SLC12A6 (NM_001042497) Human Tagged ORF Clone
CAT#: RG214194
- TrueORF®
SLC12A6 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 6
ORF Plasmid: DDK
"NM_001042497" in other vectors (4)
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CNY 12,450.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ACCPN; KCC3; KCC3A; KCC3B |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214194 representing NM_001042497
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCCTCCAGAAACCACCACCAAGATGGCTTCAGTTCGGTTCATGGTGACACCGACAAAGATCGATG ACATTCCAGGTTTGTCAGACACCAGTCCGGACCTCAGCTCTCGATCTAGTTCCCGAGTAAGATTTAGCTC CCGGGAAAGCGTGCCTGAAACAAGCCGGAGTGAGCCTATGAGTGAGATGTCTGGGGCCACCACTTCGCTG GCAACTGTTGCACTGGATCCACCCAGTGACCGGACTTCTCACCCCCAGGATGTCATCGAGGACGACGGAC ATAAGAAAGCTCGAAATGCTTATCTCAATAATTCCAATTATGAAGAAGGAGATGAATATTTTGATAAAAA TTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCGAAGGTGTCTTCCCTCCTCAACCGCATGGCCAAT TACACTAATCTGACTCAAGGAGCAAAGGAACATGAAGAGGCAGAAAACATCACTGAAGGGAAAAAGAAGC CCACCAAGACCCCCCAAATGGGTACCTTCATGGGTGTCTACCTCCCATGTCTACAAAATATTTTTGGAGT GATCCTTTTTTTACGCCTTACATGGGTGGTGGGCACAGCTGGAGTTCTTCAGGCTTTTGCAATTGTCCTT ATCTGCTGCTGCTGTACAATGTTGACTGCTATCTCCATGAGTGCCATTGCCACTAATGGAGTGGTGCCAG CTGGGGGCTCATACTTTATGATTTCCCGGGCACTGGGCCCAGAGTTTGGTGGGGCTGTTGGCCTCTGCTT TTATCTTGGTACCACATTTGCAGCAGCCATGTACATCCTTGGTGCCATTGAAATCTTTCTGGTCTATATC GTCCCCCGAGCTGCCATCTTTCACAGTGATGACGCACTCAAGGAATCAGCAGCCATGCTAAATAACATGC GTGTCTACGGCACAGCTTTCTTGGTCCTTATGGTATTAGTGGTATTTATCGGCGTACGCTATGTGAACAA GTTTGCCTCACTTTTCCTGGCCTGTGTCATTGTGTCCATCTTGGCCATCTATGCTGGAGCCATCAAGTCT TCTTTTGCTCCTCCACACTTCCCGGTCTGCATGCTGGGTAACCGCACCCTTTCATCAAGACACATTGACG TTTGCTCTAAGACCAAGGAAATTAACAACATGACAGTCCCATCAAAGTTATGGGGATTCTTCTGTAACTC GAGTCAATTTTTCAATGCCACCTGTGATGAATACTTTGTTCACAATAACGTCACTTCAATCCAGGGCATT CCTGGATTGGCTAGTGGTATAATTACAGAGAATCTTTGGAGTAATTACCTACCCAAGGGAGAGATCATCG AAAAGCCTTCAGCCAAATCTTCTGATGTCTTAGGCAGCTTAAACCATGAATATGTTCTTGTTGACATCAC CACCTCCTTCACGCTTCTGGTGGGAATCTTCTTTCCCTCTGTTACAGGTATCATGGCTGGATCAAACAGA TCTGGAGATCTGAAAGATGCTCAGAAGTCTATTCCGATTGGTACTATCCTTGCCATCCTGACCACCTCCT TTGTTTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATTGAAGGGGTTGTTCTCAGAGACAAGTTCGG TGATGCTGTGAAAGGTAATTTGGTGGTAGGCACCTTATCTTGGCCATCCCCATGGGTGATTGTTATTGGC TCCTTCTTTTCAACATGTGGGGCTGGACTTCAGAGCCTCACAGGTGCACCGAGGCTGCTACAAGCTATTG CCAAGGATAACATCATACCGTTTCTGAGGGTTTTTGGCCACAGCAAAGCCAATGGGGAACCTACCTGGGC TTTACTTCTAACTGCTGCCATTGCAGAGCTTGGAATACTCATTGCCTCCCTGGATCTTGTGGCCCCAATT CTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTAAACTTGGCATGTGCCTTGCAAACATTACTTCGAA CACCCAACTGGAGACCCCGATTCCGCTACTACCATTGGGCCCTTTCTTTCATGGGAATGAGTATCTGTCT GGCTCTGATGTTCATTTCTTCCTGGTATTATGCCATTGTAGCCATGGTAATAGCTGGTATGATCTACAAG TACATTGAATACCAAGGAGCTGAGAAAGAATGGGGTGATGGTATCCGTGGGCTGTCCCTCAGTGCAGCCC GGTTTGCTTTGCTTCGATTGGAGGAAGGACCTCCACACACTAAAAACTGGAGGCCTCAGTTGCTTGTATT ACTGAAACTAGATGAAGACTTACATGTCAAGCATCCTCGCCTCCTCACCTTTGCCTCACAGCTCAAAGCA GGAAAAGGTCTCACTATTGTGGGCTCTGTCATCGTGGGGAACTTCCTAGAGAACTACGGTGAAGCTTTAG CTGCTGAGCAGACCATAAAGCACCTAATGGAGGCAGAGAAGGTAAAAGGATTCTGCCAGCTGGTGGTGGC CGCCAAGCTGAGAGAGGGCATTTCCCACCTCATCCAGTCATGTGGCCTTGGGGGCATGAAGCACAACACG GTGGTGATGGGCTGGCCTAATGGCTGGCGTCAAAGCGAAGATGCCCGCGCTTGGAAGACTTTTATTGGCA CAGTTCGAGTGACAACTGCTGCCCATCTTGCACTGCTGGTGGCTAAAAACATCTCCTTCTTTCCCAGCAA TGTGGAGCAATTTTCTGAGGGCAACATTGATGTGTGGTGGATTGTGCATGATGGGGGGATGCTTATGCTA CTACCATTCCTACTGAAACAGCACAAGGTGTGGCGAAAGTGCAGCATACGGATCTTCACAGTAGCCCAAT TAGAAGACAACAGTATCCAAATGAAGAAGGACCTAGCCACCTTCCTATATCACTTACGCATTGAGGCGGA GGTAGAAGTGGTGGAGATGCATGACAGTGATATATCAGCATATACTTACGAGCGCACTTTGATGATGGAA CAAAGGTCCCAGATGCTCCGGCACATGCGGCTATCCAAAACAGAGCGAGACAGAGAGGCACAATTGGTGA AAGACCGAAACTCAATGCTACGATTGACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAAACCTATCA GGAGAAGGTGCACATGACTTGGACAAAAGACAAGTACATGGCATCCCGGGGACAAAAAGCGAAGTCAATG GAAGGATTCCAGGACCTGCTTAACATGCGTCCGGACCAGTCCAATGTGAGGCGGATGCATACAGCAGTGA AACTCAACGAGGTTATAGTTAACAAGTCCCATGAAGCAAAGCTGGTTTTATTGAATATGCCAGGGCCACC CCGAAACCCTGAGGGTGATGAAAACTACATGGAGTTCCTAGAGGTGCTTACCGAGGGACTAGAGCGAGTC CTACTTGTCCGGGGTGGTGGCAGTGAAGTGATCACCATTTATTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214194 representing NM_001042497
Red=Cloning site Green=Tags(s) MHPPETTTKMASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSL ATVALDPPSDRTSHPQDVIEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMAN YTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVL ICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYI VPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKS SFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGI PGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNR SGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIG SFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPI LSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYK YIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKA GKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNT VVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVWWIVHDGGMLML LPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMME QRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKAKSM EGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERV LLVRGGGSEVITIYS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001042497 |
ORF Size | 3405 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001042497.2 |
RefSeq Size | 7457 bp |
RefSeq ORF | 3408 bp |
Locus ID | 9990 |
UniProt ID | Q9UHW9 |
Protein Families | Transmembrane |
Gene Summary | This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214194 | SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 6 |
CNY 11,310.00 |
|
RC214194L3 | Lenti-ORF clone of SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 6 |
CNY 13,210.00 |
|
RC214194L4 | Lenti-ORF clone of SLC12A6 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 6 |
CNY 13,210.00 |
|
SC311421 | SLC12A6 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 6 |
CNY 21,850.00 |
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