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C20orf74 (RALGAPA2) (NM_020343) Human Tagged ORF Clone
CAT＃: RG213138
- TrueORF®
RALGAPA2 (tGFP-tagged) - Human Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2)
ORF Plasmid: DDK
"NM_020343" in other vectors (3)
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CNY 20,900.00
货期*
8周
规格
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经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AS250; bA287B20.1; C20orf74; dJ1049G11; dJ1049G11.4; p220 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213138 representing NM_020343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCCGAAGGAGCCACGGGGATGTGAAGAAGTCCACCCAGAAGGTGCTGGACCCGAAGAAGGACG TGCTGACCCGCCTGAAGCACCTGCGGGCGCTGCTGGATAATGTGGATGCAAATGATCTTAAGCAGTTTTT TGAGACCAACTATTCTCAGATATATTTCATCTTCTATGAAAATTTTATAGCACTGGAAAATAGTTTGAAA TTAAAAGGGAATAATAAGTCACAAAGGGAGGAGCTGGACTCCATCCTCTTCCTTTTTGAAAAAATACTGC AGTTTCTACCTGAACGAATTTTCTTTCGATGGCATTACCAGAGTATAGGCTCAACTTTAAAGAAGCTTCT ACACACTGGAAATTCTATTAAGATAAGATGTGAAGGAATCAGGCTGTTTCTTCTGTGGCTTCAAGCACTT CAGACAAACTGTGCAGAAGAGCAGGTTCTGATTTTTGCTTGCCTGGTGCCTGGTTTCCCAGCAGTCATGT CATCCAGGGGCCCTTGCACACTGGAGACACTCATCAATCCCAGCCCTAGTGTAGCTGATGTAAAGATATA TCCAGAAGAAATCACTCCACTCCTACCAGCCATATCAGGGGAGAAGATTGCTGAGGACCAAACCTGCTTT TTTCTTCAAATACTGTTGAAGTATATGGTTATTCAGGCTGCGAGCTTGGAGTGGAAGAATAAGGAGAATC AAGATACTGGTTTTAAATTTCTTTTTACATTGTTTCGAAAGTATTATCTTCCTCATTTATTTCCATCATT TACTAAGTTAACAAACATCTACAAACCTGTACTTGACATCCCCCATTTGAGACCAAAGCCTGTGTACATT ACTACCACTCGAGACAATGAGAACATTTACAGTACAAAGATTCCATATATGGCAGCTCGTGTTGTTTTTA TTAAGTGGATTGTAACCTTCTTTTTGGAAAAAAAGTATCTAACTGCAACACAAAACACTAAAAATGGAGT TGATGTATTGCCTAAAATCATCCAGACTGTTGGTGGTGGTGCTGTGCAGGAGAGAGCGCCTGAGCTGGAT GGTGGTGGGCCCACGGAGCAGGACAAAAGCCATTCTAACAGCAGCACCTTGTCGGACCGAAGACTCAGCA ACTCCAGCCTCTGTAGCATTGAAGAAGAGCACCGAATGGTGTATGAAATGGTACAGCGGATTCTCTTGTC AACACGAGGTTATGTCAACTTCGTGAATGAAGTATTTCACCAGGCATTTTTGTTGCCTTCCTGTGAGATA GCTGTAACAAGAAAAGTAGTTCAAGTGTACAGAAAGTGGATTCTCCAGGACAAACCTGTGTTCATGGAGG AGCCAGATAGAAAAGATGTTGCCCAAGAAGATGCTGAAAAATTAGGATTTTCCGAGACTGATAGCAAGGA GGCCTCATCTGAAAGTTCTGGTCATAAACGATCTTCCAGTTGGGGACGCACATACTCCTTCACAAGTGCA ATGAGCAGAGGGTGTGTGACAGAGGAGGAAAATACAAATGTGAAAGCCGGCGTCCAGGCTTTGTTGCAGG TATTTTTGACGAACTCTGCAAACATCTTTTTGTTGGAACCATGTGCTGAAGTTCCTGTGCTCTTGAAAGA ACAAGTTGATGCTTGTAAAGCTGTTTTGATTATTTTTAGGCGCATGATAATGGAGCTTACAATGAATAAA AAGACATGGGAACAGATGTTGCAAATACTACTCAGGATAACAGAAGCTGTCATGCAGAAGCCAAAGGATA AACAAATAAAGGACTTGTTTGCCCAGAGCTTGGCAGGGTTACTATTTAGGACGCTCATGGTAGCTTGGAT CCGAGCAAACCTCTGTGTGTACATTTCTCGAGAGCTCTGGGATGACTTTCTTGGTGTGCTGTCCTCCCTC ACGGAATGGGAGGAACTTATAAACGAGTGGGCCAACATTATGGACTCCTTGACAGCAGTGCTTGCAAGAA CCGTTTATGGAGTTGAGATGACAAACCTACCTCTGGATAAATTAAGTGAACAAAAGGAAAAGAAGCAACG AGGCAAAGGCTGTGTTCTAGATCCTCAGAAAGGAACCACCGTGGGGAGGTCCTTTTCTCTCAGCTGGCGG AGCCACCCAGATGTGACCGAACCGATGCGATTTAGGAGTGCCACCACGTCTGGAGCACCGGGAGTGGAAA AGGCAAGAAATATTGTTCGCCAAAAAGCAACTGAAGTGGAGGAGTGTCAACAGTCAGAAAATGCACCTGC AGCCGGATCTGGCCATCTCACAGTGGGACAGCAGCAGCAGGTCCTTCGGAGCAGCAGCACCTCCGACATC CCCGAGCCGCTGTGCTCAGATTCTTCTCAGGGTCAAAAGGCAGAAAACACACAGAATTCGAGTTCTTCAG AGCCTCAGCCTATTCAAGAGAATAAAGGACATGTGAAGAGAGAACATGAAGGAATAACAATCTTAGTTCG AAGAAGCAGCAGCCCTGCTGAATTGGATTTGAAAGATGATTTGCAGCAGACACAAGGAAAATGTAGGGAA AGACAGAAGAGTGAAAGTACTAACAGTGACACAACTCTGGGCTGTACCAATGAGGCAGAGCTGTCCATGG GCCCATGGCAGACCTGTGAGGAAGACCCAGAACTGAATACTCCCACAGATGTTGTGGCTGATGCTGATGC CCGTCATTGGTTACAACTGAGTCCCACCGATGCTTCAAATTTAACAGATAGCAGCGAGTGCCTCACAGAT GACTGTAGTATAATCGCCGGGGGGAGCCTCACTGGTTGGCACCCAGACTCTGCTGCTGTGTTATGGCGAA GGGTCTTGGGGATCCTCGGAGATGTGAATAACATCCAGTCACCCAAGATCCACGCCAGAGTTTTCTGCTA TCTCTATGAACTCTGGTACAAACTAGCAAAGATACGGGATAATCTAGCAATAAGCCTGGATAACCAGTCT TCTCCATCTCCTCCAGTTTTGATCCCACCACTCAGAATGTTTGCATCATGGCTGTTTAAGGCGGCGACAC TGCCAAATGAATATAAGGAAGGCAAACTACAAGCCTACAGGCTGATCTGTGCCATGATGACCAGACGCCA GGACGTTCTGCCAAACTCAGATTTCCTGGTGCATTTTTACCTTGTGATGCACCTGGGATTAACCAGCGAG GATCAGGATATCTTAAATACGATCATAAGGCACTGTCCACCCCGCTTTTTCTCCCTGGGTTTTCCTGGCT TCTCAATGCTGGTGGGGGACTTCATCACGGCCGCTGCCAGGGTCCTTAGCACAGACATTTTGACGGCGCC TCGTTCAGAGGCTGTCACTGTCCTCGGCTCTCTGGTCTGCTTTCCAAATACCTACCAGGAGATTCCTTTA CTGCAGTCAGTGCCAGAAGTAAATGAGGCCATTACAGGAACTGAAGATGTCAAGCATTACCTCATAAATA TTTTACTGAAGAATGCCACAGAAGAACCAAATGAATATGCAAGATGCATTGCTGTTTGCTCCCTTGGGGT CTGGATATGTGAAGAGCTTGCACAGTGTACAAGCCACCCTCAGGTGAAAGAGGCCATCAATGTGATAGGA GTAACTCTGAAGTTTCCCAACAAAATCGTGGCCCAGGTAGCTTGCGATGTCCTTCAGTTGCTGGTTTCCT ACTGGGAGAAGCTTCAGATGTTTGAAACCTCTCTGCCTCGGAAAATGGCAGAAATCCTCGTGGCCACAGT TGCTTTTCTTTTACCAAGTGCAGAGTACTCCTCAGTGGAAACAGACAAGAAGTTTATTGTGTCCTTGCTA CTCTGCCTCTTGGACTGGTGCATGGCATTGCCCGTGAGTGTCCTTCTCCACCCCGTGTCCACAGCAGTCC TAGAGGAGCAGCATTCGGCCAGAGCCCCCTTGCTGGATTATATCTACAGGGTTTTGCACTGCTGTGTGTG TGGCTCAAGCACGTACACCCAACAGAGTCACTACATACTGACCCTGGCTGACTTGTCATCCACGGATTAT GACCCCTTCCTGCCACTGGCAAATGTGAAGAGCTCTGAGCCAGTCCAGTATCATTCATCAGCAGAATTGG GTAACCTGCTGACTGTTGAAGAGGAGAAGAAGAGAAGAAGTTTGGAGTTGATCCCTTTGACTGCTCGAAT GGTGATGGCCCACCTGGTGAACCACCTGGGGCACTACCCCCTCAGTGGGGGCCCTGCCATACTGCACAGC CTTGTCAGCGAGAACCATGACAATGCCCATGTGGAAGGCTCCGAGCTGTCCTTTGAGGTGTTCAGAAGTC CAAACCTGCAGCTGTTTGTATTTAATGATAGCACCCTCATCTCCTACCTTCAGACACCCACAGAAGGACC GGTAGGGGGATCACCAGTGGGCTCTCTCTCTGATGTGAGAGTAATTGTGAGGGATATCTCAGGGAAGTAC TCTTGGGATGGTAAGGTTTTATATGGACCTTTGGAAGGCTGCTTAGCACCCAGTGGAAGAAATCCTTCAT TTCTGATTTCGAGCTGGCATCGTGACACATTTGGACCTCAGAAAGACTCTTCTCAAGTTGAGGAGGGGGA TGATGTTCTTGACAAATTACTTGAAAACATTGGCCATACAAGTCCTGAATGCCTTTTACCGTCACAGCTA AATCTAAATGAACCTTCCCTAACCCCATGTGGCATGAACTATGACCAAGAGAAGGAAATCATTGAGGTCA TTTTGCGCCAAAATGCTCAAGAGGATGAGTATATCCAGAGTCATAACTTCGATTCTGCAATGAAAGTCAC CAGCCAAGGGCAGCCCTCCCCAGTGGAGCCCCGAGGACCCTTTTATTTCTGCAGGTTATTGCTTGATGAC TTGGGAATGAATTCTTGGGACAGAAGGAAGAATTTTCATCTATTGAAGAAAAATTCAAAATTATTGAGAG AGCTGAAAAATTTGGACTCCCGCCAGTGCCGTGAGACACACAAAATCGCAGTGTTTTACATTGCTGAAGG TCAAGAAGACAAGTGTTCAATCCTCTCTAATGAAAGAGGAAGCCAAGCATATGAAGACTTTGTTGCTGGA CTTGGATGGGAGGTGGATCTCTCCACCCACTGTGGGTTCATGGGTGGCCTTCAGCGCAATGGCAGCACCG GGCAGACGGCCCCTTACTATGCTACCTCAACTGTGGAAGTGATTTTCCATGTTTCCACTCGAATGCCGTC AGACTCAGATGATTCCCTCACCAAAAAGCTTCGTCACTTGGGGAATGACGAGGTCCATATCGTCTGGTCT GAACACTCCAGAGACTACCGCAGGGGTATTATCCCAACTGCCTTTGGAGATGTTTCAATCATTATTTACC CAATGAAGAATCACATGTTCTTCATCGCGATAACGAAGAAACCTGAGGTTCCATTTTTTGGGCCTCTGTT TGATGGAGCCATAGTGAGTGGGAAGCTGCTGCCAAGCCTTGTATGTGCCACGTGCATCAACGCCAGCAGG GCTGTGAAGTGCCTCATCCCACTCTACCAGAGCTTCTATGAAGAGCGAGCTCTGTATCTCGAAGCAATAA TTCAGAACCACCGCGAAGTAATGACATTCGAGGATTTCGCAGCCCAAGTCTTTTCTCCCTCTCCCAGCTA CTCCCTCAGCGGAACGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213138 representing NM_020343
Red=Cloning site Green=Tags(s) MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDANDLKQFFETNYSQIYFIFYENFIALENSLK LKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQAL QTNCAEEQVLIFACLVPGFPAVMSSRGPCTLETLINPSPSVADVKIYPEEITPLLPAISGEKIAEDQTCF FLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLDIPHLRPKPVYI TTTRDNENIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAVQERAPELD GGGPTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRMVYEMVQRILLSTRGYVNFVNEVFHQAFLLPSCEI AVTRKVVQVYRKWILQDKPVFMEEPDRKDVAQEDAEKLGFSETDSKEASSESSGHKRSSSWGRTYSFTSA MSRGCVTEEENTNVKAGVQALLQVFLTNSANIFLLEPCAEVPVLLKEQVDACKAVLIIFRRMIMELTMNK KTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLMVAWIRANLCVYISRELWDDFLGVLSSL TEWEELINEWANIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVLDPQKGTTVGRSFSLSWR SHPDVTEPMRFRSATTSGAPGVEKARNIVRQKATEVEECQQSENAPAAGSGHLTVGQQQQVLRSSSTSDI PEPLCSDSSQGQKAENTQNSSSSEPQPIQENKGHVKREHEGITILVRRSSSPAELDLKDDLQQTQGKCRE RQKSESTNSDTTLGCTNEAELSMGPWQTCEEDPELNTPTDVVADADARHWLQLSPTDASNLTDSSECLTD DCSIIAGGSLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHARVFCYLYELWYKLAKIRDNLAISLDNQS SPSPPVLIPPLRMFASWLFKAATLPNEYKEGKLQAYRLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSE DQDILNTIIRHCPPRFFSLGFPGFSMLVGDFITAAARVLSTDILTAPRSEAVTVLGSLVCFPNTYQEIPL LQSVPEVNEAITGTEDVKHYLINILLKNATEEPNEYARCIAVCSLGVWICEELAQCTSHPQVKEAINVIG VTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKMAEILVATVAFLLPSAEYSSVETDKKFIVSLL LCLLDWCMALPVSVLLHPVSTAVLEEQHSARAPLLDYIYRVLHCCVCGSSTYTQQSHYILTLADLSSTDY DPFLPLANVKSSEPVQYHSSAELGNLLTVEEEKKRRSLELIPLTARMVMAHLVNHLGHYPLSGGPAILHS LVSENHDNAHVEGSELSFEVFRSPNLQLFVFNDSTLISYLQTPTEGPVGGSPVGSLSDVRVIVRDISGKY SWDGKVLYGPLEGCLAPSGRNPSFLISSWHRDTFGPQKDSSQVEEGDDVLDKLLENIGHTSPECLLPSQL NLNEPSLTPCGMNYDQEKEIIEVILRQNAQEDEYIQSHNFDSAMKVTSQGQPSPVEPRGPFYFCRLLLDD LGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAG LGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWS EHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLVCATCINASR AVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSLSGTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020343 |
| ORF Size | 5619 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020343.1, NP_065076.1 |
| RefSeq Size | 5628 bp |
| RefSeq ORF | 5622 bp |
| Locus ID | 57186 |
| UniProt ID | Q2PPJ7 |
| Gene Summary | Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213138 | RALGAPA2 (Myc-DDK-tagged)-Human Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2) |
CNY 19,360.00 |
|
| RC213138L1 | Lenti ORF clone of Human Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), Myc-DDK-tagged |
CNY 20,890.00 |
|
| SC304744 | RALGAPA2 (untagged)-Human Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2) |
CNY 36,010.00 |
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