B7-2 (CD86) (NM_175862) Human Tagged ORF Clone
CAT#: RG217341
- TrueORF®
CD86 (tGFP-tagged) - Human CD86 molecule (CD86), transcript variant 1
ORF Plasmid: DDK
"NM_175862" in other vectors (6)
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CNY 5,200.00
Cited in 3 publications. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | B7-2; B7.2; B70; CD28LG2; LAB72 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217341 representing NM_175862
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCCAGTGCACTATGGGACTGAGTAACATTCTCTTTGTGATGGCCTTCCTGCTCTCTGGTGCTG CTCCTCTGAAGATTCAAGCTTATTTCAATGAGACTGCAGACCTGCCATGCCAATTTGCAAACTCTCAAAA CCAAAGCCTGAGTGAGCTAGTAGTATTTTGGCAGGACCAGGAAAACTTGGTTCTGAATGAGGTATACTTA GGCAAAGAGAAATTTGACAGTGTTCATTCCAAGTATATGGGCCGCACAAGTTTTGATTCGGACAGTTGGA CCCTGAGACTTCACAATCTTCAGATCAAGGACAAGGGCTTGTATCAATGTATCATCCATCACAAAAAGCC CACAGGAATGATTCGCATCCACCAGATGAATTCTGAACTGTCAGTGCTTGCTAACTTCAGTCAACCTGAA ATAGTACCAATTTCTAATATAACAGAAAATGTGTACATAAATTTGACCTGCTCATCTATACACGGTTACC CAGAACCTAAGAAGATGAGTGTTTTGCTAAGAACCAAGAATTCAACTATCGAGTATGATGGTATTATGCA GAAATCTCAAGATAATGTCACAGAACTGTACGACGTTTCCATCAGCTTGTCTGTTTCATTCCCTGATGTT ACGAGCAATATGACCATCTTCTGTATTCTGGAAACTGACAAGACGCGGCTTTTATCTTCACCTTTCTCTA TAGAGCTTGAGGACCCTCAGCCTCCCCCAGACCACATTCCTTGGATTACAGCTGTACTTCCAACAGTTAT TATATGTGTGATGGTTTTCTGTCTAATTCTATGGAAATGGAAGAAGAAGAAGCGGCCTCGCAACTCTTAT AAATGTGGAACCAACACAATGGAGAGGGAAGAGAGTGAACAGACCAAGAAAAGAGAAAAAATCCATATAC CTGAAAGATCTGATGAAGCCCAGCGTGTTTTTAAAAGTTCGAAGACATCTTCATGCGACAAAAGTGATAC ATGTTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217341 representing NM_175862
Red=Cloning site Green=Tags(s) MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYL GKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPE IVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDV TSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPRNSY KCGTNTMEREESEQTKKREKIHIPERSDEAQRVFKSSKTSSCDKSDTCF TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_175862 |
ORF Size | 987 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175862.4, NP_787058.4 |
RefSeq Size | 2781 bp |
RefSeq ORF | 990 bp |
Locus ID | 942 |
UniProt ID | P42081 |
Protein Families | Druggable Genome, Transcription Factors, Transmembrane |
Protein Pathways | Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis |
Gene Summary | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Live cell superresolution-structured illumination microscopy imaging analysis of the intercellular transport of microvesicles and costimulatory proteins via nanotubes between immune cells
,Halász, H;Ghadaksaz, A;Madarász, T;Huber, K;Harami, G;Tóth, E;Osteikoetxea-Molnár, A;Kovács, M;Balogi, Z;Nyitrai, M;Matkó, J;Szabó-Meleg, E;,
Methods Appl. Fluoresc.
,PubMed ID 30039805
[CD86]
|
Single-molecule diffusion-based estimation of GPCR activity
,Yanagawa, M;Hiroshima, M;Togashi, Y;Yamashita, T;Shichida, Y;Murata, M;Ueda, M;Sako, Y;,
bioRxiv
[CD86]
|
DOCK8 enforces immunological tolerance by promoting IL-2 signaling and immune synapse formation in Tregs
,Janssen, E;Kumari, S;Tohme, M;Ullas, S;Barrera, V;Tas, JM;Castillo-Rama, M;Bronson, RT;Usmani, SM;Irvine, DJ;Mempel, TR;Geha, RS;,
JCI Insight
,PubMed ID 28978806
[CD86]
|
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217341 | CD86 (Myc-DDK-tagged)-Human CD86 molecule (CD86), transcript variant 1 |
CNY 3,600.00 |
|
RC217341L1 | Lenti-ORF clone of CD86 (Myc-DDK-tagged)-Human CD86 molecule (CD86), transcript variant 1 |
CNY 6,000.00 |
|
RC217341L2 | Lenti-ORF clone of CD86 (mGFP-tagged)-Human CD86 molecule (CD86), transcript variant 1 |
CNY 6,000.00 |
|
RC217341L3 | Lenti-ORF clone of CD86 (Myc-DDK-tagged)-Human CD86 molecule (CD86), transcript variant 1 |
CNY 6,000.00 |
|
RC217341L4 | Lenti-ORF clone of CD86 (mGFP-tagged)-Human CD86 molecule (CD86), transcript variant 1 |
CNY 5,890.00 |
|
SC101350 | CD86 (untagged)-Human CD86 molecule (CD86), transcript variant 1 |
CNY 3,600.00 |