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PABPC4 (NM_003819) Human Tagged ORF Clone
CAT＃: RG219691
- TrueORF®
PABPC4 (tGFP-tagged) - Human poly(A) binding protein, cytoplasmic 4 (inducible form) (PABPC4), transcript variant 2
ORF Plasmid: DDK
"NM_003819" in other vectors (4)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | APP-1; APP1; iPABP; PABP4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG219691 representing NM_003819
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGCTGCGGCCAGCAGCTACCCCATGGCCTCCCTGTACGTGGGCGACCTGCATTCGGACGTCACCG AGGCCATGCTGTACGAAAAGTTCAGCCCCGCGGGGCCTGTGCTGTCCATCCGGGTCTGCCGCGATATGAT CACCCGCCGCTCCCTGGGCTATGCCTACGTCAACTTCCAGCAGCCGGCCGACGCTGAGCGGGCTTTGGAC ACCATGAACTTTGATGTGATTAAGGGAAAGCCAATCCGCATCATGTGGTCTCAGAGGGATCCCTCTTTGA GAAAATCTGGTGTGGGAAACGTCTTCATCAAGAACCTGGACAAATCTATAGATAACAAGGCACTTTATGA TACTTTTTCTGCTTTTGGAAACATACTGTCCTGCAAGGTGGTGTGTGATGAGAACGGCTCTAAGGGTTAT GCCTTTGTCCACTTCGAGACCCAAGAGGCTGCCGACAAGGCCATCGAGAAGATGAATGGCATGCTCCTCA ATGACCGCAAAGTATTTGTGGGCAGATTCAAGTCTCGCAAAGAGCGGGAAGCTGAGCTTGGAGCCAAAGC CAAGGAATTCACCAATGTTTATATCAAAAACTTTGGGGAAGAGGTGGATGATGAGAGTCTGAAAGAGCTA TTCAGTCAGTTTGGTAAGACCCTAAGTGTCAAGGTGATGAGAGATCCCAATGGGAAATCCAAAGGCTTTG GCTTTGTGAGTTACGAAAAACACGAGGATGCCAATAAGGCTGTGGAAGAGATGAATGGAAAAGAAATAAG TGGTAAAATCATATTTGTAGGCCGTGCACAAAAGAAAGTAGAACGGCAGGCAGAGTTAAAACGGAAATTT GAACAGTTGAAACAGGAGAGAATTAGTCGATATCAGGGGGTGAATCTCTACATTAAGAACTTGGATGACA CTATTGATGATGAGAAATTAAGGAAAGAATTTTCTCCTTTTGGATCAATTACCAGTGCTAAGGTAATGCT GGAGGATGGAAGAAGCAAAGGGTTTGGCTTCGTCTGCTTCTCATCTCCTGAAGAAGCAACCAAAGCAGTC ACTGAGATGAATGGACGCATTGTGGGCTCCAAGCCACTATATGTTGCCCTGGCCCAGAGGAAGGAAGAGA GAAAGGCTCACCTGACCAACCAGTATATGCAACGAGTGGCTGGAATGAGAGCACTTCCTGCCAATGCCAT CTTAAATCAGTTCCAGCCTGCAGCGGGTGGCTACTTTGTGCCAGCAGTCCCACAGGCTCAGGGAAGGCCT CCATATTATACACCTAACCAGTTAGCACAGATGAGGCCTAATCCACGCTGGCAGCAAGGTGGGAGACCTC AAGGCTTCCAAGGAATGCCAAGTGCTATACGCCAGTCTGGGCCTCGTCCAACTCTTCGCCATCTGGCTCC AACTGGGTCTGAGTGCCCGGACCGCTTGGCTATGGACTTTGGTGGGGCTGGTGCCGCCCAGCAAGGGCTG ACTGACAGCTGCCAGTCTGGAGGCGTTCCCACAGCTGTGCAGAACTTAGCGCCACGCGCTGCTGTTGCTG CTGCTGCTCCCCGGGCTGTTGCCCCCTACAAATACGCCTCCAGTGTCCGCAGCCCTCATCCTGCCATACA GCCTCTGCAGGCACCCCAGCCTGCGGTCCATGTGCAGGGGCAGGAGCCACTGACTGCCTCCATGCTGGCT GCAGCACCCCCCCAGGAACAGAAGCAGATGCTGGGAGAACGCTTGTTCCCACTCATCCAAACAATGCATT CAAATCTGGCTGGGAAGATCACGGGAATGCTGCTGGAGATAGACAACTCTGAGCTGCTGCACATGTTAGA GTCCCCCGAGTCTCTCCGCTCCAAGGTGGATGAAGCTGTAGCAGTTCTACAGGCTCATCATGCCAAGAAA GAAGCTGCCCAGAAGGTGGGCGCTGTTGCTGCTGCTACCTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219691 representing NM_003819
Red=Cloning site Green=Tags(s) MNAAASSYPMASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALD TMNFDVIKGKPIRIMWSQRDPSLRKSGVGNVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGY AFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDESLKEL FSQFGKTLSVKVMRDPNGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKKVERQAELKRKF EQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEDGRSKGFGFVCFSSPEEATKAV TEMNGRIVGSKPLYVALAQRKEERKAHLTNQYMQRVAGMRALPANAILNQFQPAAGGYFVPAVPQAQGRP PYYTPNQLAQMRPNPRWQQGGRPQGFQGMPSAIRQSGPRPTLRHLAPTGSECPDRLAMDFGGAGAAQQGL TDSCQSGGVPTAVQNLAPRAAVAAAAPRAVAPYKYASSVRSPHPAIQPLQAPQPAVHVQGQEPLTASMLA AAPPQEQKQMLGERLFPLIQTMHSNLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHHAKK EAAQKVGAVAAATS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003819 |
| ORF Size | 1932 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003819.4 |
| RefSeq Size | 3182 bp |
| RefSeq ORF | 1935 bp |
| Locus ID | 8761 |
| UniProt ID | Q13310 |
| Domains | RRM, PABP, RRM_1 |
| Gene Summary | Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219691 | PABPC4 (Myc-DDK-tagged)-Human poly(A) binding protein, cytoplasmic 4 (inducible form) (PABPC4), transcript variant 2 |
CNY 5,700.00 |
|
| RC219691L3 | Lenti-ORF clone of PABPC4 (Myc-DDK-tagged)-Human poly(A) binding protein, cytoplasmic 4 (inducible form) (PABPC4), transcript variant 2 |
CNY 7,600.00 |
|
| RC219691L4 | Lenti-ORF clone of PABPC4 (mGFP-tagged)-Human poly(A) binding protein, cytoplasmic 4 (inducible form) (PABPC4), transcript variant 2 |
CNY 7,600.00 |
|
| SC117732 | PABPC4 (untagged)-Human poly(A) binding protein, cytoplasmic 4 (inducible form) (PABPC4), transcript variant 2 |
CNY 4,800.00 |
