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ARFGEF2 (NM_006420) Human Tagged ORF Clone
CAT＃: RG216885
- TrueORF®
ARFGEF2 (tGFP-tagged) - Human ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (ARFGEF2)
ORF Plasmid: DDK
"NM_006420" in other vectors (2)
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CNY 19,570.00
货期*
8周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BIG2; dJ1164I10.1; PVNH2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216885 representing NM_006420
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAGAGCCAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCCGACAAGGAGG TGAAGCGGCCCCAGCACTCCCAGCTGCGCAGGGCCTGCCAGGTGGCGCTCGATGAAATTAAAGCAGAAAT AGAAAAGCAGAGGCTTGGCACTGCTGCACCACCAAAGGCAAACTTCATTGAAGCTGACAAGTATTTTCTT CCATTCGAGCTAGCTTGCCAGTCCAAGTCCCCAAGGGTAGTCAGCACATCCCTTGACTGCTTGCAGAAAC TCATCGCATACGGGCACATCACTGGCAACGCCCCTGACAGTGGAGCCCCTGGGAAGCGGCTGATCGACAG AATTGTTGAAACCATTTGCAGTTGTTTTCAGGGCCCTCAGACTGATGAAGGGGTTCAGTTACAAATAATT AAGGCTCTTCTGACTGCAGTGACTTCCCCACACATTGAAATTCATGAGGGTACTATCCTGCAGACAGTGA GAACATGTTACAATATCTATTTGGCCAGCAAAAATCTCATCAATCAAACCACTGCCAAGGCTACCCTTAC TCAGATGCTGAACGTCATTTTCACCCGCATGGAAAACCAAGTGTTGCAGGAGGCCAGAGAACTGGAAAAA CCAATCCAGTCAAAACCCCAGTCCCCTGTGATCCAAGCTGCAGCAGTATCCCCAAAGTTCGTTCGTTTGA AGCACAGTCAGGCACAAAGCAAACCAACAACTCCCGAAAAAACAGATTTAACCAACGGTGAACATGCCAG GAGTGATTCTGGAAAAGTAAGCACAGAAAATGGAGACGCACCCAGAGAAAGAGGCTCATCACTGTCAGGG ACTGATGACGGAGCCCAGGAGGTGGTGAAGGACATCTTGGAAGATGTAGTCACATCTGCCATTAAAGAAG CAGCGGAAAAGCATGGTCTGACAGAACCTGAGAGAGTTCTAGGTGAACTGGAGTGCCAGGAATGTGCTAT TCCCCCAGGAGTTGATGAAAACTCACAGACCAACGGGATAGCCGATGACAGGCAGTCCTTGTCGTCAGCA GATAATCTGGAATCGGATGCACAAGGACATCAAGTGGCTGCCAGGTTCTCCCACGTTCTGCAGAAGGATG CCTTCCTTGTGTTCCGCTCCCTGTGCAAGCTGTCCATGAAACCCCTTGGTGAAGGCCCTCCAGACCCAAA ATCCCATGAGCTGCGTTCCAAGGTGGTTTCCCTGCAGCTGCTCCTCTCTGTGTTGCAAAATGCTGGCCCC GTATTCAGGACTCACGAGATGTTCATCAATGCAATCAAGCAATATCTCTGTGTGGCCTTGTCCAAAAACG GCGTCTCTTCAGTGCCTGATGTCTTTGAGCTCTCTCTTGCCATTTTTCTTACTCTTCTTTCAAACTTTAA AATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAGATTTTCCTGAACATTTTAGAAACATCAACA AGTTCTTTTGAGCACAGGTGGATGGTCATTCAGACTCTGACGAGGATCTGTGCAGATGCCCAGTGTGTTG TGGATATTTATGTCAACTACGACTGTGATTTAAATGCTGCTAACATTTTTGAGCGCCTTGTAAATGATTT ATCCAAAATTGCTCAGGGAAGAAGTGGACATGAGCTGGGAATGACACCTCTGCAGGAGCTCAGCCTGAGG AAGAAAGGCCTGGAGTGCCTCGTGTCCATTCTCAAGTGCATGGTGGAGTGGAGCAAAGACCTGTATGTGA ATCCCAACCACCAGACCAGCCTCGGTCAGGAGAGGCTCACGGATCAGGAAATAGGGGATGGGAAAGGCCT TGACATGGCAAGACGGTGTAGTGTGACGTCCATGGAGTCCACAGTGTCCTCGGGGACCCAGACAACTGTT CAGGATGACCCTGAGCAATTTGAGGTCATCAAGCAACAAAAAGAAATCATTGAACACGGCATCGAGCTGT TCAACAAGAAACCCAAGAGGGGGATCCAGTTTCTCCAGGAGCAGGGCATGCTGGGAACGTCAGTTGAAGA CATAGCCCAATTCCTGCACCAGGAGGAGCGCCTGGATTCCACCCAAGTAGGCGATTTTCTGGGAGATAGC GCAAGGTTCAACAAGGAGGTGATGTATGCCTACGTGGACCAACTTGACTTCTGTGAAAAAGAATTTGTCT CAGCCCTGCGGACATTCCTAGAAGGTTTCCGCCTACCTGGAGAAGCCCAAAAGATTGACCGATTAATGGA GAAGTTTGCCGCAAGATACATAGAATGCAACCAAGGGCAAACTCTGTTTGCTAGTGCTGACACTGCTTAT GTCCTAGCGTATTCAATTATTATGCTGACTACAGACTTGCACAGTCCTCAGGTAAAAAATAAAATGACGA AAGAGCAGTATATTAAAATGAATCGGGGTATCAATGATAGTAAAGATCTGCCAGAAGAGTATCTCTCAAG CATCTATGAAGAGATAGAAGGCAAGAAAATTGCAATGAAAGAAACAAAAGAGCTAACGATTGCAACCAAA TCTACTAAGCAGAATGTAGCTAGTGAAAAGCAGCGGCGGCTGCTGTACAACTTAGAGATGGAGCAAATGG CTAAAACAGCCAAAGCTCTGATGGAGGCTGTGAGCCATGCCAAAGCCCCGTTTACCAGTGCCACTCACCT GGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCACTATTGGCAGCCTACAGCATCGGACTCCAG AACTGTGATGACACTGAAGTGGCCTCCTTGTGTTTGGAAGGCATCCGATGTGCAATCCGAATCGCCTGCA TCTTTGGAATGCAGCTGGAACGAGATGCCTATGTTCAGGCTCTTGCTCGCTTCTCCCTACTCACAGCCAG CTCCAGCATCACAGAAATGAAGCAGAAAAACATCGACACCATTAAGACGCTTATCACAGTGGCTCACACC GATGGCAACTACCTTGGGAATTCCTGGCATGAGATCTTGAAATGCATCAGCCAGCTGGAGCTCGCTCAGC TGATAGGAACCGGTGTGAAGACGCGCTACCTGTCTGGATCTGGGCGTGAAAGAGAAGGGAGCCTGAAGGG CCACACATTGGCAGGAGAAGAGTTCATGGGCCTTGGCCTCGGTAATTTGGTGAGTGGCGGAGTGGATAAA AGACAGATGGCCAGCTTCCAAGAATCGGTTGGTGAGACCAGCTCGCAGAGTGTGGTTGTAGCTGTGGACA GGATTTTTACTGGGTCTACCAGACTGGATGGAAATGCAATAGTTGACTTTGTCCGCTGGCTGTGTGCTGT GTCCATGGATGAACTGGCTTCCCCCCACCATCCTCGCATGTTCAGCTTGCAGAAGATTGTGGAGATATCA TACTACAACATGAATCGGATCCGACTACAGTGGTCTCGAATATGGCATGTGATTGGAGATCACTTCAATA AGGTTGGCTGCAACCCTAATGAAGATGTGGCTATCTTTGCTGTTGACTCATTAAGGCAACTCTCCATGAA GTTTCTTGAGAAGGGTGAATTAGCCAACTTCCGTTTCCAGAAAGATTTTCTGAGGCCCTTTGAGCATATT ATGAAGAAAAACAGGTCTCCCACCATCCGGGACATGGCGATCCGCTGCATTGCCCAGATGGTGAACTCCC AGGCGGCCAACATCCGCTCAGGTTGGAAGAACATCTTTGCCGTGTTCCACCAGGCAGCCTCTGATCATGA TGGGAACATTGTGGAGCTGGCCTTCCAGACCACTTGCCACATTGTCACAACTATTTTCCAGCACCATTTT CCTGCAGCCATCGATTCCTTTCAGGATGCTGTGAAGTGCTTATCAGAGTTCGCCTGCAACGCCGCTTTCC CTGACACGAGCATGGAAGCGATTCGGCTCATCCGCTTCTGTGGCAAATACGTCTCTGAGAGGCCTCGGGT GCTACAAGAATACACAAGTGATGACATGAATGTAGCTCCTGGTGACAGAGTCTGGGTCCGAGGCTGGTTC CCCATCTTATTCGAACTCTCCTGCATCATTAATAGATGCAAGTTAGATGTACGAACAAGGGGACTCACAG TCATGTTTGAGATCATGAAGAGCTATGGCCACACCTTTGAAAAGCACTGGTGGCAGGACCTGTTCAGAAT CGTGTTTCGGATTTTTGACAATATGAAACTCCCTGAGCAACTGTCAGAGAAATCTGAGTGGATGACAACA ACCTGCAATCACGCACTTTATGCTATTTGTGATGTTTTTACCCAGTTTTATGAAGCTTTGAATGAAGTTC TTCTTTCTGATGTATTTGCACAATTGCAGTGGTGTGTCAAACAAGATAATGAACAGTTGGCGCGATCAGG TACAAATTGCTTAGAAAACTTAGTAATATCCAATGGAGAGAAATTCAGTCCTGAAGTCTGGGATGAAACC TGCAACTGTATGTTGGATATTTTCAAAACAACCATCCCACATGTTTTGCTGACATGGAGACCTGTAGGAA TGGAGGAAGATTCATCAGAAAAGCATTTGGATGTGGATCTGGACCGCCAGTCTTTAAGCAGCATAGATAA AAATCCCTCTGAGAGGGGACAGAGCCAGCTCTCTAACCCAACAGATGACAGCTGGAAGGGTAGACCATAC GCAAATCAGAAACTGTTTGCCAGCCTCCTCATCAAGTGTGTGGTCCAGTTGGAATTGATACAGACCATTG ACAACATTGTGTTCTACCCTGCGACGAGCAAAAAGGAGGATGCAGAGCACATGGTTGCCGCCCAGCAAGA CACGCTGGATGCAGATATCCACATAGAGACGGAGGATCAGGGCATGTATAAGTACATGTCTTCCCAGCAC CTCTTCAAGCTGTTGGACTGTTTGCAGGAATCCCATTCATTCTCAAAGGCCTTCAACTCCAATTACGAGC AGCGGACTGTCCTGTGGCGAGCAGGTTTTAAGGGCAAGTCTAAACCCAATCTTCTAAAACAAGAAACCAG CAGCCTGGCCTGTTGTTTGAGGATCCTGTTTCGAATGTATGTTGATGAGAACCGCAGGGATTCCTGGGAA GAAATACAGCAGAGACTTTTAACTGTTTGCAGTGAAGCTCTTGCCTATTTCATCACTGTGAATTCTGAGA GCCATCGGGAGGCCTGGACAAGTCTCTTGTTGTTACTTCTAACTAAAACCCTCAAAATAAATGATGAAAA GTTCAAAGCACATGCTTCAATGTACTACCCCTACTTGTGTGAAATTATGCAGTTTGACCTGATCCCTGAG CTCCGAGCAGTTCTGCGGAAGTTCTTCCTACGGATAGGTGTTGTGTATAAGATATGGATACCAGAAGAGC CATCACAGGTACCAGCAGCACTGTCACCAGTGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216885 representing NM_006420
Red=Cloning site Green=Tags(s) MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFL PFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQII KALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEK PIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSG TDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSA DNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGP VFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETST SSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLR KKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTV QDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDS ARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAY VLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATK STKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQ NCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHT DGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDK RQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEIS YYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHI MKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHF PAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWF PILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTT TCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDET CNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPY ANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQH LFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWE EIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPE LRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006420 |
| ORF Size | 5355 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006420.3 |
| RefSeq Size | 9004 bp |
| RefSeq ORF | 5358 bp |
| Locus ID | 10564 |
| UniProt ID | Q9Y6D5 |
| Gene Summary | ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216885 | ARFGEF2 (Myc-DDK-tagged)-Human ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (ARFGEF2) |
CNY 17,770.00 |
|
| SC303772 | ARFGEF2 (untagged)-Human ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (ARFGEF2) |
CNY 34,390.00 |
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