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Myosin 8 (MYH8) (NM_002472) Human Tagged ORF Clone
CAT＃: RG214641
- TrueORF®
MYH8 (tGFP-tagged) - Human myosin, heavy chain 8, skeletal muscle, perinatal (MYH8)
ORF Plasmid: DDK
"NM_002472" in other vectors (2)
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CNY 15,580.00
货期*
8周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DA7; gtMHC-F; MyHC-peri; MyHC-pn |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214641 representing NM_002472
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCGAGCTCAGACGCTGAGATGGCTGTTTTTGGCGAAGCTGCTCCCTACCTTCGAAAATCAGAAA AGGAGCGGATTGAGGCCCAAAACAAGCCGTTTGATGCTAAAACATCTGTCTTTGTGGCGGAGCCCAAGGA ATCCTATGTGAAGAGCACTATACAAAGCAAAGAAGGAGGGAAAGTAACCGTAAAGACTGAAGGTGGAGCA ACTCTAACTGTCAGGGAAGACCAAGTCTTCCCTATGAACCCTCCGAAATATGACAAAATTGAGGACATGG CCATGATGACTCATCTACACGAGCCTGGAGTGCTGTACAACCTCAAAGAGCGCTATGCAGCCTGGATGAT CTACACCTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAAGCCCGAG GTGGTGGCTGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAATG CCTATCAGTTCATGTTGACTGATCGAGAGAATCAGTCCATCCTGATCACCGGAGAATCTGGTGCCGGAAA GACTGTGAACACCAAGCGTGTCATCCAATACTTTGCAACAATTGCAGTTACTGGAGAGAAGAAGAAGGAT GAATCTGGCAAAATGCAGGGGACTCTGGAAGATCAAATCATCAGCGCCAATCCCCTACTGGAGGCCTTTG GCAATGCCAAAACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATTAGAATCCACTTTGGTAC TACAGGGAAGCTGGCATCTGCTGATATAGAAACATATCTTTTAGAAAAGTCCAGAGTTACTTTCCAGCTA AAGGCGGAAAGAAGCTACCATATTTTTTATCAGATCACTTCCAATAAGAAGCCAGATCTAATTGAAATGC TCCTGATCACCACCAACCCATATGACTATGCCTTCGTCAGTCAGGGGGAGATCACAGTTCCCAGTATTGA TGACCAAGAAGAGTTGATGGCCACTGATAGTGCCATTGACATCCTGGGCTTCACTCCTGAAGAGAAAGTG TCCATCTATAAACTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAGCGTGAGG AGCAAGCTGAGCCAGATGGCACAGAAGTCGCTGACAAGGCAGCCTATCTCCAGAGTCTGAACTCTGCAGA CCTACTCAAAGCCCTCTGCTACCCTAGGGTCAAGGTTGGCAATGAGTATGTCACCAAAGGCCAGACTGTG CAGCAGGTGTACAATGCCGTGGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG CTTTGAAATCTTTGATTTTAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTTGTGCTAGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCA TTGACTTTGGGATGGACCTGGCTGCCTGCATTGAGCTCATTGAGAAGCCACTGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCTAAGGCCACGGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTG GGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAGGCTGAGGCCCACTTCTCTCTGATTC ACTATGCTGGCACTGTGGACTACAACATTACTGGCTGGCTGGACAAAAATAAGGACCCCCTGAATGATAC TGTGGTTGGGCTGTACCAGAAGTCTGCAATGAAGACTCTAGCCAGTCTCTTTTCCACGTATGCTAGTGCT GAAGCAGATAGCAGCGCGAAGAAAGGTGCTAAGAAAAAGGGCTCTTCTTTCCAGACTGTGTCTGCCCTTT TCAGGGAAAATTTAAATAAATTGATGACGAATCTGAGGAGCACACACCCTCACTTCGTACGGTGTATCAT TCCCAATGAAACCAAAACTCCTGGGGCAATGGAACATGAACTTGTGTTGCACCAGCTGAGGTGTAATGGT GTGCTGGAAGGCATCCGCATCTGTAGGAAAGGATTCCCAAGCAGAATCTTATATGGTGATTTCAAACAAA GATACAAGGTTTTAAATGCAAGTGCTATTCCAGAGGGACAGTTCATTGACAGCAAGAAGGCTTCTGAGAA ACTTCTTGCATCTATTGATATTGATCATACTCAATATAAATTTGGACATACCAAGGTTTTCTTCAAAGCT GGACTTCTGGGTCTTCTGGAAGAAATGAGAGATGAAAAATTAGCCCAAATTATAACAAGAACACAAGCTG TCTGTAGGGGATTCCTAATGAGGGTAGAATATCAGAAGATGTTGCAAAGGAGAGAAGCACTTTTCTGCAT CCAGTATAATGTCCGTGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTTTTCAAGATTAAG CCCCTCCTCAAGAGTGCAGAGACCGAGAAAGAGATGGCCACCATGAAGGAAGAATTCCAGAAAACCAAAG ATGAACTCGCCAAGTCAGAGGCAAAACGGAAGGAGCTAGAGGAAAAAATGGTCACTCTCTTAAAAGAGAA AAATGACCTGCAACTCCAGGTTCAATCTGAAGCAGATAGCTTGGCTGATGCAGAGGAAAGGTGTGAGCAA CTGATTAAAAACAAAATCCAACTTGAGGCCAAAATCAAAGAGGTGACTGAAAGAGCTGAGGAGGAGGAAG AGATCAATGCTGAGCTGACAGCCAAGAAGAGAAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGACAT TGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGCAGAAACATGCCACGGAGAACAAGGTGAAAAAT CTTACAGAAGAGATGGCAGGCCTGGATGAAACCATTGCAAAACTGTCCAAGGAGAAGAAGGCTCTCCAAG AGACCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACATCCTGACCAAAGCTAA AACCAAGCTAGAACAGCAAGTGGATGATCTTGAAGGGTCTCTGGAACAAGAAAAGAAGCTTCGAATGGAT CTAGAAAGAGCAAAGCGGAAACTGGAGGGTGACCTCAAATTGGCCCAAGAATCCACAATGGATATGGAAA ATGACAAACAGCAACTTGATGAAAAGCTTGAAAAGAAAGAATTTGAAATCAGCAATTTGATAAGCAAAAT TGAAGATGAGCAAGCTGTAGAAATTCAACTACAGAAGAAGATCAAAGAGTTGCAGGCCCGCATTGAGGAG CTGGGGGAAGAAATCGAGGCAGAGAGGGCGTCCCGAGCCAAAGCGGAGAAGCAGCGCTCTGACCTCTCCC GGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGTGGGGCAACTTCTGCTCAGGTGGAATTGAA CAAGAAGCGGGAGGCTGAGTTTCAGAAACTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCATGAAGCT ATGGTGGCTGCTCTTCGGAAGAAGCACGCAGACAGTATGGCTGAGCTTGGGGAGCAGATTGACAACTTGC AGCGGGTCAAACAGAAGCTGGAGAAGGAGAAGAGTGAGCTGAAGATGGAGACTGATGACCTCAGCAGTAA CGCAGAGGCCATTTCCAAAGCCAAGGGCCACCTTGAAAAGATGTGCCGCTCTCTAGAAGATCAAGTGAGT GGGCTTAAGACCAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGAGCGCGCCTGC AGACAGAAGCGGGTGAATATTCTCGACAATTAGATGAGCAAGATGCTTTAGTCTCTCAGCTTTCAAGGAG CAAGCAAGCATCTACTCAGCAGATTGAAGAGCTGAAACATCAACTAGAGGAAGAAACTAAAGCCAAGAAC GCCCTGGCACACGCCCTGCAGTCCTCCCGCCATGACTGCGACCTGCTGCGGGAACAGTATGAGGAAGAGC AGGAAGGCAAAGCTGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGAACCAA ATACGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAAAAGTTGGCCCAGCGCCTG CAAGAAGCTGAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTTGAGAAGACGAAGCAGCGGC TCCAGAATGAAGTTGAAGACCTCATGCTTGATGTGGAAAGGTCTAATGCAGCCTGTGCAGCCCTTGATAA GAAGCAAAGGAACTTTGACAAGGTCCTATCAGAATGGAAGCAGAAGTATGAGGAAACTCAGGCTGAACTT GAGGCCTCCCAGAAGGAGTCACGTTCTCTTAGCACTGAGCTGTTCAAGGTGAAGAATGTCTATGAGGAAT CCCTGGATCAACTCGAAACGCTAAGAAGAGCACATAAGAACTTGCAACAGGAGATTTCTGACCTCACTGA GCAGATTGCAGAGGGAGGAAAGCAAATTCATGAATTGGAGAAAATAAAGAAGCAAGTAGAACAAGAGAAA TGTGAAATTCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTTGAACATGAAGAAGGAAAGATTCTGCGTA TCCAGCTTGAGTTAAACCAAGTCAAGTCTGAAGTTGATAGAAAAATCGCAGAAAAGGATGAGGAAATTGA CCAGCTGAAGAGAAACCACACTAGAGTCGTGGAGACAATGCAGAGCACGCTGGATGCAGAGATTAGAAGC AGAAATGATGCTCTGAGAGTCAAGAAGAAAATGGAAGGAGATCTGAATGAAATGGAAATCCAGCTGAACC ATGCCAATCGCTTAGCTGCAGAGAGTTTAAGGAACTACAGGAACACCCAAGGAATCCTGAAGGAAACCCA GCTCCACCTGGATGATGCTCTCCGGGGCCAGGAGGACCTCAAGGAACAGCTGGCAATTGTGGAGCGCAGA GCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGTGGGCCACTCTGGAACAGACAGAGAGAAGCAGGAAAA TCGCCGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTCCAGCTCCTCCACACCCAGAATACCAGTCTCAT TAACACCAAGAAGAAATTAGAAAATGACGTTTCCCAACTCCAAAGTGAAGTGGAAGAAGTAATCCAAGAA TCACGCAATGCAGAAGAGAAAGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGAAGA AGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCA GCATCGTCTAGATGAGGCCGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAGGCC AGGGTACGTGAGCTTGAAGGAGAGGTTGAAAATGAACAGAAACGTAATGCAGAGGCTGTTAAAGGTTTAC GGAAACATGACCGACGAGTAAAAGAACTCACCTACCAGACTGAAGAAGATCGCAAGAATGTTCTCAGGCT GCAGGACTTGGTAGATAAATTACAGGCGAAGGTGAAATCATACAAGAGACAAGCTGAGGAGGCTGAGGAA CAATCCAATGCTAATCTATCTAAATTCCGCAAACTCCAGCATGAGCTGGAGGAGGCCGAGGAACGGGCTG ACATTGCTGAGTCCCAGGTCAACAAATTGCGAGTGAAGAGCCGAGAGGTTCACACAAAAATCAGTGCAGA G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214641 representing NM_002472
Red=Cloning site Green=Tags(s) MSASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAEPKESYVKSTIQSKEGGKVTVKTEGGA TLTVREDQVFPMNPPKYDKIEDMAMMTHLHEPGVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPE VVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKD ESGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL KAERSYHIFYQITSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTPEEKV SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTV QQVYNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHL GKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNDTVVGLYQKSAMKTLASLFSTYASA EADSSAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNG VLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKA GLLGLLEEMRDEKLAQIITRTQAVCRGFLMRVEYQKMLQRREALFCIQYNVRAFMNVKHWPWMKLFFKIK PLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQSEADSLADAEERCEQ LIKNKIQLEAKIKEVTERAEEEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKQKHATENKVKN LTEEMAGLDETIAKLSKEKKALQETHQQTLDDLQAEEDKVNILTKAKTKLEQQVDDLEGSLEQEKKLRMD LERAKRKLEGDLKLAQESTMDMENDKQQLDEKLEKKEFEISNLISKIEDEQAVEIQLQKKIKELQARIEE LGEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQVELNKKREAEFQKLRRDLEEATLQHEA MVAALRKKHADSMAELGEQIDNLQRVKQKLEKEKSELKMETDDLSSNAEAISKAKGHLEKMCRSLEDQVS GLKTKEEEQQRLINDLTAQRARLQTEAGEYSRQLDEQDALVSQLSRSKQASTQQIEELKHQLEEETKAKN ALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRL QEAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERSNAACAALDKKQRNFDKVLSEWKQKYEETQAEL EASQKESRSLSTELFKVKNVYEESLDQLETLRRAHKNLQQEISDLTEQIAEGGKQIHELEKIKKQVEQEK CEIQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHTRVVETMQSTLDAEIRS RNDALRVKKKMEGDLNEMEIQLNHANRLAAESLRNYRNTQGILKETQLHLDDALRGQEDLKEQLAIVERR ANLLQAEIEELWATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLENDVSQLQSEVEEVIQE SRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEA RVRELEGEVENEQKRNAEAVKGLRKHDRRVKELTYQTEEDRKNVLRLQDLVDKLQAKVKSYKRQAEEAEE QSNANLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKISAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002472 |
| ORF Size | 5811 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002472.1, NP_002463.1 |
| RefSeq Size | 6016 bp |
| RefSeq ORF | 5814 bp |
| Locus ID | 4626 |
| UniProt ID | P13535 |
| Protein Pathways | Tight junction, Viral myocarditis |
| Gene Summary | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009] |
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