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MYH15 (NM_014981) Human Tagged ORF Clone
CAT＃: RG215967
- TrueORF®
MYH15 (tGFP-tagged) - Human myosin, heavy chain 15 (MYH15)
ORF Plasmid: DDK
"NM_014981" in other vectors (2)
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CNY 20,900.00
货期*
8周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215967 representing NM_014981
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGAATCTTGCCTACTCACGTTTAGGGCCTTTTTTTGGTGGATTGCTTTAATAAAGATGGATCTGT CAGACCTTGGAGAAGCCGCAGCCTTCCTCAGAAGAAGTGAAGCTGAGCTGCTTCTACTACAGGCCACAGC CTTGGATGGGAAGAAGAAATGCTGGATTCCTGATGGTGAGAATGCTTATATCGAGGCTGAGGTAAAAGGG AGTGAAGATGATGGAACAGTAATTGTTGAGACAGCAGATGGAGAGAGTCTGAGCATAAAGGAGGACAAAA TCCAGCAGATGAATCCTCCAGAGTTTGAAATGATTGAAGACATGGCAATGCTGACTCACCTCAATGAGGC ATCCGTGCTGCATACCCTGAAGCGGCGCTATGGCCAGTGGATGATCTATACATATTCAGGTCTCTTCTGT GTGACCATAAACCCTTACAAATGGCTTCCCGTGTATCAGAAAGAAGTCATGGCCGCCTACAAAGGGAAGA GGCGATCAGAGGCTCCCCCTCACATCTTTGCTGTTGCCAATAACGCCTTTCAGGACATGCTTCACAATCG AGAAAATCAGTCTATACTCTTCACAGGAGAATCTGGTGCTGGAAAGACTGTGAACAGCAAACATATTATC CAGTATTTTGCCACCATAGCAGCCATGATTGAATCCAGGAAAAAGCAGGGGGCGTTAGAAGATCAAATCA TGCAAGCGAATACTATCTTGGAAGCATTTGGAAATGCTAAAACCCTGAGAAATGACAACTCCTCTCGTTT TGGCAAATTCATCAGGATGCACTTTGGTGCCAGAGGCATGCTGTCATCTGTGGACATTGATATCTATTTG CTTGAAAAGTCCAGGGTGATTTTCCAGCAGGCTGGAGAGAGGAACTACCACATATTCTATCAAATTCTAT CTGGACAAAAAGAGCTTCATGACCTGCTCCTGGTATCTGCAAATCCCTCAGACTTCCACTTTTGCTCCTG TGGAGCAGTTACTGTGGAGAGCTTGGATGATGCTGAAGAATTGCTGGCCACAGAACAAGCCATGGACATC TTGGGCTTTCTTCCTGATGAGAAGTATGGATGCTATAAACTCACTGGAGCCATCATGCACTTTGGAAATA TGAAATTTAAACAGAAACCTAGAGAAGAGCAACTGGAAGCAGATGGCACAGAAAATGCTGACAAAGCTGC TTTCCTCATGGGCATTAACTCCTCTGAGTTGGTAAAGTGCTTGATCCATCCTAGAATCAAAGTTGGTAAC GAATATGTTACCAGAGGTCAAACTATAGAACAGGTAACCTGTGCTGTCGGTGCCCTGTCCAAGTCAATGT ATGAAAGGATGTTTAAGTGGCTAGTGGCACGGATCAACAGGGCCCTGGATGCCAAGCTGTCAAGGCAGTT CTTCATTGGCATTCTTGACATCACTGGTTTTGAAATCCTTGAGTATAATAGCCTTGAGCAACTTTGCATT AATTTTACCAATGAAAAATTACAACAATTCTTCAATTGGCACATGTTTGTTCTGGAGCAAGAGGAATATA AGAAAGAAAGCATTGAATGGGTGTCTATTGGCTTTGGTCTGGATTTGCAAGCTTGCATAGATCTCATTGA GAAGCCAATGGGCATCCTTTCCATCCTTGAAGAAGAGTGTATGTTTCCTAAGGCTACAGACCTGACTTTC AAGACCAAACTCTTTGACAACCATTTTGGAAAGTCGGTTCATCTCCAGAAGCCCAAGCCTGATAAGAAGA AATTTGAAGCTCATTTTGAACTTGTCCATTATGCAGGAGTGGTACCTTATAATATCAGTGGTTGGCTGGA AAAGAACAAAGACCTCCTTAATGAAACAGTGGTAGCTGTATTTCAGAAGTCTTCCAACAGACTCCTGGCG AGCCTTTTTGAAAATTACATGAGTACTGACAGTGCTATACCATTTGGGGAGAAGAAACGAAAGAAAGGAG CTTCATTCCAAACGGTTGCATCTCTGCATAAAGAAAACCTGAATAAATTGATGACTAATCTGAAATCAAC AGCACCTCATTTTGTGAGATGCATAAATCCCAATGTGAACAAAATACCAGGTATACTGGACCCTTACTTG GTTCTACAGCAGTTGCGCTGTAATGGTGTCTTGGAAGGGACTAGGATATGCCGTGAAGGTTTTCCAAACC GACTGCAGTATGCTGATTTTAAACAAAGGTACTGCATTCTGAATCCAAGGACCTTTCCAAAGAGCAAGTT TGTGAGCAGCAGAAAAGCAGCTGAAGAATTACTTGGCTCCTTGGAGATAGACCATACCCAGTACCGATTT GGAATCACTAAGGTGTTTTTTAAAGCTGGGTTTCTGGGCCAACTGGAAGCAATAAGAGATGAGAGACTAT CTAAAGTCTTCACATTGTTCCAAGCCAGAGCACAGGGCAAACTGATGCGAATCAAATTCCAGAAGATTCT GGAAGAAAGGGATGCACTTATTTTGATCCAATGGAACATAAGAGCTTTCATGGCTGTGAAGAACTGGCCC TGGATGAGGCTCTTCTTCAAGATCAAGCCTCTTGTTAAATCTTCAGAAGTAGGAGAAGAAGTAGCTGGAC TGAAGGAAGAGTGTGCACAATTACAGAAAGCCTTGGAGAAATCAGAGTTTCAGAGGGAGGAACTGAAAGC AAAGCAAGTATCCCTCACTCAGGAAAAAAATGACCTGATTCTTCAGCTTCAGGCTGAGCAAGAGACACTG GCAAATGTTGAAGAGCAGTGCGAGTGGCTGATTAAATCCAAGATCCAGCTGGAGGCCAGAGTAAAGGAGC TGTCGGAGAGGGTGGAGGAAGAAGAGGAGATAAATTCTGAGCTGACTGCCAGGGGGCGGAAACTCGAAGA TGAATGTTTTGAGTTGAAGAAAGAAATCGATGACCTGGAAACAATGTTGGTGAAGTCAGAGAAGGAGAAG CGTACTACAGAGCACAAGGTCAAGAACTTGACTGAGGAAGTAGAGTTTCTAAATGAGGATATCAGCAAAC TTAACAGAGCAGCCAAGGTTGTGCAGGAGGCCCATCAGCAGACCCTGGATGACCTGCACATGGAGGAGGA GAAGCTCAGCAGCCTGAGCAAAGCAAATCTGAAGCTGGAACAGCAAGTTGATGAGCTTGAGGGTGCCCTT GAGCAGGAGAGAAAAGCGAGAATGAACTGTGAAAGGGAACTGCACAAACTGGAGGGCAATTTAAAGCTGA ATCGGGAAAGTATGGAGAACCTGGAAAGCAGCCAGCGACACCTGGCAGAAGAGCTGAGGAAAAAAGAATT AGAATTGAGTCAGATGAATTCAAAAGTGGAGAATGAGAAAGGCCTGGTAGCTCAGCTTCAGAAGACGGTT AAAGAGCTTCAGACTCAAATAAAGGATTTGAAAGAGAAACTAGAAGCTGAAAGGACCACTCGAGCCAAGA TGGAAAGGGAGAGAGCTGACCTCACCCAAGACCTGGCTGACTTGAATGAGAGGCTGGAGGAGGTAGGAGG ATCCAGTTTGGCTCAGCTGGAAATAACTAAGAAACAGGAAACCAAATTCCAGAAGCTGCACCGAGACATG GAAGAGGCCACTCTGCACTTTGAGACAACTTCTGCATCTTTGAAGAAGAGACATGCAGACAGCCTGGCTG AGCTCGAGGGCCAGGTAGAAAATCTACAGCAGGTCAAGCAGAAACTGGAAAAAGACAAGAGTGACTTGCA GCTAGAAGTAGATGACCTCCTGACCCGTGTTGAGCAGATGACAAGAGCTAAGGCAAATGCTGAGAAACTC TGTACTCTATATGAAGAGCGCTTGCATGAAGCAACTGCAAAGCTAGATAAGGTGACTCAGTTGGCAAATG ACCTGGCAGCACAAAAGACAAAGCTGTGGAGTGAGAGTGGCGAGTTCCTACGGAGGCTTGAAGAGAAGGA GGCTCTGATAAACCAACTTTCCAGGGAAAAGAGCAACTTCACTCGGCAGATTGAAGACCTGAGAGGGCAG CTGGAAAAGGAGACCAAATCCCAGAGTGCCCTGGCCCATGCCCTGCAGAAGGCTCAGCGTGACTGTGACC TTCTACGAGAGCAGTATGAGGAAGAACAAGAGGTCAAGGCTGAGCTGCACCGGACCTTATCCAAAGTCAA TGCTGAAATGGTGCAATGGAGAATGAAGTATGAAAACAATGTCATCCAGAGAACAGAAGACTTGGAGGAT GCCAAGAAGGAACTGGCAATTAGATTGCAGGAGGCAGCCGAAGCCATGGGGGTGGCCAATGCCAGAAATG CCTCCTTGGAGAGAGCCAGGCACCAGCTGCAGCTGGAGCTCGGGGACGCCCTGTCTGACCTCGGGAAGGT CCGCTCTGCAGCAGCCAGGCTGGACCAGAAGCAGCTGCAGTCTGGCAAGGCCCTTGCCGACTGGAAGCAG AAGCACGAGGAGTCCCAGGCGTTGCTGGATGCCTCTCAGAAGGAAGTTCAGGCTCTCAGTACAGAGCTCC TCAAGCTCAAGAACACCTATGAGGAGAGCATCGTGGGCCAGGAGACACTCAGGAGGGAGAACAAGAACCT CCAAGAAGAGATTTCTAATCTGACAAACCAGGTTAGAGAAGGGACCAAGAACTTAACTGAAATGGAAAAG GTCAAGAAACTAATTGAAGAAGAGAAGACAGAAGTCCAGGTGACACTGGAAGAAACAGAGGGAGCCCTGG AACGTAATGAAAGCAAGATTCTTCATTTCCAGCTTGAACTCTTGGAAGCTAAAGCAGAACTTGAAAGAAA GCTTTCAGAGAAAGATGAAGAAATAGAAAATTTTAGGAGGAAGCAGCAGTGTACCATTGACTCCCTGCAG TCTAGTCTGGATTCTGAAGCTAAGAGCAGAATTGAGGTTACCCGGCTGAAGAAGAAGATGGAAGAGGACC TCAATGAGATGGAACTCCAGCTTAGCTGTGCCAACCGGCAGGTGTCAGAAGCAACCAAATCCCTGGGCCA GCTTCAGATTCAAATCAAGGACCTTCAAATGCAGCTGGATGACAGCACACAACTGAACAGTGATCTGAAG GAGCAGGTGGCTGTGGCTGAGCGGCGCAACTCTCTTCTTCAGTCTGAACTAGAGGATCTAAGGTCCCTGC AAGAGCAGACAGAGCGTGGCCGCAGGCTGTCAGAAGAAGAGCTCCTGGAAGCAACAGAAAGAATCAATCT TTTCTATACCCAGAACACAAGCCTCCTCAGCCAGAAGAAGAAACTGGAGGCTGATGTTGCCCGGATGCAG AAAGAAGCTGAAGAGGTGGTGCAGGAGTGTCAAAATGCAGAAGAGAAGGCCAAGAAGGCAGCCATTGAGG CAGCAAACTTGTCAGAAGAACTGAAGAAGAAGCAAGACACCATTGCCCACTTGGAAAGGACAAGAGAAAA TATGGAGCAGACAATTACAGACTTACAGAAAAGGCTGGCTGAAGCTGAACAGATGGCCCTGATGGGGAGT AGAAAGCAAATCCAGAAACTAGAATCCAGGGTTCGTGAACTGGAAGGTGAACTGGAGGGTGAAATCCGTC GCAGTGCAGAGGCCCAGAGGGGAGCCCGCAGACTTGAGCGATGCATCAAAGAGCTGACCTATCAGGCAGA GGAAGACAAGAAGAATCTGAGCAGGATGCAAACTCAGATGGATAAACTTCAGCTAAAAGTGCAAAATTAC AAGCAGCAAGTCGAGGTGGCGGAAACACAAGCCAATCAATACCTTTCCAAGTATAAGAAACAGCAACATG AGTTGAATGAAGTGAAGGAAAGGGCAGAGGTGGCAGAATCTCAAGTCAATAAACTCAAAATTAAAGCAAG AGAGTTTGGGAAAAAGGTTCAAGAAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215967 representing NM_014981
Red=Cloning site Green=Tags(s) MVESCLLTFRAFFWWIALIKMDLSDLGEAAAFLRRSEAELLLLQATALDGKKKCWIPDGENAYIEAEVKG SEDDGTVIVETADGESLSIKEDKIQQMNPPEFEMIEDMAMLTHLNEASVLHTLKRRYGQWMIYTYSGLFC VTINPYKWLPVYQKEVMAAYKGKRRSEAPPHIFAVANNAFQDMLHNRENQSILFTGESGAGKTVNSKHII QYFATIAAMIESRKKQGALEDQIMQANTILEAFGNAKTLRNDNSSRFGKFIRMHFGARGMLSSVDIDIYL LEKSRVIFQQAGERNYHIFYQILSGQKELHDLLLVSANPSDFHFCSCGAVTVESLDDAEELLATEQAMDI LGFLPDEKYGCYKLTGAIMHFGNMKFKQKPREEQLEADGTENADKAAFLMGINSSELVKCLIHPRIKVGN EYVTRGQTIEQVTCAVGALSKSMYERMFKWLVARINRALDAKLSRQFFIGILDITGFEILEYNSLEQLCI NFTNEKLQQFFNWHMFVLEQEEYKKESIEWVSIGFGLDLQACIDLIEKPMGILSILEEECMFPKATDLTF KTKLFDNHFGKSVHLQKPKPDKKKFEAHFELVHYAGVVPYNISGWLEKNKDLLNETVVAVFQKSSNRLLA SLFENYMSTDSAIPFGEKKRKKGASFQTVASLHKENLNKLMTNLKSTAPHFVRCINPNVNKIPGILDPYL VLQQLRCNGVLEGTRICREGFPNRLQYADFKQRYCILNPRTFPKSKFVSSRKAAEELLGSLEIDHTQYRF GITKVFFKAGFLGQLEAIRDERLSKVFTLFQARAQGKLMRIKFQKILEERDALILIQWNIRAFMAVKNWP WMRLFFKIKPLVKSSEVGEEVAGLKEECAQLQKALEKSEFQREELKAKQVSLTQEKNDLILQLQAEQETL ANVEEQCEWLIKSKIQLEARVKELSERVEEEEEINSELTARGRKLEDECFELKKEIDDLETMLVKSEKEK RTTEHKVKNLTEEVEFLNEDISKLNRAAKVVQEAHQQTLDDLHMEEEKLSSLSKANLKLEQQVDELEGAL EQERKARMNCERELHKLEGNLKLNRESMENLESSQRHLAEELRKKELELSQMNSKVENEKGLVAQLQKTV KELQTQIKDLKEKLEAERTTRAKMERERADLTQDLADLNERLEEVGGSSLAQLEITKKQETKFQKLHRDM EEATLHFETTSASLKKRHADSLAELEGQVENLQQVKQKLEKDKSDLQLEVDDLLTRVEQMTRAKANAEKL CTLYEERLHEATAKLDKVTQLANDLAAQKTKLWSESGEFLRRLEEKEALINQLSREKSNFTRQIEDLRGQ LEKETKSQSALAHALQKAQRDCDLLREQYEEEQEVKAELHRTLSKVNAEMVQWRMKYENNVIQRTEDLED AKKELAIRLQEAAEAMGVANARNASLERARHQLQLELGDALSDLGKVRSAAARLDQKQLQSGKALADWKQ KHEESQALLDASQKEVQALSTELLKLKNTYEESIVGQETLRRENKNLQEEISNLTNQVREGTKNLTEMEK VKKLIEEEKTEVQVTLEETEGALERNESKILHFQLELLEAKAELERKLSEKDEEIENFRRKQQCTIDSLQ SSLDSEAKSRIEVTRLKKKMEEDLNEMELQLSCANRQVSEATKSLGQLQIQIKDLQMQLDDSTQLNSDLK EQVAVAERRNSLLQSELEDLRSLQEQTERGRRLSEEELLEATERINLFYTQNTSLLSQKKKLEADVARMQ KEAEEVVQECQNAEEKAKKAAIEAANLSEELKKKQDTIAHLERTRENMEQTITDLQKRLAEAEQMALMGS RKQIQKLESRVRELEGELEGEIRRSAEAQRGARRLERCIKELTYQAEEDKKNLSRMQTQMDKLQLKVQNY KQQVEVAETQANQYLSKYKKQQHELNEVKERAEVAESQVNKLKIKAREFGKKVQEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014981 |
| ORF Size | 5838 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014981.2 |
| RefSeq Size | 7074 bp |
| RefSeq ORF | 5841 bp |
| Locus ID | 22989 |
| UniProt ID | Q9Y2K3 |
| Protein Pathways | Tight junction, Viral myocarditis |
| Gene Summary | Muscle contraction.[UniProtKB/Swiss-Prot Function] |
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