United States
Germany
Japan
United Kingdom
China
ROBO3 (NM_022370) Human Tagged ORF Clone
CAT＃: RG216411
- TrueORF®
ROBO3 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3)
ORF Plasmid: DDK
"NM_022370" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 15,300.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HGPPS; HGPPS1; HGPS; RBIG1; RIG1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216411 representing NM_022370
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCTACCTGCTGAAAACGCTGCTGCAGATGAACTTGTTCGCGGACTCTCTGGCCGGGGACATCT CCAACTCCAGCGAGCTGCTCTTGGGCTTCAACTCCTCGCTGGCGGCGCTCAACCACACCCTGCTGCCTCC CGGCGATCCCTCTCTCAACGGGTCAAGGGTAGGACCGGAGGACGCTATGCCCCGCATCGTGGAGCAGCCG CCAGATCTGCTGGTCTCCCGAGGCGAGCCCGCCACGTTGCCCTGCCGCGCTGAAGGCCGACCCCGACCCA ACATTGAGTGGTACAAGAACGGGGCGCGTGTGGCCACTGTGCGGGAGGATCCGCGTGCGCACCGCCTGCT GCTGCCCAGCGGCGCCCTCTTCTTCCCGCGCATCGTGCACGGGCGCCGCGCGCGGCCGGACGAAGGTGTC TACACTTGCGTGGCTCGCAACTACCTGGGGGCAGCAGCGAGCAGAAACGCCTCGCTGGAAGTGGCAGTCC TCCGTGATGATTTCCGGCAGTCTCCTGGAAACGTGGTGGTGGCAGTGGGGGAGCCAGCAGTACTGGAATG CGTGCCCCCCCGCGGCCACCCGGAGCCTTCCGTGTCCTGGAGGAAGGACGGTGCAAGACTCAAGGAAGAG GAAGGAAGGATCACGATCCGTGGAGGGAAGCTGATGATGTCACATACACTCAAGAGCGATGCAGGCATGT ATGTGTGCGTAGCCTCCAACATGGCGGGAGAACGGGAGAGTGCGGCAGCTGAAGTCATGGTACTGGAGCG TCCCTCATTCCTGCGCAGACCAGTGAATCAGGTGGTCCTGGCTGATGCCCCTGTGACTTTCCTATGTGAG GTGAAGGGGGATCCCCCACCTCGTCTACGCTGGCGCAAGGAGGATGGGGAACTGCCCACAGGCAGGTATG AGATCCGGAGTGACCACAGCCTTTGGATTGGGCATGTGAGTGCCGAAGATGAGGGAACGTACACCTGTGT GGCGGAGAACAGTGTGGGCCGCGCTGAAGCATCTGGCTCCCTCAGTGTTCACGTCCCACCCCAGTTGGTG ACCCAGCCCCAGGACCAGATGGCAGCTCCTGGAGAGAGCGTGGCTTTCCAGTGCGAGACCAAAGGAAACC CCCCACCTGCCATCTTCTGGCAGAAGGAGGGGAGTCAGGTCCTGCTTTTCCCCAGTCAGTCACTTCAGCC GACGGGGCGCTTCTCAGTGTCTCCAAGAGGCCAACTTAACATCACCGCGGTGCAGCGTGGGGATGCTGGG TACTACGTGTGCCAGGCTGTCAGTGTGGCTGGCAGCATCCTGGCCAAGGCCCTGCTGGAGATAAAAGGAG CCTCTTTGGATGGGCTGCCTCCTGTCATCCTCCAGGGACCAGCCAATCAGACGCTGGTGCTTGGCTCCTC CGTGTGGCTGCCCTGCAGAGTGACTGGGAACCCTCAACCCAGTGTCCGATGGAAGAAGGATGGGCAGTGG CTGCAGGGGGATGACCTCCAGTTCAAGACAATGGCCAACGGTACCCTGTACATCGCCAATGTGCAGGAGA TGGACATGGGCTTCTACAGCTGCGTGGCCAAGAGTTCCACAGGGGAAGCCACATGGAGCGGCTGGCTTAA GATGCGGGAAGACTGGGGAGTATCACCAGACCCCCCTACAGAACCCAGTTCCCCTCCGGGGGCTCCCTCT CAGCCAGTGGTCACTGAGATCACCAAGAACAGCATTACCCTGACCTGGAAGCCCAACCCACAAACTGGGG CTGCAGTCACGTCTTATGTGATAGAGGCCTTCAGCCCAGCAGCTGGCAACACATGGCGTACTGTGGCAGA TGGCGTGCAGCTGGAGACACACACAGTCAGCGGTCTGCAGCCCAATACCATCTACCTGTTTCTGGTTCGA GCAGTGGGAGCCTGGGGCCTCAGTGAGCCCAGCCCCGTCTCTGAGCCTGTCCGTACACAGGATAGCAGCC CCTCTAGGCCAGTGGAGGACCCATGGAGAGGCCAGCAGGGACTGGCGGAAGTGGCTGTGCGCCTGCAGGA GCCCATAGTCCTGGGACCCCGGACCCTGCAGGTGTCCTGGACTGTGGATGGCCCAGTCCAGCTGGTGCAA GGTTTCCGGGTGTCTTGGAGGGTAGCAGGCCCTGAGGGAGGAAGCTGGACAATGTTGGACCTACAGTCCC CAAGCCAGCAAAGTACTGTGCTAAGAGGACTCCCTCCAGGGACCCAAATCCAGATCAAGGTGCAAGCCCA AGGCCAGGAGGGGCTGGGGGCTGAAAGCCTCTCTGTGACCAGGAGCATTCCTGAGGAGGCCCCCAGTGGC CCCCCACAGGGAGTGGCGGTGGCCTTGGGGGGTGATGGCAACAGCAGTATCACTGTGTCCTGGGAACCTC CACTCCCCTCCCAGCAAAATGGGGTCATCACGGAATACCAGATCTGGTGCCTGGGCAATGAGAGCCGCTT TCACCTCAATCGATCTGCAGCAGGCTGGGCACGCTCCGCAATGCTCCGAGGACTGGTGCCCGGTCTCCTC TATCGAACCCTGGTCGCGGCGGCCACCAGCGCAGGCGTGGGCGTGCCCAGTGCCCCAGTGCTGGTGCAGC TGCCGTCCCCGCCGGACCTGGAGCCCGGGCTGGAGGTGGGCGCGGGGCTGGCGGTGCGGCTGGCGAGGGT GCTGCGGGAGCCCGCCTTCCTCGCGGGCAGCGGCGCAGCCTGCGGGGCGCTGCTTCTCGGGCTCTGCGCC GCCCTCTACTGGCGCCGGAAACAGCGCAAAGAGCTCAGCCACTACACGGCCTCTTTTGCCTACACACCGG CAGTGTCCTTCCCGCACTCAGAGGGCCTCTCTGGAGCCAGTTCCAGGCCACCCATGGGCCTTGGCCCCGC CCCCTACTCATGGCTGGCAGATTCGTGGCCCCACCCATCTCGAAGCCCCTCGGCCCAGGAACCCAGGGGA AGCTGCTGCCCTAGCAATCCTGACCCGGACGACAGATATTACAACGAAGCGGGAATCTCCCTGTATCTAG CTCAGACGGCCAGGGGCACGGCCGCCCCTGGCGAGGGTCCTGTCTATAGCACCATTGACCCAGCGGGGGA GGAGCTGCAGACCTTCCATGGGGGCTTCCCCCAACATCCCTCAGGAGATCTGGGTCCCTGGAGCCAGTAC GCTCCTCCAGAGTGGAGCCAGGGGGACAGTGGAGCCAAGGGAGGCAAAGTGAAGCTTCTGGGGAAACCTG TGCAGATGCCCTCTCTGAACTGGCCAGAAGCCCTGCCCCCACCTCCTCCTTCTTGTGAACTGAGCTGCCT AGAAGGGCCGGAGGAGGAGCTGGAGGGCAGCTCAGAGCCAGAGGAGTGGTGCCCGCCAATGCCTGAGAGA AGTCACCTGACGGAGCCCAGCTCCAGTGGAGGGTGCCTGGTCACCCCATCCCGAAGGGAAACCCCCTCTC CCACACCTTCCTATGGACAGCAGTCCACAGCCACTCTTACACCCTCACCTCCTGACCCTCCCCAGCCCCC AACTGACATGCCCCATCTCCATCAGATGCCCAGGAGGGTGCCCCTTGGGCCGAGTTCCCCTCTCAGTGTA TCCCAGCCCATGCTGGGCATCCGTGAAGCGAGGCCTGCTGGCTTGGGTGCTGGCCCTGCAGCCTCACCCC ACCTCAGCCCCAGTCCTGCCCCTAGCACAGCCAGCAGTGCCCCAGGCAGAACCTGGCAGGGGAATGGGGA GATGACTCCCCCACTTCAAGGACCCCGTGCTCGATTCCGGAAGAAACCCAAGGCTCTTCCCTACAGGAGG GAGAACAGTCCTGGGGACTTGCCCCCACCACCCTTGCCACCGCCAGAGGAAGAGGCGAGCTGGGCCCTAG AGCTGAGGGCAGCAGGCAGCATGTCCTCCCTGGAGCGGGAGCGCAGTGGGGAGAGGAAAGCGGTCCAGGC CGTGCCCCTGGCAGCCCAGCGGGTGCTCCACCCAGATGAAGAGGCCTGGCTCCCATACAGCAGACCAAGC TTCCTGTCCCGGGGCCAGGGCACCAGCACATGTTCCACGGCCGGCAGCAACTCTTCCAGGGGCTCCAGCA GCTCTAGGGGCTCCCGGGGCCCTGGCCGGAGCCGGAGTCGGAGTCAGAGCCGGAGCCAGAGCCAAAGGCC AGGACAGAAACGCCGAGAGGAACCAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216411 representing NM_022370
Red=Cloning site Green=Tags(s) MLRYLLKTLLQMNLFADSLAGDISNSSELLLGFNSSLAALNHTLLPPGDPSLNGSRVGPEDAMPRIVEQP PDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRIVHGRRARPDEGV YTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARLKEE EGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCE VKGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVPPQLV TQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAG YYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQW LQGDDLQFKTMANGTLYIANVQEMDMGFYSCVAKSSTGEATWSGWLKMREDWGVSPDPPTEPSSPPGAPS QPVVTEITKNSITLTWKPNPQTGAAVTSYVIEAFSPAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVR AVGAWGLSEPSPVSEPVRTQDSSPSRPVEDPWRGQQGLAEVAVRLQEPIVLGPRTLQVSWTVDGPVQLVQ GFRVSWRVAGPEGGSWTMLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGAESLSVTRSIPEEAPSG PPQGVAVALGGDGNSSITVSWEPPLPSQQNGVITEYQIWCLGNESRFHLNRSAAGWARSAMLRGLVPGLL YRTLVAAATSAGVGVPSAPVLVQLPSPPDLEPGLEVGAGLAVRLARVLREPAFLAGSGAACGALLLGLCA ALYWRRKQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYSWLADSWPHPSRSPSAQEPRG SCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPGEGPVYSTIDPAGEELQTFHGGFPQHPSGDLGPWSQY APPEWSQGDSGAKGGKVKLLGKPVQMPSLNWPEALPPPPPSCELSCLEGPEEELEGSSEPEEWCPPMPER SHLTEPSSSGGCLVTPSRRETPSPTPSYGQQSTATLTPSPPDPPQPPTDMPHLHQMPRRVPLGPSSPLSV SQPMLGIREARPAGLGAGPAASPHLSPSPAPSTASSAPGRTWQGNGEMTPPLQGPRARFRKKPKALPYRR ENSPGDLPPPPLPPPEEEASWALELRAAGSMSSLERERSGERKAVQAVPLAAQRVLHPDEEAWLPYSRPS FLSRGQGTSTCSTAGSNSSRGSSSSRGSRGPGRSRSRSQSRSQSQRPGQKRREEPR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022370 |
| ORF Size | 4158 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022370.4 |
| RefSeq Size | 4554 bp |
| RefSeq ORF | 4161 bp |
| Locus ID | 64221 |
| UniProt ID | Q96MS0 |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance |
| Gene Summary | This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. [provided by RefSeq, May 2019] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216411 | ROBO3 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 14,224.00 |
|
| RC216411L1 | Lenti-ORF clone of ROBO3 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 16,624.00 |
|
| RC216411L2 | Lenti-ORF clone of ROBO3 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,770.00 |
|
| RC216411L3 | Lenti-ORF clone of ROBO3 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,770.00 |
|
| RC216411L4 | Lenti-ORF clone of ROBO3 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,770.00 |
|
| SC308726 | ROBO3 (untagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,576.00 |
