MR1 (NM_001531) Human Tagged ORF Clone
CAT#: RG220474
- TrueORF®
MR1 (tGFP-tagged) - Human major histocompatibility complex, class I-related (MR1), transcript variant 1
ORF Plasmid: DDK
"NM_001531" in other vectors (4)
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CNY 5,256.00
Cited in 3 publications. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HLALS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG220474 representing NM_001531
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAACTGATGGCGTTCCTGTTACCTCTCATCATTGTGTTAATGGTGAAGCACAGCGATTCCCGGA CGCACTCTCTGAGATATTTTCGCCTGGGCGTTTCGGATCCCATCCATGGGGTCCCTGAATTTATTTCGGT TGGGTACGTGGACTCGCACCCTATCACCACATATGACAGTGTCACTCGGCAGAAGGAGCCACGGGCCCCA TGGATGGCAGAGAACCTCGCGCCTGATCACTGGGAGAGGTACACTCAGCTGCTGAGGGGCTGGCAGCAGA TGTTCAAGGTGGAACTGAAGCGCCTACAGAGGCACTACAATCACTCAGGGTCTCACACTTACCAGAGAAT GATTGGCTGTGAGCTGCTGGAGGATGGAAGCACCACAGGATTTCTGCAGTATGCATATGACGGGCAGGAT TTCCTGATCTTCAATAAAGACACCCTCTCCTGGCTGGCTGTAGATAATGTGGCTCACACCATCAAGCAGG CATGGGAGGCCAATCAGCATGAGTTGCTGTATCAAAAGAATTGGCTGGAAGAAGAATGTATTGCCTGGCT AAAGAGATTCCTGGAGTATGGGAAAGACACCCTACAAAGAACAGAGCCCCCACTGGTCAGAGTAAATCGC AAAGAAACTTTTCCAGGGGTTACAGCTCTCTTCTGCAAAGCTCATGGCTTTTACCCCCCAGAAATTTACA TGACATGGATGAAAAACGGGGAAGAAATTGTCCAAGAAATTGATTATGGAGACATTCTTCCCAGTGGGGA TGGAACCTATCAGGCGTGGGCATCAATTGAGCTTGATCCTCAGAGCAGCAACCTTTACTCCTGTCATGTG GAGCACTGCGGTGTCCACATGGTTCTTCAGGTCCCCCAGGAATCAGAAACTATCCCTCTTGTGATGAAAG CTGTCTCTGGGTCCATTGTCCTTGTCATTGTGCTGGCTGGAGTTGGTGTTCTAGTCTGGAGAAGAAGGCC CCGAGAGCAAAATGGAGCCATCTACCTTCCAACACCAGATCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220474 representing NM_001531
Red=Cloning site Green=Tags(s) MGELMAFLLPLIIVLMVKHSDSRTHSLRYFRLGVSDPIHGVPEFISVGYVDSHPITTYDSVTRQKEPRAP WMAENLAPDHWERYTQLLRGWQQMFKVELKRLQRHYNHSGSHTYQRMIGCELLEDGSTTGFLQYAYDGQD FLIFNKDTLSWLAVDNVAHTIKQAWEANQHELLYQKNWLEEECIAWLKRFLEYGKDTLQRTEPPLVRVNR KETFPGVTALFCKAHGFYPPEIYMTWMKNGEEIVQEIDYGDILPSGDGTYQAWASIELDPQSSNLYSCHV EHCGVHMVLQVPQESETIPLVMKAVSGSIVLVIVLAGVGVLVWRRRPREQNGAIYLPTPDR TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001531 |
ORF Size | 1023 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001531.2 |
RefSeq Size | 1263 bp |
RefSeq ORF | 1026 bp |
Locus ID | 3140 |
UniProt ID | Q95460 |
Domains | MHC_I, IGc1 |
Protein Families | Transmembrane |
Gene Summary | MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome. [provided by RefSeq, Jul 2015] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Tuning of human MAIT cell activation by commensal bacteria species and MR1 dependent T cell presentation
,null,
Mucosal immunology
,PubMed ID 30115998
[MR1]
|
Endosomal MR1 Trafficking Plays a Key Role in Presentation of Mycobacterium tuberculosis Ligands to MAIT Cells
,Harriff, MJ;Karamooz, E;Burr, A;Grant, WF;Canfield, ET;Sorensen, ML;Moita, LF;Lewinsohn, DM;,
PLoS Pathog.
,PubMed ID 27031111
[MR1]
|
The Role of Rab6 in Presenting Mycobacterium Tuberculosis Antigens to CD8+ T Cells from Infected Epithelial Cells
,Siekavizza-Robles, CR;,
Thesis
[MR1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220474 | MR1 (Myc-DDK-tagged)-Human major histocompatibility complex, class I-related (MR1), transcript variant 1 |
CNY 3,656.00 |
|
RC220474L3 | Lenti ORF clone of Human major histocompatibility complex, class I-related (MR1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC220474L4 | Lenti ORF clone of Human major histocompatibility complex, class I-related (MR1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
SC119190 | MR1 (untagged)-Human major histocompatibility complex, class I-related (MR1), transcript variant 1 |
CNY 3,656.00 |