United States
Germany
Japan
United Kingdom
China
PRDM8 (NM_020226) Human Tagged ORF Clone
CAT＃: RG220345
- TrueORF®
PRDM8 (tGFP-tagged) - Human PR domain containing 8 (PRDM8), transcript variant 1
ORF Plasmid: DDK
"NM_020226" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 9,672.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EPM10; KMT8D; PFM5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220345 representing NM_020226
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATACTGGCATCCAGCGAGGCATCTGGGATGGAGATGCCAAGGCTGTCCAACAATGTCTGACAG ATATTTTTACCAGCGTTTACACCACCTGCGACATCCCTGAGAATGCTATATTTGGTCCCTGTGTCCTGAG CCATACTTCCCTATATGACAGCATAGCTTTCATAGCTCTCAAGTCTACTGACAAGAGAACAGTACCGTAT ATCTTTCGGGTAGACACCTCAGCAGCAAATGGTTCCTCAGAAGGTCTCATGTGGCTGCGGTTGGTCCAAT CGGCCAGAGATAAGGAAGAGCAGAACCTTGAAGCCTACATAAAAAACGGACAGCTGTTCTACCGCTCTCT CCGCAGGATTGCCAAAGACGAGGAGTTACTAGTTTGGTACGGGAAAGAACTGACTGAGTTACTCTTGCTC TGCCCCTCTAGATCCCACAACAAAATGAATGGGTCGTCCCCTTACACATGCCTGGAATGCAGCCAACGTT TCCAGTTTGAGTTCCCCTATGTGGCGCATCTGCGTTTCCGCTGCCCCAAGAGACTTCACAGCGCTGATAT AAGTCCCCAAGACGAACAAGGCGGCGGCGTGGGCACCAAGGACCACGGGGGCGGCGGCGGCGGTGGCAAA GACCAGCAGCAGCAGCAGCAGGAGGCACCTTTAGGCCCGGGTCCCAAGTTTTGCAAAGCCGGCCCCCTCC ACCACTACCCATCCCCCTCCCCGGAAAGCAGCAACCCATCCGCTGCCGCCGGCGGCAGCAGCGCGAAGCC ATCCACAGACTTCCACAACCTGGCCAGGGAGCTGGAAAACTCCCGGGGAGGCAGCAGCTGCTCCCCAGCC CAGAGCCTCAGCAGCGGTAGCGGCAGCGGCGGCGGCGGCGGCCACCAGGAGGCGGAGCTGAGTCCCGACG GCATCGCCACGGGCGGCGGCAAAGGAAAGAGGAAATTCCCGGAGGAGGCGGCGGAGGGCGGCGGTGGCGC TGGTCTGGTAGGGGGCCGGGGCCGCTTCGTAGAGCGGCCCCTCCCGGCCTCCAAGGAGGATCTGGTGTGC ACACCGCAGCAGTACCGAGCCTCGGGCAGCTACTTCGGCCTGGAAGAGAACGGCCGCCTCTTCGCGCCGC CAAGTCCCGAGACGGGCGAGGCGAAGCGCAGCGCCTTCGTGGAGGTGAAGAAGGCTGCCCGCGCGGCCAG CCTGCAGGAGGAGGGGACAGCCGACGGCGCGGGAGTCGCCTCCGAGGACCAGGACGCTGGCGGCGGCGGC GGCTCCTCCACGCCCGCGGCCGCGTCACCGGTGGGCGCCGAGAAGCTGCTGGCCCCGCGGCCTGGGGGCC CGCTGCCCAGCCGGCTCGAGGGCGGCAGTCCTGCGAGGGGCAGCGCCTTCACTTCGGTGCCGCAGCTGGG CAGCGCGGGCAGCACCAGCGGTGGGGGCGGAACGGGCGCCGGGGCCGCAGGCGGCGCGGGCGGGGGCCAG GGCGCCGCGTCGGACGAGCGCAAAAGCGCCTTCTCGCAGCCAGCACGCTCTTTCTCGCAGCTGTCCCCGC TGGTGCTGGGCCAGAAGCTGGGCGCGCTCGAGCCATGCCACCCCGCCGACGGCGTGGGCCCCACCAGACT CTATCCCGCCGCCGCGGACCCTCTAGCGGTGAAGCTCCAGGGGGCCGCGGACCTGAACGGAGGTTGCGGG TCCCTGCCGAGCGGCGGCGGCGGCCTGCCTAAGCAGAGCCCCTTCCTGTACGCCACCGCCTTCTGGCCCA AGAGCTCCGCTGCCGCTGCAGCCGCGGCTGCGGCGGCGGCCGCGGGGCCCTTGCAGCTGCAGCTGCCCTC GGCGCTCACGCTGCTGCCGCCCTCCTTCACCTCGCTGTGTCTGCCCGCGCAGAACTGGTGCGCCAAGTGC AATGCCTCCTTCCGCATGACCTCCGACCTGGTGTACCATATGAGGTCGCACCACAAAAAGGAGTATGCGA TGGAGCCCTTGGTGAAGCGGCGGCGAGAGGAGAAACTCAAGTGCCCCATCTGCAATGAGTCCTTCAGGGA GCGCCACCACCTCTCCAGGCACATGACCTCGCATAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220345 representing NM_020226
Red=Cloning site Green=Tags(s) MEDTGIQRGIWDGDAKAVQQCLTDIFTSVYTTCDIPENAIFGPCVLSHTSLYDSIAFIALKSTDKRTVPY IFRVDTSAANGSSEGLMWLRLVQSARDKEEQNLEAYIKNGQLFYRSLRRIAKDEELLVWYGKELTELLLL CPSRSHNKMNGSSPYTCLECSQRFQFEFPYVAHLRFRCPKRLHSADISPQDEQGGGVGTKDHGGGGGGGK DQQQQQQEAPLGPGPKFCKAGPLHHYPSPSPESSNPSAAAGGSSAKPSTDFHNLARELENSRGGSSCSPA QSLSSGSGSGGGGGHQEAELSPDGIATGGGKGKRKFPEEAAEGGGGAGLVGGRGRFVERPLPASKEDLVC TPQQYRASGSYFGLEENGRLFAPPSPETGEAKRSAFVEVKKAARAASLQEEGTADGAGVASEDQDAGGGG GSSTPAAASPVGAEKLLAPRPGGPLPSRLEGGSPARGSAFTSVPQLGSAGSTSGGGGTGAGAAGGAGGGQ GAASDERKSAFSQPARSFSQLSPLVLGQKLGALEPCHPADGVGPTRLYPAAADPLAVKLQGAADLNGGCG SLPSGGGGLPKQSPFLYATAFWPKSSAAAAAAAAAAAAGPLQLQLPSALTLLPPSFTSLCLPAQNWCAKC NASFRMTSDLVYHMRSHHKKEYAMEPLVKRRREEKLKCPICNESFRERHHLSRHMTSHN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020226 |
| ORF Size | 2067 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020226.3, NP_064611.3 |
| RefSeq Size | 4118 bp |
| RefSeq ORF | 2070 bp |
| Locus ID | 56978 |
| UniProt ID | Q9NQV8 |
| Domains | SET |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a protein that belongs to a conserved family of histone methyltransferases that predominantly act as negative regulators of transcription. The encoded protein contains an N-terminal Su(var)3-9, Enhancer-of-zeste, and Trithorax (SET) domain and a double zinc-finger domain. Knockout of this gene in mouse results in mistargeting by neurons of the dorsal telencephalon, abnormal itch-like behavior, and impaired differentiation of rod bipolar cells. In humans, the protein has been shown to interact with the phosphatase laforin and the ubiquitin ligase malin, which regulate glycogen construction in the cytoplasm. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220345 | PRDM8 (Myc-DDK-tagged)-Human PR domain containing 8 (PRDM8), transcript variant 1 |
CNY 8,072.00 |
|
| RC220345L3 | Lenti-ORF clone of PRDM8 (Myc-DDK-tagged)-Human PR domain containing 8 (PRDM8), transcript variant 1 |
CNY 7,700.00 |
|
| RC220345L4 | Lenti-ORF clone of PRDM8 (mGFP-tagged)-Human PR domain containing 8 (PRDM8), transcript variant 1 |
CNY 7,700.00 |
|
| SC108651 | PRDM8 (untagged)-Human PR domain containing 8 (PRDM8), transcript variant 1 |
CNY 3,600.00 |
|
| SC318162 | PRDM8 (untagged)-Human PR domain containing 8 (PRDM8), transcript variant 1 |
CNY 7,220.00 |
