United States
Germany
Japan
United Kingdom
China
GRAF (ARHGAP26) (NM_015071) Human Tagged ORF Clone
CAT＃: RG213996
- TrueORF®
ARHGAP26 (tGFP-tagged) - Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 1
ORF Plasmid: DDK
"NM_015071" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,560.00
CNY 9,500.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GRAF; GRAF1; OPHN1L; OPHN1L1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213996 representing NM_015071
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTCCCAGCGCTCGAGTTCAGCGACTGCTGCCTCGATAGTCCGCACTTCCGAGAGACGCTCAAGT CGCACGAAGCAGAGCTGGACAAGACCAACAAATTCATCAAGGAGCTCATCAAGGACGGGAAGTCACTCAT AAGCGCGCTCAAGAATTTGTCTTCAGCGAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAG TGCATAGGAGATGCAGAAACAGATGATGAGATGTGTATAGCAAGATCTTTGCAGGAGTTTGCCACTGTCC TCAGGAATCTTGAAGATGAACGGATACGGATGATTGAGAATGCCAGCGAGGTGCTCATCACTCCCTTGGA GAAGTTTCGAAAGGAACAGATCGGGGCTGCCAAGGAAGCCAAAAAGAAGTATGACAAAGAGACAGAAAAG TATTGTGGCATCTTAGAAAAACACTTGAATTTGTCTTCCAAAAAGAAAGAATCTCAGCTTCAGGAGGCAG ACAGCCAAGTGGACCTGGTCCGGCAGCATTTCTATGAAGTATCCCTGGAATATGTCTTCAAGGTGCAGGA AGTCCAAGAGAGAAAGATGTTTGAGTTTGTGGAGCCTCTGCTGGCCTTCCTGCAAGGACTCTTCACTTTC TATCACCATGGTTACGAACTGGCCAAGGATTTCGGGGACTTCAAGACACAGTTAACCATTAGCATACAGA ACACAAGAAATCGCTTTGAAGGCACTAGATCAGAAGTGGAATCACTGATGAAAAAGATGAAGGAGAATCC CCTTGAGCACAAGACCATCAGTCCCTACACCATGGAGGGATACCTCTACGTGCAGGAGAAACGTCACTTT GGAACTTCTTGGGTGAAGCACTACTGTACATATCAACGGGATTCCAAACAAATCACCATGGTACCATTTG ACCAAAAGTCAGGAGGAAAAGGGGGAGAAGATGAATCAGTTATCCTCAAATCCTGCACACGGCGGAAAAC AGACTCCATTGAGAAGAGGTTTTGCTTTGATGTGGAAGCAGTAGACAGGCCAGGGGTTATCACCATGCAA GCTTTGTCGGAAGAGGACCGGAGGCTCTGGATGGAAGCCATGGATGGCCGGGAACCTGTCTACAACTCGA ACAAAGACAGCCAGAGTGAAGGGACTGCGCAGTTGGACAGCATTGGCTTCAGCATAATCAGGAAATGCAT CCATGCTGTGGAAACCAGAGGGATCAACGAGCAAGGGCTGTATCGAATTGTGGGTGTCAACTCCAGAGTG CAGAAGTTGCTGAGTGTCCTGATGGACCCCAAGACTGCTTCTGAGACAGAAACAGATATCTGTGCTGAAT GGGAGATAAAGACCATCACTAGTGCTCTGAAGACCTACCTAAGAATGCTTCCAGGACCACTCATGATGTA CCAGTTTCAAAGAAGTTTCATCAAAGCAGCAAAACTGGAGAACCAGGAGTCTCGGGTCTCTGAAATCCAC AGCCTTGTTCATCGGCTCCCAGAGAAAAATCGGCAGATGTTACAGCTGCTCATGAACCACTTGGCAAATG TTGCTAACAACCACAAGCAGAATTTGATGACGGTGGCAAACCTTGGTGTGGTGTTTGGACCCACTCTGCT GAGGCCTCAGGAAGAAACAGTAGCAGCCATCATGGACATCAAATTTCAGAACATTGTCATTGAGATCCTA ATAGAAAACCACGAAAAGATATTTAACACCGTGCCCGATATGCCTCTCACCAATGCCCAGCTGCACCTGT CTCGGAAGAAGAGCAGTGACTCCAAGCCCCCGTCCTGCAGCGAGAGGCCCCTGACGCTCTTCCACACCGT TCAGTCAACAGAGAAACAGGAACAAAGGAACAGCATCATCAACTCCAGTTTGGAATCTGTCTCATCAAAT CCAAACAGCATCCTTAATTCCAGCAGCAGCTTACAGCCCAACATGAACTCCAGTGACCCAGACCTGGCTG TGGTCAAACCCACCCGGCCCAACTCACTCCCCCCGAATCCAAGCCCAACTTCACCCCTCTCGCCATCTTG GCCCATGTTCTCGGCGCCATCCAGCCCTATGCCCACCTCATCCACGTCCAGCGACTCATCCCCCGTCAGG TCTGTTGCAGGGTTTGTTTGGTTTTCTGTTGCTGCCGTTGTTCTCTCATTGGCTCGGTCCTCTCTTCATG CAGTGTTCAGCCTCCTCGTCAACTTTGTTCCCTGCCATCCAAACCTGCACTTGCTTTTTGACAGGCCAGA AGAAGCGGTACATGAAGACTCCAGCACACCGTTCCGGAAGGCAAAAGCCTTGTATGCCTGCAAAGCTGAA CATGACTCAGAACTTTCGTTCACAGCAGGCACGGTCTTCGATAATGTTCACCCATCTCAGGAGCCTGGCT GGTTGGAGGGGACTCTGAACGGAAAGACTGGCCTCATCCCTGAGAATTACGTGGAGTTCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213996 representing NM_015071
Red=Cloning site Green=Tags(s) MGLPALEFSDCCLDSPHFRETLKSHEAELDKTNKFIKELIKDGKSLISALKNLSSAKRKFADSLNEFKFQ CIGDAETDDEMCIARSLQEFATVLRNLEDERIRMIENASEVLITPLEKFRKEQIGAAKEAKKKYDKETEK YCGILEKHLNLSSKKKESQLQEADSQVDLVRQHFYEVSLEYVFKVQEVQERKMFEFVEPLLAFLQGLFTF YHHGYELAKDFGDFKTQLTISIQNTRNRFEGTRSEVESLMKKMKENPLEHKTISPYTMEGYLYVQEKRHF GTSWVKHYCTYQRDSKQITMVPFDQKSGGKGGEDESVILKSCTRRKTDSIEKRFCFDVEAVDRPGVITMQ ALSEEDRRLWMEAMDGREPVYNSNKDSQSEGTAQLDSIGFSIIRKCIHAVETRGINEQGLYRIVGVNSRV QKLLSVLMDPKTASETETDICAEWEIKTITSALKTYLRMLPGPLMMYQFQRSFIKAAKLENQESRVSEIH SLVHRLPEKNRQMLQLLMNHLANVANNHKQNLMTVANLGVVFGPTLLRPQEETVAAIMDIKFQNIVIEIL IENHEKIFNTVPDMPLTNAQLHLSRKKSSDSKPPSCSERPLTLFHTVQSTEKQEQRNSIINSSLESVSSN PNSILNSSSSLQPNMNSSDPDLAVVKPTRPNSLPPNPSPTSPLSPSWPMFSAPSSPMPTSSTSSDSSPVR SVAGFVWFSVAAVVLSLARSSLHAVFSLLVNFVPCHPNLHLLFDRPEEAVHEDSSTPFRKAKALYACKAE HDSELSFTAGTVFDNVHPSQEPGWLEGTLNGKTGLIPENYVEFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015071 |
| ORF Size | 2442 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015071.3 |
| RefSeq Size | 6518 bp |
| RefSeq ORF | 2445 bp |
| Locus ID | 23092 |
| UniProt ID | Q9UNA1 |
| Domains | RhoGAP, SH3, PH |
| Protein Families | Druggable Genome |
| Gene Summary | Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213996 | ARHGAP26 (Myc-DDK-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 1 |
CNY 5,960.00 |
|
| RC213996L1 | Lenti ORF clone of Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 1, Myc-DDK-tagged |
CNY 8,360.00 |
|
| RC213996L2 | Lenti ORF clone of Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RC213996L3 | Lenti ORF clone of Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 1, Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC213996L4 | Lenti ORF clone of Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| SC114705 | ARHGAP26 (untagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 1 |
CNY 5,968.00 |
