AKAP7 (NM_016377) Human Tagged ORF Clone

CAT#: RG219968

  • TrueORF®

AKAP7 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 7 (AKAP7), transcript variant gamma

ORF Plasmid: DDK tGFP



  "NM_016377" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


AKAP7 mouse monoclonal antibody, clone OTI6F7 (formerly 6F7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms AKAP15; AKAP18
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG219968 representing NM_016377
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGAGGAATTTGAAGCCAATACTATGGATTCTCTGGTAGACATGCCATTTGCTACTGTAGATATTC
AGGATGACTGTGGAATCACTGATGAACCTCAAATAAATTTGAAGAGAAGTCAAGAAAATGAATGGGTCAA
GAGTGATCAAGTAAAGAAGAGGAAAAAAAAGAGAAAAGATTATCAACCCAACTATTTCCTGTCCATTCCA
ATCACCAACAAAGAGATTATAAAAGGAATTAAGATCCTGCAGAATGCAATAATACAACAAGATGAGCGAC
TGGCCAAAGCAATGGTCAGTGATGGTTCCTTTCATATTACCCTGCTGGTGATGCAATTATTAAATGAAGA
TGAAGTAAACATTGGTATTGATGCTCTTTTGGAATTGAAACCATTCATAGAAGAACTCCTCCAGGGAAAA
CATTTGACTTTGCCCTTTCAAGGGATTGGTACTTTTGGAAATCAGGTTGGATTTGTGAAGCTGGCAGAAG
GAGATCATGTAAACTCACTTTTGGAGATAGCAGAGACTGCAAATAGGACATTTCAAGAAAAAGGCATCCT
GGTAGGAGAGAGCAGAAGTTTTAAACCTCATTTGACCTTCATGAAGTTGTCAAAATCACCGTGGCTCCGT
AAGAATGGAGTGAAAAAAATAGATCCTGATTTATATGAAAAGTTTATCAGTCACAGATTTGGAGAAGAAA
TATTATATCGCATAGATCTTTGCTCCATGCTGAAGAAAAAACAAAGTAATGGTTATTATCACTGTGAATC
TTCCATTGTGATTGGTGAAAAGAACGGAGGGGAGCCCGATGACGCTGAACTAGTAAGGCTCAGTAAGAGG
CTGGTGGAGAACGCGGTGCTCAAGGCTGTCCAGCAGTATCTGGAGGAAACACAGAATAAAAACAAGCCGG
GGGAGGGGAGCTCTGTGAAAACCGAAGCAGCTGATCAGAATGGCAATGACAATGAGAACAACAGGAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG219968 representing NM_016377
Red=Cloning site Green=Tags(s)

MSEEFEANTMDSLVDMPFATVDIQDDCGITDEPQINLKRSQENEWVKSDQVKKRKKKRKDYQPNYFLSIP
ITNKEIIKGIKILQNAIIQQDERLAKAMVSDGSFHITLLVMQLLNEDEVNIGIDALLELKPFIEELLQGK
HLTLPFQGIGTFGNQVGFVKLAEGDHVNSLLEIAETANRTFQEKGILVGESRSFKPHLTFMKLSKSPWLR
KNGVKKIDPDLYEKFISHRFGEEILYRIDLCSMLKKKQSNGYYHCESSIVIGEKNGGEPDDAELVRLSKR
LVENAVLKAVQQYLEETQNKNKPGEGSSVKTEAADQNGNDNENNRK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016377
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016377.2, NP_057461.1
RefSeq Size 2821 bp
RefSeq ORF 1047 bp
Locus ID 9465
UniProt ID Q9P0M2
Protein Families Druggable Genome
Gene Summary This gene encodes a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Apr 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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