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KRT35 (NM_002280) Human Tagged ORF Clone
CAT＃: RG218691
- TrueORF®
KRT35 (tGFP-tagged) - Human keratin 35 (KRT35)
ORF Plasmid: DDK
"NM_002280" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ha-5; HA5; hHa5; K35; KRTHA5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218691 representing NM_002280
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCAAATGCCTCAAGGCCGGCTTCTCTTCTGGGTCTCTCAAGAGCCCAGGAGGGGCCAGTGGGG GCTCCACTCGTGTGTCCGCAATGTACTCCAGCAGCTCTTGCAAGCTTCCAAGTCTCTCCCCTGTGGCCAG AAGTTTCTCTGCCTGCTCAGTGGGTCTGGGCAGAAGCAGCTACAGGGCCACCAGCTGCCTCCCTGCTCTC TGCCTCCCTGCTGGAGGCTTCGCTACCAGCTACAGTGGGGGTGGGGGCTGGTTTGGGGAGGGCATCCTCA CTGGCAATGAGAAGGAGACCATGCAATCCCTGAACGACCGCCTGGCCGGCTACCTGGAGAAGGTGCGTCA GCTGGAGCAGGAGAACGCCAGCCTGGAGAGCCGCATCCGTGAGTGGTGTGAGCAGCAGGTCCCCTACATG TGCCCTGACTACCAGTCCTACTTCCGGACCATCGAGGAGCTCCAGAAGAAGACTCTATGCAGCAAGGCTG AGAATGCCAGGCTGGTGGTGGAGATTGACAATGCCAAATTGGCTGCAGATGACTTCAGGACCAAGTATGA GACGGAGGTGTCCCTGCGGCAGCTGGTGGAGTCAGACATCAACGGCCTGCGCAGGATCCTGGATGACCTG ACCCTGTGCAAGTCTGACCTGGAGGCCCAGGTGGAGTCCCTGAAGGAGGAGCTGCTCTGCCTGAAGAAGA ACCATGAGGAGGAAGTGAACTCACTGCGCTGCCAACTTGGTGACCGCCTCAATGTTGAGGTGGACGCTGC CCCACCTGTTGACCTGAACCGAGTTCTGGAGGAGATGAGGTGCCAGTATGAAACCCTGGTGGAGAATAAC CGCCGGGATGCTGAAGACTGGTTGGACACCCAGAGTGAGGAGCTGAACCAGCAGGTGGTGTCCAGCTCAG AGCAGTTGCAGTCCTGCCAGGCAGAGATCATCGAGCTGAGACGCACGGTCAACGCCCTGGAGATTGAGCT GCAGGCTCAGCACAGCATGAGAGATGCTTTGGAATCCACCCTGGCAGAGACGGAGGCCCGCTATAGCTCC CAGCTGGCCCAGATGCAGTGCATGATCACCAACGTGGAGGCCCAGCTGGCCGAGATCCGGGCTGACCTGG AGCGGCAGAACCAGGAGTACCAGGTGCTGCTGGACGTCCGGGCCCGGCTGGAGTGTGAGATCAACACGTA CCGGGGCCTGCTGGAGAGTGAGGACAGCAAGCTCCCCTGTAACCCATGTGCACCTGACTACTCACCCTCC AAGTCATGCCTTCCCTGTCTTCCTGCGGCCTCCTGCGGTCCTAGTGCAGCCCGCACAAACTGCAGCCCCC GCCCCATTTGTGTGCCCTGCCCAGGGGGTCGGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218691 representing NM_002280
Red=Cloning site Green=Tags(s) MASKCLKAGFSSGSLKSPGGASGGSTRVSAMYSSSSCKLPSLSPVARSFSACSVGLGRSSYRATSCLPAL CLPAGGFATSYSGGGGWFGEGILTGNEKETMQSLNDRLAGYLEKVRQLEQENASLESRIREWCEQQVPYM CPDYQSYFRTIEELQKKTLCSKAENARLVVEIDNAKLAADDFRTKYETEVSLRQLVESDINGLRRILDDL TLCKSDLEAQVESLKEELLCLKKNHEEEVNSLRCQLGDRLNVEVDAAPPVDLNRVLEEMRCQYETLVENN RRDAEDWLDTQSEELNQQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHSMRDALESTLAETEARYSS QLAQMQCMITNVEAQLAEIRADLERQNQEYQVLLDVRARLECEINTYRGLLESEDSKLPCNPCAPDYSPS KSCLPCLPAASCGPSAARTNCSPRPICVPCPGGRF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002280 |
| ORF Size | 1365 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002280.4, NP_002271.3 |
| RefSeq Size | 1688 bp |
| RefSeq ORF | 1368 bp |
| Locus ID | 3886 |
| UniProt ID | Q92764 |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218691 | KRT35 (Myc-DDK-tagged)-Human keratin 35 (KRT35) |
CNY 3,656.00 |
|
| RC218691L3 | Lenti ORF clone of Human keratin 35 (KRT35), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218691L4 | Lenti ORF clone of Human keratin 35 (KRT35), mGFP tagged |
CNY 5,890.00 |
|
| SC317748 | KRT35 (untagged)-Human keratin 35 (KRT35) |
CNY 7,220.00 |
