ELF5 (NM_198381) Human Tagged ORF Clone
CAT#: RG216682
- TrueORF®
ELF5 (tGFP-tagged) - Human E74-like factor 5 (ets domain transcription factor) (ELF5), transcript variant 1
ORF Plasmid: DDK
"NM_198381" in other vectors (4)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ESE2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216682 representing NM_198381
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATCTCTGCCTCACTCCCACAGGGTAATGTTGGACTCGGTGACACACAGCACCTTCCTGCCTAATG CATCCTTCTGCGATCCCCTGATGTCGTGGACTGATCTGTTCAGCAATGAAGAGTACTACCCTGCCTTTGA GCATCAGACAGCCTGTGACTCATACTGGACATCAGTCCACCCTGAATACTGGACTAAGCGCCATGTGTGG GAGTGGCTCCAGTTCTGCTGCGACCAGTACAAGTTGGACACCAATTGCATCTCCTTCTGCAACTTCAACA TCAGTGGCCTGCAGCTGTGCAGCATGACACAGGAGGAGTTCGTCGAGGCAGCTGGCCTCTGCGGCGAGTA CCTGTACTTCATCCTCCAGAACATCCGCACACAAGGTTACTCCTTTTTTAATGACGCTGAAGAAAGCAAG GCCACCATCAAAGACTATGCTGATTCCAACTGCTTGAAAACAAGTGGCATCAAAAGTCAAGACTGTCACA GTCATAGTAGAACAAGCCTCCAAAGTTCTCATCTATGGGAATTTGTACGAGACCTGCTTCTATCTCCTGA AGAAAACTGTGGCATTCTGGAATGGGAAGATAGGGAACAAGGAATTTTTCGGGTGGTTAAATCGGAAGCC CTGGCAAAGATGTGGGGACAAAGGAAGAAAAATGACAGAATGACATATGAAAAGTTGAGCAGAGCCCTGA GATACTACTATAAAACAGGAATTTTGGAGCGGGTTGACCGAAGGTTAGTGTACAAATTTGGAAAAAATGC ACACGGGTGGCAGGAAGACAAGCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216682 representing NM_198381
Red=Cloning site Green=Tags(s) MPSLPHSHRVMLDSVTHSTFLPNASFCDPLMSWTDLFSNEEYYPAFEHQTACDSYWTSVHPEYWTKRHVW EWLQFCCDQYKLDTNCISFCNFNISGLQLCSMTQEEFVEAAGLCGEYLYFILQNIRTQGYSFFNDAEESK ATIKDYADSNCLKTSGIKSQDCHSHSRTSLQSSHLWEFVRDLLLSPEENCGILEWEDREQGIFRVVKSEA LAKMWGQRKKNDRMTYEKLSRALRYYYKTGILERVDRRLVYKFGKNAHGWQEDKL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_198381 |
ORF Size | 795 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_198381.2 |
RefSeq Size | 2466 bp |
RefSeq ORF | 798 bp |
Locus ID | 2001 |
UniProt ID | Q9UKW6 |
Protein Families | Transcription Factors |
Gene Summary | The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216682 | ELF5 (Myc-DDK-tagged)-Human E74-like factor 5 (ets domain transcription factor) (ELF5), transcript variant 1 |
CNY 3,990.00 |
|
RC216682L3 | Lenti-ORF clone of ELF5 (Myc-DDK-tagged)-Human E74-like factor 5 (ets domain transcription factor) (ELF5), transcript variant 1 |
CNY 5,890.00 |
|
RC216682L4 | Lenti-ORF clone of ELF5 (mGFP-tagged)-Human E74-like factor 5 (ets domain transcription factor) (ELF5), transcript variant 1 |
CNY 5,890.00 |
|
SC307675 | ELF5 (untagged)-Human E74-like factor 5 (ets domain transcription factor) (ELF5), transcript variant 1 |
CNY 3,990.00 |