PRMT4 (CARM1) (NM_199141) Human Tagged ORF Clone
CAT#: RG217483
- TrueORF®
CARM1 (tGFP-tagged) - Human coactivator-associated arginine methyltransferase 1 (CARM1)
ORF Plasmid: DDK
"NM_199141" in other vectors (6)
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CNY 8,432.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PRMT4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217483 representing NM_199141
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGCGGCGGCGGCGGTGGGGCCGGGCGCGGGCGGCGCGGGGTCGGCGGTCCCGGGCGGCGCGG GGCCCTGCGCTACCGTGTCGGTGTTCCCCGGCGCCCGCCTCCTCACCATCGGCGACGCGAACGGCGAGAT CCAGCGGCACGCGGAGCAGCAGGCGCTGCGCCTCGAGGTGCGCGCCGGCCCGGACTCGGCGGGCATCGCC CTCTACAGCCATGAAGATGTGTGTGTCTTTAAGTGCTCAGTGTCCCGAGAGACAGAGTGCAGCCGTGTGG GCAAGCAGTCCTTCATCATCACCCTGGGCTGCAACAGCGTCCTCATCCAGTTCGCCACACCCAACGATTT CTGTTCCTTCTACAACATCCTGAAAACCTGCCGGGGCCACACCCTGGAGCGGTCTGTGTTCAGCGAGCGG ACGGAGGAGTCTTCTGCCGTGCAGTACTTCCAGTTTTATGGCTACCTGTCCCAGCAGCAGAACATGATGC AGGACTACGTGCGGACAGGCACCTACCAGCGCGCCATCCTGCAAAACCACACCGACTTCAAGGACAAGAT CGTTCTTGATGTTGGCTGTGGCTCTGGGATCCTGTCGTTTTTTGCCGCCCAAGCTGGAGCACGGAAAATC TACGCGGTGGAGGCCAGCACCATGGCCCAGCACGCTGAGGTCTTGGTGAAGAGTAACAACCTGACGGACC GCATCGTGGTCATCCCGGGCAAGGTGGAGGAGGTGTCACTCCCCGAGCAGGTGGACATCATCATCTCGGA GCCCATGGGCTACATGCTCTTCAACGAGCGCATGCTGGAGAGCTACCTCCACGCCAAGAAGTACCTGAAG CCCAGCGGAAACATGTTTCCTACCATTGGTGACGTCCACCTTGCACCCTTCACGGATGAACAGCTCTACA TGGAGCAGTTCACCAAGGCCAACTTCTGGTACCAGCCATCTTTCCATGGAGTGGACCTGTCGGCCCTCCG AGGTGCCGCGGTGGATGAGTATTTCCGGCAGCCTGTGGTGGACACATTTGACATCCGGATCCTGATGGCC AAGTCTGTCAAGTACACGGTGAACTTCTTAGAAGCCAAAGAAGGAGATTTGCACAGGATAGAAATCCCAT TCAAATTCCACATGCTGCATTCAGGGCTGGTCCACGGCCTGGCTTTCTGGTTTGACGTTGCTTTCATCGG CTCCATAATGACCGTGTGGCTGTCCACAGCCCCGACAGAGCCCCTGACCCACTGGTACCAGGTGCGGTGC CTGTTCCAGTCACCACTGTTCGCCAAGGCAGGGGACACGCTCTCAGGGACATGTCTGCTTATTGCCAACA AAAGACAGAGCTACGACATCAGTATTGTGGCCCAGGTGGACCAGACCGGCTCCAAGTCCAGTAACCTCCT GGATCTGAAAAACCCCTTCTTTAGATACACGGGCACAACGCCCTCACCCCCACCCGGCTCCCACTACACA TCTCCCTCGGAAAACATGTGGAACACGGGCAGCACCTACAACCTCAGCAGCGGGATGGCCGTGGCAGGGA TGCCGACCGCCTATGACTTGAGCAGTGTTATTGCCAGTGGCTCCAGCGTGGGCCACAACAACCTGATTCC TTTAGCCAACACGGGGATTGTCAATCACACCCACTCCCGGATGGGCTCCATAATGAGCACGGGGATTGTC CAAGGGTCCTCCGGCGCCCAGGGCAGTGGTGGTGGCAGCACGAGTGCCCACTATGCAGTCAACAGCCAGT TCACCATGGGCGGCCCCGCCATCTCCATGGCGTCGCCCATGTCCATCCCGACCAACACCATGCACTACGG GAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_199141 |
ORF Size | 1824 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199141.2 |
RefSeq Size | 2968 bp |
RefSeq ORF | 1827 bp |
Locus ID | 10498 |
UniProt ID | Q86X55 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217483 | CARM1 (Myc-DDK-tagged)-Human coactivator-associated arginine methyltransferase 1 (CARM1) |
CNY 6,832.00 |
|
RC217483L1 | Lenti ORF clone of Human coactivator-associated arginine methyltransferase 1 (CARM1), Myc-DDK-tagged |
CNY 9,232.00 |
|
RC217483L2 | Lenti ORF clone of Human coactivator-associated arginine methyltransferase 1 (CARM1), mGFP tagged |
CNY 7,220.00 |
|
RC217483L3 | Lenti ORF clone of Human coactivator-associated arginine methyltransferase 1 (CARM1), Myc-DDK-tagged |
CNY 9,232.00 |
|
RC217483L4 | Lenti ORF clone of Human coactivator-associated arginine methyltransferase 1 (CARM1), mGFP tagged |
CNY 7,220.00 |
|
SC121581 | CARM1 (untagged)-Human coactivator-associated arginine methyltransferase 1 (CARM1) |
CNY 6,800.00 |