloning site Green=Tags(s) MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLI VWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRL EEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEV MFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSV FEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNR DAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEH MICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDF QTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRY IMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLR FEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPI SKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGS VPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032560 |
| ORF Size | 2460 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032560.3, NP_115949.3 |
| RefSeq Size | 4449 bp |
| RefSeq ORF | 2462 bp |
| Locus ID | 55671 |
| Domains | DUF625 |
| Gene Summary | Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221040 | SMEK1 (Myc-DDK-tagged)-Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1) |
CNY 6,008.00 |
|
| RC221040L3 | Lenti ORF clone of Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1), Myc-DDK-tagged |
CNY 10,070.00 |
|
| RC221040L4 | Lenti ORF clone of Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1), mGFP tagged |
CNY 10,070.00 |
|
| SC104547 | SMEK1 (untagged)-Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1) |
CNY 6,016.00 |
