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DENND4C (NM_017925) Human Tagged ORF Clone
CAT＃: RG219039
- TrueORF®
DENND4C (tGFP-tagged) - Human DENN/MADD domain containing 4C (DENND4C)
ORF Plasmid: DDK
"NM_017925" in other vectors (2)
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CNY 18,464.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bA513M16.3; C9orf55; C9orf55B; RAB10GEF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG219039 representing NM_017925
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCTACTATTGAGTGCTGGGATCCTGAAACCAAATATCCACTTCCAGTTTTTTCAACTTTTGTCT TGACAGGTTCTTCAGCCAAAAAGGTATATGGAGCTGCCATTCAGTTTTATGAACCTTACTCTCGGGAACT TCTATCAGAGAAACAGCTTATGCACCTGGGCTTGTTGACGCCTGTGGAGAGAAAAATGGTCTCCAAATCC ATCAATACAAACAAATGCATTTGTTTACTCTCACACTGGCCTTTTTTTGAAGCTTTTAGGAAATTTCTTA TGTTTATCTACAAACTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACATTTTAT GCAAAACATCCCTTTTCCTTCACCACAAAGACCGAGAATCCTTGTCCAGCTGTCAGTCCATGATGCATTA ATATTATCACAGCCAGTTTCTACACCTTTACCACTAAGTGGAGCCAACTTTAGCACCTTGCTAATGAATC TGGGTCCTGAGAATTGTGCAACACTGCTGCTCTTTGTTTTACTTGAGAGTAAAATTCTGCTGCATTCTCT TAGGCCAGCTGTCTTGACTGGGGTAGCTGAAGCTGTTGTAGCTATGATCTTTCCATTTCAGTGGCAATGC CCATATATTCCCCTTTGTCCTCTTTCACTGGCTGCAGTGCTTAGTGCACCTTTACCATTTATAGTTGGAG TTGACTCAAGGTATTTTGATCTTCATGACCCACCACAAGATGTTGTTTGCATTGACTTGGATACGAACAT GTTATATGTATCAGATGAAAAGAAGAACATGAACTGGAAGCAACTTCCCAAAAAGCCGTGCAAAAATCTA CTTAGCACCTTAAAGAAATTGTATCCCCAGCTGTCTTCAGTTCACCAAAAAACTCAAGAAGGCTCAGCGA TTGACATGACTCCAATTGAAGCAGATTTCTCCTGGCAAAAGAAGATGACACAGCTTGAGATGGAAATTCA AGAGGCATTTTTGCGCTTTATGGCGTCTATTTTAAAAGGATATAGAACATATCTCAGACCAATCACAGAG GCTCCTTCAAATAAAGCCACAGCTGCTGATTCATTGTTTGACCGACAGGGATTTTTAAAAAGTCGAGATC GTGCCTATGCAAAATTCTATACCCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAATGCAG TTTTGTAAGTGATAAAGATACTGGATTAGCATTTTTTGATGACTGCATAGAAAAGTTGTTTCCTGATAAA GGCACAGAGAAAACAGATAAGGTTGATTTTGATTCAGCAGAAGATACCAGATTGATAGAACTAGATGATT CACAGAAAAGTGAGCATACTGTATTTATAATGCCGCCAGAGCCACCTCCTGATGATGGAAAGGACCTGTC ACCAAAGTACAGTTACAAATACTTTCCAAGACTGGACCTTAAGCTTTTTGACAGACCGCAGGAGTTGAAA CTTTGTTTTAGTAGACACCCTACTGGGAATAGCATTACAAAGAGTCCACCTCTCATGGCTAAGAGAACTA AACAGGAAATAAAAACAGCTCATAAATTGGCGAAGAGATGTTATACAAATCCACCACAGTGGGCCAAGTG TCTGTTTAGTCATTGTTACAGTTTATGGTTTATTTGTCTTCCGGCCTATGTTAGAGTTTCTCATCCTAAA GTCAGAGCACTTCAGCAGGCATATGATGTACTTATTAAGATGAGGAAAACAGATGTGGATCCCTTAGATG AGGTGTGCTATCGAGTAGTGATGCAGCTTTGTGGACTTTGGGGTCATCCTGTTTTAGCAGTGAGAGTCTT ATTTGAAATGAAAACTGCTAGGATAAAGCCTAATGCTATTACTTATGGTTATTATAATAAGGTAGTCTTG GAGAGCCCGTGGCCTAGCAGTACCCGCAGTGGTATTTTCTTATGGACGAAGGTACGGAATGTGGTACGTG GCTTGGCACAGTTTAGGCAGCCGCTTAAAAAGACTGTGCAAAGGTCACAGGTCTCCTCAATATCAGGTGG TCAGTCTGACCAAGGATACGGGTCTAAGGATGAACTTATAAAGGATGATGCAGAAATTCATGTGCCTGAA GAACAGGCAGCAAGAGAATTGATAACTAAAACAAAAATGCAAACAGAAGAGGTGTGTGATGCCTCTGCTA TTGTGGCAAAACATTCACAACCTAGTCCAGAGCCTCACAGTCCTACTGAACCTCCTGCATGGGGCAGCAG TATTGTGAAAGTTCCGTCTGGTATATTTGATGTCAACAGCAGGAAAAGTAGCACTGGTAGTATATCAAAT GTGCTGTTTTCTACTCAAGATCCAGTTGAAGATGCAGTCTTTGGCGAAGCTACTAATCTCAAGAAGAATG GTGATAGAGGAGAAAAAAGACAAAAGCATTTTCCTGAGAGGAGTTGTAGTTTTAGTTCTGAAAGTCGAGC AGGAATGTTGCTTAAGAAGAGTAGTTTGGATTCGAATTCAAGTGAAATGGCTATCATGATGGGAGCAGAT GCCAAGATTCTCACAGCAGCATTGACATGTCCTAAGACTTCTCTACTTCATATTGCAAGAACCCATAGCT TTGAGAATGTTAGCTGTCACCTACCTGATAGTAGGACTTGTATGTCTGAAAGCACTTGGAATCCTGAGCA CAGATCATCTCCGGTGCCAGAGATGCTTGAGGAAAGCCAAGAACTCCTTGAGCCTGTGGTTGATGACGTA CCTAAAACTACTGCAACAGTAGATACATATGAGAGTCTACTAAGTGATAGTAACAGTAATCAGTCCAGAG ACTTGAAAACAGTATCCAAAGATCTGAGGAATAAGAGAAGTAGTTTATATGGTATTGCTAAGGTGGTTCA GAGGGAAGATGTTGAAACTGGACTAGATCCTTTGTCTCTTTTAGCCACTGAATGTACAGGAGGAAAAACT CCTGATTCTGAAGATAAGTTGTTTTCTCCAGTTATTGCACGTAATCTGGCTGATGAAATAGAAAGCTATA TGAACCTAAAAAGTCCCCTAGGTAGTAAATCTTCTAGTATGGAATTACACAGAGAGGAAAACAGAGAGTC TGGCATGACTACTGCATTTATTCATGCTCTAGAGAGGAGATCAAGCCTACCTTTAGATCATGGTTCACCA GCACAGGAAAATCCTGAAAGTGAAAAGAGCTCACCTGCAGTGTCCAGGTCTAAAACTTTTACTGGGCGTT TCAAGCAGCAAACCCCCTCTCGAACTCATAAAGAACGTTCAACTTCTTTGTCAGCACTGGTGCGTTCTTC GCCACATGGCTCGTTGGGTTCTGTAGTAAATTCTTTGTCAGGGCTAAAGCTGGATAATATACTCTCAGGG CCCAAGATAGATGTCCTGAAATCTGGTATGAAACAAGCAGCGACAGTAGCCAGTAAGATGTGGGTAGCTG TTGCGTCTGCCTACAGCTACTCAGATGATGAGGAAGAAACTAATAGAGACTACAGCTTCCCAGCTGGCCT AGAAGACCATATTTTGGGGGAGAATATATCGCCTAACACAAGTATCTCAGGGTTGGTCCCCAGTGAACTT ACCCAGAGCAACACAAGTCTTGGCAGTAGCAGCAGTAGTGGAGATGTAGGAAAACTGCATTATCCAACAG GTGAAGTTCCATTTCCAAGAGGCATGAAAGGGCAAGACTTTGAAAAATCAGATCATGGTTCTTCTCAAAA TACCAGCATGTCTAGCATCTATCAGAATTGTGCAATGGAGGTTTTGATGTCCAGTTGTTCACAGTGTAGA GCTTGTGGAGCTTTAGTTTATGATGAAGAAATTATGGCTGGATGGACAGCAGATGACTCAAATTTGAATA CAGCTTGTCCATTCTGTAAAAGCAACTTCTTGCCTCTTCTCAATATAGAATTCAAAGATTTGAGAGGTTC TGCAAGCTTTTTCCTGAAACCAAGTACCTCTGGTGACAGTTTACAAAGTGGAAGCATTCCATTGGCAAAT GAATCCTTGGAGCACAAACCTGTATCCAGTTTAGCAGAACCTGACTTGATCAACTTTATGGACTTCCCAA AACATAACCAGATCATAACTGAAGAAACAGGCTCTGCAGTTGAACCAAGTGATGAAATAAAGAGAGCCAG TGGAGATGTCCAAACTATGAAAATTTCATCTGTGCCTAATAGTTTATCAAAGCGAAATGTGTCTTTGACT CGAAGTCACAGTGTTGGAGGCCCATTGCAGAATATTGACTTTACCCAGCGACCGTTTCATGGCATCTCAA CAGTTAGTCTTCCAAATAGTCTGCAGGAAGTTGTGGATCCTTTAGGAAAAAGACCCAATCCTCCCCCTGT TTCTGTGCCCTACTTGAGTCCTCTAGTACTCCGTAAAGAACTTGAATCTTTGCTAGAAAATGAAGGTGAT CAGGTGATTCATACATCTTCTTTCATCAATCAACATCCAATCATTTTCTGGAACCTCGTTTGGTATTTCA GACGTTTGGACCTTCCTAGTAACTTGCCAGGACTTATCCTCACATCTGAACATTGTAATGAAGGTGTACA GCTTCCTCTGTCATCTCTGTCCCAGGATAGCAAACTTGTGTATATTCAGCTGTTATGGGATAATATCAAC CTTCATCAGGAACCAAGAGAACCTCTGTATGTCTCATGGAGGAATTTTAATTCTGAAAAGAAATCATCTC TCCTGTCAGAGGAACAACAAGAAACAAGCACTTTAGTAGAAACCATCAGGCAGAGTATTCAGCACAATAA TGTTCTTAAACCCATCAACCTACTTTCACAGCAAATGAAGCCAGGCATGAAAAGACAAAGGAGTTTATAC AGAGAAATCCTCTTCTTATCATTAGTGTCTCTAGGAAGAGAGAATATTGATATTGAGGCATTTGACAATG AATATGGAATTGCATACAATAGTCTGTCTTCAGAGATTCTTGAAAGGTTGCAGAAAATTGATGCTCCACC AAGTGCCAGTGTCGAGTGGTGCAGGAAGTGTTTTGGAGCGCCTCTCATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219039 representing NM_017925
Red=Cloning site Green=Tags(s) MGATIECWDPETKYPLPVFSTFVLTGSSAKKVYGAAIQFYEPYSRELLSEKQLMHLGLLTPVERKMVSKS INTNKCICLLSHWPFFEAFRKFLMFIYKLSVSGPHPLPIEKHISHFMQNIPFPSPQRPRILVQLSVHDAL ILSQPVSTPLPLSGANFSTLLMNLGPENCATLLLFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQC PYIPLCPLSLAAVLSAPLPFIVGVDSRYFDLHDPPQDVVCIDLDTNMLYVSDEKKNMNWKQLPKKPCKNL LSTLKKLYPQLSSVHQKTQEGSAIDMTPIEADFSWQKKMTQLEMEIQEAFLRFMASILKGYRTYLRPITE APSNKATAADSLFDRQGFLKSRDRAYAKFYTLLSKTQIFIRFIEECSFVSDKDTGLAFFDDCIEKLFPDK GTEKTDKVDFDSAEDTRLIELDDSQKSEHTVFIMPPEPPPDDGKDLSPKYSYKYFPRLDLKLFDRPQELK LCFSRHPTGNSITKSPPLMAKRTKQEIKTAHKLAKRCYTNPPQWAKCLFSHCYSLWFICLPAYVRVSHPK VRALQQAYDVLIKMRKTDVDPLDEVCYRVVMQLCGLWGHPVLAVRVLFEMKTARIKPNAITYGYYNKVVL ESPWPSSTRSGIFLWTKVRNVVRGLAQFRQPLKKTVQRSQVSSISGGQSDQGYGSKDELIKDDAEIHVPE EQAARELITKTKMQTEEVCDASAIVAKHSQPSPEPHSPTEPPAWGSSIVKVPSGIFDVNSRKSSTGSISN VLFSTQDPVEDAVFGEATNLKKNGDRGEKRQKHFPERSCSFSSESRAGMLLKKSSLDSNSSEMAIMMGAD AKILTAALTCPKTSLLHIARTHSFENVSCHLPDSRTCMSESTWNPEHRSSPVPEMLEESQELLEPVVDDV PKTTATVDTYESLLSDSNSNQSRDLKTVSKDLRNKRSSLYGIAKVVQREDVETGLDPLSLLATECTGGKT PDSEDKLFSPVIARNLADEIESYMNLKSPLGSKSSSMELHREENRESGMTTAFIHALERRSSLPLDHGSP AQENPESEKSSPAVSRSKTFTGRFKQQTPSRTHKERSTSLSALVRSSPHGSLGSVVNSLSGLKLDNILSG PKIDVLKSGMKQAATVASKMWVAVASAYSYSDDEEETNRDYSFPAGLEDHILGENISPNTSISGLVPSEL TQSNTSLGSSSSSGDVGKLHYPTGEVPFPRGMKGQDFEKSDHGSSQNTSMSSIYQNCAMEVLMSSCSQCR ACGALVYDEEIMAGWTADDSNLNTACPFCKSNFLPLLNIEFKDLRGSASFFLKPSTSGDSLQSGSIPLAN ESLEHKPVSSLAEPDLINFMDFPKHNQIITEETGSAVEPSDEIKRASGDVQTMKISSVPNSLSKRNVSLT RSHSVGGPLQNIDFTQRPFHGISTVSLPNSLQEVVDPLGKRPNPPPVSVPYLSPLVLRKELESLLENEGD QVIHTSSFINQHPIIFWNLVWYFRRLDLPSNLPGLILTSEHCNEGVQLPLSSLSQDSKLVYIQLLWDNIN LHQEPREPLYVSWRNFNSEKKSSLLSEEQQETSTLVETIRQSIQHNNVLKPINLLSQQMKPGMKRQRSLY REILFLSLVSLGRENIDIEAFDNEYGIAYNSLSSEILERLQKIDAPPSASVEWCRKCFGAPLI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017925 |
| ORF Size | 5019 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017925.4, NP_060395.4 |
| RefSeq Size | 7023 bp |
| RefSeq ORF | 5730 bp |
| Locus ID | 55667 |
| UniProt ID | Q5VZ89 |
| Gene Summary | Guanine nucleotide exchange factor (GEF) activating RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB10 into its active GTP-bound form. Thereby, stimulates SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in response to insulin.[UniProtKB/Swiss-Prot Function] |
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