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SLC12A6 (NM_133647) Human Tagged ORF Clone
CAT＃: RG214022
- TrueORF®
SLC12A6 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 1
ORF Plasmid: DDK
"NM_133647" in other vectors (4)
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CNY 12,640.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACCPN; KCC3; KCC3A; KCC3B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214022 representing NM_133647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCCTCCAGAAACCACCACCAAGATGGCTTCAGTTCGGTTCATGGTGACACCGACAAAGATCGATG ACATTCCAGGTTTGTCAGACACCAGTCCGGACCTCAGCTCTCGATCTAGTTCCCGAGTAAGATTTAGCTC CCGGGAAAGCGTGCCTGAAACAAGCCGGAGTGAGCCTATGAGTGAGATGTCTGGGGCCACCACTTCGCTG GCAACTGTTGCACTGGATCCACCCAGTGACCGGACTTCTCACCCCCAGGATGTCATCGAGGACCTGAGTC AGAACTCCATCACAGGGGAACACAGCCAACTGTTAGACGACGGACATAAGAAAGCTCGAAATGCTTATCT CAATAATTCCAATTATGAAGAAGGAGATGAATATTTTGATAAAAATTTGGCACTCTTTGAGGAAGAAATG GACACCAGACCGAAGGTGTCTTCCCTCCTCAACCGCATGGCCAATTACACTAATCTGACTCAAGGAGCAA AGGAACATGAAGAGGCAGAAAACATCACTGAAGGGAAAAAGAAGCCCACCAAGACCCCCCAAATGGGTAC CTTCATGGGTGTCTACCTCCCATGTCTACAAAATATTTTTGGAGTGATCCTTTTTTTACGCCTTACATGG GTGGTGGGCACAGCTGGAGTTCTTCAGGCTTTTGCAATTGTCCTTATCTGCTGCTGCTGTACAATGTTGA CTGCTATCTCCATGAGTGCCATTGCCACTAATGGAGTGGTGCCAGCTGGGGGCTCATACTTTATGATTTC CCGGGCACTGGGCCCAGAGTTTGGTGGGGCTGTTGGCCTCTGCTTTTATCTTGGTACCACATTTGCAGCA GCCATGTACATCCTTGGTGCCATTGAAATCTTTCTGGTCTATATCGTCCCCCGAGCTGCCATCTTTCACA GTGATGACGCACTCAAGGAATCAGCAGCCATGCTAAATAACATGCGTGTCTACGGCACAGCTTTCTTGGT CCTTATGGTATTAGTGGTATTTATCGGCGTACGCTATGTGAACAAGTTTGCCTCACTTTTCCTGGCCTGT GTCATTGTGTCCATCTTGGCCATCTATGCTGGAGCCATCAAGTCTTCTTTTGCTCCTCCACACTTCCCGG TCTGCATGCTGGGTAACCGCACCCTTTCATCAAGACACATTGACGTTTGCTCTAAGACCAAGGAAATTAA CAACATGACAGTCCCATCAAAGTTATGGGGATTCTTCTGTAACTCGAGTCAATTTTTCAATGCCACCTGT GATGAATACTTTGTTCACAATAACGTCACTTCAATCCAGGGCATTCCTGGATTGGCTAGTGGTATAATTA CAGAGAATCTTTGGAGTAATTACCTACCCAAGGGAGAGATCATCGAAAAGCCTTCAGCCAAATCTTCTGA TGTCTTAGGCAGCTTAAACCATGAATATGTTCTTGTTGACATCACCACCTCCTTCACGCTTCTGGTGGGA ATCTTCTTTCCCTCTGTTACAGGTATCATGGCTGGATCAAACAGATCTGGAGATCTGAAAGATGCTCAGA AGTCTATTCCGATTGGTACTATCCTTGCCATCCTGACCACCTCCTTTGTTTATTTAAGCAATGTTGTCCT TTTTGGTGCATGTATTGAAGGGGTTGTTCTCAGAGACAAGTTCGGTGATGCTGTGAAAGGTAATTTGGTG GTAGGCACCTTATCTTGGCCATCCCCATGGGTGATTGTTATTGGCTCCTTCTTTTCAACATGTGGGGCTG GACTTCAGAGCCTCACAGGTGCACCGAGGCTGCTACAAGCTATTGCCAAGGATAACATCATACCGTTTCT GAGGGTTTTTGGCCACAGCAAAGCCAATGGGGAACCTACCTGGGCTTTACTTCTAACTGCTGCCATTGCA GAGCTTGGAATACTCATTGCCTCCCTGGATCTTGTGGCCCCAATTCTTTCCATGTTTTTTCTCATGTGTT ACCTCTTTGTAAACTTGGCATGTGCCTTGCAAACATTACTTCGAACACCCAACTGGAGACCCCGATTCCG CTACTACCATTGGGCCCTTTCTTTCATGGGAATGAGTATCTGTCTGGCTCTGATGTTCATTTCTTCCTGG TATTATGCCATTGTAGCCATGGTAATAGCTGGTATGATCTACAAGTACATTGAATACCAAGGAGCTGAGA AAGAATGGGGTGATGGTATCCGTGGGCTGTCCCTCAGTGCAGCCCGGTTTGCTTTGCTTCGATTGGAGGA AGGACCTCCACACACTAAAAACTGGAGGCCTCAGTTGCTTGTATTACTGAAACTAGATGAAGACTTACAT GTCAAGCATCCTCGCCTCCTCACCTTTGCCTCACAGCTCAAAGCAGGAAAAGGTCTCACTATTGTGGGCT CTGTCATCGTGGGGAACTTCCTAGAGAACTACGGTGAAGCTTTAGCTGCTGAGCAGACCATAAAGCACCT AATGGAGGCAGAGAAGGTAAAAGGATTCTGCCAGCTGGTGGTGGCCGCCAAGCTGAGAGAGGGCATTTCC CACCTCATCCAGTCATGTGGCCTTGGGGGCATGAAGCACAACACGGTGGTGATGGGCTGGCCTAATGGCT GGCGTCAAAGCGAAGATGCCCGCGCTTGGAAGACTTTTATTGGCACAGTTCGAGTGACAACTGCTGCCCA TCTTGCACTGCTGGTGGCTAAAAACATCTCCTTCTTTCCCAGCAATGTGGAGCAATTTTCTGAGGGCAAC ATTGATGTGTGGTGGATTGTGCATGATGGGGGGATGCTTATGCTACTACCATTCCTACTGAAACAGCACA AGGTGTGGCGAAAGTGCAGCATACGGATCTTCACAGTAGCCCAATTAGAAGACAACAGTATCCAAATGAA GAAGGACCTAGCCACCTTCCTATATCACTTACGCATTGAGGCGGAGGTAGAAGTGGTGGAGATGCATGAC AGTGATATATCAGCATATACTTACGAGCGCACTTTGATGATGGAACAAAGGTCCCAGATGCTCCGGCACA TGCGGCTATCCAAAACAGAGCGAGACAGAGAGGCACAATTGGTGAAAGACCGAAACTCAATGCTACGATT GACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAAACCTATCAGGAGAAGGTGCACATGACTTGGACA AAAGACAAGTACATGGCATCCCGGGGACAAAAAGCGAAGTCAATGGAAGGATTCCAGGACCTGCTTAACA TGCGTCCGGACCAGTCCAATGTGAGGCGGATGCATACAGCAGTGAAACTCAACGAGGTTATAGTTAACAA GTCCCATGAAGCAAAGCTGGTTTTATTGAATATGCCAGGGCCACCCCGAAACCCTGAGGGTGATGAAAAC TACATGGAGTTCCTAGAGGTGCTTACCGAGGGACTAGAGCGAGTCCTACTTGTCCGGGGTGGTGGCAGTG AAGTGATCACCATTTATTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214022 representing NM_133647
Red=Cloning site Green=Tags(s) MHPPETTTKMASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSL ATVALDPPSDRTSHPQDVIEDLSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEM DTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTW VVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAA AMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLAC VIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFFCNSSQFFNATC DEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVG IFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLV VGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIA ELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSW YYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLH VKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGIS HLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGN IDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHD SDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWT KDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDEN YMEFLEVLTEGLERVLLVRGGGSEVITIYS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133647 |
| ORF Size | 3450 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133647.1, NP_598408.1 |
| RefSeq Size | 7502 bp |
| RefSeq ORF | 3453 bp |
| Locus ID | 9990 |
| UniProt ID | Q9UHW9 |
| Protein Families | Transmembrane |
| Gene Summary | This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214022 | SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 1 |
CNY 11,500.00 |
|
| RC214022L3 | Lenti-ORF clone of SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 1 |
CNY 13,400.00 |
|
| RC214022L4 | Lenti-ORF clone of SLC12A6 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 1 |
CNY 13,400.00 |
|
| SC311424 | SLC12A6 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 1 |
CNY 12,824.00 |
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