United States
Germany
Japan
United Kingdom
China
alpha Adducin (ADD1) (NM_014189) Human Tagged ORF Clone
CAT＃: RG215898
- TrueORF®
ADD1 (tGFP-tagged) - Human adducin 1 (alpha) (ADD1), transcript variant 2
ORF Plasmid: DDK
"NM_014189" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,784.00
CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADDA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215898 representing NM_014189
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGTGATTCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCCCCTCACAAGGAGAGGT ACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGGAGAGGAACATGGCACCAGACCTTCGCCA GGACTTCAACATGATGGAGCAAAAGAAGAGGGTGTCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAA TTGGAATCAATGATACAGGAGCAATTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGA TTGCAGATTTTATGACCACGAATGTACCAAATGTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTT AAACATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGTATGACAAA GGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTCTTTGGGTGGTCTCAGC TTATCTACAATCATATCACAACCAGAGTGAACTCCGAGCAGGAACACTTCCTCATTGTCCCTTTTGGGCT TCTTTACAGTGAAGTGACTGCATCCAGTTTGGTTAAGATCAATCTACAAGGAGATATAGTAGATCGTGGA AGCACTAATCTGGGAGTGAATCAGGCCGGCTTCACCTTACACTCTGCAATTTATGCTGCACGCCCGGACG TGAAGTGCGTCGTGCACATTCACACCCCAGCAGGGGCTGCGGTCTCTGCAATGAAATGTGGCCTCTTGCC AATCTCCCCGGAGGCGCTTTCCCTTGGAGAAGTGGCTTATCATGACTACCATGGCATTCTGGTTGATGAA GAGGAAAAAGTTTTGATTCAGAAAAATCTGGGGCCTAAAAGCAAGGTTCTTATTCTCCGGAACCATGGGC TCGTGTCAGTTGGAGAGAGCGTTGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCCTGTGAGAT CCAGGTTCGAACTCTGGCCAGTGCAGGAGGACCAGACAACTTAGTCCTGCTGAATCCTGAGAAGTACAAA GCCAAGTCCCGTTCCCCAGGGTCTCCGGTAGGGGAAGGCACTGGATCGCCTCCCAAGTGGCAGATTGGTG AGCAGGAATTTGAAGCCCTCATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATA CCCTGCTCTGAGAGAGAAGTCTAAAAAATACAGCGATGTGGAGGTTCCTGCTAGTGTCACAGGTTACTCC TTTGCTAGTGACGGTGATTCGGGCACTTGCTCCCCACTCAGACACAGTTTTCAGAAGCAGCAGCGGGAGA AGACAAGATGGCTGAACTCTGGCCGGGGCGACGAAGCTTCCGAGGAAGGGCAGAATGGAAGCAGTCCCAA GTCGAAGACTAAGGTGTGGACGAACATTACACACGATCACGTGAAACCCTTGCTGCAGTCTCTCTCGTCC GGTGTCTGCGTGCCAAGCTGTATTACCAACTGCTTGTGGACTAAAGAGGATGGACATAGAACTTCCACCT CTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGAT CCGAGAGCAGAATTTACAGGACATTAAGACGGCTGGCCCTCAGTCCCAGGTTTTGTGTGGTGTAGTGATG GACAGGAGCCTCGTCCAGGGAGAGCTGGTGACGGCCTCCAAGGCCATCATTGAAAAGGAGTACCAGCCCC ACGTCATTGTGAGCACCACGGGCCCCAACCCCTTCACCACACTCACAGACCGTGAGCTGGAGGAGTACCG CAGGGAGGTGGAGAGGAAGCAGAAGGGCTCTGAAGAGAATCTGGACGAGGCTAGAGAACAGAAAGAAAAG AGTCCTCCAGACCAGCCTGCGGTCCCCCACCCGCCTCCCAGCACTCCCATCAAGCTGGAGGAAGACCTTG TGCCGGAGCCGACTACTGGAGATGACAGTGATGCTGCCACCTTTAAGCCAACTCTCCCCGATCTGTCCCC TGATGAACCTTCAGAAGCACTCGGCTTCCCAATGTTAGAGAAGGAGGAGGAAGCCCATAGACCCCCAAGC CCCACTGAGGCCCCTACTGAGGCCAGCCCCGAGCCAGCCCCAGACCCAGCCCCGGTGGCTGAAGAGGCTG CCCCCTCAGCTGTCGAGGAGGGGGCCGCCGCGGACCCTGGCAGCGATGGGTCTCCAGGCAAGTCCCCGTC CAAAAAGAAGAAGAAGTTCCGTACCCCGTCCTTTCTGAAGAAGAGCAAGAAGAAGAGTGACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215898 representing NM_014189
Red=Cloning site Green=Tags(s) MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEE LESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDK GEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRG STNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDE EEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYK AKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYS FASDGDSGTCSPLRHSFQKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTKVWTNITHDHVKPLLQSLSS GVCVPSCITNCLWTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVM DRSLVQGELVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYRREVERKQKGSEENLDEAREQKEK SPPDQPAVPHPPPSTPIKLEEDLVPEPTTGDDSDAATFKPTLPDLSPDEPSEALGFPMLEKEEEAHRPPS PTEAPTEASPEPAPDPAPVAEEAAPSAVEEGAAADPGSDGSPGKSPSKKKKKFRTPSFLKKSKKKSDS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014189 |
| ORF Size | 2304 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014189.3 |
| RefSeq Size | 4063 bp |
| RefSeq ORF | 2307 bp |
| Locus ID | 118 |
| UniProt ID | P35611 |
| Domains | Aldolase_II |
| Protein Families | Druggable Genome |
| Gene Summary | Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215898 | ADD1 (Myc-DDK-tagged)-Human adducin 1 (alpha) (ADD1), transcript variant 2 |
CNY 8,080.00 |
|
| RC215898L3 | Lenti-ORF clone of ADD1 (Myc-DDK-tagged)-Human adducin 1 (alpha) (ADD1), transcript variant 2 |
CNY 9,980.00 |
|
| RC215898L4 | Lenti-ORF clone of ADD1 (mGFP-tagged)-Human adducin 1 (alpha) (ADD1), transcript variant 2 |
CNY 9,980.00 |
|
| SC115129 | ADD1 (untagged)-Human adducin 1 (alpha) (ADD1), transcript variant 2 |
CNY 14,820.00 |
其它alpha Adducin产品
