NEMF (NM_004713) Human Tagged ORF Clone
CAT#: RG216806
- TrueORF®
NEMF (tGFP-tagged) - Human nuclear export mediator factor (NEMF)
ORF Plasmid: DDK
"NM_004713" in other vectors (4)
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CNY 11,780.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | IDDSAPN; NY-CO-1; SDCCAG1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216806 representing NM_004713
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGCCGCTTTAGCACCATTGACCTCCGCGCCGTACTCGCGGAGCTGAATGCTAGCTTGCTAGGAA TGAGAGTAAACAATGTTTATGATGTGGATAATAAGACATACCTTATTCGTCTTCAAAAACCGGACTTTAA AGCTACACTTTTACTTGAATCTGGCATACGAATTCATACAACAGAATTTGAGTGGCCTAAGAATATGATG CCGTCTAGTTTTGCCATGAAGTGCCGAAAACATTTGAAGAGTCGGAGATTAGTCAGTGCAAAACAGCTTG GTGTGGATAGAATTGTAGATTTTCAATTTGGAAGTGATGAAGCTGCTTACCATTTAATCATTGAGCTCTA TGATAGGGGGAACATTGTTCTTACAGATTATGAGTACGTAATTTTAAATATTCTAAGGTTTCGAACTGAT GAGGCAGATGATGTTAAATTTGCTGTTCGTGAACGCTATCCACTTGATCATGCTAGAGCTGCTGAACCTT TGCTTACTTTGGAAAGGTTGACTGAAATAGTAGCCAGCGCACCTAAGGGTGAACTACTGAAGAGGGTGCT TAACCCATTACTTCCCTATGGACCAGCTCTCATTGAACACTGTCTTTTAGAAAATGGATTCTCGGGTAAT GTCAAAGTGGATGAAAAACTTGAAACTAAAGATATTGAAAAAGTACTTGTTTCTCTGCAGAAAGCAGAAG ACTATATGAAAACAACATCCAACTTCAGTGGGAAGGGATATATCATTCAGAAAAGAGAAATAAAACCATG CTTGGAAGCAGATAAACCAGTTGAAGACATACTGACGTATGAGGAATTTCATCCTTTCTTGTTTTCTCAA CATTCACAATGTCCATATATAGAATTTGAATCATTTGACAAGGCGGTGGATGAATTTTATTCCAAGATAG AAGGCCAGAAAATTGACTTAAAAGCTTTACAACAGGAAAAGCAAGCATTGAAGAAATTAGATAATGTTCG AAAGGATCACGAAAACAGATTGGAAGCTCTTCAGCAGGCTCAGGAAATAGACAAACTGAAAGGAGAGCTC ATAGAAATGAACCTACAAATAGTTGACAGAGCCATTCAGGTAGTTCGAAGTGCTTTAGCTAACCAGATAG ATTGGACAGAAATTGGGTTAATTGTGAAAGAAGCCCAGGCTCAAGGAGACCCTGTTGCAAGTGCAATCAA AGAATTAAAACTACAAACAAACCATGTTACAATGCTGCTAAGAAATCCATACTTGTTATCAGAGGAGGAA GATGATGATGTTGATGGTGACGTCAATGTTGAGAAAAATGAAACTGAACCACCAAAAGGAAAAAAGAAAA AACAAAAGAATAAACAGCTGCAGAAGCCTCAGAAAAATAAGCCCTTACTTGTAGATGTTGATCTCAGCTT GTCAGCATATGCCAATGCCAAAAAGTATTATGATCACAAGAGATATGCTGCTAAGAAAACACAAAAGACT GTTGAAGCTGCTGAGAAGGCATTCAAGTCAGCAGAAAAGAAAACAAAGCAAACATTAAAAGAAGTTCAGA CTGTTACCTCTATTCAAAAAGCAAGAAAAGTATATTGGTTTGAGAAATTTCTGTGGTTCATTAGCTCAGA GAACTATCTAATTATAGGTGGACGAGATCAGCAACAGAATGAAATAATTGTGAAAAGATACTTGACACCA GGAGACATTTATGTACATGCTGATCTTCATGGAGCTACTAGCTGTGTAATTAAGAATCCAACAGGAGAAC CCATCCCCCCACGGACCTTGACTGAAGCTGGCACAATGGCACTTTGCTACAGTGCTGCTTGGGATGCACG AGTTATCACTAGTGCTTGGTGGGTGTACCATCATCAGGTATCTAAAACAGCACCAACTGGAGAATATTTG ACAACAGGAAGCTTCATGATAAGAGGAAAAAAGAATTTTCTTCCTCCCTCATATCTAATGATGGGGTTTA GCTTCCTTTTTAAGGTAGATGAGTCTTGTGTTTGGAGACATCAGGGTGAACGAAAAGTCAGAGTACAGGA TGAAGACATGGAGACACTGGCAAGTTGTACAAGTGAACTCATATCAGAAGAAATGGAACAATTAGATGGA GGTGACACGAGCAGTGATGAGGATAAAGAAGAACATGAAACTCCTGTGGAAGTAGAACTCATGACTCAGG TTGACCAAGAGGATATCACTCTTCAGAGTGGCAGAGATGAACTAAATGAGGAGCTCATTCAGGAAGAAAG CTCTGAAGACGAAGGAGAATATGAAGAGGTTAGAAAAGATCAGGATTCTGTTGGTGAAATGAAGGATGAA GGGGAAGAGACATTAAATTATCCTGATACTACCATTGACTTGTCTCACCTTCAACCCCAAAGGTCCATCC AGAAATTGGCTTCAAAAGAGGAATCTTCTAATTCTAGTGACAGTAAATCACAGAGCCGGAGACATTTGTC AGCCAAGGAAAGAAGGGAAATGAAAAAGAAAAAACTTCCAAGTGACTCAGGAGATTTAGAAGCGTTAGAG GGAAAGGATAAAGAAAAAGAAAGTACTGTACACATTGAAACTCATCAGAACACAAGCAAAAATGTTGCGG CTGTGCAGCCAATGAAACGAGGACAAAAGAGTAAAATGAAAAAAATGAAAGAAAAATACAAAGACCAGGA TGAAGAAGACCGTGAACTTATCATGAAGTTGCTGGGGTCTGCAGGTTCAAACAAAGAAGAAAAAGGGAAG AAGGGGAAGAAAGGAAAAACAAAGGACGAACCTGTGAAGAAACAGCCCCAGAAACCTAGAGGTGGACAGA GGGTCTCTGACAACATTAAGAAAGAAACTCCGTTCCTTGAGGTTATAACTCATGAGTTACAAGACTTTGC TGTAGATGATCCACATGATGACAAGGAAGAGCAAGATCTGGATCAACAGGGAAATGAGGAAAACCTATTT GATTCTTTGACAGGCCAGCCACATCCTGAAGATGTACTACTGTTTGCCATTCCAATATGTGCCCCTTACA CCACCATGACAAACTACAAATATAAAGTGAAACTTACTCCTGGAGTGCAGAAAAAGGGAAAAGCTGCAAA AACAGCCTTGAATAGTTTCATGCATTCCAAAGAAGCAACAGCAAGAGAAAAAGACTTATTCCGCAGCGTA AAGGACACAGATTTATCAAGAAACATTCCTGGCAAAGTGAAAGTGTCTGCACCCAATCTTCTGAACGTAA AAAGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216806 representing NM_004713
Red=Cloning site Green=Tags(s) MKSRFSTIDLRAVLAELNASLLGMRVNNVYDVDNKTYLIRLQKPDFKATLLLESGIRIHTTEFEWPKNMM PSSFAMKCRKHLKSRRLVSAKQLGVDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDYEYVILNILRFRTD EADDVKFAVRERYPLDHARAAEPLLTLERLTEIVASAPKGELLKRVLNPLLPYGPALIEHCLLENGFSGN VKVDEKLETKDIEKVLVSLQKAEDYMKTTSNFSGKGYIIQKREIKPCLEADKPVEDILTYEEFHPFLFSQ HSQCPYIEFESFDKAVDEFYSKIEGQKIDLKALQQEKQALKKLDNVRKDHENRLEALQQAQEIDKLKGEL IEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDPVASAIKELKLQTNHVTMLLRNPYLLSEEE DDDVDGDVNVEKNETEPPKGKKKKQKNKQLQKPQKNKPLLVDVDLSLSAYANAKKYYDHKRYAAKKTQKT VEAAEKAFKSAEKKTKQTLKEVQTVTSIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEIIVKRYLTP GDIYVHADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYL TTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHQGERKVRVQDEDMETLASCTSELISEEMEQLDG GDTSSDEDKEEHETPVEVELMTQVDQEDITLQSGRDELNEELIQEESSEDEGEYEEVRKDQDSVGEMKDE GEETLNYPDTTIDLSHLQPQRSIQKLASKEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALE GKDKEKESTVHIETHQNTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGK KGKKGKTKDEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQQGNEENLF DSLTGQPHPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSV KDTDLSRNIPGKVKVSAPNLLNVKRK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004713 |
ORF Size | 3228 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004713.5, NP_004704.2 |
RefSeq Size | 4136 bp |
RefSeq ORF | 3231 bp |
Locus ID | 9147 |
UniProt ID | O60524 |
Gene Summary | This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216806 | NEMF (Myc-DDK-tagged)-Human nuclear export mediator factor (NEMF) |
CNY 7,496.00 |
|
RC216806L3 | Lenti ORF clone of Human nuclear export mediator factor (NEMF), Myc-DDK-tagged |
CNY 12,640.00 |
|
RC216806L4 | Lenti ORF clone of Human nuclear export mediator factor (NEMF), mGFP tagged |
CNY 12,640.00 |
|
SC315149 | NEMF (untagged)-Human nuclear export mediator factor (NEMF) |
CNY 7,496.00 |