United States
Germany
Japan
United Kingdom
China
KRT74 (NM_175053) Human Tagged ORF Clone
CAT＃: RG218329
- TrueORF®
KRT74 (tGFP-tagged) - Human keratin 74 (KRT74)
ORF Plasmid: DDK
"NM_175053" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADWH; HTSS2; HYPT3; K6IRS4; KRT5C; KRT6IRS4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218329 representing NM_175053
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCGGCAACTGAACATCAAGTCCAGTGGTGACAAGGGCAACTTCAGTGTGCATTCGGCAGTGGTGC CAAGGAAGGCTGTGGGTAGCCTGGCTTCTTACTGTGCAGCTGGCAGAGGGGCTGGCGCTGGCTTTGGCAG TCGGAGCCTCTATAGCCTTGGAGGGAATCGGCGTATTTCTTTCAATGTGGCTGGTGGCGGCGTTCGGGCT GGAGGTTACGGCTTCAGGCCTGGCTCTGGGTATGGAGGGGGCCGGGCCAGTGGCTTTGCTGGCAGTATGT TTGGCAGTGTGGCCCTGGGGCCTGCATGTTTGTCTGTGTGCCCACCTGGGGGCATCCACCAGGTCACTGT CAACAAGAGCCTCTTGGCCCCCCTCAACGTGGAGCTGGACCCTGAGATCCAGAAGGTGCGCGCCCAGGAG CGGGAACAGATCAAGGTGCTGAACGACAAGTTCGCCTCCTTCATTGACAAGGTACGCTTCCTAGAGCAGC AGAACCAGGTTCTAGAAACCAAGTGGGAGCTGCTGCAGCAGCTGGACCTGAACAACTGCAAGAAGAACCT GGAGCCCATCCTTGAGGGCTACATCAGCAACCTGCGGAAGCAGCTGGAGACACTGTCTGGGGACAGGGTG AGGCTGGACTCGGAGCTGAGAAGCATGAGGGATCTGGTGGAGGACTATAAGAAGAGATATGAGGTGGAGA TTAACCGGCGCACAACAGCAGAGAATGAGTTTGTGGTGCTTAAGAAGGATGCAGATGCAGCCTACGCAGT CAAGGTGGAGCTTCAGGCCAAAGTGGACTCACTGGACAAAGACATCAAGTTCCTCAAGTGTCTGTATGAT GCAGAGATCGCTCAGATCCAGACTCACGCCAGTGAGACCTCTGTCATCCTGTCCATGGACAACAACCGGG ACCTGGACCTTGACAGCATCATCGCTGAGGTCCGCATGCATTATGAGGAGATCGCCCTGAAGAGCAAGGC CGAGGCCGAGGCCCTGTACCAGACCAAGATCCAGGAGCTGCAGCTGGCAGCCAGTCGGCATGGTGACGAC CTGAAACACACCAGGAGCGAGATGGTGGAGCTGAACCGGCTCATCCAGAGGATCCGGTGTGAGATCGGGA ATGTGAAGAAGCAGCGTGCCAGCCTGGAGACGGCCATCGCTGACGCTGAGCAGCGGGGAGACAATGCCCT GAAGGATGCCCAGGCCAAGCTGGATGAGCTGGAGGGCGCCCTGCACCAGGCCAAGGAGGAGCTGGCGCGG ATGCTGCGCGAGTACCAGGAGCTCATGAGCCTGAAACTGGCCCTGGACATGGAGATTGCCACCTACCGCA AGCTGCTGGAGGGCGAGGAGTGCAGGATGTCTGGTGAGAATCCATCCTCTGTGAGCATCTCTGTCATCAG CAGTAGCAGCTACAGCTACCACCACCCCAGCTCTGCGGGTGTTGACCTTGGGGCCAGCGCTGTGGCAGGC AGCTCTGGCAGCACCCAGAGCGGGCAGACCAAGACCACAGAGGCGCGAGGGGGAGACCTCAAGGACACCC AGGGCAAGAGCACCCCAGCCAGCATCCCAGCAAGGAAAGCCACCCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218329 representing NM_175053
Red=Cloning site Green=Tags(s) MSRQLNIKSSGDKGNFSVHSAVVPRKAVGSLASYCAAGRGAGAGFGSRSLYSLGGNRRISFNVAGGGVRA GGYGFRPGSGYGGGRASGFAGSMFGSVALGPACLSVCPPGGIHQVTVNKSLLAPLNVELDPEIQKVRAQE REQIKVLNDKFASFIDKVRFLEQQNQVLETKWELLQQLDLNNCKKNLEPILEGYISNLRKQLETLSGDRV RLDSELRSMRDLVEDYKKRYEVEINRRTTAENEFVVLKKDADAAYAVKVELQAKVDSLDKDIKFLKCLYD AEIAQIQTHASETSVILSMDNNRDLDLDSIIAEVRMHYEEIALKSKAEAEALYQTKIQELQLAASRHGDD LKHTRSEMVELNRLIQRIRCEIGNVKKQRASLETAIADAEQRGDNALKDAQAKLDELEGALHQAKEELAR MLREYQELMSLKLALDMEIATYRKLLEGEECRMSGENPSSVSISVISSSSYSYHHPSSAGVDLGASAVAG SSGSTQSGQTKTTEARGGDLKDTQGKSTPASIPARKATR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175053 |
| ORF Size | 1587 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175053.2, NP_778223.1 |
| RefSeq Size | 2856 bp |
| RefSeq ORF | 1590 bp |
| Locus ID | 121391 |
| UniProt ID | Q7RTS7 |
| Gene Summary | Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This protein belongs to a family of keratins that are specifically expressed in the inner root sheath of hair follicles.[provided by RefSeq, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218329 | KRT74 (Myc-DDK-tagged)-Human keratin 74 (KRT74) |
CNY 3,936.00 |
|
| RC218329L3 | Lenti ORF clone of Human keratin 74 (KRT74), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC218329L4 | Lenti ORF clone of Human keratin 74 (KRT74), mGFP tagged |
CNY 6,460.00 |
|
| SC306983 | KRT74 (untagged)-Human keratin 74 (KRT74) |
CNY 8,550.00 |
