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PKN1 (NM_213560) Human Tagged ORF Clone
CAT＃: RG215735
- TrueORF®
PKN1 (tGFP-tagged) - Human protein kinase N1 (PKN1), transcript variant 1
ORF Plasmid: DDK
"NM_213560" in other vectors (6)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215735 representing NM_213560
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCCAATAACCCCTCGGAGCAGGAGCTGGAGAGTGAGCCTCGCAGCTGGTCCCTGCTAGAGC AGCTGGGCCTGGCCGGGGCAGACCTGGCGGCCCCCGGGGTACAGCAGCAGCTGGAGCTGGAGCGGGAGCG GCTGCGGCGGGAAATCCGCAAGGAGCTGAAGCTGAAGGAGGGTGCTGAGAACCTGCGGCGGGCCACCACT GACCTGGGCCGCAGCCTGGGCCCCGTAGAGCTGCTGCTGCGGGGCTCCTCGCGCCGCCTCGACCTGCTGC ACCAGCAGCTGCAGGAGCTGCACGCCCACGTGGTGCTTCCCGACCCGGCGGCCACCCACGATGGCCCCCA GTCCCCTGGTGCGGGTGGCCCCACCTGCTCGGCCACCAACCTGAGCCGCGTGGCGGGCCTGGAGAAGCAG TTGGCCATTGAGCTGAAGGTGAAGCAGGGGGCGGAGAACATGATCCAGACCTACAGCAATGGCAGCACCA AGGACCGGAAGCTGCTGCTGACAGCCCAGCAGATGTTGCAGGACAGTAAGACCAAGATTGACATCATCCG CATGCAACTCCGCCGGGCGCTGCAGGCCGGCCAGCTGGAGAACCAGGCAGCCCCGGATGACACCCAAGGG AGTCCTGACCTGGGGGCTGTGGAGCTGCGCATCGAAGAGCTGCGGCACCACTTCCGAGTGGAGCACGCGG TGGCCGAGGGTGCCAAGAACGTACTGCGCCTGCTCAGCGCTGCCAAGGCCCCGGACCGCAAGGCAGTCAG CGAGGCCCAGGAGAAATTGACAGAATCCAACCAGAAGCTGGGGCTGCTGCGGGAGGCTCTGGAGCGGAGA CTTGGGGAGCTGCCCGCCGACCACCCCAAGGGGCGGCTGCTGCGAGAAGAGCTCGCTGCGGCCTCCTCCG CTGCCTTCAGCACCCGCCTGGCCGGGCCCTTTCCCGCCACGCACTACAGCACCCTGTGCAAGCCCGCGCC GCTCACAGGGACCCTGGAGGTACGAGTGGTGGGCTGCAGAGACCTCCCAGAGACCATCCCGTGGAACCCT ACCCCCTCAATGGGGGGACCTGGGACCCCAGACAGCCGCCCCCCCTTCCTGAGCCGCCCAGCCCGGGGCC TTTACAGCCGAAGCGGAAGCCTCAGTGGCCGGAGCAGCCTCAAAGCAGAAGCCGAGAACACCAGTGAAGT CAGCACTGTGCTTAAGCTGGATAACACAGTGGTGGGGCAGACGTCTTGGAAGCCATGTGGCCCCAATGCC TGGGACCAGAGCTTCACTCTGGAGCTGGAAAGGGCACGGGAACTGGAGTTGGCTGTGTTCTGGCGGGACC AGCGGGGCCTGTGTGCCCTCAAATTCCTGAAGTTGGAGGATTTCTTGGACAATGAGAGGCATGAGGTGCA GCTGGACATGGAACCCCAGGGCTGCCTGGTGGCTGAGGTCACCTTCCGCAACCCTGTCATTGAGAGGATT CCTCGGCTCCGACGGCAGAAGAAAATTTTCTCCAAGCAGCAAGGGAAGGCGTTCCAGCGTGCTAGGCAGA TGAACATCGATGTCGCCACGTGGGTGCGGCTGCTCCGGAGGCTCATCCCCAATGCCACGGGCACAGGCAC CTTTAGCCCTGGGGCTTCTCCAGGATCCGAGGCCCGGACCACGGGTGACATATCGGTGGAGAAGCTGAAC CTCGGCACTGACTCGGACAGCTCACCTCAGAAGAGCTCGCGGGATCCTCCTTCCAGCCCATCGAGCCTGA GCTCCCCCATCCAGGAATCCACTGCTCCCGAGCTGCCTTCGGAGACCCAGGAGACCCCAGGCCCCGCCCT GTGCAGCCCTCTGAGGAAGTCACCTCTGACCCTCGAAGATTTCAAGTTCCTGGCGGTGCTGGGCCGGGGT CATTTTGGGAAGGTGCTCCTCTCCGAATTCCGGCCCAGTGGGGAGCTGTTCGCCATCAAGGCTCTGAAGA AAGGGGACATTGTGGCCCGAGACGAGGTGGAGAGCCTGATGTGTGAGAAGCGGATATTGGCGGCAGTGAC CAGTGCGGGACACCCCTTCCTGGTGAACCTCTTCGGCTGTTTCCAGACACCGGAGCACGTGTGCTTCGTG ATGGAGTACTCGGCCGGTGGGGACCTGATGCTGCACATCCACAGCGACGTGTTCTCTGAGCCCCGTGCCA TCTTTTATTCCGCCTGCGTGGTGCTGGGCCTACAGTTTCTTCACGAACACAAGATCGTCTACAGGGACCT GAAGTTGGACAATTTGCTCCTGGACACCGAGGGCTACGTCAAGATCGCAGACTTTGGCCTCTGCAAGGAG GGGATGGGCTATGGGGACCGGACCAGCACATTCTGTGGGACCCCGGAGTTCCTGGCCCCTGAGGTGCTGA CGGACACGTCGTACACGCGAGCTGTGGACTGGTGGGGACTGGGTGTGCTGCTCTACGAGATGCTGGTTGG CGAGTCCCCATTCCCAGGGGATGATGAGGAGGAGGTCTTCGACAGCATCGTCAACGACGAGGTTCGCTAC CCCCGCTTCCTGTCGGCCGAAGCCATCGGCATCATGAGAAGGCTGCTTCGGAGGAACCCAGAGCGGAGGC TGGGATCTAGCGAGAGAGATGCAGAAGATGTGAAGAAACAGCCCTTCTTCAGGACTCTGGGCTGGGAAGC CCTGTTGGCCCGGCGCCTGCCACCGCCCTTTGTGCCCACGCTGTCCGGCCGCACCGACGTCAGCAACTTC GACGAGGAGTTCACCGGGGAGGCCCCCACACTGAGCCCGCCCCGCGACGCGCGGCCCCTCACAGCCGCGG AGCAGGCAGCCTTCCTGGACTTCGACTTCGTGGCCGGGGGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215735 representing NM_213560
Red=Cloning site Green=Tags(s) MAEANNPSEQELESEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATT DLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQ LAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQG SPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERR LGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNP TPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNA WDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERI PRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLN LGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRG HFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFV MEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKE GMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRY PRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNF DEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_213560 |
| ORF Size | 2844 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_213560.1, NP_998725.1 |
| RefSeq Size | 2964 bp |
| RefSeq ORF | 2847 bp |
| Locus ID | 5585 |
| UniProt ID | Q16512 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215735 | PKN1 (Myc-DDK-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 10,416.00 |
|
| RC215735L1 | Lenti-ORF clone of PKN1 (Myc-DDK-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 12,816.00 |
|
| RC215735L2 | Lenti-ORF clone of PKN1 (mGFP-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 9,030.00 |
|
| RC215735L3 | Lenti-ORF clone of PKN1 (Myc-DDK-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 9,030.00 |
|
| RC215735L4 | Lenti-ORF clone of PKN1 (mGFP-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 9,030.00 |
|
| SC308656 | PKN1 (untagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 18,340.00 |
