SLC12A6 (NM_001042494) Human Tagged ORF Clone
CAT#: RG214078
- TrueORF®
SLC12A6 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 3
ORF Plasmid: DDK
"NM_001042494" in other vectors (4)
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CNY 11,970.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ACCPN; KCC3; KCC3A; KCC3B |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214078 representing NM_001042494
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGATGTCTGGGGCCACCACTTCGCTGGCAACTGTTGCACTGGATCCACCCAGTGACCGGACTT CTCACCCCCAGGATGTCATCGAGGACCTGAGTCAGAACTCCATCACAGGGGAACACAGCCAACTGTTAGA CGACGGACATAAGAAAGCTCGAAATGCTTATCTCAATAATTCCAATTATGAAGAAGGAGATGAATATTTT GATAAAAATTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCGAAGGTGTCTTCCCTCCTCAACCGCA TGGCCAATTACACTAATCTGACTCAAGGAGCAAAGGAACATGAAGAGGCAGAAAACATCACTGAAGGGAA AAAGAAGCCCACCAAGACCCCCCAAATGGGTACCTTCATGGGTGTCTACCTCCCATGTCTACAAAATATT TTTGGAGTGATCCTTTTTTTACGCCTTACATGGGTGGTGGGCACAGCTGGAGTTCTTCAGGCTTTTGCAA TTGTCCTTATCTGCTGCTGCTGTACAATGTTGACTGCTATCTCCATGAGTGCCATTGCCACTAATGGAGT GGTGCCAGCTGGGGGCTCATACTTTATGATTTCCCGGGCACTGGGCCCAGAGTTTGGTGGGGCTGTTGGC CTCTGCTTTTATCTTGGTACCACATTTGCAGCAGCCATGTACATCCTTGGTGCCATTGAAATCTTTCTGG TCTATATCGTCCCCCGAGCTGCCATCTTTCACAGTGATGACGCACTCAAGGAATCAGCAGCCATGCTAAA TAACATGCGTGTCTACGGCACAGCTTTCTTGGTCCTTATGGTATTAGTGGTATTTATCGGCGTACGCTAT GTGAACAAGTTTGCCTCACTTTTCCTGGCCTGTGTCATTGTGTCCATCTTGGCCATCTATGCTGGAGCCA TCAAGTCTTCTTTTGCTCCTCCACACTTCCCGGTCTGCATGCTGGGTAACCGCACCCTTTCATCAAGACA CATTGACGTTTGCTCTAAGACCAAGGAAATTAACAACATGACAGTCCCATCAAAGTTATGGGGATTCTTC TGTAACTCGAGTCAATTTTTCAATGCCACCTGTGATGAATACTTTGTTCACAATAACGTCACTTCAATCC AGGGCATTCCTGGATTGGCTAGTGGTATAATTACAGAGAATCTTTGGAGTAATTACCTACCCAAGGGAGA GATCATCGAAAAGCCTTCAGCCAAATCTTCTGATGTCTTAGGCAGCTTAAACCATGAATATGTTCTTGTT GACATCACCACCTCCTTCACGCTTCTGGTGGGAATCTTCTTTCCCTCTGTTACAGGTATCATGGCTGGAT CAAACAGATCTGGAGATCTGAAAGATGCTCAGAAGTCTATTCCGATTGGTACTATCCTTGCCATCCTGAC CACCTCCTTTGTTTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATTGAAGGGGTTGTTCTCAGAGAC AAGTTCGGTGATGCTGTGAAAGGTAATTTGGTGGTAGGCACCTTATCTTGGCCATCCCCATGGGTGATTG TTATTGGCTCCTTCTTTTCAACATGTGGGGCTGGACTTCAGAGCCTCACAGGTGCACCGAGGCTGCTACA AGCTATTGCCAAGGATAACATCATACCGTTTCTGAGGGTTTTTGGCCACAGCAAAGCCAATGGGGAACCT ACCTGGGCTTTACTTCTAACTGCTGCCATTGCAGAGCTTGGAATACTCATTGCCTCCCTGGATCTTGTGG CCCCAATTCTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTAAACTTGGCATGTGCCTTGCAAACATT ACTTCGAACACCCAACTGGAGACCCCGATTCCGCTACTACCATTGGGCCCTTTCTTTCATGGGAATGAGT ATCTGTCTGGCTCTGATGTTCATTTCTTCCTGGTATTATGCCATTGTAGCCATGGTAATAGCTGGTATGA TCTACAAGTACATTGAATACCAAGGAGCTGAGAAAGAATGGGGTGATGGTATCCGTGGGCTGTCCCTCAG TGCAGCCCGGTTTGCTTTGCTTCGATTGGAGGAAGGACCTCCACACACTAAAAACTGGAGGCCTCAGTTG CTTGTATTACTGAAACTAGATGAAGACTTACATGTCAAGCATCCTCGCCTCCTCACCTTTGCCTCACAGC TCAAAGCAGGAAAAGGTCTCACTATTGTGGGCTCTGTCATCGTGGGGAACTTCCTAGAGAACTACGGTGA AGCTTTAGCTGCTGAGCAGACCATAAAGCACCTAATGGAGGCAGAGAAGGTAAAAGGATTCTGCCAGCTG GTGGTGGCCGCCAAGCTGAGAGAGGGCATTTCCCACCTCATCCAGTCATGTGGCCTTGGGGGCATGAAGC ACAACACGGTGGTGATGGGCTGGCCTAATGGCTGGCGTCAAAGCGAAGATGCCCGCGCTTGGAAGACTTT TATTGGCACAGTTCGAGTGACAACTGCTGCCCATCTTGCACTGCTGGTGGCTAAAAACATCTCCTTCTTT CCCAGCAATGTGGAGCAATTTTCTGAGGGCAACATTGATGTGTGGTGGATTGTGCATGATGGGGGGATGC TTATGCTACTACCATTCCTACTGAAACAGCACAAGGTGTGGCGAAAGTGCAGCATACGGATCTTCACAGT AGCCCAATTAGAAGACAACAGTATCCAAATGAAGAAGGACCTAGCCACCTTCCTATATCACTTACGCATT GAGGCGGAGGTAGAAGTGGTGGAGATGCATGACAGTGATATATCAGCATATACTTACGAGCGCACTTTGA TGATGGAACAAAGGTCCCAGATGCTCCGGCACATGCGGCTATCCAAAACAGAGCGAGACAGAGAGGCACA ATTGGTGAAAGACCGAAACTCAATGCTACGATTGACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAA ACCTATCAGGAGAAGGTGCACATGACTTGGACAAAAGACAAGTACATGGCATCCCGGGGACAAAAAGCGA AGTCAATGGAAGGATTCCAGGACCTGCTTAACATGCGTCCGGACCAGTCCAATGTGAGGCGGATGCATAC AGCAGTGAAACTCAACGAGGTTATAGTTAACAAGTCCCATGAAGCAAAGCTGGTTTTATTGAATATGCCA GGGCCACCCCGAAACCCTGAGGGTGATGAAAACTACATGGAGTTCCTAGAGGTGCTTACCGAGGGACTAG AGCGAGTCCTACTTGTCCGGGGTGGTGGCAGTGAAGTGATCACCATTTATTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214078 representing NM_001042494
Red=Cloning site Green=Tags(s) MSEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYF DKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNI FGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVG LCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRY VNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFF CNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGSLNHEYVLV DITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRD KFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEP TWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMS ICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQL LVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQL VVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFF PSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRI EAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETE TYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMP GPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001042494 |
ORF Size | 3273 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001042494.2 |
RefSeq Size | 7688 bp |
RefSeq ORF | 3276 bp |
Locus ID | 9990 |
UniProt ID | Q9UHW9 |
Protein Families | Transmembrane |
Gene Summary | This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214078 | SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 3 |
CNY 7,600.00 |
|
RC214078L3 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 3, Myc-DDK-tagged |
CNY 12,830.00 |
|
RC214078L4 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 3, mGFP tagged |
CNY 12,830.00 |
|
SC311418 | SLC12A6 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 3 |
CNY 7,608.00 |
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