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SRGAP3 (NM_014850) Human Tagged ORF Clone
CAT＃: RG214288
- TrueORF®
SRGAP3 (tGFP-tagged) - Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 1
ORF Plasmid: DDK
"NM_014850" in other vectors (4)
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CNY 11,970.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARHGAP14; MEGAP; SRGAP2; WRP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214288 representing NM_014850
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGA TCCGCACGCAGCTGGTGGAGCAGTTCAAATGTCTGGAGCAGCAATCAGAGTCGCGACTGCAGCTGCTTCA AGACCTCCAGGAGTTTTTCCGCCGGAAAGCTGAGATTGAGCTCGAGTACTCCCGCAGCCTGGAGAAGCTG GCTGAGCGCTTCTCCTCCAAAATCCGCAGCTCCCGGGAGCACCAGTTCAAGAAGGACCAGTACCTCCTCT CGCCTGTGAACTGTTGGTATCTGGTTCTGCATCAGACCCGGCGGGAGAGCCGAGACCATGCCACCCTCAA TGACATCTTCATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAGGATGTCATCAGACTCTTCAAA AAGAGCAAGGAGATTGGCCTGCAGATGCACGAGGAGCTCCTGAAGGTGACCAATGAGCTCTACACAGTCA TGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCGGAAAGCAAGCTGAAGGAGGCTGAGAAGCA GGAGGAGAAGCAGTTCAATAAGTCAGGAGACCTCAGCATGAACCTGCTCCGGCACGAGGACCGGCCCCAG CGCCGCAGCTCTGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTACTCTGAGAACAAGC TGAAATGCACAAAGGCCCGGAATGACTACTTGCTCAATCTGGCAGCCACCAACGCAGCTATAAGCAAATA CTACATCCATGATGTCTCTGATCTGATCGATTGCTGTGATTTGGGCTTCCATGCCAGCCTGGCCCGCACC TTCCGGACCTATCTCTCAGCTGAATACAACCTGGAGACCTCTCGCCACGAAGGGCTGGATGTCATTGAGA ATGCAGTGGACAACCTGGATTCCCGAAGTGACAAGCACACAGTCATGGACATGTGCAATCAAGTCTTCTG CCCTCCACTCAAGTTCGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGCGCTCAGCAGCCC GTCCAGACAGAACTGCTCATGCGTTATCACCAGCTGCAGTCCAGACTGGCCACCCTCAAGATAGAGAATG AGGAGGTTAGGAAAACCCTGGATGCCACCATGCAGACATTACAGGACATGCTGACTGTGGAGGACTTTGA TGTCTCCGATGCCTTCCAACACAGTCGATCGACAGAGTCCGTCAAGTCGGCTGCCTCTGAGACCTACATG AGCAAGATCAACATTGCCAAGAGGAGAGCCAACCAGCAGGAAACAGAAATGTTTTATTTTACAAAATTTA AAGAGTATGTGAATGGCAGTAACCTCATCACCAAGCTGCAGGCCAAGCACGATTTACTCAAGCAGACCCT GGGCGAAGGGGAAAGAGCAGAATGCGGCACCACCAGGCCCCCCTGTCTTCCCCCTAAACCACAGAAAATG AGGAGACCTAGGCCTCTCTCAGTGTATAGCCATAAACTCTTTAACGGCAGTATGGAAGCATTCATTAAGG ATTCAGGACAAGCTATACCGCTTGTAGTCGAGAGCTGCATCCGTTACATCAATTTATATGGACTCCAGCA GCAGGGGATCTTCAGAGTGCCAGGATCTCAGGTGGAAGTCAATGACATCAAAAATTCCTTTGAGAGAGGT GAAGACCCCCTTGTGGACGATCAAAATGAACGAGATATCAATTCAGTCGCTGGTGTTTTAAAACTGTATT TCCGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGA GAACCCAGCCGAGAGGGTGCACCAGATCCAACAAATCCTCGTCACCCTTCCCCGCGTGGTCATTGTGGTC ATGAGATACCTCTTCGCTTTCCTCAACCACCTCTCCCAGTATAGCGACGAGAACATGATGGATCCCTACA ACCTGGCCATCTGCTTCGGGCCTACCCTCATGCACATCCCTGATGGGCAGGACCCTGTGTCCTGCCAGGC ACACATCAATGAAGTCATCAAAACCATCATCATCCATCATGAAGCCATCTTCCCCAGCCCCCGGGAGCTA GAGGGACCTGTGTATGAAAAATGCATGGCTGGAGGGGAAGAATATTGTGACAGCCCACACAGCGAGCCAG GGGCCATCGATGAAGTTGACCATGACAATGGCACTGAGCCTCATACCAGCGATGAAGAAGTGGAGCAGAT CGAGGCTATTGCCAAGTTTGACTACATGGGGCGGTCCCCGCGTGAGCTATCCTTCAAGAAGGGGGCCTCG CTGCTCCTGTACCACCGCGCCTCGGAGGACTGGTGGGAGGGCCGGCACAACGGCGTGGATGGACTCATCC CCCATCAGTACATAGTTGTACAGGACATGGATGATGCCTTCTCCGACAGCCTGAGCCAGAAGGCTGACAG CGAGGCCAGCAGTGGGCCATTGCTGGATGACAAGGCCTCTTCCAAAAACGACCTCCAGTCCCCCACGGAG CACATCTCGGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGCGGTTACGATCTGATGGAGCAGCCATCC CCAGACGCCGAAGCGGGGGCGACACACACAGCCCGCCCCGGGGCCTGGGCCCCAGCATAGACACACCACC CCGGGCTGCTGCCTGCCCCAGCAGCCCCCACAAAATCCCCCTCACCCGGGGGAGGATCGAGAGCCCTGAG AAGCGGAGGATGGCGACGTTCGGGAGCGCTGGCAGCATCAACTACCCTGACAAGAAGGCGCTCTCCGAAG GGCACTCGATGAGGTCGACCTGCGGTTCCACCAGGCACAGCAGCCTAGGGGACCACAAGTCCCTGGAGGC CGAGGCCCTGGCAGAAGACATCGAGAAGACCATGAGCACGGCTCTGCACGAGTTGCGGGAACTCGAGAGG CAGAACACGGTCAAGCAGGCGCCAGATGTGGTGCTGGACACCCTGGAGCCCCTGAAGAACCCGCCAGGCC CCGTCAGCTCGGAGCCCGCCAGTCCCCTTCACACCATCGTCATCCGCGACCCCGATGCCGCCATGCGCCG CAGCAGCAGCTCCTCCACCGAGATGATGACCACCTTCAAGCCAGCCCTGTCCGCCCGCCTGGCTGGCGCC CAGCTCCGCCCGCCCCCCATGCGGCCCGTGCGGCCGGTGGTCCAGCACCGGTCCAGCAGCAGCAGCAGCT CGGGCGTGGGCAGCCCGGCCGTGACGCCCACCGAGAAGATGTTCCCCAACAGCTCAGCGGACAAGTCGGG CACCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214288 representing NM_014850
Red=Cloning site Green=Tags(s) MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKL AERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFK KSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQ RRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLART FRTYLSAEYNLETSRHEGLDVIENAVDNLDSRSDKHTVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQP VQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESVKSAASETYM SKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKM RRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRYINLYGLQQQGIFRVPGSQVEVNDIKNSFERG EDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPAERVHQIQQILVTLPRVVIVV MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHINEVIKTIIIHHEAIFPSPREL EGPVYEKCMAGGEEYCDSPHSEPGAIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYMGRSPRELSFKKGAS LLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTE HISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLTRGRIESPE KRRMATFGSAGSINYPDKKALSEGHSMRSTCGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELER QNTVKQAPDVVLDTLEPLKNPPGPVSSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGA QLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPNSSADKSGTM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014850 |
| ORF Size | 3297 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014850.4 |
| RefSeq Size | 8901 bp |
| RefSeq ORF | 3300 bp |
| Locus ID | 9901 |
| UniProt ID | O43295 |
| Domains | RhoGAP, FCH, SH3 |
| Protein Pathways | Axon guidance |
| Gene Summary | GTPase-activating protein for RAC1 and perhaps Cdc42, but not for RhoA small GTPase. May attenuate RAC1 signaling in neurons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214288 | SRGAP3 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 1 |
CNY 7,656.00 |
|
| RC214288L3 | Lenti ORF clone of Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 1, Myc-DDK-tagged |
CNY 12,830.00 |
|
| RC214288L4 | Lenti ORF clone of Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 1, mGFP tagged |
CNY 12,830.00 |
|
| SC309984 | SRGAP3 (untagged)-Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 1 |
CNY 7,656.00 |
