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Collagen IV (COL4A2) (NM_001846) Human Tagged ORF Clone
CAT＃: RG217846
- TrueORF®
COL4A2 (tGFP-tagged) - Human collagen, type IV, alpha 2 (COL4A2)
ORF Plasmid: DDK
"NM_001846" in other vectors (2)
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CNY 18,810.00
货期*
4周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BSVD2; ICH; POREN2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217846 representing NM_001846
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGAGACCAGCGCGCGGTGGCCGGCCCTGCCCTACGGCGGTGGCTGCTGCTGGGGACAGTGACCG TGGGGTTCCTCGCCCAGAGCGTCTTGGCGGGTGTGAAGAAGTTTGATGTGCCGTGTGGAGGAAGAGATTG CAGTGGGGGCTGCCAGTGCTACCCTGAGAAAGGTGGACGTGGTCAGCCTGGGCCAGTGGGCCCCCAGGGG TACAATGGGCCACCAGGATTACAAGGATTCCCCGGGCTGCAGGGACGTAAAGGAGACAAGGGTGAAAGGG GAGCCCCCGGAGTAACAGGACCCAAGGGCGACGTGGGAGCAAGAGGCGTTTCTGGATTCCCTGGTGCCGA TGGAATTCCTGGACACCCGGGGCAAGGTGGGCCCAGGGGAAGGCCGGGCTACGATGGCTGCAACGGAACC CAGGGAGACTCAGGTCCACAGGGGCCCCCCGGCTCTGAGGGGTTCACCGGGCCTCCCGGGCCCCAAGGAC CAAAAGGGCAGAAAGGTGAGCCTTATGCACTGCCTAAAGAGGAGCGCGACAGATATCGGGGTGAACCTGG AGAGCCTGGATTGGTCGGTTTCCAGGGACCTCCCGGCCGCCCTGGGCATGTGGGACAGATGGGTCCAGTT GGAGCTCCAGGGAGACCAGGACCACCTGGACCCCCTGGACCAAAAGGACAGCAAGGCAACAGAGGACTTG GTTTCTACGGAGTTAAGGGTGAAAAGGGTGACGTAGGGCAGCCGGGACCCAACGGGATTCCATCAGACAC CCTCCACCCCATCATCGCGCCCACAGGAGTCACCTTCCACCCAGATCAGTACAAGGGTGAAAAAGGCAGT GAGGGGGAACCAGGAATAAGAGGCATTTCCTTGAAGGGAGAAGAAGGAATCATGGGCTTTCCTGGACTTA GGGGTTACCCTGGCTTGAGTGGTGAAAAAGGATCACCAGGACAGAAGGGAAGCCGAGGCCTGGATGGCTA TCAAGGGCCTGATGGACCCCGGGGACCTAAGGGAGAAGCCGGAGACCCAGGGCCCCCTGGACTACCTGCC TACTCCCCTCACCCTTCCCTAGCAAAAGGTGCCAGAGGTGACCCAGGATTCCCAGGGGCCCAAGGGGAGC CAGGAAGCCAGGGTGAGCCAGGAGACCCGGGCCTCCCAGGTCCCCCTGGCCTCTCCATTGGAGATGGAGA TCAGAGGAGAGGCCTGCCGGGTGAGATGGGACCCAAGGGCTTCATCGGAGACCCCGGCATCCCTGCGCTC TACGGGGGCCCACCTGGACCTGATGGAAAGCGAGGGCCTCCAGGACCCCCCGGGCTCCCTGGACCACCTG GACCTGATGGCTTCCTGTTTGGGCTGAAAGGAGCAAAAGGAAGAGCAGGCTTCCCTGGGCTTCCCGGCTC CCCTGGAGCCCCCGGACCAAAGGGGTGGAAAGGTGACGCTGGGGAATGCAGATGTACAGAAGGCGACGAA GCTATCAAAGGTCTTCCAGGACTGCCAGGACCCAAGGGCTTCGCAGGCATCAACGGGGAGCCGGGGAGGA AAGGGGACAAAGGAGACCCCGGCCAACACGGCCTCCCTGGGTTCCCAGGGCTCAAGGGAGTGCCTGGCAA CATTGGTGCTCCCGGACCCAAAGGAGCAAAAGGAGATTCCAGAACAATCACAACCAAAGGTGAGCGGGGA CAGCCCGGCGTCCCAGGTGTGCCCGGGATGAAAGGTGACGATGGCAGCCCAGGCCGCGATGGGCTCGATG GATTCCCCGGCCTCCCAGGCCCTCCCGGTGATGGCATCAAGGGCCCTCCAGGGGACCCAGGTTATCCAGG AATACCTGGAACGAAGGGTACTCCAGGAGAAATGGGCCCCCCAGGACTGGGCCTTCCCGGCCTCAAAGGC CAACGTGGTTTCCCTGGAGACGCCGGCTTACCTGGACCACCAGGCTTCCTGGGCCCTCCTGGCCCCGCAG GGACCCCAGGACAAATAGATTGTGACACAGATGTGAAAAGGGCCGTTGGAGGTGACAGACAGGAGGCCAT CCAGCCAGGTTGCATAGGAGGGCCCAAGGGATTGCCAGGCCTGCCAGGACCCCCAGGCCCCACAGGTGCC AAAGGCCTCCGAGGAATCCCAGGCTTCGCAGGAGCTGATGGAGGACCAGGGCCCAGGGGCTTGCCAGGAG ACGCAGGTCGTGAAGGGTTCCCAGGACCCCCAGGGTTCATAGGACCCCGAGGATCCAAAGGTGCAGTGGG CCTCCCTGGCCCAGATGGATCCCCAGGTCCCATCGGCCTGCCAGGGCCAGATGGGCCCCCTGGGGAAAGG GGCCTCCCTGGAGAAGTCCTGGGAGCTCAGCCCGGGCCACGGGGAGATGCTGGTGTGCCTGGACAGCCTG GGCTTAAAGGCCTTCCCGGAGACAGAGGCCCCCCTGGATTCAGAGGAAGCCAAGGGATGCCTGGGATGCC AGGGCTGAAGGGCCAGCCAGGCCTCCCAGGACCTTCCGGCCAGCCAGGCCTGTATGGGCCTCCAGGACTG CATGGATTCCCAGGAGCTCCTGGCCAAGAGGGGCCCTTGGGGCTGCCAGGAATCCCAGGCCGTGAAGGTC TGCCTGGTGATAGAGGGGACCCTGGGGACACAGGCGCTCCTGGCCCTGTGGGCATGAAAGGTCTCTCTGG TGACAGAGGAGATGCTGGCTTCACAGGGGAGCAAGGCCATCCAGGAAGCCCTGGATTTAAAGGAATTGAT GGAATGCCTGGGACCCCCGGGCTAAAAGGAGATAGAGGCTCACCTGGGATGGATGGTTTCCAAGGCATGC CTGGACTCAAAGGGAGACCCGGGTTTCCAGGGAGCAAAGGCGAGGCTGGATTTTTCGGAATACCCGGTCT GAAGGGTCTGGCTGGTGAGCCAGGTTTTAAAGGCAGCCGAGGGGACCCTGGGCCCCCAGGACCACCTCCT GTCATCCTGCCAGGAATGAAAGACATTAAAGGAGAGAAAGGAGATGAAGGGCCTATGGGGCTGAAAGGAT ACCTGGGCGCAAAAGGTATCCAAGGAATGCCAGGCATCCCAGGGCTGTCAGGAATCCCTGGGCTGCCTGG GAGGCCCGGCCACATCAAAGGAGTCAAGGGAGACATCGGAGTCCCCGGCATCCCCGGTTTGCCAGGATTC CCTGGGGTGGCTGGCCCCCCTGGAATTACGGGATTCCCAGGATTCATAGGAAGCCGGGGTGACAAAGGTG CCCCAGGGAGAGCAGGCCTGTATGGCGAGATTGGCGCGACTGGTGATTTCGGTGACATCGGGGACACTAT AAATTTACCAGGAAGACCAGGCCTGAAGGGGGAGCGGGGCACCACTGGAATACCAGGTCTGAAGGGATTC TTTGGAGAGAAGGGAACAGAAGGTGACATCGGCTTCCCTGGGATAACAGGCGTGACTGGAGTCCAAGGCC CTCCTGGACTTAAAGGACAAACAGGCTTTCCAGGGCTGACTGGGCCTCCAGGGTCGCAGGGAGAGCTGGG GCGGATTGGACTGCCTGGTGGCAAAGGAGATGATGGCTGGCCGGGAGCTCCGGGCTTACCAGGTTTTCCG GGACTCCGTGGGATCCGCGGCTTACACGGCTTGCCAGGCACCAAGGGCTTTCCAGGATCCCCAGGTTCTG ACATCCACGGAGACCCAGGCTTCCCAGGCCCTCCTGGGGAAAGAGGTGACCCAGGAGAGGCCAACACCCT TCCAGGCCCTGTGGGAGTCCCAGGACAGAAAGGAGACCAAGGAGCTCCAGGGGAACGAGGCCCACCTGGG AGCCCAGGACTTCAGGGGTTCCCAGGCATCACACCCCCTTCCAACATCTCTGGGGCACCTGGTGACAAAG GGGCGCCAGGGATATTTGGCCTGAAAGGTTATCGGGGCCCACCAGGGCCACCAGGTTCTGCTGCTCTTCC TGGAAGCAAAGGTGACACAGGGAACCCAGGAGCTCCAGGAACCCCAGGGACCAAAGGATGGGCCGGGGAC TCCGGGCCCCAGGGCAGGCCTGGTGTGTTTGGTCTCCCAGGAGAAAAAGGGCCCAGGGGTGAACAAGGCT TCATGGGGAACACTGGACCCACCGGGGCGGTGGGCGACAGAGGCCCCAAGGGACCCAAGGGAGACCCAGG ATTCCCTGGTGCCCCCGGGACTGTGGGAGCCCCCGGGATTGCAGGAATCCCCCAGAAGATTGCCGTCCAA CCAGGGACAGTGGGTCCCCAGGGGAGGCGAGGCCCCCCTGGGGCACCGGGGGAGATGGGGCCCCAGGGCC CCCCCGGAGAACCAGGTTTTCGTGGGGCTCCAGGGAAAGCTGGGCCCCAAGGAAGAGGTGGTGTGTCTGC TGTTCCCGGCTTCCGGGGAGATGAAGGACCCATAGGCCACCAGGGGCCGATTGGCCAAGAAGGTGCACCA GGCCGTCCAGGGAGCCCGGGCCTGCCGGGTATGCCAGGCCGCAGCGTCAGCATCGGCTACCTCCTGGTGA AGCACAGCCAGACGGACCAGGAGCCCATGTGCCCGGTGGGCATGAACAAACTCTGGAGTGGATACAGCCT GCTGTACTTCGAGGGCCAGGAGAAGGCGCACAACCAGGACCTGGGGCTGGCGGGCTCCTGCCTGGCGCGG TTCAGCACCATGCCCTTCCTGTACTGCAACCCTGGTGATGTCTGCTACTATGCCAGCCGGAACGACAAGT CCTACTGGCTCTCTACCACTGCGCCGCTGCCCATGATGCCCGTGGCCGAGGACGAGATCAAGCCCTACAT CAGCCGCTGTTCTGTGTGTGAGGCCCCGGCCATCGCCATCGCGGTCCACAGTCAGGATGTCTCCATCCCA CACTGCCCAGCTGGGTGGCGGAGTTTGTGGATCGGATATTCCTTCCTCATGCACACGGCGGCGGGAGACG AAGGCGGTGGCCAATCACTGGTGTCACCGGGCAGCTGTCTAGAGGACTTCCGCGCCACACCATTCATCGA ATGCAATGGAGGCCGCGGCACCTGCCACTACTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCC GAGCAGAGCTTCCAGGGCTCGCCCTCCGCCGACACGCTCAAGGCCGGCCTCATCCGCACACACATCAGCC GCTGCCAGGTGTGCATGAAGAACCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217846 representing NM_001846
Red=Cloning site Green=Tags(s) MGRDQRAVAGPALRRWLLLGTVTVGFLAQSVLAGVKKFDVPCGGRDCSGGCQCYPEKGGRGQPGPVGPQG YNGPPGLQGFPGLQGRKGDKGERGAPGVTGPKGDVGARGVSGFPGADGIPGHPGQGGPRGRPGYDGCNGT QGDSGPQGPPGSEGFTGPPGPQGPKGQKGEPYALPKEERDRYRGEPGEPGLVGFQGPPGRPGHVGQMGPV GAPGRPGPPGPPGPKGQQGNRGLGFYGVKGEKGDVGQPGPNGIPSDTLHPIIAPTGVTFHPDQYKGEKGS EGEPGIRGISLKGEEGIMGFPGLRGYPGLSGEKGSPGQKGSRGLDGYQGPDGPRGPKGEAGDPGPPGLPA YSPHPSLAKGARGDPGFPGAQGEPGSQGEPGDPGLPGPPGLSIGDGDQRRGLPGEMGPKGFIGDPGIPAL YGGPPGPDGKRGPPGPPGLPGPPGPDGFLFGLKGAKGRAGFPGLPGSPGAPGPKGWKGDAGECRCTEGDE AIKGLPGLPGPKGFAGINGEPGRKGDKGDPGQHGLPGFPGLKGVPGNIGAPGPKGAKGDSRTITTKGERG QPGVPGVPGMKGDDGSPGRDGLDGFPGLPGPPGDGIKGPPGDPGYPGIPGTKGTPGEMGPPGLGLPGLKG QRGFPGDAGLPGPPGFLGPPGPAGTPGQIDCDTDVKRAVGGDRQEAIQPGCIGGPKGLPGLPGPPGPTGA KGLRGIPGFAGADGGPGPRGLPGDAGREGFPGPPGFIGPRGSKGAVGLPGPDGSPGPIGLPGPDGPPGER GLPGEVLGAQPGPRGDAGVPGQPGLKGLPGDRGPPGFRGSQGMPGMPGLKGQPGLPGPSGQPGLYGPPGL HGFPGAPGQEGPLGLPGIPGREGLPGDRGDPGDTGAPGPVGMKGLSGDRGDAGFTGEQGHPGSPGFKGID GMPGTPGLKGDRGSPGMDGFQGMPGLKGRPGFPGSKGEAGFFGIPGLKGLAGEPGFKGSRGDPGPPGPPP VILPGMKDIKGEKGDEGPMGLKGYLGAKGIQGMPGIPGLSGIPGLPGRPGHIKGVKGDIGVPGIPGLPGF PGVAGPPGITGFPGFIGSRGDKGAPGRAGLYGEIGATGDFGDIGDTINLPGRPGLKGERGTTGIPGLKGF FGEKGTEGDIGFPGITGVTGVQGPPGLKGQTGFPGLTGPPGSQGELGRIGLPGGKGDDGWPGAPGLPGFP GLRGIRGLHGLPGTKGFPGSPGSDIHGDPGFPGPPGERGDPGEANTLPGPVGVPGQKGDQGAPGERGPPG SPGLQGFPGITPPSNISGAPGDKGAPGIFGLKGYRGPPGPPGSAALPGSKGDTGNPGAPGTPGTKGWAGD SGPQGRPGVFGLPGEKGPRGEQGFMGNTGPTGAVGDRGPKGPKGDPGFPGAPGTVGAPGIAGIPQKIAVQ PGTVGPQGRRGPPGAPGEMGPQGPPGEPGFRGAPGKAGPQGRGGVSAVPGFRGDEGPIGHQGPIGQEGAP GRPGSPGLPGMPGRSVSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLAR FSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIP HCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIP EQSFQGSPSADTLKAGLIRTHISRCQVCMKNL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001846 |
| ORF Size | 5136 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001846.1, NP_001837.1 |
| RefSeq Size | 6276 bp |
| RefSeq ORF | 5139 bp |
| Locus ID | 1284 |
| UniProt ID | P08572 |
| Protein Families | Transmembrane |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
| Gene Summary | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008] |
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