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RAD17 (NM_133344) Human Tagged ORF Clone
CAT＃: RG214722
- TrueORF®
RAD17 (tGFP-tagged) - Human RAD17 homolog (S. pombe) (RAD17), transcript variant 7
ORF Plasmid: DDK
"NM_133344" in other vectors (6)
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CNY 6,512.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CCYC; HRAD17; R24L; RAD17SP; RAD24 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214722 representing NM_133344
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGCGTCTCTACTA TTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAAAG CAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCTTCCTTAGAACAGATTTATGGTTTAGAAAATTCA AAAGAATATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCAGCATGAACTTGCTG TGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAAAACA GGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATACTATCA AAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAAAGATGATTTCA AGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTTTTCAAAGA GTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGATCTGAGAACTGATAAGAAG ATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAAGTTCTAAGGA AGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGAGATAATAATCA AAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTCAACCCTGTGGCA CCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAATGGAGGAAAAATTA CTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATCAGAAGTGCAATAAA CAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAAAAAGGAATGTCTTTAAAA TCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGGGTTTTTGAAAATCAAGAGGTCC AAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGGGGAAAATTCTATATTGTAAAAG AGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATCAGAATATGAACGGGATACATTA CTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTATTTAATTTATATCTTCACC AAAACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGTGAATTTCTGAGTTTTGCAGA TATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGCACATCTATAGCTACGAGAGGT GTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGAGGATCAAGTTTTCGACCCTTGC ACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGCCTGGCAGCAAAAGCACTTTTTCC TGACTTCTGCCTACCAGCTTTATGCCTCCAAACTCAGCTATTGCCATACCTTGCTCTACTAACCATTCCA ATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGGCTCCCTCTGAAGCGACACTTTGGAA GATTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATAGACCCTGACAGCGGAGATGAAGCCCA GCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCCACTCAAGCCACTGTGCCGGAAACCTGG TCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCTAGCCAGCCCCAGCCCTTTTCAGCCCAAG GAGACATGGAAGAAAACATAATAATAGAAGACTACGAGAGTGATGGGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214722 representing NM_133344
Red=Cloning site Green=Tags(s) MNQVTDWVDPSFDDFLECSGVSTITATSLGVNNSSHRRKNGPSTLESSRFPARKRGNLSSLEQIYGLENS KEYLSENEPWVDKYKPETQHELAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILS KEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAVFKEFLLRATKYNKLQMLGDDLRTDKK IILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVA PTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLK SDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYERDTL LVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDWNTRSLLREYSTSIATRG VMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCLQTQLLPYLALLTIP MRNQAQISFIQDIGRLPLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEPTQATVPETW SLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133344 |
| ORF Size | 2010 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133344.3 |
| RefSeq Size | 2842 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 5884 |
| UniProt ID | O75943 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214722 | RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 7 |
CNY 4,912.00 |
|
| RC214722L1 | Lenti ORF clone of Human RAD17 homolog (S. pombe) (RAD17), transcript variant 7, Myc-DDK-tagged |
CNY 7,312.00 |
|
| RC214722L2 | Lenti ORF clone of Human RAD17 homolog (S. pombe) (RAD17), transcript variant 7, mGFP tagged |
CNY 5,890.00 |
|
| RC214722L3 | Lenti ORF clone of Human RAD17 homolog (S. pombe) (RAD17), transcript variant 7, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214722L4 | Lenti ORF clone of Human RAD17 homolog (S. pombe) (RAD17), transcript variant 7, mGFP tagged |
CNY 5,890.00 |
|
| SC124514 | RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 7 |
CNY 4,912.00 |
