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MYO10 (NM_012334) Human Tagged ORF Clone
CAT＃: RG216606
- TrueORF®
MYO10 (tGFP-tagged) - Human myosin X (MYO10)
ORF Plasmid: DDK
"NM_012334" in other vectors (2)
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CNY 20,900.00
货期*
13周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216606 representing NM_012334
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAACTTCTTCACCGAGGGAACACGGGTCTGGCTGAGAGAAAATGGCCAGCATTTTCCAAGTACTG TAAATTCCTGTGCAGAAGGCATCGTCGTCTTCCGGACAGACTATGGTCAGGTATTCACTTACAAGCAGAG CACAATTACCCACCAGAAGGTGACTGCTATGCACCCCACGAACGAGGAGGGCGTGGATGACATGGCGTCC TTGACAGAGCTCCATGGCGGCTCCATCATGTATAACTTATTCCAGCGGTATAAGAGAAATCAAATATATA CCTACATCGGCTCCATCCTGGCCTCCGTGAACCCCTACCAGCCCATCGCCGGGCTGTACGAGCCTGCCAC CATGGAGCAGTACAGCCGGCGCCACCTGGGCGAGCTGCCCCCGCACATCTTCGCCATCGCCAACGAGTGC TACCGCTGCCTGTGGAAGCGCTACGACAACCAGTGCATCCTCATCAGTGGTGAAAGTGGGGCAGGTAAAA CCGAAAGCACTAAATTGATCCTCAAGTTTCTGTCAGTCATCAGTCAACAGTCTTTGGAATTGTCCTTAAA GGAGAAGACATCCTGTGTTGAACGAGCTATTCTTGAAAGCAGCCCCATCATGGAAGCTTTCGGCAATGCG AAGACCGTGTACAACAACAACTCTAGTCGCTTTGGGAAGTTTGTTCAGCTGAACATCTGTCAGAAAGGAA ATATTCAGGGCGGGAGAATTGTAGATTATTTATTAGAAAAAAACCGAGTAGTAAGGCAAAATCCCGGGGA AAGGAATTATCACATATTTTATGCACTGCTGGCAGGGCTGGAACATGAAGAAAGAGAAGAATTTTATTTA TCTACGCCAGAAAACTACCACTACTTGAATCAGTCTGGATGTGTAGAAGACAAGACAATCAGTGACCAGG AATCCTTTAGGGAAGTTATTACGGCAATGGACGTGATGCAGTTCAGCAAGGAGGAAGTTCGGGAAGTGTC GAGGCTGCTTGCTGGTATACTGCATCTTGGGAACATAGAATTTATCACTGCTGGTGGGGCACAGGTTTCC TTCAAAACAGCTTTGGGCAGATCTGCGGAGTTACTTGGGCTGGACCCAACACAGCTCACAGATGCTTTGA CCCAGAGATCAATGTTCCTCAGGGGAGAAGAGATCCTCACGCCTCTCAATGTTCAACAGGCAGTAGACAG CAGGGACTCCCTGGCCATGGCTCTGTATGCGTGCTGCTTTGAGTGGGTAATCAAGAAGATCAACAGCAGG ATCAAAGGCAATGAGGACTTCAAGTCTATTGGCATCCTCGACATCTTTGGATTTGAAAACTTTGAGGTTA ATCACTTTGAACAGTTCAATATAAACTATGCAAACGAGAAACTTCAGGAGTACTTCAACAAGCATATTTT TTCTTTAGAACAACTAGAATATAGCCGGGAAGGATTAGTGTGGGAAGATATTGACTGGATAGACAATGGA GAATGCCTGGACTTGATTGAGAAGAAACTTGGCCTCCTAGCCCTTATCAATGAAGAAAGCCATTTTCCTC AAGCCACAGACAGCACCTTATTGGAGAAGCTACACAGTCAGCATGCGAATAACCACTTTTATGTGAAGCC CAGAGTTGCAGTTAACAATTTTGGAGTGAAGCACTATGCTGGAGAGGTGCAATATGATGTCCGAGGTATC TTGGAGAAGAACAGAGATACATTTCGAGATGACCTTCTCAATTTGCTAAGAGAAAGCCGATTTGACTTTA TCTACGATCTTTTTGAACATGTTTCAAGCCGCAACAACCAGGATACCTTGAAATGTGGAAGCAAACATCG GCGGCCTACAGTCAGCTCACAGTTCAAGGACTCACTGCATTCCTTAATGGCAACGCTAAGCTCCTCTAAT CCTTTCTTTGTTCGCTGTATCAAGCCAAACATGCAGAAGATGCCAGACCAGTTTGACCAGGCGGTTGTGC TGAACCAGCTGCGGTACTCAGGGATGCTGGAGACTGTGAGAATCCGCAAAGCTGGGTATGCGGTCCGAAG ACCCTTTCAGGACTTTTACAAAAGGTATAAAGTGCTGATGAGGAATCTGGCTCTGCCTGAGGACGTCCGA GGGAAGTGCACGAGCCTGCTGCAGCTCTATGATGCCTCCAACAGCGAGTGGCAGCTGGGGAAGACCAAGG TCTTTCTTCGAGAATCCTTGGAACAGAAACTGGAGAAGCGGAGGGAAGAGGAAGTGAGCCACGCGGCCAT GGTGATTCGGGCCCATGTCTTGGGCTTCTTAGCACGAAAACAATACAGAAAGGTCCTTTATTGTGTGGTG ATAATACAGAAGAATTACAGAGCATTCCTTCTGAGGAGGAGATTTTTGCACCTGAAAAAGGCAGCCATAG TTTTCCAGAAGCAACTCAGAGGTCAGATTGCTCGGAGAGTTTACAGACAATTGCTGGCAGAGAAAAGGGA GCAAGAAGAAAAGAAGAAACAGGAAGAGGAAGAAAAGAAGAAACGGGAGGAAGAAGAAAGAGAAAGAGAG AGAGAGCGAAGAGAAGCCGAGCTCCGCGCCCAGCAGGAAGAAGAAACGAGGAAGCAGCAAGAACTCGAAG CCTTGCAGAAGAGCCAGAAGGAAGCTGAACTGACCCGTGAACTGGAGAAACAGAAGGAAAATAAGCAGGT GGAAGAGATCCTCCGTCTGGAGAAAGAAATCGAGGACCTGCAGCGCATGAAGGAGCAGCAGGAGCTGTCG CTGACCGAGGCTTCCCTGCAGAAGCTGCAGGAGCGGCGGGACCAGGAGCTCCGCAGGCTGGAGGAGGAAG CGTGCAGGGCGGCCCAGGAGTTCCTCGAGTCCCTCAATTTCGACGAGATCGACGAGTGTGTCCGGAATAT CGAGCGGTCCCTGTCGGTGGGAAGCGAATTTTCCAGCGAGCTGGCTGAGAGCGCATGCGAGGAGAAGCCC AACTTCAACTTCAGCCAGCCCTACCCAGAGGAGGAGGTCGATGAGGGCTTCGAAGCCGACGACGACGCCT TCAAGGACTCCCCCAACCCCAGCGAGCACGGCCACTCAGACCAGCGAACAAGTGGCATCCGGACCAGCGA TGACTCTTCAGAGGAGGACCCATACATGAACGACACGGTGGTGCCCACCAGCCCCAGTGCGGACAGCACG GTGCTGCTCGCCCCATCAGTGCAGGACTCCGGGAGCCTACACAACTCCTCCAGCGGCGAGTCCACCTACT GCATGCCCCAGAACGCTGGGGACTTGCCCTCCCCAGACGGCGACTACGACTACGACCAGGATGACTATGA GGACGGTGCCATCACTTCCGGCAGCAGCGTGACCTTCTCCAACTCCTACGGCAGCCAGTGGTCCCCCGAC TACCGCTGCTCTGTGGGGACCTACAACAGCTCGGGTGCCTACCGGTTCAGCTCTGAGGGGGCGCAGTCCT CGTTTGAAGATAGTGAAGAGGACTTTGATTCCAGGTTTGATACAGATGATGAGCTTTCATACCGGCGTGA CTCTGTGTACAGCTGTGTCACTCTGCCGTATTTCCACAGCTTTCTGTACATGAAAGGTGGCCTGATGAAC TCTTGGAAACGCCGCTGGTGCGTCCTCAAGGATGAAACCTTCTTGTGGTTCCGCTCCAAGCAGGAGGCCC TCAAGCAAGGCTGGCTCCACAAAAAAGGGGGGGGCTCCTCCACGCTGTCCAGGAGAAATTGGAAGAAGCG CTGGTTTGTCCTCCGCCAGTCCAAGCTGATGTACTTTGAAAACGACAGCGAGGAGAAGCTCAAGGGCACC GTAGAAGTGCGAACGGCAAAAGAGATCATAGATAACACCACCAAGGAGAATGGGATCGACATCATTATGG CCGATAGGACTTTCCACCTGATTGCAGAGTCCCCAGAAGATGCCAGCCAGTGGTTCAGCGTGCTGAGTCA GGTCCACGCGTCCACGGACCAGGAGATCCAGGAGATGCATGATGAGCAGGCAAACCCACAGAATGCTGTG GGCACCTTGGATGTGGGGCTGATTGATTCTGTGTGTGCCTCTGACAGCCCTGATAGACCCAACTCGTTTG TGATCATCACGGCCAACCGGGTGCTGCACTGCAACGCCGACACGCCGGAGGAGATGCACCACTGGATAAC CCTGCTGCAGAGGTCCAAAGGGGACACCAGAGTGGAGGGCCAGGAATTCATCGTGAGAGGATGGTTGCAC AAAGAGGTGAAGAACAGTCCGAAGATGTCTTCACTGAAACTGAAGAAACGGTGGTTTGTACTCACCCACA ATTCCCTGGATTACTACAAGAGTTCAGAGAAGAACGCGCTCAAACTGGGGACCCTGGTCCTCAACAGCCT CTGCTCTGTCGTCCCCCCAGATGAGAAGATATTCAAAGAGACAGGCTACTGGAACGTCACCGTGTACGGG CGCAAGCACTGTTACCGGCTCTACACCAAGCTGCTCAACGAGGCCACCCGGTGGTCCAGTGCCATTCAAA ACGTGACTGACACCAAGGCCCCGATCGACACCCCCACCCAGCAGCTGATTCAAGATATCAAGGAGAACTG CCTGAACTCGGATGTGGTGGAACAGATTTACAAGCGGAACCCGATCCTTCGATACACCCATCACCCCTTG CACTCCCCGCTCCTGCCCCTTCCGTATGGGGACATAAATCTCAACTTGCTCAAAGACAAAGGCTATACCA CCCTTCAGGATGAGGCCATCAAGATATTCAATTCCCTGCAGCAACTGGAGTCCATGTCTGACCCAATTCC AATAATCCAGGGCATCCTACAGACAGGGCATGACCTGCGACCTCTGCGGGACGAGCTGTACTGCCAGCTT ATCAAACAGACCAACAAAGTGCCCCACCCCGGCAGTGTGGGCAACCTGTACAGCTGGCAGATCCTGACAT GCCTGAGCTGCACCTTCCTGCCGAGTCGAGGGATTCTCAAGTATCTCAAGTTCCATCTGAAAAGGATACG GGAACAGTTTCCAGGAACCGAGATGGAAAAATACGCTCTCTTCACTTACGAATCTCTTAAGAAAACCAAA TGCCGAGAGTTTGTGCCTTCCCGAGATGAAATAGAAGCTCTGATCCACAGGCAGGAAATGACATCCACGG TCTATTGCCATGGCGGCGGCTCCTGCAAGATCACCATCAACTCCCACACCACTGCTGGGGAGGTGGTGGA GAAGCTGATCCGAGGCCTGGCCATGGAGGACAGCAGGAACATGTTTGCTTTGTTTGAATACAACGGCCAC GTCGACAAAGCCATTGAAAGTCGAACCGTCGTAGCTGATGTCTTAGCCAAGTTTGAAAAGCTGGCTGCCA CATCCGAGGTTGGGGACCTGCCATGGAAATTCTACTTCAAACTTTACTGCTTCCTGGACACAGACAACGT GCCAAAAGACAGTGTGGAGTTTGCATTTATGTTTGAACAGGCCCACGAAGCGGTTATCCATGGCCACCAT CCAGCCCCGGAAGAAAACCTCCAGGTTCTTGCTGCCCTGCGACTCCAGTATCTGCAGGGGGATTATACTC TGCACGCTGCCATCCCACCTCTCGAAGAGGTTTATTCCCTGCAGAGACTCAAGGCCCGCATCAGCCAGTC AACCAAAACCTTCACCCCTTGTGAACGGCTGGAGAAGAGGCGGACGAGCTTCCTAGAGGGGACCCTGAGG CGGAGCTTCCGGACAGGATCCGTGGTCCGGCAGAAGGTCGAGGAGGAGCAGATGCTGGACATGTGGATTA AGGAAGAAGTCTCCTCTGCTCGAGCCAGTATCATTGACAAGTGGAGGAAATTTCAGGGAATGAACCAGGA ACAGGCCATGGCCAAGTACATGGCCTTGATCAAGGAGTGGCCTGGCTATGGCTCGACGCTGTTTGATGTG GAGTGCAAGGAAGGTGGCTTCCCTCAGGAACTCTGGTTGGGTGTCAGCGCGGACGCCGTCTCCGTCTACA AGCGTGGAGAGGGAAGACCACTGGAAGTCTTCCAGTATGAACACATCCTCTCTTTTGGGGCACCCCTGGC GAATACGTATAAGATCGTGGTCGATGAGAGGGAGCTGCTCTTTGAAACCAGTGAGGTGGTGGATGTGGCC AAGCTCATGAAAGCCTACATCAGCATGATCGTGAAGAAGCGCTACAGCACGACACGCTCCGCCAGCAGCC AGGGCAGCTCCAGG ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216606 representing NM_012334
Red=Cloning site Green=Tags(s) MDNFFTEGTRVWLRENGQHFPSTVNSCAEGIVVFRTDYGQVFTYKQSTITHQKVTAMHPTNEEGVDDMAS LTELHGGSIMYNLFQRYKRNQIYTYIGSILASVNPYQPIAGLYEPATMEQYSRRHLGELPPHIFAIANEC YRCLWKRYDNQCILISGESGAGKTESTKLILKFLSVISQQSLELSLKEKTSCVERAILESSPIMEAFGNA KTVYNNNSSRFGKFVQLNICQKGNIQGGRIVDYLLEKNRVVRQNPGERNYHIFYALLAGLEHEEREEFYL STPENYHYLNQSGCVEDKTISDQESFREVITAMDVMQFSKEEVREVSRLLAGILHLGNIEFITAGGAQVS FKTALGRSAELLGLDPTQLTDALTQRSMFLRGEEILTPLNVQQAVDSRDSLAMALYACCFEWVIKKINSR IKGNEDFKSIGILDIFGFENFEVNHFEQFNINYANEKLQEYFNKHIFSLEQLEYSREGLVWEDIDWIDNG ECLDLIEKKLGLLALINEESHFPQATDSTLLEKLHSQHANNHFYVKPRVAVNNFGVKHYAGEVQYDVRGI LEKNRDTFRDDLLNLLRESRFDFIYDLFEHVSSRNNQDTLKCGSKHRRPTVSSQFKDSLHSLMATLSSSN PFFVRCIKPNMQKMPDQFDQAVVLNQLRYSGMLETVRIRKAGYAVRRPFQDFYKRYKVLMRNLALPEDVR GKCTSLLQLYDASNSEWQLGKTKVFLRESLEQKLEKRREEEVSHAAMVIRAHVLGFLARKQYRKVLYCVV IIQKNYRAFLLRRRFLHLKKAAIVFQKQLRGQIARRVYRQLLAEKREQEEKKKQEEEEKKKREEEERERE RERREAELRAQQEEETRKQQELEALQKSQKEAELTRELEKQKENKQVEEILRLEKEIEDLQRMKEQQELS LTEASLQKLQERRDQELRRLEEEACRAAQEFLESLNFDEIDECVRNIERSLSVGSEFSSELAESACEEKP NFNFSQPYPEEEVDEGFEADDDAFKDSPNPSEHGHSDQRTSGIRTSDDSSEEDPYMNDTVVPTSPSADST VLLAPSVQDSGSLHNSSSGESTYCMPQNAGDLPSPDGDYDYDQDDYEDGAITSGSSVTFSNSYGSQWSPD YRCSVGTYNSSGAYRFSSEGAQSSFEDSEEDFDSRFDTDDELSYRRDSVYSCVTLPYFHSFLYMKGGLMN SWKRRWCVLKDETFLWFRSKQEALKQGWLHKKGGGSSTLSRRNWKKRWFVLRQSKLMYFENDSEEKLKGT VEVRTAKEIIDNTTKENGIDIIMADRTFHLIAESPEDASQWFSVLSQVHASTDQEIQEMHDEQANPQNAV GTLDVGLIDSVCASDSPDRPNSFVIITANRVLHCNADTPEEMHHWITLLQRSKGDTRVEGQEFIVRGWLH KEVKNSPKMSSLKLKKRWFVLTHNSLDYYKSSEKNALKLGTLVLNSLCSVVPPDEKIFKETGYWNVTVYG RKHCYRLYTKLLNEATRWSSAIQNVTDTKAPIDTPTQQLIQDIKENCLNSDVVEQIYKRNPILRYTHHPL HSPLLPLPYGDINLNLLKDKGYTTLQDEAIKIFNSLQQLESMSDPIPIIQGILQTGHDLRPLRDELYCQL IKQTNKVPHPGSVGNLYSWQILTCLSCTFLPSRGILKYLKFHLKRIREQFPGTEMEKYALFTYESLKKTK CREFVPSRDEIEALIHRQEMTSTVYCHGGGSCKITINSHTTAGEVVEKLIRGLAMEDSRNMFALFEYNGH VDKAIESRTVVADVLAKFEKLAATSEVGDLPWKFYFKLYCFLDTDNVPKDSVEFAFMFEQAHEAVIHGHH PAPEENLQVLAALRLQYLQGDYTLHAAIPPLEEVYSLQRLKARISQSTKTFTPCERLEKRRTSFLEGTLR RSFRTGSVVRQKVEEEQMLDMWIKEEVSSARASIIDKWRKFQGMNQEQAMAKYMALIKEWPGYGSTLFDV ECKEGGFPQELWLGVSADAVSVYKRGEGRPLEVFQYEHILSFGAPLANTYKIVVDERELLFETSEVVDVA KLMKAYISMIVKKRYSTTRSASSQGSSR TRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012334 |
| ORF Size | 6174 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012334.1, NP_036466.1 |
| RefSeq Size | 7787 bp |
| RefSeq ORF | 6177 bp |
| Locus ID | 4651 |
| UniProt ID | Q9HD67 |
| Domains | IQ, B41, MyTH4, myosin_head, PH |
| Protein Pathways | Fc gamma R-mediated phagocytosis |
| Gene Summary | This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-10 (MYH10). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. This gene functions as an actin-based molecular motor and plays a role in integration of F-actin and microtubule cytoskeletons during meiosis. [provided by RefSeq, Dec 2011] |
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