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BAZ2A (NM_013449) Human Tagged ORF Clone
CAT＃: RG220374
- TrueORF®
BAZ2A (tGFP-tagged) - Human bromodomain adjacent to zinc finger domain, 2A (BAZ2A)
ORF Plasmid: DDK
"NM_013449" in other vectors (2)
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CNY 15,200.00
货期*
4周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TIP5; WALp3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220374 representing NM_013449
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATGGAGGCAAACGACCATTTTAACTTTACTGGCCTTCCCCCTGCACCTGCTGCCTCAGGACTGA AACCCTCTCCTTCCTCAGGGGAGGGCCTCTACACTAACGGGTCTCCCATGAACTTCCCCCAGCAAGGGAA AAGTTTGAATGGGGATGTGAATGTTAATGGCTTATCTACTGTATCTCACACTACTACTTCAGGGATTTTG AACTCTGCTCCCCACTCCTCCAGCACCTCACACCTCCATCACCCCAGCGTGGCCTACGACTGTCTCTGGA ACTACTCACAGTACCCATCTGCCAATCCTGGCAGCAACCTCAAGGACCCACCCCTTCTCTCCCAGTTCTC GGGGGGACAATACCCACTCAACGGCATCCTTGGGGGCAGCCGGCAACCTTCATCCCCAAGTCATAACACT AACCTTCGGGCTGGGAGCCAAGAGTTCTGGGCCAACGGTACCCAGAGTCCCATGGGGCTTAACTTTGATT CACAAGAACTGTATGATTCCTTTCCTGACCAGAATTTTGAGGTGATGCCCAATGGACCCCCTAGTTTTTT CACCTCCCCACAGACTTCTCCTATGTTGGGATCTAGCATTCAAACCTTTGCACCCTCCCAGGAGGTAGGC AGTGGTATCCATCCTGATGAGGCAGCAGAAAAGGAGATGACTTCAGTTGTGGCAGAGAATGGCACTGGCT TGGTAGGCAGCTTGGAGCTGGAAGAAGAGCAGCCAGAACTGAAGATGTGTGGCTACAATGGCTCTGTCCC TTCTGTGGAATCGTTACACCAAGAGGTCTCAGTCCTGGTCCCTGACCCCACAGTGAGCTGTTTAGATGAT CCTTCACATCTTCCTGATCAACTGGAAGACACTCCAATCCTCAGTGAAGACTCTCTGGAGCCCTTCAACT CTCTGGCACCAGAGCCAGTGAGTGGAGGACTATATGGTATTGATGACACGGAGCTGATGGGTGCAGAGGA CAAGCTGCCTCTTGAGGACAGCCCTGTGATTTCTGCCCTTGATTGCCCTTCCCTCAATAATGCTACTGCC TTCAGTCTCCTGGCAGATGATAGTCAAACATCAACCTCTATCTTTGCCAGTCCCACCTCTCCACCTGTCC TAGGGGAGTCTGTCCTGCAAGATAACAGCTTTGACCTGAATAATGGTAGTGACGCTGAACAGGAAGAAAT GGAAACTCAATCTTCAGACTTCCCACCATCCCTGACCCAGCCAGCTCCTGATCAGTCATCCACTATTCAG CTACATCCAGCAACCTCACCAGCAGTCTCGCCAACAACCTCCCCAGCAGTCTCCCTAGTGGTTTCTCCAG CAGCCTCCCCAGAAATCTCTCCAGAAGTTTGTCCCGCAGCTTCTACAGTTGTCTCTCCAGCAGTCTTCTC AGTGGTCTCTCCAGCTTCCTCAGCAGTCCTCCCAGCAGTCTCCTTAGAAGTCCCGTTGACGGCTTCAGTG ACATCCCCAAAAGCCTCTCCCGTAACTTCCCCAGCAGCTGCCTTTCCAACAGCCTCCCCAGCAAATAAGG ATGTCAGCAGCTTTCTAGAAACCACTGCTGACGTGGAAGAGATCACTGGAGAAGGACTCACTGCTTCTGG TAGTGGTGATGTCATGAGGAGACGTATTGCTACCCCAGAAGAAGTTCGTCTTCCCCTCCAACATGGGTGG CGGAGAGAGGTGCGCATCAAGAAGGGCAGCCACCGATGGCAGGGGGAGACCTGGTATTATGGCCCCTGTG GGAAGAGGATGAAGCAATTTCCAGAAGTGATCAAGTACCTGAGCCGCAACGTGGTACACAGTGTCCGCCG AGAGCACTTCAGCTTCAGTCCCCGTATGCCTGTTGGAGATTTCTTTGAAGAAAGAGACACGCCAGAGGGC TTGCAGTGGGTGCAGCTCTCAGCAGAGGAGATCCCGTCGAGGATTCAGGCAATTACTGGCAAACGGGGTC GACCTCGAAACACTGAGAAGGCTAAGACTAAGGAAGTCCCCAAGGTGAAACGGGGTCGAGGTCGGCCACC TAAGGTCAAAATCACTGAGCTATTGAACAAGACAGACAACCGCCCCCTAAAGAAACTGGAGGCCCAAGAA ACATTGAATGAGGAGGATAAAGCAAAGATTGCTAAAAGCAAGAAGAAGATGAGGCAGAAGGTTCAACGGG GAGAGTGTCAGACTACTATCCAAGGGCAGGCCAGAAATAAGCGGAAACAAGAGACCAAGAGCTTAAAGCA GAAGGAAGCTAAGAAGAAATCCAAGGCTGAGAAAGAAAAAGGAAAGACAAAGCAGGAAAAACTGAAGGAA AAAGTCAAGAGGGAAAAGAAGGAGAAGGTAAAAATGAAGGAAAAGGAGGAGGTGACCAAAGCCAAGCCAG CCTGTAAAGCAGATAAGACCCTGGCCACACAGAGGCGCTTGGAGGAACGGCAGAGGCAGCAGATGATCTT GGAGGAAATGAAGAAGCCGACAGAGGATATGTGTCTGACTGACCACCAGCCCCTGCCTGACTTCTCACGA GTCCCTGGTCTGACATTGCCCAGTGGAGCCTTCTCAGACTGCTTGACCATTGTGGAGTTCCTGCATAGCT TTGGCAAGGTGCTGGGCTTTGATCCTGCCAAAGATGTGCCTAGCCTGGGGGTCCTGCAGGAGGGACTCCT GTGTCAAGGTGACAGCTTGGGTGAGGTGCAAGACCTGCTGGTCAGGCTGCTGAAGGCTGCACTCCATGAT CCTGGCTTTCCCTCCTACTGTCAGTCCCTAAAGATCTTGGGGGAGAAGGTGTCTGAGATCCCACTGACAA GAGACAATGTGTCAGAGATCCTGCGCTGCTTCCTTATGGCATATGGAGTAGAGCCAGCCCTCTGTGACCG CCTGCGCACCCAGCCTTTTCAGGCCCAGCCACCCCAGCAGAAGGCTGCTGTCCTGGCCTTCCTTGTGCAT GAGCTCAATGGCTCCACCCTCATCATCAATGAGATTGACAAGACTCTGGAGAGTATGTCCAGCTACAGGA AAAACAAGTGGATTGTTGAAGGCCGGCTCCGGAGGCTGAAAACTGTTCTGGCCAAGCGAACTGGGCGGTC TGAAGTAGAGATGGAAGGGCCAGAGGAATGCCTGGGACGGAGGCGCAGTTCTCGGATCATGGAGGAGACC AGTGGCATGGAAGAAGAGGAAGAAGAGGAGTCTATAGCAGCTGTCCCTGGCCGCAGGGGTCGAAGAGATG GAGAGGTTGATGCCACAGCATCTAGCATCCCAGAGCTAGAGCGCCAGATAGAAAAACTCAGCAAGCGTCA GCTTTTCTTTCGCAAAAAGCTGCTTCACTCATCCCAGATGCTTCGGGCGGTCTCCCTGGGTCAGGACCGC TACAGACGTCGCTACTGGGTATTGCCGTATTTGGCTGGTATCTTTGTAGAAGGAACAGAGGGGAACTTAG TTCCTGAGGAGGTGATAAAGAAGGAAACTGACTCCTTAAAAGTGGCAGCCCATGCGTCACTCAACCCTGC CCTCTTCTCTATGAAGATGGAGTTAGCTGGCTCCAACACCACTGCCAGTTCTCCTGCCCGGGCCCGAGGC CGACCTCGAAAAACTAAGCCCGGGTCTATGCAACCTAGGCATCTTAAGTCCCCTGTCAGGGGTCAGGATT CAGAACAGCCCCAGGCCCAGCTTCAGCCTGAGGCTCAGCTTCATGCTCCTGCCCAGCCCCAGCCTCAGCT TCAGCTTCAGCTTCAGTCCCATAAGGGGTTCCTGGAGCAAGAAGGCTCCCCTTTGTCACTGGGTCAGAGC CAGCATGACCTCAGCCAGTCAGCCTTCCTGTCTTGGCTGAGCCAGACTCAGAGCCATAGCTCCCTGTTGA GCAGCTCAGTCCTCACACCTGATAGCAGTCCGGGAAAACTAGACCCAGCTCCATCACAACCCCCGGAGGA GCCAGAGCCTGATGAGGCAGAATCCAGCCCTGATCCTCAAGCACTCTGGTTTAACATCTCAGCCCAGATG CCCTGCAATGCTGCCCCCACACCGCCCCCTGCAGTTTCTGAGGACCAACCCACTCCCTCCCCTCAGCAGC TTGCCTCCTCCAAGCCAATGAATAGACCTAGTGCTGCCAACCCTTGTTCTCCAGTGCAGTTCTCTTCCAC GCCCTTGGCTGGGTTGGCCCCTAAGAGGCGAGCAGGAGACCCTGGAGAAATGCCACAGAGTCCCACAGGG CTGGGACAGCCCAAACGGAGAGGGAGACCTCCCAGTAAGTTCTTCAAACAGATGGAACAGCGTTACCTAA CCCAGCTGACAGCCCAGCCTGTCCCACCTGAGATGTGCTCAGGCTGGTGGTGGATACGAGATCCTGAGAT GTTGGATGCCATGCTCAAGGCCCTACACCCCCGAGGTATCCGGGAGAAGGCACTTCACAAACACCTTAAC AAGCACAGGGACTTCTTGCAGGAAGTCTGCCTGCGGCCCTCAGCTGACCCCATCTTTGAGCCCAGGCAAC TACCTGCCTTTCAAGAAGGGATTATGAGCTGGTCCCCCAAAGAGAAGACATACGAGACAGACCTAGCAGT GCTTCAATGGGTAGAGGAGCTGGAGCAGCGGGTTATCATGTCTGATCTGCAGATTCGGGGCTGGACATGT CCTAGCCCAGACTCTACCCGTGAAGACTTGGCCTACTGTGAGCACCTCTCCGACTCCCAGGAGGATATCA CCTGGCGAGGTCGGGGCAGGGAGGGACTGGCACCTCAGCGTAAAACTACCAACCCTTTGGACCTGGCTGT GATGCGGCTGGCTGCCCTGGAACAGAATGTAGAACGGCGGTACCTGCGGGAGCCCCTCTGGCCAACTCAT GAGGTTGTGCTGGAGAAGGCCCTGCTTAGCACACCTAATGGTGCCCCTGAGGGCACCACTACAGAGATAT CATATGAGATCACCCCTCGCATTCGTGTCTGGCGCCAGACCCTCGAGCGGTGCCGGAGCGCAGCCCAGGT GTGCTTGTGCCTGGGCCAGCTGGAGAGGTCCATTGCCTGGGAGAAGTCTGTCAACAAAGTGACATGTCTA GTCTGCCGGAAGGGTGACAATGATGAGTTTCTTCTGCTTTGTGATGGGTGTGACCGTGGCTGCCACATTT ACTGCCATCGTCCCAAGATGGAGGCTGTCCCAGAAGGAGATTGGTTCTGTACTGTCTGTTTGGCTCAGCA GGTGGAGGGAGAATTCACTCAGAAGCCTGGTTTCCCAAAGCGTGGCCAGAAGCGGAAAAGTGGTTATTCG CTGAACTTCTCAGAGGGTGATGGCCGCCGACGCCGGGTACTGTTGAGGGGCCGAGAAAGCCCAGCAGCAG GGCCTCGGTACTCGGAAGAAGGGCTCTCCCCCTCCAAGCGGCGGCGACTCTCTATGCGGAACCACCACAG TGATCTCACATTTTGCGAGATTATCCTGATGGAGATGGAGTCCCATGATGCAGCCTGGCCTTTCCTAGAG CCTGTGAACCCACGTTTGGTGAGTGGGTACCGGCGCATCATCAAAAATCCTATGGATTTTTCCACCATGC GGGAGCGGCTGCTCAGGGGAGGGTACACCAGCTCAGAGGAGTTTGCGGCTGATGCCCTCCTGGTATTTGA CAACTGCCAGACTTTCAACGAGGATGACTCTGAAGTAGGCAAGGCTGGGCACATCATGCGCCGCTTCTTC GAGAGCCGCTGGGAGGAGTTTTATCAGGGAAAACAGGCCAATCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220374 representing NM_013449
Red=Cloning site Green=Tags(s) MEMEANDHFNFTGLPPAPAASGLKPSPSSGEGLYTNGSPMNFPQQGKSLNGDVNVNGLSTVSHTTTSGIL NSAPHSSSTSHLHHPSVAYDCLWNYSQYPSANPGSNLKDPPLLSQFSGGQYPLNGILGGSRQPSSPSHNT NLRAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNGPPSFFTSPQTSPMLGSSIQTFAPSQEVG SGIHPDEAAEKEMTSVVAENGTGLVGSLELEEEQPELKMCGYNGSVPSVESLHQEVSVLVPDPTVSCLDD PSHLPDQLEDTPILSEDSLEPFNSLAPEPVSGGLYGIDDTELMGAEDKLPLEDSPVISALDCPSLNNATA FSLLADDSQTSTSIFASPTSPPVLGESVLQDNSFDLNNGSDAEQEEMETQSSDFPPSLTQPAPDQSSTIQ LHPATSPAVSPTTSPAVSLVVSPAASPEISPEVCPAASTVVSPAVFSVVSPASSAVLPAVSLEVPLTASV TSPKASPVTSPAAAFPTASPANKDVSSFLETTADVEEITGEGLTASGSGDVMRRRIATPEEVRLPLQHGW RREVRIKKGSHRWQGETWYYGPCGKRMKQFPEVIKYLSRNVVHSVRREHFSFSPRMPVGDFFEERDTPEG LQWVQLSAEEIPSRIQAITGKRGRPRNTEKAKTKEVPKVKRGRGRPPKVKITELLNKTDNRPLKKLEAQE TLNEEDKAKIAKSKKKMRQKVQRGECQTTIQGQARNKRKQETKSLKQKEAKKKSKAEKEKGKTKQEKLKE KVKREKKEKVKMKEKEEVTKAKPACKADKTLATQRRLEERQRQQMILEEMKKPTEDMCLTDHQPLPDFSR VPGLTLPSGAFSDCLTIVEFLHSFGKVLGFDPAKDVPSLGVLQEGLLCQGDSLGEVQDLLVRLLKAALHD PGFPSYCQSLKILGEKVSEIPLTRDNVSEILRCFLMAYGVEPALCDRLRTQPFQAQPPQQKAAVLAFLVH ELNGSTLIINEIDKTLESMSSYRKNKWIVEGRLRRLKTVLAKRTGRSEVEMEGPEECLGRRRSSRIMEET SGMEEEEEEESIAAVPGRRGRRDGEVDATASSIPELERQIEKLSKRQLFFRKKLLHSSQMLRAVSLGQDR YRRRYWVLPYLAGIFVEGTEGNLVPEEVIKKETDSLKVAAHASLNPALFSMKMELAGSNTTASSPARARG RPRKTKPGSMQPRHLKSPVRGQDSEQPQAQLQPEAQLHAPAQPQPQLQLQLQSHKGFLEQEGSPLSLGQS QHDLSQSAFLSWLSQTQSHSSLLSSSVLTPDSSPGKLDPAPSQPPEEPEPDEAESSPDPQALWFNISAQM PCNAAPTPPPAVSEDQPTPSPQQLASSKPMNRPSAANPCSPVQFSSTPLAGLAPKRRAGDPGEMPQSPTG LGQPKRRGRPPSKFFKQMEQRYLTQLTAQPVPPEMCSGWWWIRDPEMLDAMLKALHPRGIREKALHKHLN KHRDFLQEVCLRPSADPIFEPRQLPAFQEGIMSWSPKEKTYETDLAVLQWVEELEQRVIMSDLQIRGWTC PSPDSTREDLAYCEHLSDSQEDITWRGRGREGLAPQRKTTNPLDLAVMRLAALEQNVERRYLREPLWPTH EVVLEKALLSTPNGAPEGTTTEISYEITPRIRVWRQTLERCRSAAQVCLCLGQLERSIAWEKSVNKVTCL VCRKGDNDEFLLLCDGCDRGCHIYCHRPKMEAVPEGDWFCTVCLAQQVEGEFTQKPGFPKRGQKRKSGYS LNFSEGDGRRRRVLLRGRESPAAGPRYSEEGLSPSKRRRLSMRNHHSDLTFCEIILMEMESHDAAWPFLE PVNPRLVSGYRRIIKNPMDFSTMRERLLRGGYTSSEEFAADALLVFDNCQTFNEDDSEVGKAGHIMRRFF ESRWEEFYQGKQANL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013449 |
| ORF Size | 5715 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013449.4 |
| RefSeq Size | 8934 bp |
| RefSeq ORF | 5718 bp |
| Locus ID | 11176 |
| UniProt ID | Q9UIF9 |
| Domains | AT_hook, BROMO, MBD, PHD, DDT |
| Protein Families | Druggable Genome |
| Gene Summary | Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).[UniProtKB/Swiss-Prot Function] |
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