United States
Germany
Japan
United Kingdom
China
Entactin (NID1) (NM_002508) Human Tagged ORF Clone
CAT＃: RG213422
- TrueORF®
NID1 (tGFP-tagged) - Human nidogen 1 (NID1)
ORF Plasmid: DDK
"NM_002508" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 10,280.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NID |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213422 representing NM_002508
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGCCTCGAGCAGCCGGATCCGGGCTGCGTGGACGCGGGCGCTGCTGCTGCCGCTGCTGCTGGCGG GGCCTGTGGGCTGCCTGAGCCGCCAGGAGCTCTTTCCCTTCGGCCCCGGACAGGGGGACCTGGAGCTGGA GGACGGGGATGACTTCGTCTCTCCTGCCCTGGAGCTGAGTGGGGCGCTCCGCTTCTACGACAGATCCGAC ATCGACGCAGTCTACGTCACCACAAATGGCATCATTGCTACGAGTGAACCCCCGGCCAAAGAATCCCATC CCGGGCTCTTCCCACCAACATTCGGTGCAGTCGCCCCTTTCCTGGCGGACTTGGACACGACCGATGGCCT GGGGAAGGTTTATTATCGAGAAGACTTATCCCCCTCCATCACTCAGCGAGCAGCAGAGTGTGTCCACAGA GGGTTCCCGGAGATCTCTTTCCAGCCTAGTAGCGCGGTGGTTGTCACTTGGGAATCCGTGGCCCCCTACC AAGGGCCCAGCAGGGACCCAGACCAGAAAGGCAAGAGAAACACGTTCCAGGCTGTTCTAGCCTCCTCTGA TTCCAGCTCCTATGCCATTTTCCTTTATCCTGAGGATGGTCTGCAGTTCCATACGACATTCTCAAAGAAG GAAAACAACCAAGTTCCTGCCGTGGTTGCATTCAGTCAAGGTTCAGTGGGATTCTTATGGAAGAGCAACG GAGCTTATAACATATTTGCTAATGACAGGGAATCAATTGAAAATTTGGCCAAGAGTAGTAACTCTGGGCA GCAGGGTGTCTGGGTGTTTGAGATTGGGAGTCCAGCCACCACCAATGGCGTGGTGCCTGCAGACGTGATC CTCGGAACTGAAGATGGGGCAGAGTATGATGATGAGGATGAAGATTATGACCTGGCGACCACTCGTCTGG GCCTGGAGGATGTGGGCACCACGCCCTTCTCCTACAAGGCTCTGAGAAGGGGAGGTGCTGACACATACAG TGTGCCCAGCGTCCTCTCCCCGCGCCGGGCAGCTACCGAAAGGCCCCTTGGACCTCCCACAGAGAGAACC AGGTCTTTCCAGTTGGCAGTGGAGACTTTTCACCAGCAGCACCCTCAGGTCATAGATGTGGATGAAGTTG AGGAAACAGGAGTTGTTTTCAGCTATAACACGGATTCCCGCCAGACGTGTGCTAACAACAGACACCAGTG CTCGGTGCACGCAGAGTGCAGGGACTACGCCACGGGCTTCTGCTGCAGCTGTGTCGCTGGCTATACGGGC AATGGCAGGCAATGTGTTGCAGAAGGTTCCCCCCAGCGAGTCAATGGCAAGGTGAAAGGAAGGATCTTTG TGGGGAGCAGCCAGGTCCCCATTGTCTTTGAGAACACTGACCTCCACTCTTACGTAGTAATGAACCACGG GCGCTCCTACACAGCCATCAGCACCATTCCCGAGACCGTTGGATATTCTCTGCTTCCACTGGCCCCAGTT GGAGGCATCATTGGATGGATGTTTGCAGTGGAGCAGGACGGATTCAAGAATGGGTTCAGCATCACCGGGG GTGAGTTCACTCGCCAGGCTGAGGTGACCTTCGTGGGGCACCCGGGCAATCTGGTCATTAAGCAGCGGTT CAGCGGCATCGATGAGCATGGGCACCTGACCATCGACACGGAGCTGGAGGGCCGCGTGCCGCAGATTCCG TTCGGCTCCTCCGTGCACATTGAGCCCTACACGGAGCTGTACCACTACTCCACCTCAGTGATCACTTCCT CCTCCACCCGGGAGTACACGGTGACTGAGCCCGAGCGAGATGGGGCATCTCCTTCACGCATCTACACTTA CCAGTGGCGCCAGACCATCACCTTCCAGGAATGCGTCCACGATGACTCCCGGCCAGCCCTGCCCAGCACC CAGCAGCTCTCGGTGGACAGCGTGTTCGTCCTGTACAACCAGGAGGAGAAGATCTTGCGCTACGCTTTCA GCAACTCCATTGGGCCTGTGAGGGAAGGCTCCCCTGATGCTCTTCAGAATCCCTGCTACATCGGCACTCA TGGGTGTGACACCAACGCGGCCTGTCGCCCTGGTCCCAGGACACAGTTCACCTGCGAGTGCTCCATCGGC TTCCGAGGAGACGGGCGAACCTGCTATGATATTGATGAATGTTCAGAACAACCCTCAGTGTGTGGGAGCC ACACAATCTGCAATAATCACCCAGGAACCTTCCGCTGCGAGTGTGTGGAGGGCTACCAGTTTTCAGATGA GGGAACGTGTGTGGCTGTCGTGGACCAGCGCCCCATCAACTACTGTGAAACTGGCCTTCATAACTGCGAC ATACCCCAGCGGGCCCAGTGTATCTACACAGGAGGCTCCTCCTACACCTGTTCCTGCTTGCCAGGCTTTT CTGGGGATGGCCAAGCCTGCCAAGATGTAGATGAATGCCAGCCAAGCCGATGTCACCCTGACGCCTTCTG CTACAACACTCCAGGCTCTTTCACGTGCCAGTGCAAACCTGGTTATCAGGGAGACGGCTTCCGTTGCGTG CCCGGAGAGGTGGAGAAAACCCGGTGCCAGCACGAGCGAGAACACATTCTCGGGGCAGCGGGGGCGACAG ACCCACAGCGACCCATTCCTCCGGGGCTGTTCGTTCCTGAGTGCGATGCGCACGGGCACTACGCGCCCAC CCAGTGCCACGGCAGCACCGGCTACTGCTGGTGCGTGGATCGCGACGGCCGCGAGGTGGAGGGCACCAGG ACCAGGCCCGGGATGACGCCCCCGTGTCTGAGTACAGTGGCTCCCCCGATTCACCAAGGACCTGCGGTGC CTACCGCCGTGATCCCCTTGCCTCCTGGGACCCATTTACTCTTTGCCCAGACTGGGAAGATTGAGCGCCT GCCCCTGGAGGGAAATACCATGAGGAAGACAGAAGCAAAGGCGTTCCTTCATGTCCCGGCTAAAGTCATC ATTGGACTGGCCTTTGACTGCGTGGACAAGATGGTTTACTGGACGGACATCACTGAGCCTTCCATTGGGA GAGCTAGTCTACATGGTGGAGAGCCAACCACCATCATTAGACAAGATCTTGGAAGTCCAGAAGGTATCGC TGTTGATCACCTTGGCCGCAACATCTTCTGGACAGACTCTAACCTGGATCGAATAGAAGTGGCGAAGCTG GACGGCACGCAGCGCCGGGTGCTCTTTGAGACTGACCTGGTGAATCCCAGAGGCATTGTAACGGATTCCG TGAGAGGGAACCTTTACTGGACAGACTGGAACAGAGATAACCCCAAGATTGAAACTTCCTACATGGACGG CACGAACCGGAGGATCCTTGTGCAGGATGACCTGGGCTTGCCCAATGGACTGCACTTCGATGCGTTCTCA TCTCAGCTCTGCTGGGTGGATGCAGGCACCAATCGGGCGGAATGCCTGAACCCCAGTCAGCCCAGCAGAC GCAAGGCTCTCGAAGGGCTCCAGTATCCTTTTGCTGTGACGAGCTACGGGAAGAATCTGTATTTCACAGA CTGGAAGATGAATTCCGTGGTTGCTCTCGATCTTGCAATTTCCAAGGAGACGGATGCTTTCCAACCCCAC AAGCAGACCCGGCTGTATGGCATCACCACGGCCCTGTCTCAGTGTCCGCAAGGCCATAACTACTGCTCAG TGAACAATGGCGGCTGCACCCACCTATGCTTGGCCACCCCAGGGAGCAGGACCTGCCGTTGCCCTGACAA CACCTTGGGAGTTGACTGTATCGAACGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213422 representing NM_002508
Red=Cloning site Green=Tags(s) MLASSSRIRAAWTRALLLPLLLAGPVGCLSRQELFPFGPGQGDLELEDGDDFVSPALELSGALRFYDRSD IDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHR GFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKK ENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESIENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVI LGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERT RSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVHAECRDYATGFCCSCVAGYTG NGRQCVAEGSPQRVNGKVKGRIFVGSSQVPIVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPV GGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFVGHPGNLVIKQRFSGIDEHGHLTIDTELEGRVPQIP FGSSVHIEPYTELYHYSTSVITSSSTREYTVTEPERDGASPSRIYTYQWRQTITFQECVHDDSRPALPST QQLSVDSVFVLYNQEEKILRYAFSNSIGPVREGSPDALQNPCYIGTHGCDTNAACRPGPRTQFTCECSIG FRGDGRTCYDIDECSEQPSVCGSHTICNNHPGTFRCECVEGYQFSDEGTCVAVVDQRPINYCETGLHNCD IPQRAQCIYTGGSSYTCSCLPGFSGDGQACQDVDECQPSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCV PGEVEKTRCQHEREHILGAAGATDPQRPIPPGLFVPECDAHGHYAPTQCHGSTGYCWCVDRDGREVEGTR TRPGMTPPCLSTVAPPIHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAFLHVPAKVI IGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDHLGRNIFWTDSNLDRIEVAKL DGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNRDNPKIETSYMDGTNRRILVQDDLGLPNGLHFDAFS SQLCWVDAGTNRAECLNPSQPSRRKALEGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPH KQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIERK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002508 |
| ORF Size | 3741 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002508.1, NP_002499.1 |
| RefSeq Size | 4898 bp |
| RefSeq ORF | 3744 bp |
| Locus ID | 4811 |
| UniProt ID | P14543 |
| Domains | ldl_recept_b, thyroglobulin_1, EGF_CA, NIDO, EGF, EGF, G2F |
| Protein Families | Druggable Genome, Secreted Protein |
| Gene Summary | This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213422 | NID1 (Myc-DDK-tagged)-Human nidogen 1 (NID1) |
CNY 8,680.00 |
|
| RC213422L1 | Lenti ORF clone of Human nidogen 1 (NID1), Myc-DDK-tagged |
CNY 14,350.00 |
|
| RC213422L2 | Lenti ORF clone of Human nidogen 1 (NID1), mGFP tagged |
CNY 14,350.00 |
|
| RC213422L3 | Lenti ORF clone of Human nidogen 1 (NID1), Myc-DDK-tagged |
CNY 14,350.00 |
|
| RC213422L4 | Lenti ORF clone of Human nidogen 1 (NID1), mGFP tagged |
CNY 14,350.00 |
|
| SC118601 | NID1 (untagged)-Human nidogen 1 (NID1) |
CNY 8,688.00 |
其它Entactin产品
