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TBX1 (NM_005992) Human Tagged ORF Clone
CAT＃: RG214058
- TrueORF®
TBX1 (tGFP-tagged) - Human T-box 1 (TBX1), transcript variant B. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_005992" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAFS; CATCH22; CTHM; DGCR; DGS; DORV; TBX1C; TGA; VCF; VCFS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214058 ORF sequence, codon optimized.
Due to the complexity of NM_005992, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTTTAGCACCGTGACACGGGATATGGAGGCGTTTACTGCATCCAGTCTCTCTAGCCTTGGTGCCG CCGGGGGATTCCCTGGCGCAGCATCACCCGGGGCTGATCCTTATGGCCCTCGAGAACCTCCTCCCCCCCC TCCTCGGTACGATCCATGCGCTGCCGCTGCCCCAGGTGCACCAGGGCCTCCACCTCCCCCACATGCTTAT CCCTTCGCTCCTGCAGCCGGTGCCGCTACCAGTGCAGCTGCTGAGCCAGAGGGACCTGGAGCTAGTTGTG CCGCTGCCGCAAAAGCGCCCGTCAAGAAGAACGCCAAGGTTGCTGGCGTGTCAGTGCAGCTGGAAATGAA GGCCCTCTGGGACGAGTTCAATCAGCTCGGGACCGAGATGATCGTTACAAAAGCCGGTCGGAGAATGTTC CCCACCTTTCAGGTTAAACTTTTTGGGATGGATCCTATGGCAGACTACATGCTGCTGATGGATTTCGTTC CAGTCGACGACAAGAGGTACCGCTACGCCTTCCACTCTAGTTCTTGGCTGGTGGCTGGGAAGGCGGATCC AGCCACCCCGGGCCGGGTGCACTATCACCCTGACAGCCCGGCAAAAGGCGCGCAATGGATGAAGCAGATT GTTTCATTTGACAAACTCAAACTGACAAACAACCTGCTCGACGACAACGGCCATATTATTCTCAATTCAA TGCACCGATACCAGCCAAGGTTTCATGTCGTGTATGTCGACCCTCGAAAGGACTCCGAAAAATATGCCGA GGAGAACTTTAAGACATTTGTGTTTGAGGAGACACGGTTCACCGCAGTCACTGCCTATCAGAACCATCGG ATAACCCAATTGAAGATTGCATCAAATCCGTTCGCCAAAGGTTTTCGAGACTGCGACCCGGAGGATTGGC CGCGCAACCATAGGCCTGGAGCCCTGCCATTGATGAGCGCATTCGCCAGATCCAGAAACCCCGTGGCCAG CCCCACTCAGCCGTCAGGCACAGAGAAGGGGCTCGTGACAGAGGGAAGCGGCCTGCAGCCGGGACTGCTT GACGTCCTGCTTAAACCCCCATCCAAGAAAAGTGAGAGCCTGCGACCTCCTCATTGCAAAGACACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214058 representing NM_005992
Red=Cloning site Green=Tags(s) MHFSTVTRDMEAFTASSLSSLGAAGGFPGAASPGADPYGPREPPPPPPRYDPCAAAAPGAPGPPPPPHAY PFAPAAGAATSAAAEPEGPGASCAAAAKAPVKKNAKVAGVSVQLEMKALWDEFNQLGTEMIVTKAGRRMF PTFQVKLFGMDPMADYMLLMDFVPVDDKRYRYAFHSSSWLVAGKADPATPGRVHYHPDSPAKGAQWMKQI VSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVYVDPRKDSEKYAEENFKTFVFEETRFTAVTAYQNHR ITQLKIASNPFAKGFRDCDPEDWPRNHRPGALPLMSAFARSRNPVASPTQPSGTEKGLVTEGSGLQPGLL DVLLKPPSKKSESLRPPHCKDT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005992 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005992.1, NP_005983.1 |
| RefSeq Size | 1538 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 6899 |
| UniProt ID | O43435 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214058 | TBX1 (Myc-DDK-tagged)-Human T-box 1 (TBX1), transcript variant B. Note: ORF is codon optimized |
CNY 3,656.00 |
|
| RC214058L3 | Lenti-ORF clone of TBX1 (Myc-DDK-tagged)-Human T-box 1 (TBX1), transcript variant B. Note: ORF is codon optimized |
CNY 5,890.00 |
|
| RC214058L4 | Lenti-ORF clone of TBX1 (mGFP-tagged)-Human T-box 1 (TBX1), transcript variant B. Note: ORF is codon optimized |
CNY 5,890.00 |
|
| SC303716 | TBX1 (untagged)-Human T-box 1 (TBX1), transcript variant B |
CNY 6,080.00 |
