TMEM185B (NR_000034) Human Tagged ORF Clone
CAT#: RG219480
- TrueORF®
TMEM185B (tGFP-tagged) - Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA
ORF Plasmid: DDK
"NR_000034" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | FAM11B; FLJ20979 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG219480 representing NR_000034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCCCAGGGGCCTGTTCCAGGACTTCAACCCCAGTAAGTTTCTCATCTACACCTGCCTGCTGCTCT TCTCGGTGCTGCTGCCCCTCCGCCTGGACGGCATCATCCAATGGAGCTACTGGGCCGTCTTTGCCCCCAT ATGGCTGTGGAAGCTTCTAGTCGTCGCAGGCGCCTCCGTGGGCGCGGGCGTTTGGGCCCGCAACCCTCGC TACCGCACCGAGGGAGAGGCCTGTGTGGAGTTCAAAGCCATGCTGATCGCTGTGGGCATCCACCTGCTGC TGCTCATGTTCGAAGTCCTGGTCTGCGACAGGGTGGAGAGGGGCACCCACTTCTGGCTGCTGGTCTTCAT GCCTCTCTTCTTCGTGTCCCCCGTGTCCGTGGCTGCCTGCGTCTGGGGCTTTCGACACGATAGGTCGCTG GAGCTGGAGATCCTGTGCTCGGTCAACATCCTGCAGTTCATCTTCATCGCCCTAAAGCTGGACAGGATTA TTCACTGGCCGTGGCTGGTGGTGTTTGTGCCCCTGTGGATCCTCATGTCGTTCCTTTGCCTGGTCGTCCT CTATTACATCGTCTGGTCCCTCCTGTTCCTGCGGTCCCTGGATGTGGTTGCCGAGCAGCGGAGAACACAC GTGACCATGGCTATCAGTTGGATAACGATTGTCGTGCCTCTGCTCACTTTTGAGGTCCTGCTGGTTCACA GATTGGATGGCCACAATACATTCTCCTACGTCTCCATATTTGTCCCCCTTTGGCTTTCCTTACTAACTTT AATGGCCACAACATTTAGGCGAAAGGGGGGCAATCATTGGTGGTTTGGCATTCGCAGAGACTTCTGTCAG TTTCTGCTTGAAATTTTCCCATTTTTAAGAGAATATGGGAACATTTCATATGATCTCCATCACGAAGATA GTGAAGATGCTGAAGAAACATCAGTTCCAGAAGCTCCGAAAATTGCTCCAATATTTGGAAAGAAGGCCAG AGTAGTTATAACCCAGAGCCCTGGGAAATACGTTCCCCCCCCTCCCAAGTTAAATATTGATATGCCAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219480 representing NR_000034
Red=Cloning site Green=Tags(s) MNPRGLFQDFNPSKFLIYTCLLLFSVLLPLRLDGIIQWSYWAVFAPIWLWKLLVVAGASVGAGVWARNPR YRTEGEACVEFKAMLIAVGIHLLLLMFEVLVCDRVERGTHFWLLVFMPLFFVSPVSVAACVWGFRHDRSL ELEILCSVNILQFIFIALKLDRIIHWPWLVVFVPLWILMSFLCLVVLYYIVWSLLFLRSLDVVAEQRRTH VTMAISWITIVVPLLTFEVLLVHRLDGHNTFSYVSIFVPLWLSLLTLMATTFRRKGGNHWWFGIRRDFCQ FLLEIFPFLREYGNISYDLHHEDSEDAEETSVPEAPKIAPIFGKKARVVITQSPGKYVPPPPKLNIDMPD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NR_000034 |
ORF Size | 657 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NR_000034.1 |
RefSeq Size | 2131 bp |
RefSeq ORF | 1049 bp |
Locus ID | 79134 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219480 | TMEM185B (Myc-DDK-tagged)-Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA |
CNY 3,656.00 |
|
RC219480L3 | Lenti ORF clone of Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC219480L4 | Lenti ORF clone of Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA, mGFP tagged |
CNY 5,890.00 |
|
SC126776 | TMEM185B (untagged)-Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA |
CNY 2,400.00 |
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