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HELB (NM_033647) Human Tagged ORF Clone
CAT＃: RG217186
- TrueORF®
HELB (tGFP-tagged) - Human helicase (DNA) B (HELB)
ORF Plasmid: DDK
"NM_033647" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DHB; hDHB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217186 representing NM_033647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGTCGAGTCCGTACCTGCGCCAACTTCAGGGACCTCTGCTCCCACCCAGGGATCTGGTGGAGG AGGACGACGACTACCTAAACGACGACGTGGAGGAGGATGAAGAGTCCGTGTTCATCGACGCCGAGGAGCT CTGCAGTGGGGGCGTAAAGGCTGGCAGCCTCCCCGGGTGCCTCCGCGTTTCTATTTGTGATGAAAACACA CAAGAGACATGTAAAGTGTTTGGACGTTTTCCGATAACAGGTGCTTGGTGGAGAGTGAAGGTACAAGTAA AGCCTGTGGTGGGATCAAGGAGCTATCAATATCAAGTTCAAGGATTTCCGTCTTACTTTTTGCAGTCTGA TATGTCACCACCAAATCAAAAACATATCTGTGCTCTCTTTCTTAAAGAGTGTGAGGTCTCCAGTGATGAT GTTAATAAATTTTTAACATGGGTAAAGGAGGTATCAAACTACAAAAACCTAAACTTTGAAAATCTTAGGG AAACACTAAGAACTTTCCACAAGGAAACTGGAAGGAAAGATCAAAAGCAGCCTACACAGAATGGTCAGGA AGAGTTGTTCCTAGACAATGAGATGAGTCTTCCTCTGGAAAACACAATTCCATTTAGAAATGTAATGACA GCTTTGCAGTTTCCGAAGATAATGGAATTCCTTCCAGTTCTTCTGCCTCGACACTTTAAATGGATCATAG GGTCAGGTTCTAAAGAGATGTTGAAAGAGATAGAAGAGATTTTAGGTACACATCCGTGGAAACTTGGATT TAGTAAAATAACCTACAGAGAGTGGAAACTCCTGCGATGTGAGGCAAGTTGGATAGCATTTTGTCAGTGT GAGTCTCTTCTCCAGCTGATGACTGATTTGGAGAAGAATGCATTAATAATGTATTCCAGACTGAAGCAGA TATGTAGAGAAGATGGGCACACATATGTTGAAGTGAATGACTTAACTTTGACATTGTCAAATCATATGTC ATTTCATGCTGCTTCAGAGTCTCTGAAGTTTTTGAAGGATATTGGTGTGGTGACATATGAGAAGTCCTGT GTCTTCCCTTATGACCTTTACCATGCTGAAAGAGCCATCGCCTTTTCAATTTGTGACCTGATGAAGAAAC CTCCTTGGCATTTATGTGTCGATGTCGAAAAGGTGCTTGCCTCTATTCACACCACAAAACCTGAGAATTC AAGCGATGATGCATTGAATGAGAGCAAACCTGATGAAGTAAGATTAGAAAATCCTGTGGATGTTGTGGAC ACACAGGACAATGGTGACCATATTTGGACTAATGGTGAAAATGAAATTAATGCAGAAATAAGTGAAGTTC AGCTGGATCAGGATCAGGTTGAAGTTCCACTGGATCGGGATCAGGTGGCTGCTTTGGAAATGATTTGCTC CAATCCTGTGACAGTCATAAGTGGGAAAGGTGGATGTGGGAAGACCACAATCGTTAGCCGTCTTTTTAAG CATATAGAGCAGTTGGAAGAAAGAGAAGTAAAAAAAGCCTGTGAAGATTTTGAACAAGACCAGAATGCTT CAGAAGAATGGATTACCTTTACTGAGCAAAGTCAACTAGAGGCGGACAAGGCTATAGAAGTTTTGCTCAC AGCACCTACAGGGAAAGCAGCTGGCTTACTAAGACAGAAAACTGGTCTTCATGCCTACACACTGTGTCAG GTCAATTATAGCTTCTATTCATGGACTCAAACAATGATGACCACAAACAAACCATGGAAATTTTCTTCGG TTAGAGTTCTGGTTGTGGATGAAGGGAGTTTGGTATCTGTAGGAATCTTCAAATCGGTCTTAAATTTATT GTGTGAGCACTCCAAACTTTCTAAGCTTATTATCCTTGGTGACATTAGACAGTTACCCAGTATTGAACCT GGTAACTTGCTGAAAGATCTTTTTGAGACTCTTAAGTCAAGAAATTGTGCTATTGAGCTAAAGACAAACC ATAGAGCAGAATCTCAGCTCATTGTGGACAATGCTACAAGAATCTCAAGACGCCAATTTCCAAAATTTGA TGCAGAACTAAATATCTCTGATAATCCAACATTACCCATCTCAATTCAAGATAAGACATTTATTTTTGTC AGGCTCCCAGAAGAGGATGCCAGTTCTCAGTCATCTAAAACTAATCATCACTCTTGTTTATATTCTGCAG TTAAAACTTTACTACAAGAAAATAACTTACAAAATGCAAAAACATCACAATTTATTGCATTTAGAAGGCA AGACTGTGATCTAATTAATGACTGCTGCTGCAAACACTACACAGGCCACCTCACCAAAGACCATCAGAGT AGACTTGTTTTTGGAATTGGTGATAAAATTTGTTGTACCAGGAATGCATACCTCTCAGACTTACTACCTG AAAATATCTCTGGAAGTCAGCAAAATAATGATCTAGATGCCAGTAGTGAAGACTTTTCTGGTACGCTTCC TGATTTTGCTAAAAATAAGCGTGACTTTGAAAGTAACGTTCGACTGTGCAATGGAGAGATATTTTTCATA ACAAATGATGTAACTGATGTAACTTTTGGAAAGAGAAGATCTTTGACCATTAATAATATGGCTGGCCTGG AAGTAACTGTGGATTTTAAGAAACTAATGAAATATTGTCGCATAAAACATGCATGGGCAAGAACTATTCA CACTTTTCAGGGGTCCGAGGAGCAAACAGTTGTCTATGTGGTGGGGAAGGCGGGCCGCCAGCACTGGCAG CATGTCTACACCGCCGTGACCAGGGGCCGCTGCCGAGTGTATGTGATTGCAGAGGAGTCTCAGCTCCGGA ATGCCATTATGAAAAACAGTTTTCCTAGAAAAACTCGTTTGAAACATTTCTTGCAAAGTAAGCTCTCCTC TAGCGGCGCACCTCCAGCAGATTTTCCGTCCCCACGGAAGAGCTCTGGAGACAGTGGAGGACCCAGCACA CCGTCAGCATCTCCACTCCCTGTAGTCACAGACCACGCCATGACAAATGATGTCACCTGGAGCGAGGCCT CTTCGCCTGATGAGAGGACACTCACCTTTGCTGAAAGATGGCAATTATCTTCACCTGATGGAGTAGATAC AGATGATGATTTACCAAAATCGCGAGCATCCAAAAGAACCTGTGGTGTGAATGATGATGAAAGTCCAAGC AAAATTTTTATGGTGGGAGAATCTCCACAAGTGTCTTCCAGACTTCAGAATTTGAGACTGAATAATTTAA TTCCCAGGCAACTTTTCAAGCCCACCGATAATCAAGAAACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217186 representing NM_033647
Red=Cloning site Green=Tags(s) MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDAEELCSGGVKAGSLPGCLRVSICDENT QETCKVFGRFPITGAWWRVKVQVKPVVGSRSYQYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDD VNKFLTWVKEVSNYKNLNFENLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMT ALQFPKIMEFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIAFCQC ESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASESLKFLKDIGVVTYEKSC VFPYDLYHAERAIAFSICDLMKKPPWHLCVDVEKVLASIHTTKPENSSDDALNESKPDEVRLENPVDVVD TQDNGDHIWTNGENEINAEISEVQLDQDQVEVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFK HIEQLEEREVKKACEDFEQDQNASEEWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQ VNYSFYSWTQTMMTTNKPWKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLSKLIILGDIRQLPSIEP GNLLKDLFETLKSRNCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELNISDNPTLPISIQDKTFIFV RLPEEDASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQFIAFRRQDCDLINDCCCKHYTGHLTKDHQS RLVFGIGDKICCTRNAYLSDLLPENISGSQQNNDLDASSEDFSGTLPDFAKNKRDFESNVRLCNGEIFFI TNDVTDVTFGKRRSLTINNMAGLEVTVDFKKLMKYCRIKHAWARTIHTFQGSEEQTVVYVVGKAGRQHWQ HVYTAVTRGRCRVYVIAEESQLRNAIMKNSFPRKTRLKHFLQSKLSSSGAPPADFPSPRKSSGDSGGPST PSASPLPVVTDHAMTNDVTWSEASSPDERTLTFAERWQLSSPDGVDTDDDLPKSRASKRTCGVNDDESPS KIFMVGESPQVSSRLQNLRLNNLIPRQLFKPTDNQET TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033647 |
| ORF Size | 3261 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033647.4 |
| RefSeq Size | 3368 bp |
| RefSeq ORF | 3264 bp |
| Locus ID | 92797 |
| UniProt ID | Q8NG08 |
| Gene Summary | This gene encodes a DNA-dependent ATPase which catalyzes the unwinding of DNA necessary for DNA replication, repair, recombination, and transcription. This gene is thought to function specifically during the S phase entry of the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217186 | HELB (Myc-DDK-tagged)-Human helicase (DNA) B (HELB) |
CNY 7,568.00 |
|
| RC217186L3 | Lenti ORF clone of Human helicase (DNA) B (HELB), Myc-DDK-tagged |
CNY 12,730.00 |
|
| RC217186L4 | Lenti ORF clone of Human helicase (DNA) B (HELB), mGFP tagged |
CNY 12,730.00 |
|
| SC100175 | HELB (untagged)-Human helicase (DNA) B (HELB) |
CNY 20,900.00 |
