ZSCAN2 (NM_181877) Human Tagged ORF Clone
CAT#: RG218021
- TrueORF®
ZSCAN2 (tGFP-tagged) - Human zinc finger and SCAN domain containing 2 (ZSCAN2), transcript variant 1. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_181877" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ZFP29; ZNF854 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG218021 ORF sequence, codon optimized.
Due to the complexity of NM_181877, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCCGCAGATATTCCAAGGGTCACCACACCCCTGTCTAGCTTGGTGCAGGTGCCTCAGGAGGAGG ACAGGCAGGAGGAGGAGGTCACAACGATGATACTCGAAGATGACTCCTGGGTGCAGGAGGCCGTCCTTCA AGAGGATGGTCCAGAGAGTGAACCATTCCCACAGTCAGCGGGCAAAGGTGGACCCCAAGAGGAAGTGACT AGAGGGCCCCAGGGTGCTCTCGGACGCCTCCGAGAGCTGTGCAGGCGGTGGCTGAGGCCCGAGGTGCACA CCAAGGAGCAAATGCTCACTATGTTGCCCAAGGAGATCCAAGCATGGCTGCAGGAGCACCGCCCTGAGTC TTCTGAGGAAGCTGCCGCTCTGGTTGAAGATCTTACCCAGACCCTGCAGGATAGCGATTTCGAGATTCAG TCCGAGAACGGAGAGAATTGTAATCAGGACATGTTTGAGAACGAGTCCCGGAAAATCTTCTCCGAGATGC CTGAAGGTGAGTCAGCCCAGCATAGTGACGGAGAGAGCGACTTCGAAAGGGACGCAGGAATCCAGCGCCT CCAAGGACACTCCCCAGGCGAAGACCACGGCGAGGTGGTGAGTCAAGACCGAGAAGTGGGCCAGTTGATC GGGCTGCAGGGGACATACCTCGGTGAGAAGCCTTATGAATGCCCCCAGTGTGGAAAGACATTCAGCAGGA AATCCCATCTGATTACCCATGAGCGGACGCACACGGGAGAGAAATACTATAAGTGTGACGAATGTGGCAA GAGTTTTAGCGATGGCAGTAACTTCTCCAGGCACCAGACAACACACACTGGAGAGAAGCCCTACAAGTGT CGCGACTGTGGTAAGTCATTTAGCCGCAGCGCCAACCTGATCACGCATCAGCGAATTCATACTGGAGAGA AGCCGTTCCAGTGCGCGGAGTGCGGAAAATCCTTTTCACGATCTCCTAATCTGATCGCACACCAGAGGAC CCATACAGGTGAAAAGCCTTATTCCTGTCCTGAGTGCGGGAAATCATTTGGGAATCGCAGCTCTCTGAAC ACCCACCAAGGCATTCATACCGGGGAGAAGCCATACGAGTGTAAAGAGTGCGGCGAATCATTTTCTTATA ATTCCAACCTGATTAGGCACCAGCGAATTCATACAGGGGAAAAGCCTTACAAGTGTACAGACTGTGGGCA ACGCTTCTCACAAAGTTCTGCTCTCATCACCCACCGGAGGACTCACACCGGAGAAAAGCCTTACCAGTGC AGTGAGTGCGGGAAATCATTTTCAAGATCCAGCAACTTGGCTACCCACAGGCGCACCCATATGGTGGAGA AACCATATAAGTGCGGTGTGTGTGGGAAGAGTTTTAGTCAGTCCTCCTCCCTGATTGCACACCAGGGAAT GCATACCGGAGAGAAGCCCTACGAGTGCCTGACGTGCGGGGAGAGCTTCAGTTGGAGCTCCAACTTGCTT AAACACCAGCGAATTCATACCGGTGAGAAGCCCTATAAATGCTCTGAATGCGGAAAGTGCTTTTCCCAGC GCTCACAGCTGGTCGTGCACCAGAGAACTCATACAGGAGAAAAGCCGTACAAGTGCCTCATGTGCGGCAA AAGCTTCAGCAGGGGGTCTATACTCGTTATGCATCAGCGGGCTCACTTGGGCGACAAGCCCTACCGGTGC CCCGAATGTGGGAAAGGCTTTTCCTGGAATTCAGTTCTGATCATTCACCAGCGGATTCACACTGGTGAAA AGCCGTACAAATGTCCTGAGTGCGGGAAAGGGTTTAGTAATAGCAGTAATTTTATCACGCACCAAAGAAC TCATATGAAGGAGAAGCTCTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218021 representing NM_181877
Red=Cloning site Green=Tags(s) MMAADIPRVTTPLSSLVQVPQEEDRQEEEVTTMILEDDSWVQEAVLQEDGPESEPFPQSAGKGGPQEEVT RGPQGALGRLRELCRRWLRPEVHTKEQMLTMLPKEIQAWLQEHRPESSEEAAALVEDLTQTLQDSDFEIQ SENGENCNQDMFENESRKIFSEMPEGESAQHSDGESDFERDAGIQRLQGHSPGEDHGEVVSQDREVGQLI GLQGTYLGEKPYECPQCGKTFSRKSHLITHERTHTGEKYYKCDECGKSFSDGSNFSRHQTTHTGEKPYKC RDCGKSFSRSANLITHQRIHTGEKPFQCAECGKSFSRSPNLIAHQRTHTGEKPYSCPECGKSFGNRSSLN THQGIHTGEKPYECKECGESFSYNSNLIRHQRIHTGEKPYKCTDCGQRFSQSSALITHRRTHTGEKPYQC SECGKSFSRSSNLATHRRTHMVEKPYKCGVCGKSFSQSSSLIAHQGMHTGEKPYECLTCGESFSWSSNLL KHQRIHTGEKPYKCSECGKCFSQRSQLVVHQRTHTGEKPYKCLMCGKSFSRGSILVMHQRAHLGDKPYRC PECGKGFSWNSVLIIHQRIHTGEKPYKCPECGKGFSNSSNFITHQRTHMKEKLY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181877 |
ORF Size | 1842 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181877.3, NP_870992.2 |
RefSeq Size | 3756 bp |
RefSeq ORF | 1845 bp |
Locus ID | 54993 |
UniProt ID | Q7Z7L9 |
Protein Families | Transcription Factors |
Gene Summary | The protein encoded by this gene contains several copies of zinc finger motif, which is commonly found in transcriptional regulatory proteins. Studies in mice show that this gene is expressed during embryonic development, and specifically in the testis in adult mice, suggesting that it may play a role in regulating genes in germ cells. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |