USP32 (NM_032582) Human Tagged ORF Clone
CAT#: RG212770
- TrueORF®
USP32 (tGFP-tagged) - Human ubiquitin specific peptidase 32 (USP32)
ORF Plasmid: DDK
"NM_032582" in other vectors (6)
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CNY 18,056.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | NY-REN-60; USP10 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG212770 representing NM_032582
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCCAAGGAGTCACGGATCGGATTCCTCAGCTACGAGGAGGCGCTGAGGAGAGTTACAGATGTAG AGCTAAAACGACTGAAGGATGCTTTCAAGAGGACCTGTGGACTCTCATATTACATGGGCCAGCACTGCTT CATCCGGGAAGTGCTTGGGGATGGAGTGCCTCCAAAGGTTGCTGAGGTGATTTACTGTTCTTTTGGTGGA ACATCCAAAGGGCTGCACTTCAATAATTTAATAGTTGGACTTGTCCTCCTTACAAGAGGCAAAGATGAAG AGAAAGCAAAATACATTTTTAGTCTTTTTTCAAGTGAATCTGGGAACTATGTTATACGGGAAGAAATGGA AAGAATGCTCCACGTGGTGGATGGTAAAGTCCCAGATACACTCAGGAAGTGTTTCTCAGAGGGTGAAAAG GTAAACTATGAAAAGTTTAGAAATTGGCTTTTTCTAAACAAAGATGCTTTTACTTTCTCTCGATGGCTTC TATCTGGAGGTGTGTATGTTACCCTCACTGATGATAGTGATACTCCTACTTTCTACCAAACTCTGGCTGG AGTCACACATTTGGAGGAATCAGACATCATTGATCTTGAGAAACGCTATTGGTTATTGAAGGCTCAATCC CGGACTGGACGATTTGATTTAGAGACATTTGGCCCATTGGTTTCACCACCTATTCGTCCATCTCTAAGTG AAGGTTTGTTTAATGCTTTTGATGAAAATCGTGACAATCACATAGATTTTAAGGAGATATCCTGTGGGTT ATCAGCCTGTTGCAGGGGACCCCTGGCTGAAAGACAAAAATTTTGCTTCAAGGTATTTGATGTTGACCGT GATGGAGTTCTCTCCAGGGTTGAACTGAGAGACATGGTGGTTGCACTTTTAGAAGTCTGGAAGGACAACC GCACTGATGATATTCCTGAATTACATATGGATCTCTCTGATATTGTAGAAGGCATACTGAATGCACATGA CACCACAAAGATGGGTCATCTTACTCTGGAAGACTATCAGATCTGGAGTGTGAAAAATGTTCTTGCCAAT GAGTTTTTGAACCTCCTTTTCCAGGTGTGTCACATAGTTCTGGGGTTAAGACCAGCTACTCCGGAAGAAG AAGGACAAATTATTAGAGGATGGTTAGAACGAGAGAGCAGGTATGGTCTGCAAGCAGGACACAACTGGTT TATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATACGATGCCAACCCTGTGGTAATT GAGCCATCATCTGTTTTGAATGGAGGAAAATACTCATTTGGAACTGCAGCCCATCCTATGGAGCAGGTCG AAGATAGAATTGGAAGCAGCCTCAGTTACGTGAATACTACAGAAGAGAAATTTTCAGACAACATTTCTAC TGCATCTGAAGCCTCAGAAACTGCTGGCAGCGGCTTTCTGTATTCTGCCACACCAGGGGCAGATGTTTGC TTTGCTCGACAACATAACACTTCTGACAATAACAACCAGTGTTTGCTGGGAGCCAATGGGAATATTTTGT TGCACCTTAACCCTCAGAAACCAGGGGCTATTGATAATCAGCCATTAGTAACTCAAGAACCAGTAAAGGC TACATCATTAACACTAGAAGGAGGACGATTAAAACGAACTCCACAGCTGATTCATGGAAGAGACTATGAA ATGGTCCCAGAACCTGTGTGGAGAGCACTTTATCACTGGTATGGAGCAAACCTGGCCTTACCTAGACCAG TTATCAAGAACAGCAAGACAGACATCCCAGAGCTGGAATTATTTCCCCGCTATCTTCTCTTCCTGAGACA GCAGCCTGCCACTCGGACACAGCAGTCTAACATCTGGGTGAATATGGGAAATGTACCTTCTCCGAATGCA CCTTTAAAGCGGGTATTAGCCTATACAGGCTGTTTTAGTCGAATGCAGACCATCAAGGAAATTCACGAAT ATCTATCTCAAAGGCTGCGCATTAAAGAGGAAGATATGCGCCTGTGGCTATACAACAGTGAGAACTACCT TACTCTTCTGGATGATGAGGATCATAAATTGGAATATTTGAAAATCCAGGATGAACAACACCTGGTAATT GAAGTTCGCAACAAAGATATGAGTTGGCCTGAGGAGATGTCTTTTATAGCAAATAGTAGTAAAATAGATA GACACAAGGTTCCCACAGAAAAGGGAGCCACAGGTCTAAGCAATCTGGGAAACACATGCTTCATGAACTC AAGCATCCAGTGTGTTAGTAACACACAGCCACTGACACAGTATTTTATCTCAGGGAGACATCTTTATGAA CTCAACAGGACAAATCCCATTGGTATGAAGGGGCATATGGCTAAATGCTATGGTGATTTAGTGCAGGAAC TTTGGAGTGGAACTCAGAAGAATGTTGCCCCATTAAAGCTTCGGTGGACCATAGCAAAATATGCTCCCAG GTTTAATGGGTTTCAGCAACAGGACTCCCAAGAACTTCTGGCTTTTCTCTTGGATGGTCTTCATGAAGAT CTTAATCGAGTCCATGAAAAGCCATATGTGGAACTGAAGGACAGTGATGGGCGACCAGACTGGGAAGTAG CTGCAGAGGCCTGGGACAACCATCTAAGAAGAAATAGATCAATTGTTGTGGATTTGTTCCATGGGCAGCT AAGATCTCAAGTAAAATGCAAGACATGTGGGCATATAAGTGTCCGATTTGACCCTTTCAATTTTTTGTCT TTGCCACTACCAATGGACAGTTATATGCACTTAGAAATAACAGTGATTAAGTTAGATGGTACTACCCCTG TACGGTATGGACTAAGACTGAATATGGATGAAAAGTACACAGGTTTAAAAAAACAGCTGAGTGATCTCTG TGGACTTAATTCAGAACAAATCCTTCTAGCAGAAGTACATGGTTCCAACATAAAGAACTTTCCTCAGGAC AACCAAAAAGTACGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTGTCCCTGTGTCTCCAA TTTCAGCTTCTAGTCCAACACAGACAGATTTCTCCTCTTCGCCATCTACAAATGAAATGTTCACCCTAAC TACCAATGGGGACCTACCCCGACCAATATTCATCCCCAATGGAATGCCAAACACTGTTGTGCCATGTGGA ACTGAGAAGAACTTCACAAATGGAATGGTTAATGGTCACATGCCATCTCTTCCTGACAGCCCCTTTACAG GTTACATCATTGCAGTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCATCTCAGAAGAATCG CCCCAGCCTCTTTGGAATGCCATTGATTGTTCCATGTACTGTGCATACCCGGAAGAAAGACCTATATGAT GCGGTTTGGATTCAAGTATCCCGGTTAGCGAGCCCACTCCCACCTCAGGAAGCTAGTAATCATGCCCAGG ATTGTGACGACAGTATGGGCTATCAATATCCATTCACTCTACGAGTTGTGCAGAAAGATGGGAACTCCTG TGCTTGGTGCCCATGGTATAGATTTTGCAGAGGCTGTAAAATTGATTGTGGGGAAGACAGAGCTTTCATT GGAAATGCCTATATCGCTGTGGATTGGGATCCCACAGCCCTTCACCTTCGCTATCAAACATCCCAGGAAA GGGTTGTAGATGAGCATGAGAGTGTGGAGCAGAGTCGGCGAGCGCAAGCCGAGCCCATCAACCTGGACAG CTGTCTCCGTGCTTTCACCAGTGAGGAAGAGCTAGGGGAAAATGAGATGTACTACTGTTCCAAGTGTAAG ACCCACTGCTTAGCAACAAAGAAGCTGGATCTCTGGAGGCTTCCACCCATCCTGATTATTCACCTTAAGC GATTTCAATTTGTAAATGGTCGGTGGATAAAATCACAGAAAATTGTCAAATTTCCTCGGGAAAGTTTTGA TCCAAGTGCTTTTTTGGTACCAAGAGACCCGGCTCTCTGCCAGCATAAACCACTCACACCCCAGGGGGAT GAGCTCTCTGAGCCCAGGATTCTGGCAAGGGAGGTGAAGAAAGTGGATGCGCAGAGTTCGGCTGGGGAAG AGGACGTGCTCCTGAGCAAAAGCCCATCCTCACTCAGCGCTAACATCATCAGCAGCCCGAAAGGTTCTCC TTCTTCATCAAGAAAAAGTGGAACCAGCTGTCCCTCCAGCAAAAACAGCAGCCCTAATAGCAGCCCACGG ACTTTGGGGAGGAGCAAAGGGAGGCTCCGGCTGCCCCAGATTGGCAGCAAAAATAAACTGTCAAGTAGTA AAGAGAACTTGGATGCCAGCAAAGAAAATGGGGCTGGGCAGATATGTGAGCTGGCTGACGCCTTGAGTCG AGGGCATGTGCTGGGGGGCAGCCAACCAGAGTTGGTCACTCCTCAGGACCATGAGGTAGCTTTGGCCAAT GGATTCCTTTATGAGCATGAAGCATGTGGCAATGGCTACAGCAATGGTCAGCTTGGAAACCACAGTGAAG AAGACAGCACTGATGACCAAAGAGAAGATACTCGTATTAAGCCTATTTATAATCTATATGCAATTTCGTG CCATTCAGGAATTCTGGGTGGGGGCCATTACGTCACTTATGCCAAAAACCCAAACTGCAAGTGGTACTGT TACAATGACAGCAGCTGTAAGGAACTTCACCCGGATGAAATTGACACCGACTCTGCCTACATTCTTTTCT ATGAGCAGCAGGGGATAGACTATGCACAATTTCTGCCAAAGACTGATGGCAAAAAGATGGCAGACACAAG CAGTATGGATGAAGACTTTGAGTCTGATTACAAAAAGTACTGTGTGTTACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212770 representing NM_032582
Red=Cloning site Green=Tags(s) MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMGQHCFIREVLGDGVPPKVAEVIYCSFGG TSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGNYVIREEMERMLHVVDGKVPDTLRKCFSEGEK VNYEKFRNWLFLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQS RTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDR DGVLSRVELRDMVVALLEVWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVLAN EFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFIISMQWWQQWKEYVKYDANPVVI EPSSVLNGGKYSFGTAAHPMEQVEDRIGSSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVC FARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYE MVPEPVWRALYHWYGANLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNA PLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHLVI EVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYE LNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHED LNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLS LPLPMDSYMHLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLSDLCGLNSEQILLAEVHGSNIKNFPQD NQKVRLSVSGFLCAFEIPVPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVPCG TEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYD AVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFI GNAYIAVDWDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCK THCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPALCQHKPLTPQGD ELSEPRILAREVKKVDAQSSAGEEDVLLSKSPSSLSANIISSPKGSPSSSRKSGTSCPSSKNSSPNSSPR TLGRSKGRLRLPQIGSKNKLSSSKENLDASKENGAGQICELADALSRGHVLGGSQPELVTPQDHEVALAN GFLYEHEACGNGYSNGQLGNHSEEDSTDDQREDTRIKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYC YNDSSCKELHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSSMDEDFESDYKKYCVLQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_032582 |
ORF Size | 4812 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032582.4 |
RefSeq Size | 7026 bp |
RefSeq ORF | 4815 bp |
Locus ID | 84669 |
UniProt ID | Q8NFA0 |
Domains | UCH, EFh, DUSP |
Protein Families | Druggable Genome, Protease |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212770 | USP32 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 32 (USP32) |
CNY 16,456.00 |
|
RC212770L1 | Lenti-ORF clone of USP32 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 32 (USP32) |
CNY 18,856.00 |
|
RC212770L2 | Lenti-ORF clone of USP32 (mGFP-tagged)-Human ubiquitin specific peptidase 32 (USP32) |
CNY 15,680.00 |
|
RC212770L3 | Lenti-ORF clone of USP32 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 32 (USP32) |
CNY 15,680.00 |
|
RC212770L4 | Lenti-ORF clone of USP32 (mGFP-tagged)-Human ubiquitin specific peptidase 32 (USP32) |
CNY 15,680.00 |
|
SC108152 | USP32 (untagged)-Human ubiquitin specific peptidase 32 (USP32) |
CNY 16,472.00 |