LCTL (NM_207338) Human Tagged ORF Clone
CAT#: RG213528
- TrueORF®
LCTL (tGFP-tagged) - Human lactase-like (LCTL)
ORF Plasmid: DDK
"NM_207338" in other vectors (6)
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CNY 7,928.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | KLG; KLPH |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG213528 representing NM_207338
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCAGTGTGGGTCGCCACCCTTCTGTGGATGCTACTGCTGGTGCCCAGGCTGGGGGCCGCCCGGA AGGGGTCCCCAGAAGAGGCCTCCTTCTACTATGGAACCTTCCCTCTTGGCTTCTCCTGGGGCGTGGGCAG TTCTGCCTACCAGACGGAGGGCGCCTGGGACCAGGACGGGAAAGGGCCTAGCATCTGGGACGTCTTCACA CACAGTGGGAAGGGGAAAGTGCTTGGGAATGAGACGGCAGATGTAGCCTGTGACGGCTACTACAAGGTCC AGGAGGACATCATTCTGCTGAGGGAACTGCACGTCAACCACTACCGATTCTCCCTGTCTTGGCCCCGGCT CCTGCCCACAGGCATCCGAGCCGAGCAGGTGAACAAGAAGGGAATCGAATTCTACAGTGATCTTATCGAT GCCCTTCTGAGCAGCAACATCACTCCCATCGTGACCTTGCACCACTGGGATCTGCCACAGCTGCTCCAGG TCAAATACGGTGGGTGGCAGAATGTGAGCATGGCCAACTACTTCAGAGACTACGCCAACCTGTGCTTTGA GGCCTTTGGGGACCGTGTGAAGCACTGGATCACGTTCAGTGATCCTCGGGCAATGGCAGAAAAAGGCTAT GAGACGGGCCACCATGCGCCGGGCCTGAAGCTCCGCGGCACCGGCCTGTACAAGGCAGCACACCACATCA TTAAGGCCCACGCCAAAGCCTGGCATTCTTATAACACCACGTGGCGCAGCAAGCAGCAAGGTCTGGTGGG AATTTCATTGAACTGTGACTGGGGGGAACCTGTGGACATTAGTAACCCCAAGGACCTAGAGGCTGCCGAG AGATACCTACAGTTCTGTCTGGGCTGGTTTGCCAACCCCATTTATGCCGGTGACTACCCCCAAGTCATGA AGGACTACATTGGAAGAAAGAGTGCAGAGCAAGGCCTGGAGATGTCGAGGTTACCGGTGTTCTCACTCCA GGAGAAGAGCTACATTAAAGGCACATCCGATTTCTTGGGATTAGGTCATTTTACTACTCGGTACATCACG GAAAGGAACTACCCCTCCCGCCAGGGGCCCAGCTACCAGAACGATCGTGACTTGATAGAGCTGGTTGACC CAAACTGGCCAGATCTGGGGTCTAAATGGCTATATTCTGTGCCATGGGGATTTAGGAGGCTCCTTAACTT TGCTCAGACTCAATACGGTGATCCTCCCATATATGTGATGGAAAATGGAGCATCTCAAAAATTCCACTGT ACTCAATTATGTGATGAGTGGAGAATTCAATACCTTAAAGGATACATAAATGAAATGCTAAAAGCTATAA AAGATGGTGCTAATATAAAGGGGTATACTTCCTGGTCTCTGTTGGATAAGTTTGAATGGGAGAAAGGATA CTCAGATAGATATGGATTCTACTATGTTGAATTTAACGACAGAAATAAGCCTCGCTATCCAAAGGCTTCA GTTCAATATTACAAGAAGATTATCATTGCCAATGGGTTTCCCAATCCAAGAGAGGTGGAAAGTTGGTACC TCAAAGCTTTGGAAACTTGCTCTATCAACAATCAGATGCTTGCTGCAGAGCCCTTGCTAAGTCACATGCA AATGGTTACGGAGATCGTGGTACCCACTGTCTGCTCCCTCTGTGTCCTCATCACTGCTGTTCTACTAATG CTCCTCCTGAGGAGGCAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213528 representing NM_207338
Red=Cloning site Green=Tags(s) MKPVWVATLLWMLLLVPRLGAARKGSPEEASFYYGTFPLGFSWGVGSSAYQTEGAWDQDGKGPSIWDVFT HSGKGKVLGNETADVACDGYYKVQEDIILLRELHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLID ALLSSNITPIVTLHHWDLPQLLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMAEKGY ETGHHAPGLKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGEPVDISNPKDLEAAE RYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKGTSDFLGLGHFTTRYIT ERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHC TQLCDEWRIQYLKGYINEMLKAIKDGANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKAS VQYYKKIIIANGFPNPREVESWYLKALETCSINNQMLAAEPLLSHMQMVTEIVVPTVCSLCVLITAVLLM LLLRRQS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_207338 |
ORF Size | 1701 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_207338.4 |
RefSeq Size | 2128 bp |
RefSeq ORF | 1704 bp |
Locus ID | 197021 |
UniProt ID | Q6UWM7 |
Protein Families | Transmembrane |
Gene Summary | This gene encodes a member of family 1 glycosidases. Glycosidases are enzymes that hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. Most members of family 1 have two conserved glutamic acid residues, which are required for enzymatic activity. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail. It lacks one of the conserved glutamic acid residues important for catalysis, and its function remains to be determined (PMID: 12084582). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213528 | LCTL (Myc-DDK-tagged)-Human lactase-like (LCTL) |
CNY 6,328.00 |
|
RC213528L1 | Lenti ORF clone of Human lactase-like (LCTL), Myc-DDK-tagged |
CNY 8,728.00 |
|
RC213528L2 | Lenti ORF clone of Human lactase-like (LCTL), mGFP tagged |
CNY 6,460.00 |
|
RC213528L3 | Lenti ORF clone of Human lactase-like (LCTL), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC213528L4 | Lenti ORF clone of Human lactase-like (LCTL), mGFP tagged |
CNY 6,460.00 |
|
SC308461 | LCTL (untagged)-Human lactase-like (LCTL) |
CNY 6,344.00 |