TAF1 (NM_138923) Human Tagged ORF Clone
CAT#: RG216684
- TrueORF®
TAF1 (tGFP-tagged) - Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 2
ORF Plasmid: DDK
"NM_138923" in other vectors (2)
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CNY 20,620.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216684 representing NM_138923
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCCGGCTGCGATTTGCTGCTGCGGACAGCAGCTACCATCACTGCTGCCGCCATCATGTCAGACA CGGACAGCGACGAAGATTCCGCTGGAGGCGGCCCATTTTCTTTAGCGGGTTTCCTTTTCGGCAACATCAA TGGAGCCGGGCAGCTGGAGGGGGAAAGCGTCTTGGATGATGAATGTAAGAAGCACTTGGCAGGCTTGGGG GCTTTGGGGCTGGGCAGCCTGATCACTGAACTCACGGCAAATGAAGAATTGACCGGGACTGACGGTGCCT TGGTAAATGATGAAGGGTGGGTTAGGAGTACAGAAGATGCTGTGGACTATTCAGACATCAATGAGGTGGC AGAAGATGAAAGCCGAAGATACCAGCAGACGATGGGGAGCTTGCAGCCCCTTTGCCACTCAGATTATGAT GAAGATGACTATGATGCTGATTGTGAAGACATTGATTGCAAGTTGATGCCTCCTCCACCTCCACCCCCGG GACCAATGAAGAAGGATAAGGACCAGGATTCTATTACTGGTGAGAAAGTGGACTTCAGTAGTTCCTCTGA CTCAGAATCTGAGATGGGACCTCAGGAAGCAACACAGGCAGAATCTGAAGATGGAAAGCTGACCCTTCCA TTGGCTGGGATTATGCAGCATGATGCCACCAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTC GACCTGGAAAGGTGTTACGTTTTCTACGTCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGGCGGAG TGCTCGGAGAAAGAGGAAGAAGAAGCACCGTGAGCTGATACAGGAAGAGCAGATCCAGGAGGTGGAGTGC TCAGTAGAATCAGAAGTCAGCCAGAAGTCTTTGTGGAACTACGACTACGCTCCACCACCACCTCCAGAGC AGTGTCTCTCTGATGATGAAATCACGATGATGGCTCCTGTGGAGTCCAAATTTTCCCAATCAACTGGAGA TATAGATAAAGTGACAGATACCAAACCAAGAGTGGCTGAGTGGCGTTATGGGCCTGCCCGACTGTGGTAT GATATGCTGGGTGTCCCTGAAGATGGCAGTGGGTTTGACTATGGCTTCAAACTGAGAAAGACAGAACATG AACCTGTGATAAAATCTAGAATGATAGAGGAATTTAGGAAACTTGAGGAAAACAATGGCACTGATCTTCT GGCTGATGAAAACTTCCTGATGGTGACACAGCTGCATTGGGAGGATGATATCATCTGGGATGGGGAGGAT GTCAAACACAAAGGGACAAAACCTCAGCGTGCAAGCCTGGCAGGCTGGCTTCCTTCTAGCATGACTAGGA ATGCGATGGCTTACAATGTTCAGCAAGGTTTTGCAGCCACTCTTGATGATGACAAACCTTGGTACTCCAT TTTTCCCATTGACAATGAGGATCTGGTATATGGACGCTGGGAGGACAATATCATTTGGGATGCTCAGGCC ATGCCCCGGCTGTTGGAACCTCCTGTTTTGACACTTGATCCCAATGATGAGAACCTCATTTTGGAAATTC CTGATGAGAAGGAAGAGGCCACCTCTAACTCCCCCTCCAAGGAGAGTAAGAAGGAATCATCTCTGAAGAA GAGTCGAATTCTCTTAGGGAAAACAGGAGTCATCAAGGAGGAACCACAGCAGAACATGTCTCAGCCAGAA GTGAAAGATCCATGGAATCTCTCCAATGATGAGTATTATTATCCCAAGCAACAGGGTCTTCGAGGCACCT TTGGAGGGAATATTATCCAGCATTCAATTCCTGCTGTGGAATTACGGCAGCCCTTCTTTCCCACCCACAT GGGGCCCATCAAACTCCGGCAGTTCCATCGCCCACCTCTGAAAAAGTACTCATTTGGTGCACTTTCTCAG CCAGGTCCCCACTCAGTCCAACCTTTGCTAAAGCACATCAAAAAAAAGGCCAAGATGAGAGAACAAGAGA GGCAAGCTTCAGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGACCTCACAGGCAAAGATGGTGA TCTTATTCTTGCAGAATATAGTGAGGAAAATGGACCCTTAATGATGCAGGTTGGCATGGCAACCAAGATA AAGAACTATTATAAACGGAAACCTGGAAAAGATCCTGGAGCACCAGATTGTAAATATGGGGAAACTGTTT ACTGCCATACATCTCCTTTCCTGGGTTCTCTCCATCCTGGCCAATTGCTGCAAGCATTTGAGAACAACCT TTTTCGTGCTCCAATTTATCTTCATAAGATGCCAGAAACTGATTTCTTGATCATTCGGACAAGACAGGGT TACTATATTCGGGAATTAGTGGATATTTTTGTGGTTGGCCAGCAGTGTCCCTTGTTTGAAGTTCCTGGGC CTAACTCCAAAAGGGCCAATACGCATATTCGAGACTTTCTACAGGTTTTTATTTACCGCCTTTTCTGGAA AAGTAAAGATCGGCCACGGAGGATACGAATGGAAGATATAAAAAAAGCCTTTCCTTCCCATTCAGAAAGC AGCATCCGGAAGAGGCTAAAGCTCTGCGCTGACTTCAAACGCACAGGGATGGACTCAAACTGGTGGGTGC TTAAGTCTGATTTTCGTTTACCAACGGAAGAAGAGATCAGAGCTATGGTGTCACCAGAGCAGTGCTGTGC TTATTATAGCATGATAGCTGCAGAGCAACGACTGAAGGATGCTGGCTATGGTGAGAAATCCTTTTTTGCT CCAGAAGAAGAAAATGAGGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGCACTGCCCCTTGGAACA CCACAAGGGCCTTCATTGCTGCCATGAAGGGCAAGTGTCTGCTAGAGGTGACTGGGGTGGCAGATCCCAC GGGGTGTGGTGAAGGATTCTCCTATGTGAAGATTCCAAACAAACCAACACAGCAGAAGGATGATAAAGAA CCGCAGCCAGTGAAGAAGACAGTGACAGGAACAGATGCAGACCTTCGTCGCCTTTCCCTGAAAAATGCCA AGCAACTTCTACGTAAATTTGGTGTGCCTGAGGAAGAGATTAAAAAGTTGTCCCGCTGGGAAGTGATTGA TGTGGTGCGCACAATGTCAACAGAACAGGCTCGTTCTGGAGAGGGGCCCATGAGTAAATTTGCCCGTGGA TCAAGGTTTTCTGTGGCTGAGCATCAAGAGCGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGA ACAAGGTTCTGTCATCAACTGAAGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGATAGTGACTT TGAAGAAATGGGAAAGAACATTGAGAACATGTTGCAGAACAAGAAAACCAGCTCTCAGCTTTCACGTGAA CGGGAGGAACAGGAGCGGAAGGAACTACAGCGAATGCTACTGGCAGCAGGCTCAGCAGCATCCGGAAACA ATCACAGAGATGATGACACAGCTTCCGTGACTAGCCTTAACTCTTCTGCCACTGGACGCTGTCTCAAGAT TTATCGCACGTTTCGAGATGAAGAGGGGAAAGAGTATGTTCGCTGTGAGACAGTCCGAAAACCAGCTGTC ATTGATGCCTATGTGCGCATACGGACTACAAAAGATGAGGAATTCATTCGAAAATTTGCCCTTTTTGATG AACAACATCGGGAAGAGATGCGAAAAGAACGGCGGAGGATTCAAGAGCAACTGAGGCGGCTTAAGAGGAA CCAGGAAAAGGAGAAGCTTAAGGGTCCTCCTGAGAAGAAGCCCAAGAAAATGAAGGAGCGTCCTGACCTA AAACTGAAATGTGGGGCATGTGGTGCCATTGGACACATGAGGACTAACAAATTCTGCCCCCTCTATTATC AAACAAATGCGCCACCTTCCAACCCTGTTGCCATGACAGAAGAACAGGAGGAGGAGTTGGAAAAGACAGT CATTCATAATGATAATGAAGAACTTATCAAGGTTGAAGGGACCAAAATTGTCTTGGGGAAACAGCTAATT GAGAGTGCGGATGAGGTTCGCAGAAAATCTCTGGTTCTCAAGTTTCCTAAACAGCAGCTTCCTCCAAAGA AGAAACGGCGAGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAGTCCATCCACCGGCG CCGCACAGACCCTATGGTGACGCTGTCGTCCATCTTGGAGTCTATCATCAATGACATGAGAGATCTTCCA AATACATACCCTTTCCACACTCCAGTCAATGCAAAGGTTGTAAAGGACTACTACAAAATCATCACTCGGC CAATGGACCTACAAACACTCCGCGAAAACGTGCGTAAACGCCTCTACCCATCTCGGGAAGAGTTCAGAGA GCATCTGGAGCTAATTGTGAAAAATAGTGCAACCTACAATGGGCCAAAACACTCATTGACTCAGATCTCT CAATCCATGCTGGATCTCTGTGATGAAAAACTCAAAGAGAAAGAAGACAAATTAGCTCGCTTAGAGAAAG CTATCAACCCCTTGCTGGATGATGATGACCAAGTGGCGTTTTCTTTCATTCTGGACAACATTGTCACCCA GAAAATGATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTAT TACAAAGTGATTGTCAATCCAATGGATTTAGAGACCATACGTAAGAACATCTCCAAGCACAAGTATCAGA GTCGGGAGAGCTTTCTGGATGATGTAAACCTTATTCTGGCCAACAGTGTTAAGTATAATGGACCTGAGAG TCAGTATACTAAGACTGCCCAGGAGATTGTGAACGTCTGTTACCAGACATTGACTGAGTATGATGAACAT TTGACTCAACTTGAGAAGGATATTTGTACTGCTAAAGAAGCAGCTTTGGAGGAAGCAGAATTAGAAAGCC TGGACCCAATGACCCCAGGGCCCTACACGCCTCAGCCTCCTGATTTGTATGATACCAACACATCCCTCAG TATGTCTCGAGATGCCTCTGTATTTCAAGATGAGAGCAATATGTCTGTCTTGGATATTCCCAGTGCCACT CCAGAAAAGCAGGTAACACAGGAAGGTGAAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTG TACAACAGCCTCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCTGAAGGAGAAGATGATGAGGAAGA TGCTGGGAGTGATGAAGAAGGAGACAATCCTTTCTCTGCTATCCAGCTGAGTGAAAGTGGAAGTGACTCT GATGTGGGATCTGGTGGAATAAGACCCAAACAACCCCGCATGCTTCAGGAGAACACAAGGATGGACATGG AAAATGAAGAAAGCATGATGTCCTATGAGGGAGACGGTGGGGAGGCTTCCCATGGTTTGGAGGATAGCAA CATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAAGACACAAGCTTCAGCAGCATC GGTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAGGAGGAAGAGCAGCGCTCTGGGCCGAGCGTAC TAAGCCAGGTCCACCTGTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCTGGGGACAGTGA CTTGGACTCTGATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216684 representing NM_138923
Red=Cloning site Green=Tags(s) MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLG ALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHSDYD EDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGEKVDFSSSSDSESEMGPQEATQAESEDGKLTLP LAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVEC SVESEVSQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWY DMLGVPEDGSGFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLADENFLMVTQLHWEDDIIWDGED VKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEDLVYGRWEDNIIWDAQA MPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPE VKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQ PGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKI KNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQG YYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSES SIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFA PEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKE PQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARG SRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSRE REEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAV IDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDL KLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLI ESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLP NTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQIS QSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDY YKVIVNPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEH LTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPSAT PEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDS DVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSI GGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138923 |
ORF Size | 5616 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138923.3 |
RefSeq Size | 7646 bp |
RefSeq ORF | 5559 bp |
Locus ID | 6872 |
UniProt ID | P21675 |
Protein Families | Protein Kinase |
Protein Pathways | Basal transcription factors |
Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216684 | TAF1 (Myc-DDK-tagged)-Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 2 |
CNY 18,720.00 |
|
SC306088 | TAF1 (untagged)-Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 2 |
CNY 36,010.00 |