ASAH3L (ACER2) (NM_001010887) Human Tagged ORF Clone
CAT#: RG217746
- TrueORF®
ACER2 (tGFP-tagged) - Human alkaline ceramidase 2 (ACER2)
ORF Plasmid: DDK
"NM_001010887" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 4,000.00
CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ALKCDase2; ASAH3L |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217746 representing NM_001010887
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCCCCGCACTGGTGGGACCAGCTGCAGGCTGGTAGCTCGGAGGTGGACTGGTGCGAGGACAACT ACACCATCGTGCCTGCTATCGCCGAGTTCTACAACACGATCAGCAATGTCTTATTTTTCATTTTACCGCC CATCTGCATGTGCTTGTTTCGTCAGTATGCAACATGCTTCAACAGTGGCATCTACTTAATCTGGACTCTT TTGGTTGTAGTGGGAATTGGATCCGTCTACTTCCATGCAACCCTTAGTTTCTTGGGTCAGATGCTTGATG AACTTGCAGTCCTTTGGGTTCTGATGTGTGCTTTGGCCATGTGGTTCCCCAGAAGGTATCTACCAAAGAT CTTTCGGAATGACCGGGGTAGGTTCAAGGTGGTGGTCAGTGTCCTGTCTGCGGTTACGACGTGCCTGGCA TTTGTCAAGCCTGCCATCAACAACATCTCTCTGATGACCCTGGGAGTTCCTTGCACTGCACTGCTCATCG CAGAGCTAAAGAGGTGTGACAACATGCGTGTGTTTAAGCTGGGCCTCTTCTCGGGCCTCTGGTGGACCCT GGCCCTGTTCTGCTGGATCAGTGACCGAGCTTTCTGCGAGCTGCTGTCATCCTTCAACTTCCCCTACCTG CACTGCATGTGGCACATCCTCATCTGCCTTGCTGCCTACCTGGGCTGTGTATGCTTTGCCTACTTTGATG CTGCCTCAGAGATTCCTGAGCAAGGCCCTGTCATCAAGTTCTGGCCCAATGAGAAATGGGCCTTCATTGG TGTCCCCTATGTGTCCCTCCTGTGTGCCAACAAGAAATCATCAGTCAAGATCACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217746 representing NM_001010887
Red=Cloning site Green=Tags(s) MGAPHWWDQLQAGSSEVDWCEDNYTIVPAIAEFYNTISNVLFFILPPICMCLFRQYATCFNSGIYLIWTL LVVVGIGSVYFHATLSFLGQMLDELAVLWVLMCALAMWFPRRYLPKIFRNDRGRFKVVVSVLSAVTTCLA FVKPAINNISLMTLGVPCTALLIAELKRCDNMRVFKLGLFSGLWWTLALFCWISDRAFCELLSSFNFPYL HCMWHILICLAAYLGCVCFAYFDAASEIPEQGPVIKFWPNEKWAFIGVPYVSLLCANKKSSVKIT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001010887 |
ORF Size | 825 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001010887.3 |
RefSeq Size | 2852 bp |
RefSeq ORF | 828 bp |
Locus ID | 340485 |
UniProt ID | Q5QJU3 |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
Gene Summary | The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217746 | ACER2 (Myc-DDK-tagged)-Human alkaline ceramidase 2 (ACER2) |
CNY 2,400.00 |
|
RC217746L1 | Lenti ORF clone of Human alkaline ceramidase 2 (ACER2), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC217746L2 | Lenti ORF clone of Human alkaline ceramidase 2 (ACER2), mGFP tagged |
CNY 5,890.00 |
|
RC217746L3 | Lenti ORF clone of Human alkaline ceramidase 2 (ACER2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC217746L4 | Lenti ORF clone of Human alkaline ceramidase 2 (ACER2), mGFP tagged |
CNY 4,800.00 |
|
SC301506 | ACER2 (untagged)-Human alkaline ceramidase 2 (ACER2) |
CNY 6,270.00 |