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RPGR (NM_000328) Human Tagged ORF Clone
CAT＃: RG220424
- TrueORF®
RPGR (tGFP-tagged) - Human retinitis pigmentosa GTPase regulator (RPGR), transcript variant A
ORF Plasmid: DDK
"NM_000328" in other vectors (4)
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CNY 7,568.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | COD1; CORDX1; CRD; orf15; PCDX; RP3; RP15; XLRP3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220424 representing NM_000328
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGAGCCGGAAGAGCTGATGCCCGATTCGGGTGCTGTGTTTACATTTGGGAAAAGTAAATTTGCTG AAAATAATCCCGGTAAATTCTGGTTTAAAAATGATGTCCCTGTACATCTTTCATGTGGAGATGAACATTC TGCTGTTGTTACCGGAAATAATAAACTTTACATGTTTGGCAGTAACAACTGGGGTCAGTTAGGATTAGGA TCAAAGTCAGCCATCAGCAAGCCAACATGTGTCAAAGCTCTAAAACCTGAAAAAGTGAAATTAGCTGCCT GTGGAAGGAACCACACCCTGGTGTCAACAGAAGGAGGCAATGTATATGCAACTGGTGGAAATAATGAAGG ACAGTTGGGGCTTGGTGACACCGAAGAAAGAAACACTTTTCATGTAATTAGCTTTTTTACATCCGAGCAT AAGATTAAGCAGCTGTCTGCTGGATCTAATACTTCAGCTGCCCTAACTGAGGATGGAAGACTTTTTATGT GGGGTGACAATTCCGAAGGGCAAATTGGTTTAAAAAATGTAAGTAATGTCTGTGTCCCTCAGCAAGTGAC CATTGGGAAACCTGTCTCCTGGATCTCTTGTGGATATTACCATTCAGCTTTTGTAACAACAGATGGTGAG CTATATGTGTTTGGAGAACCTGAGAATGGGAAGTTAGGTCTTCCCAATCAGCTCCTGGGCAATCACAGAA CACCCCAGCTGGTGTCTGAAATTCCGGAGAAGGTGATCCAAGTAGCCTGTGGTGGAGAGCATACTGTGGT TCTCACGGAGAATGCTGTGTATACCTTTGGGCTGGGACAATTTGGTCAGCTGGGTCTTGGCACTTTTCTT TTTGAAACTTCAGAACCCAAAGTCATTGAGAATATTAGGGATCAAACAATAAGTTATATTTCTTGTGGAG AAAATCACACAGCTTTGATAACAGATATCGGCCTTATGTATACTTTTGGAGATGGTCGCCACGGAAAATT AGGACTTGGACTGGAGAATTTTACCAATCACTTCATTCCTACTTTGTGCTCTAATTTTTTGAGGTTTATA GTTAAATTGGTTGCTTGTGGTGGATGTCACATGGTAGTTTTTGCTGCTCCTCATCGTGGTGTGGCAAAAG AAATTGAATTCGATGAAATAAATGATACTTGCTTATCTGTGGCGACTTTTCTGCCGTATAGCAGTTTAAC CTCAGGAAATGTACTGCAGAGGACTCTATCAGCACGTATGCGGCGAAGAGAGAGGGAGAGGTCTCCAGAT TCTTTTTCAATGAGGAGAACACTACCTCCAATAGAAGGGACTCTTGGCCTTTCTGCTTGTTTTCTCCCCA ATTCAGTCTTTCCACGATGTTCTGAGAGAAACCTCCAAGAGAGTGTCTTATCTGAACAGGACCTCATGCA GCCAGAGGAACCAGATTATTTGCTAGATGAAATGACCAAAGAAGCAGAGATAGATAATTCTTCAACTGTA GAAAGCCTTGGAGAAACTACTGATATCTTAAACATGACACACATCATGAGCCTGAATTCCAATGAAAAGT CATTAAAATTATCACCAGTTCAGAAACAAAAGAAACAACAAACAATTGGGGAACTGACGCAGGATACAGC TCTTACTGAAAACGATGATAGTGATGAATATGAAGAAATGTCAGAAATGAAAGAAGGGAAAGCATGTAAA CAACATGTGTCACAAGGGATTTTCATGACGCAGCCAGCTACGACTATCGAAGCATTTTCAGATGAGGAAG TAGAGATCCCAGAGGAGAAGGAAGGAGCAGAGGATTCAAAAGGAAATGGAATAGAGGAGCAAGAGGTAGA AGCAAATGAGGAAAATGTGAAGGTGCATGGAGGAAGAAAGGAGAAAACAGAGATCCTATCAGATGACCTT ACAGACAAAGCAGAGGATCATGAATTTTCTAAAACTGAGGAACTAAAACTAGAAGATGTGGATGAGGAAA TTAATGCTGAAAATGTGGAAAGCAAGAAGAAAACTGTGGGAGATGATGAAAGTGTTCCTACAGGTTATCA CAGTAAAACAGAAGGAGCAGAAAGAACCAATGATGATAGCTCAGCTGAAACTATTGAAAAGAAAGAAAAA GCCAACCTAGAGGAACGGGCCATTTGTGAGTACAATGAAAACCCAAAAGGATACATGCTTGATGATGCAG ATAGCAGTTCATTAGAAATCCTAGAAAACAGTGAAACAACACCAAGCAAAGACATGAAAAAAACAAAGAA GATTTTTCTGTTCAAAAGAGTCCCCTCAATAAATCAAAAGATTGTCAAGAATAACAATGAGCCGCTCCCA GAGATAAAATCCATAGGAGACCAGATCATTTTAAAAAGTGATAATAAAGATGCCGACCAGAACCACATGA GTCAGAATCATCAGAATATCCCACCAACAAATACAGAGAGAAGATCAAAATCCTGTACAATACTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220424 representing NM_000328
Red=Cloning site Green=Tags(s) MREPEELMPDSGAVFTFGKSKFAENNPGKFWFKNDVPVHLSCGDEHSAVVTGNNKLYMFGSNNWGQLGLG SKSAISKPTCVKALKPEKVKLAACGRNHTLVSTEGGNVYATGGNNEGQLGLGDTEERNTFHVISFFTSEH KIKQLSAGSNTSAALTEDGRLFMWGDNSEGQIGLKNVSNVCVPQQVTIGKPVSWISCGYYHSAFVTTDGE LYVFGEPENGKLGLPNQLLGNHRTPQLVSEIPEKVIQVACGGEHTVVLTENAVYTFGLGQFGQLGLGTFL FETSEPKVIENIRDQTISYISCGENHTALITDIGLMYTFGDGRHGKLGLGLENFTNHFIPTLCSNFLRFI VKLVACGGCHMVVFAAPHRGVAKEIEFDEINDTCLSVATFLPYSSLTSGNVLQRTLSARMRRRERERSPD SFSMRRTLPPIEGTLGLSACFLPNSVFPRCSERNLQESVLSEQDLMQPEEPDYLLDEMTKEAEIDNSSTV ESLGETTDILNMTHIMSLNSNEKSLKLSPVQKQKKQQTIGELTQDTALTENDDSDEYEEMSEMKEGKACK QHVSQGIFMTQPATTIEAFSDEEVEIPEEKEGAEDSKGNGIEEQEVEANEENVKVHGGRKEKTEILSDDL TDKAEDHEFSKTEELKLEDVDEEINAENVESKKKTVGDDESVPTGYHSKTEGAERTNDDSSAETIEKKEK ANLEERAICEYNENPKGYMLDDADSSSLEILENSETTPSKDMKKTKKIFLFKRVPSINQKIVKNNNEPLP EIKSIGDQIILKSDNKDADQNHMSQNHQNIPPTNTERRSKSCTIL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000328 |
| ORF Size | 2445 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000328.3 |
| RefSeq Size | 3082 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 6103 |
| UniProt ID | Q92834 |
| Domains | RCC1 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. [provided by RefSeq, Dec 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220424 | RPGR (Myc-DDK-tagged)-Human retinitis pigmentosa GTPase regulator (RPGR), transcript variant A |
CNY 5,968.00 |
|
| RC220424L3 | Lenti ORF clone of Human retinitis pigmentosa GTPase regulator (RPGR), transcript variant A, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC220424L4 | Lenti ORF clone of Human retinitis pigmentosa GTPase regulator (RPGR), transcript variant A, mGFP tagged |
CNY 7,700.00 |
|
| SC119976 | RPGR (untagged)-Human retinitis pigmentosa GTPase regulator (RPGR), transcript variant A |
CNY 5,976.00 |
