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Collagen XVII (COL17A1) (NM_000494) Human Tagged ORF Clone
CAT＃: RG220502
- TrueORF®
COL17A1 (tGFP-tagged) - Human collagen, type XVII, alpha 1 (COL17A1)
ORF Plasmid: DDK
"NM_000494" in other vectors (4)
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CNY 11,840.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BA16H23.2; BP180; BPA-2; BPAG2; EBR2A; ERED; JEB-I; LAD-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220502 representing NM_000494
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTAACCAAGAAAAACAAACGAGATGGAACTGAAGTCACTGAGAGAATTGTCACTGAAACAGTAA CCACAAGACTTACATCCTTACCACCAAAAGGCGGGACCAGCAATGGCTATGCTAAAACAGCCTCTCTTGG TGGAGGGAGCCGGCTGGAGAAACAAAGCCTGACTCATGGCAGCAGCGGCTACATAAACTCAACTGGAAGC ACACGAGGCCATGCCTCCACCTCTAGTTACAGGAGGGCTCACTCACCTGCCTCCACTCTGCCCAACTCCC CAGGCTCAACCTTTGAAAGGAAAACTCACGTTACCCGCCATGCGTATGAAGGGAGCTCCAGTGGCAACTC TTCTCCGGAGTACCCTCGGAAGGAATTTGCATCTTCTTCAACCAGAGGACGGAGTCAAACACGAGAGAGT GAAATTCGAGTTCGACTGCAGAGTGCGTCCCCATCCACCCGATGGACAGAATTGGATGATGTTAAGCGTT TGCTCAAGGGGAGTCGATCGGCAAGTGTGAGCCCCACCCGGAATTCCTCCAACACACTCCCCATCCCCAA GAAAGGCACTGTGGAGACCAAAATTGTGACAGCGAGCTCCCAGTCGGTGTCAGGCACCTACGATGCAATG ATCCTGGATGCCAACCTTCCCTCCCATGTGTGGTCCTCCACCCTGCCCGCGGGGTCCTCCATGGGGACCT ATCACAACAACATGACAACCCAGAGCTCATCCCTCCTCAACACCAATGCCTACTCTGCGGGATCAGTCTT CGGAGTTCCAAACAACATGGCGTCCTGCTCACCCACTTTGCACCCTGGACTCAGCACATCCTCCTCAGTG TTTGGCATGCAGAACAATCTGGCCCCCAGCTTGACCACCCTGTCCCATGGCACCACCACCACTTCCACAG CATATGGGGTGAAGAAAAACATGCCCCAGAGTCCTGCGGCTGTGAACACTGGCGTTTCCACCTCCGCCGC CTGCACCACAAGTGTGCAGAGCGATGACCTTTTGCACAAGGACTGCAAGTTCCTGATCCTAGAGAAAGAC AACACACCTGCTAAGAAGGAGATGGAGCTGCTCATCATGACCAAGGACAGCGGGAAGGTCTTTACAGCCT CCCCTGCCAGCATCGCTGCAACTTCTTTTTCAGAAGACACCCTAAAAAAAGAAAAGCAAGCTGCCTACAA TGCTGACTCAGGCCTAAAAGCCGAAGCTAATGGAGACCTGAAGACTGTGTCCACAAAGGGCAAGACCACC ACTGCAGATATCCACAGCTACAGCAGCAGTGGTGGTGGTGGCAGTGGAGGAGGTGGCGGTGTTGGTGGCG CTGGCGGCGGCCCTTGGGGACCAGCGCCAGCCTGGTGCCCCTGCGGCTCCTGCTGCAGCTGGTGGAAGTG GCTGCTGGGCCTGCTGCTCACCTGGCTGCTACTCCTGGGGCTGCTCTTCGGCCTCATTGCTCTGGCGGAG GAGGTGAGGAAGCTGAAGGCGCGTGTGGATGAGCTGGAGAGGATCAGGAGGAGCATACTGCCCTATGGGG ACAGCATGGATAGAATAGAAAAGGACCGCCTCCAGGGCATGGCACCCGCGGCGGGAGCAGACCTGGACAA AATTGGGCTGCACAGTGACAGCCAGGAGGAGCTCTGGATGTTCGTGAGGAAGAAGCTAATGATGGAACAG GAAAATGGAAATCTCCGAGGAAGCCCTGGCCCTAAAGGTGACATGGGAAGTCCAGGCCCTAAAGGAGATC GAGGGTTCCCTGGGACTCCAGGTATCCCTGGGCCCTTGGGCCACCCAGGTCCACAAGGACCAAAGGGTCA AAAAGGCAGCGTGGGAGATCCTGGCATGGAAGGCCCCATGGGCCAGAGAGGGCGAGAAGGCCCCATGGGA CCTCGTGGTGAGGCAGGGCCTCCTGGATCTGGAGAGAAAGGGGAAAGAGGGGCTGCTGGTGAACCAGGTC CTCATGGCCCACCTGGTGTCCCAGGTTCTGTGGGTCCCAAAGGTTCCAGCGGCTCTCCTGGCCCACAGGG CCCTCCAGGTCCTGTAGGTCTCCAAGGGCTCCGAGGTGAAGTAGGACTTCCTGGTGTCAAAGGTGACAAA GGACCAGTGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAAGGACCTCGAGGCCTCACAGGCGAGC CTGGCATGAGAGGTTTGCCTGGTGCTGTTGGTGAGCCCGGGGCTAAAGGAGCAATGGGTCCTGCTGGCCC AGACGGACACCAAGGCCCAAGAGGTGAACAAGGTCTTACTGGGATGCCTGGAATCCGTGGCCCACCAGGA CCTTCTGGAGACCCAGGAAAGCCAGGTCTCACAGGACCCCAGGGACCTCAGGGACTTCCCGGTACCCCTG GCCGACCAGGAATAAAAGGTGAACCAGGAGCTCCAGGCAAGATCGTGACTTCGGAGGGGTCATCGATGCT CACTGTCCCAGGCCCCCCAGGACCTCCTGGAGCCATGGGACCCCCAGGACCTCCAGGTGCCCCAGGCCCT GCCGGCCCAGCTGGTCTCCCAGGACATCAAGAAGTTCTTAATTTACAAGGTCCCCCAGGCCCACCCGGCC CACGCGGGCCACCAGGGCCTTCCATTCCAGGCCCACCAGGACCCCGAGGCCCACCAGGGGAGGGTTTGCC AGGCCCACCAGGCCCACCAGGATCGTTCCTGTCCAACTCAGAAACCTTCCTCTCCGGCCCCCCAGGCCCA CCTGGCCCCCCAGGTCCCAAGGGAGACCAAGGTCCCCCAGGCCCCAGAGGACACCAAGGCGAGCAAGGCC TCCCAGGTTTCTCAACCTCAGGGTCCAGTTCTTTCGGACTCAACCTTCAGGGACCACCAGGCCCACCTGG CCCCCAGGGACCAAAAGGTGACAAAGGTGATCCGGGTGTTCCAGGGGCTCTTGGCATTCCTAGTGGTCCT TCTGAAGGGGGATCATCAAGTACCATGTACGTGTCAGGCCCGCCAGGGCCCCCTGGGCCCCCTGGGCCTC CGGGCTCTATCAGCAGCTCTGGCCAGGAGATTCAGCAGTACATCTCTGAGTACATGCAGAGTGACAGTAT TAGATCTTACCTATCCGGAGTTCAGGGTCCCCCAGGCCCACCTGGTCCCCCAGGACCTGTCACCACCATC ACAGGCGAGACTTTCGACTACTCAGAGCTGGCAAGCCACGTTGTGAGCTACTTACGGACTTCGGGGTACG GTGTCAGCTTGTTCTCGTCCTCCATCTCTTCTGAAGACATCCTGGCTGTGCTGCAGCGGGATGACGTGCG TCAGTACCTACGTCAGTACTTGATGGGCCCTCGGGGTCCGCCAGGGCCACCAGGAGCCAGTGGAGATGGG TCCCTCCTGTCTTTGGACTATGCAGAGCTGAGTAGTCGCATTCTCAGCTACATGTCGAGTTCTGGGATCA GCATTGGGCTTCCTGGTCCCCCGGGGCCCCCTGGCTTGCCGGGAACCTCCTATGAGGAGCTCCTCTCCTT GCTGCGAGGGTCTGAATTCAGAGGCATCGTTGGACCCCCAGGTCCCCCGGGTCCACCAGGGATCCCAGGC AATGTGTGGTCCAGCATCAGCGTGGAGGACCTCTCGTCTTACTTACATACTGCCGGCTTGTCATTCATCC CAGGCCCTCCAGGACCTCCTGGTCCCCCAGGGCCTCGAGGGCCCCCGGGTGTCTCAGGAGCCCTGGCAAC CTATGCAGCTGAAAACAGCGACAGCTTCCGGAGCGAGCTGATCAGCTACCTCACAAGTCCTGATGTGCGC AGCTTCATTGTTGGCCCCCCAGGCCCTCCTGGGCCGCAGGGACCCCCTGGGGACAGCCGCCTCCTGTCCA CGGATGCCTCCCACAGTCGGGGTAGCAGCTCCTCCTCACACAGCTCATCTGTCAGGCGGGGCAGCTCCTA CAGCTCTTCCATGAGCACAGGAGGAGGTGGTGCAGGCTCCCTGGGTGCAGGCGGTGCCTTTGGTGAAGCT GCAGGAGACAGGGGTCCCTATGGCACTGACATCGGCCCAGGCGGAGGCTATGGGGCAGCAGCAGAAGGCG GCATGTATGCTGGCAATGGCGGACTATTGGGAGCTGACTTTGCTGGAGATCTGGATTACAATGAGCTGGC TGTGAGGGTGTCAGAGAGCATGCAGCGTCAGGGCCTACTGCAAGGGATGGCCTACACTGTCCAGGGCCCA CCAGGCCAGCCTGGGCCACAGGGGCCACCCGGCATCAGCAAGGTCTTCTCTGCCTACAGCAACGTGACTG CGGACCTCATGGACTTCTTCCAAACTTATGGAGCCATTCAAGGACCCCCTGGGCAAAAAGGAGAGATGGG CACTCCAGGACCCAAAGGTGACAGGGGCCCTGCTGGGCCACCAGGTCATCCTGGGCCACCTGGCCCTCGA GGACACAAGGGAGAAAAAGGAGACAAAGGTGACCAAGTCTATGCTGGGCGGAGAAGGAGAAGAAGTATTG CTGTCAAGCCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220502 representing NM_000494
Red=Cloning site Green=Tags(s) MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGS TRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRES EIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASSQSVSGTYDAM ILDANLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTNAYSAGSVFGVPNNMASCSPTLHPGLSTSSSV FGMQNNLAPSLTTLSHGTTTTSTAYGVKKNMPQSPAAVNTGVSTSAACTTSVQSDDLLHKDCKFLILEKD NTPAKKEMELLIMTKDSGKVFTASPASIAATSFSEDTLKKEKQAAYNADSGLKAEANGDLKTVSTKGKTT TADIHSYSSSGGGGSGGGGGVGGAGGGPWGPAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAE EVRKLKARVDELERIRRSILPYGDSMDRIEKDRLQGMAPAAGADLDKIGLHSDSQEELWMFVRKKLMMEQ ENGNLRGSPGPKGDMGSPGPKGDRGFPGTPGIPGPLGHPGPQGPKGQKGSVGDPGMEGPMGQRGREGPMG PRGEAGPPGSGEKGERGAAGEPGPHGPPGVPGSVGPKGSSGSPGPQGPPGPVGLQGLRGEVGLPGVKGDK GPVGPPGPKGDQGEKGPRGLTGEPGMRGLPGAVGEPGAKGAMGPAGPDGHQGPRGEQGLTGMPGIRGPPG PSGDPGKPGLTGPQGPQGLPGTPGRPGIKGEPGAPGKIVTSEGSSMLTVPGPPGPPGAMGPPGPPGAPGP AGPAGLPGHQEVLNLQGPPGPPGPRGPPGPSIPGPPGPRGPPGEGLPGPPGPPGSFLSNSETFLSGPPGP PGPPGPKGDQGPPGPRGHQGEQGLPGFSTSGSSSFGLNLQGPPGPPGPQGPKGDKGDPGVPGALGIPSGP SEGGSSSTMYVSGPPGPPGPPGPPGSISSSGQEIQQYISEYMQSDSIRSYLSGVQGPPGPPGPPGPVTTI TGETFDYSELASHVVSYLRTSGYGVSLFSSSISSEDILAVLQRDDVRQYLRQYLMGPRGPPGPPGASGDG SLLSLDYAELSSRILSYMSSSGISIGLPGPPGPPGLPGTSYEELLSLLRGSEFRGIVGPPGPPGPPGIPG NVWSSISVEDLSSYLHTAGLSFIPGPPGPPGPPGPRGPPGVSGALATYAAENSDSFRSELISYLTSPDVR SFIVGPPGPPGPQGPPGDSRLLSTDASHSRGSSSSSHSSSVRRGSSYSSSMSTGGGGAGSLGAGGAFGEA AGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGP PGQPGPQGPPGISKVFSAYSNVTADLMDFFQTYGAIQGPPGQKGEMGTPGPKGDRGPAGPPGHPGPPGPR GHKGEKGDKGDQVYAGRRRRRSIAVKP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000494 |
| ORF Size | 4491 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000494.2, NP_000485.2 |
| RefSeq Size | 5610 bp |
| RefSeq ORF | 4494 bp |
| Locus ID | 1308 |
| UniProt ID | Q9UMD9 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220502 | COL17A1 (Myc-DDK-tagged)-Human collagen, type XVII, alpha 1 (COL17A1) |
CNY 10,240.00 |
|
| RC220502L3 | Lenti ORF clone of Human collagen, type XVII, alpha 1 (COL17A1), Myc-DDK-tagged |
CNY 16,820.00 |
|
| RC220502L4 | Lenti ORF clone of Human collagen, type XVII, alpha 1 (COL17A1), mGFP tagged |
CNY 12,640.00 |
|
| SC300082 | COL17A1 (untagged)-Human collagen, type XVII, alpha 1 (COL17A1) |
CNY 10,248.00 |
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