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PCDHA4 (NM_018907) Human Tagged ORF Clone
CAT＃: RG214854
- TrueORF®
PCDHA4 (tGFP-tagged) - Human protocadherin alpha 4 (PCDHA4), transcript variant 1
ORF Plasmid: DDK
"NM_018907" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CNR1; CNRN1; CRNR1; PCDH-ALPHA4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214854 representing NM_018907
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTTCCTGGGGAAGCGGCCAGGAATCCCGGCGTCTGCTGCTCTTACTTCTTCTCCTCGCAGCCT GGGAGGCAGGGAACGGTCAGCTCCACTACTCGGTCTCCGAGGAGGCCAAACACGGCACCTTCGTGGGCCG CATCGCGCAGGACCTGGGACTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGTGGCGTCCAAGGGC CGCGGAGGCCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGG AGCTGTGCCGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTAGACAGGCCGCTGCAGGTTTT CCATGTGGACGTGGAGGTGAGGGACATTAACGATAACCCGCCGGTGTTCCCAGCAACACAAAAGAACCTG TCCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCCTCGGATGCAGATATCGGGG AGAACGCCCTGCTCACTTACAGACTGAGCCCAAATGAATACTTTTCTCTGGAAAAACCACCTGATGACGA GCTGGTAAAAGGTCTTGGGCTTATATTACGGAAATCTTTAGACAGAGAAGAAGCTCCGGAGATTTTTTTA GTGCTCACAGCCACTGATGGAGGCAAACCCGAGTTGACTGGCACCGTTCAGTTACTCATCACAGTACTGG ATGCCAATGACAATGCCCCAGCTTTTGACAGAACCATTTATAAGGTGAGATTACTAGAAAATGTTCCTAA TGGAACATTGGTAATTAAACTTAACGCCTCAGATTTAGACGAAGGATTGAATGGGGACATTGTTTATTCA TTCTCAAATGATATTTCGCCAAATGTGAAATCCAAGTTTCACATAGATCCAATTACTGGACAAATTATTG TAAAGGGATATATTGACTTTGAAGAAAGCAAATCCTATGAAATTATTGTAGAGGGCATTGATAAGGGACA GCTCCCACTTTCTGGCCATTGTAGAGTTATTGTGGAAGTAGAAGACAACAACGATAATGTCCCAGATTTG GAATTCAAGTCTTTATCACTTCCAATTAGAGAGGACGCTCCACTGGGTACAGTCATCGCCCTGATCAGCG TGTCCGACAAAGACATGGGTGTCAATGGGCTGGTCACCTGCTCCTTGACGTCCCACGTCCCCTTCAAGCT GGTGTCCACCTTCAAGAATTACTACTCGTTGGTGCTGGACAGTGCCCTGGACCGCGAGAGCGTGTCAGCC TATGAGCTGGTGGTGACCGCGCGAGACGGGGGCTCGCCTTCGCTGTGGGCCACGGCCAGTGTTTCTGTGG AGGTGGCTGATGTGAACGACAACGCTCCGGCGTTCGCGCAGCCCGAGTACACAGTGTTCGTGAAGGAGAA CAACCCGCCGGGCTGCCACATCTTCACTGTGTCTGCGTGGGACGCGGACGCGCAGGAGAACGCGCTGGTG TCCTACTCGCTGGTAGAGCGGCGGGTAGGGGAGCGCGCGCTGTCGAGCTACGTTTCGGTGCATGCGGAGA GCGGCAAGGTGTACGCGCTGCAGCCGCTGGACCACGAGGAGCTAGAGCTGCTGCAGTTTCAGGTGACCGC TCGCGATGCCGGCGTGCCACCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAAAACGAC AACGCGCCAGCACTGCTAGCGCCTCGGGCGGGTGGCACTGGTGGCGCAGTGAGCGAGCTGGTGCCATGGT CGGTGGGTGTGGGCCACGTGGTGGCAAAGGTGCGCGCGGTGGATGCTGACTCGGGCTACAACGCGTGGCT TTCGTACGAGCTGCAGCCGGGGACTGGTGGCGCGCGCATCCCGTTCCGCGTGGGGCTGTACACTGGCGAG ATCAGCACAACGCGTGCCCTGGACGAAACGGACGCTCCGCGCCACCGCCTACTGGTACTGGTGAAGGACC ACGGCGAGCCCGCGCTGACGGCCACGGCCACTGTGCTGGTGTCACTTGTGGAGAGTGGACAGGCGCCAAA GGCCTCCTCACGGGCGTTGGTGGGCGCTGTGGGTCCCGATGCTGCGCTGGTGGATGTCAACGTATACCTG ATCATTGCCATCTGCGCGGTGTCCAGCCTTTTGGTGCTCACGCTGCTGCTGTACACCGCGCTGCGGTGCT CTGCGCTGCCCACCGAGGGCGCGTGCGCTCCGGGCAAGCCCACGCTGGTGTGCTCCAGTGCGGTGGGGAG CTGGTCATACTCGCAGCAGAGGAGGCCGAGGGTGTGCTCTGGTGAGGGCCCACCCAAGACCGACCTCATG GCCTTCAGCCCCAGTTTACCTGACTCTAGGGACAGAGAAGATCAGCTGCAGACAACTGAGGAATCCTTTG CAAAGCCACGACAGCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGT GCACCTAGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCC AGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCGGGTGTCAACAGCAACAGCT GGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGTCCCGGTGAGTTGCCCGACAAATTCATTAT CCCAGGATCTCCTGCAATCATCTCCATCCGGCAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTC ATAACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGA AAAAAGAGAAAGGGAACAGCACGACTGACAACAGTGACCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214854 representing NM_018907
Red=Cloning site Green=Tags(s) MEFSWGSGQESRRLLLLLLLLAAWEAGNGQLHYSVSEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKG RGGLLEVNLQNGILFVNSRIDREELCRRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPVFPATQKNL SIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFSLEKPPDDELVKGLGLILRKSLDREEAPEIFL VLTATDGGKPELTGTVQLLITVLDANDNAPAFDRTIYKVRLLENVPNGTLVIKLNASDLDEGLNGDIVYS FSNDISPNVKSKFHIDPITGQIIVKGYIDFEESKSYEIIVEGIDKGQLPLSGHCRVIVEVEDNNDNVPDL EFKSLSLPIREDAPLGTVIALISVSDKDMGVNGLVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRESVSA YELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAWDADAQENALV SYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVTARDAGVPPLGSNVTLQVFVLDEND NAPALLAPRAGGTGGAVSELVPWSVGVGHVVAKVRAVDADSGYNAWLSYELQPGTGGARIPFRVGLYTGE ISTTRALDETDAPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRALVGAVGPDAALVDVNVYL IIAICAVSSLLVLTLLLYTALRCSALPTEGACAPGKPTLVCSSAVGSWSYSQQRRPRVCSGEGPPKTDLM AFSPSLPDSRDREDQLQTTEESFAKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVS SATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDF ITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018907 |
| ORF Size | 2841 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018907.4 |
| RefSeq Size | 5352 bp |
| RefSeq ORF | 2844 bp |
| Locus ID | 56144 |
| UniProt ID | Q9UN74 |
| Domains | CA |
| Protein Families | Transmembrane |
| Gene Summary | This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214854 | PCDHA4 (Myc-DDK-tagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 1 |
CNY 7,130.00 |
|
| RC214854L3 | Lenti-ORF clone of PCDHA4 (Myc-DDK-tagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 1 |
CNY 9,030.00 |
|
| RC214854L4 | Lenti-ORF clone of PCDHA4 (mGFP-tagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 1 |
CNY 9,030.00 |
|
| SC314971 | PCDHA4 (untagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 1 |
CNY 6,944.00 |
