Phospholipase C gamma 1 (PLCG1) (NM_182811) Human Tagged ORF Clone
CAT#: RG216391
- TrueORF®
PLCG1 (tGFP-tagged) - Human phospholipase C, gamma 1 (PLCG1), transcript variant 2
ORF Plasmid: DDK
"NM_182811" in other vectors (6)
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CNY 14,160.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216391 representing NM_182811
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGCCGCGTCCCCTTGCGCCAACGGCTGCGGGCCCGGCGCGCCCTCGGACGCCGAGGTGCTGC ACCTCTGCCGCAGCCTCGAGGTGGGCACCGTCATGACTTTGTTCTACTCCAAGAAGTCGCAGCGACCCGA GCGGAAGACCTTCCAGGTCAAGCTGGAGACGCGCCAGATCACGTGGAGCCGGGGCGCCGACAAGATCGAG GGGGCCATTGACATTCGTGAAATTAAGGAGATCCGCCCAGGGAAGACCTCACGGGACTTTGATCGCTATC AAGAGGACCCAGCTTTCCGGCCGGACCAGTCACATTGCTTTGTCATTCTCTATGGAATGGAATTTCGCCT GAAAACGCTGAGCCTGCAAGCCACATCTGAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTG ATGGAGGATACATTGCAGGCACCCACACCCCTGCAGATTGAGAGGTGGCTCCGGAAGCAGTTTTACTCAG TGGATCGGAATCGTGAGGATCGTATATCAGCCAAGGACCTGAAGAACATGCTGTCCCAGGTCAACTACCG GGTCCCCAACATGCGCTTCCTCCGAGAGCGGCTGACGGACCTGGAGCAGCGCAGCGGGGACATCACCTAC GGGCAGTTTGCTCAGCTGTACCGCAGCCTCATGTACAGCGCCCAGAAGACGATGGACCTCCCCTTCTTGG AAGCCAGTACTCTGAGGGCTGGGGAGCGGCCGGAGCTTTGCCGAGTGTCCCTTCCTGAGTTCCAGCAGTT CCTTCTTGACTACCAGGGGGAGCTGTGGGCTGTTGATCGCCTCCAGGTGCAGGAGTTCATGCTCAGCTTC CTCCGAGACCCCTTACGAGAGATCGAGGAGCCATACTTCTTCCTGGATGAGTTTGTCACCTTCCTGTTCT CCAAAGAGAACAGTGTGTGGAACTCGCAGCTGGATGCAGTATGCCCGGACACCATGAACAACCCTCTTTC CCACTACTGGATCTCCTCCTCGCACAACACGTACCTGACCGGGGACCAGTTCTCCAGTGAGTCCTCCTTG GAAGCCTATGCTCGCTGCCTGCGGATGGGCTGTCGCTGCATTGAGTTGGACTGCTGGGACGGCCCGGATG GGATGCCAGTTATTTACCATGGGCACACCCTTACCACCAAGATCAAGTTCTCAGATGTCCTGCACACCAT CAAGGAGCATGCCTTTGTGGCCTCAGAGTACCCAGTCATCCTGTCCATTGAGGACCACTGCAGCATTGCC CAGCAGAGAAACATGGCCCAATACTTCAAGAAGGTGCTGGGGGACACACTCCTCACCAAGCCCGTGGAGA TCTCTGCCGACGGGCTCCCCTCACCCAACCAGCTTAAGAGGAAGATCCTCATCAAGCACAAGAAGCTGGC TGAGGGCAGTGCCTACGAGGAGGTGCCTACATCCATGATGTACTCTGAGAACGACATCAGCAACTCTATC AAGAATGGCATCCTCTACCTGGAGGACCCTGTGAACCACGAATGGTATCCCCACTACTTTGTTCTGACCA GCAGCAAGATCTACTACTCTGAGGAGACCAGCAGTGACCAGGGCAACGAGGATGAGGAGGAGCCCAAGGA GGTCAGCAGCAGCACAGAGCTGCACTCCAATGAGAAGTGGTTCCATGGGAAGCTAGGGGCAGGGCGTGAC GGGCGTCACATCGCTGAGCGCCTGCTTACTGAGTACTGCATCGAGACCGGAGCCCCTGACGGCTCCTTCC TCGTGCGAGAGAGTGAGACCTTCGTGGGCGACTACACGCTCTCTTTCTGGCGGAACGGGAAAGTCCAGCA CTGCCGTATCCACTCCCGGCAAGATGCTGGGACCCCCAAGTTCTTCTTGACAGACAACCTCGTCTTTGAC TCCCTCTATGACCTCATCACGCACTACCAGCAGGTGCCCCTGCGCTGTAATGAGTTTGAGATGCGACTTT CAGAGCCTGTCCCACAGACCAACGCCCACGAGAGCAAAGAGTGGTACCACGCGAGCCTGACCAGAGCACA GGCTGAGCACATGCTAATGCGCGTCCCTCGTGATGGGGCCTTCCTGGTGCGGAAGCGGAATGAACCCAAC TCATATGCCATCTCTTTCCGGGCTGAGGGCAAGATCAAGCATTGCCGTGTCCAGCAAGAGGGCCAGACAG TGATGCTAGGGAACTCGGAGTTCGACAGCCTTGTTGACCTCATCAGCTACTATGAGAAACACCCGCTATA CCGCAAGATGAAGCTGCGCTATCCCATCAACGAGGAGGCACTGGAGAAGATTGGCACAGCTGAGCCTGAC TACGGGGCCCTGTATGAGGGACGCAACCCTGGCTTCTATGTAGAGGCAAACCCTATGCCAACTTTCAAGT GTGCAGTCAAAGCCCTCTTTGACTACAAGGCCCAGAGGGAGGACGAGCTGACCTTCATCAAGAGCGCCAT CATCCAGAATGTGGAGAAGCAAGAGGGAGGCTGGTGGCGAGGGGACTACGGAGGGAAGAAGCAGCTGTGG TTCCCATCAAACTACGTGGAAGAGATGGTCAACCCCGTGGCCCTGGAGCCGGAGAGGGAGCACTTGGACG AGAACAGCCCCCTAGGGGACTTGCTGCGGGGGGTCTTGGATGTGCCGGCTTGTCAGATTGCCATCCGTCC TGAGGGCAAGAACAACCGGCTCTTCGTCTTCTCCATCAGCATGGCGTCGGTGGCCCACTGGTCCCTGGAT GTTGCTGCCGACTCACAGGAGGAGCTGCAGGACTGGGTGAAAAAGATCCGTGAAGTGGCCCAGACAGCAG ACGCCAGGCTCACTGAAGGGAAGATAATGGAACGGAGGAAGAAGATTGCCCTGGAGCTCTCTGAACTTGT CGTCTACTGCCGGCCTGTTCCCTTTGATGAAGAGAAGATTGGCACAGAACGTGCTTGCTACCGGGACATG TCATCCTTCCCGGAAACCAAGGCTGAGAAATACGTGAACAAGGCCAAAGGCAAGAAGTTCCTTCAGTACA ATCGACTGCAGCTCTCCCGCATCTACCCCAAGGGCCAGCGACTGGATTCCTCCAACTACGATCCTTTGCC CATGTGGATCTGTGGCAGTCAGCTTGTGGCCCTCAACTTCCAGACCCCTGACAAGCCTATGCAGATGAAC CAGGCCCTCTTCATGACGGGCAGGCACTGTGGCTACGTGCTGCAGCCAAGCACCATGCGGGATGAGGCCT TCGACCCCTTTGACAAGAGCAGCCTCCGCGGGCTGGAGCCATGTGCCATCTCTATTGAGGTGCTGGGGGC CCGACATCTGCCAAAGAATGGCCGAGGCATTGTGTGTCCTTTTGTGGAGATTGAGGTGGCTGGAGCTGAG TATGACAGCACCAAGCAGAAGACAGAGTTTGTGGTGGACAATGGACTCAACCCTGTATGGCCAGCCAAGC CCTTCCACTTCCAGATCAGTAACCCTGAATTTGCCTTTCTGCGCTTCGTGGTGTATGAGGAAGACATGTT TAGTGACCAGAATTTCCTGGCTCAGGCTACTTTCCCAGTAAAAGGCCTGAAGACAGGATACAGAGCAGTG CCTTTGAAGAACAACTACAGTGAGGACCTGGAGTTGGCCTCCCTGCTGATCAAGATTGACATTTTCCCTG CCAAGGAGAATGGTGACCTCAGTCCCTTCAGTGGTACGTCCCTGCGGGAGCGGGGCTCAGATGCCTCAGG CCAGCTGTTTCATGGCCGAGCCCGGGAAGGCTCCTTTGAATCCCGCTACCAGCAGCCGTTTGAGGACTTC CGCATCTCCCAGGAGCATCTCGCAGACCATTTTGACAGTCGAGAACGAAGGGCCCCAAGAAGGACTCGGG TCAATGGAGACAACCGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216391 representing NM_182811
Red=Cloning site Green=Tags(s) MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIE GAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWL MEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITY GQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSF LRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSL EAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIA QQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSI KNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRD GRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFD SLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPN SYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPD YGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLW FPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLD VAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDM SSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMN QALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAE YDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAV PLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDF RISQEHLADHFDSRERRAPRRTRVNGDNRL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_182811 |
ORF Size | 3870 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182811.2 |
RefSeq Size | 5202 bp |
RefSeq ORF | 3873 bp |
Locus ID | 5335 |
UniProt ID | P19174 |
Protein Families | Druggable Genome |
Protein Pathways | Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection |
Gene Summary | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216391 | PLCG1 (Myc-DDK-tagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2 |
CNY 8,984.00 |
|
RC216391L1 | Lenti-ORF clone of PLCG1 (Myc-DDK-tagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2 |
CNY 11,384.00 |
|
RC216391L2 | Lenti-ORF clone of PLCG1 (mGFP-tagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2 |
CNY 14,730.00 |
|
RC216391L3 | Lenti-ORF clone of PLCG1 (Myc-DDK-tagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2 |
CNY 14,730.00 |
|
RC216391L4 | Lenti-ORF clone of PLCG1 (mGFP-tagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2 |
CNY 14,730.00 |
|
SC107651 | PLCG1 (untagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2 |
CNY 8,992.00 |
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