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SMARCA6 (HELLS) (NM_018063) Human Tagged ORF Clone
CAT＃: RG212231
- TrueORF®
HELLS (tGFP-tagged) - Human helicase, lymphoid-specific (HELLS)
ORF Plasmid: DDK
"NM_018063" in other vectors (4)
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CNY 7,736.00
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ICF4; LSH; Nbla10143; PASG; SMARCA6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212231 representing NM_018063
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCGGAACGGCCCGCGGGCAGCGGCGGCTCGGAGGCTCCAGCAATGGTTGAACAACTGGACACTG CTGTGATTACCCCGGCCATGCTAGAAGAGGAAGAACAGCTTGAAGCTGCTGGACTAGAGAGAGAGCGGAA GATGCTGGAAAAGGCTCGCATGTCTTGGGATAGAGAGTCGACAGAAATTCGGTACCGTAGACTTCAACAT TTGCTTGAAAAAAGCAATATATACTCCAAATTTTTATTGACGAAAATGGAACAGCAACAATTAGAGGAAC AGAAGAAGAAAGAAAAATTGGAGAGAAAAAAGGAGTCTTTAAAAGTTAAAAAGGGTAAAAATTCAATTGA TGCAAGTGAAGAGAAGCCAGTTATGAGGAAAAAAAGAGGAAGAGAAGATGAATCATACAATATTTCAGAG GTCATGTCAAAAGAGGAAATTTTGTCTGTGGCTAAAAAAAATAAAAAGGAGAATGAGGATGAAAACTCCT CCTCTACTAATCTCTGTGTGGAAGATCTTCAGAAAAATAAAGATTCGAATAGTATAATTAAAGATAGATT GTCTGAAACGGTTAGGCAGAATACTAAATTCTTTTTTGACCCAGTCCGGAAGTGTAATGGTCAGCCAGTA CCTTTTCAACAACCAAAGCACTTCACTGGAGGAGTGATGCGATGGTACCAAGTAGAAGGCATGGAATGGC TTAGGATGCTTTGGGAAAATGGAATTAATGGCATTTTAGCAGATGAAATGGGATTGGGTAAGACAGTTCA GTGCATTGCTACTATTGCATTGATGATTCAGAGAGGAGTACCAGGACCTTTTCTTGTCTGTGGCCCTTTG TCTACACTTCCTAACTGGATGGCTGAATTCAAAAGATTTACACCAGATATCCCTACAATGTTATATCATG GAACCCAGGAGGAACGTCAAAAATTGGTAAGAAATATTTACAAACGGAAAGGGACTTTGCAGATTCATCC TGTGGTAATCACGTCATTTGAAATAGCCATGAGAGACCGAAATGCGTTACAGCATTGCTATTGGAAATAC TTAATAGTAGATGAAGGACACAGGATTAAGAATATGAAGTGCCGTCTAATCAGGGAGTTAAAACGATTCA ATGCTGATAACAAACTTCTTTTGACTGGTACTCCCTTGCAAAACAATTTATCAGAACTTTGGTCATTGCT AAACTTTTTGTTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTCTTGGTTTGACATCACTAGTCTT TCTGAAACTGCTGAAGATATTATTGCTAAAGAAAGAGAACAGAATGTATTGCATATGCTGCACCAGATTT TAACACCTTTCTTATTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAGTTCCTCCTAAACGAGAAGTAGT CGTTTATGCTCCACTTTCAAAGAAGCAGGAGATCTTTTATACAGCCATTGTGAACCGTACAATTGCAAAC ATGTTTGGATCCAGTGAGAAAGAAACAATTGAGTTAAGTCCTACTGGTCGACCAAAACGACGAACTAGAA AATCAATAAATTACAGCAAAATAGATGATTTCCCTAATGAATTGGAAAAACTGATCAGTCAAATACAGCC AGAGGTGGACCGAGAAAGAGCTGTTGTGGAAGTGAATATCCCTGTAGAATCTGAAGTTAATCTGAAGCTG CAGAATATAATGATGCTACTTCGTAAATGTTGTAATCATCCATATTTGATTGAATATCCTATAGACCCTG TTACACAAGAATTTAAGATCGATGAAGAATTGGTAACAAATTCTGGGAAGTTCTTGATTTTGGATCGAAT GCTGCCAGAACTAAAAAAAAGAGGTCACAAGGTGCTGCTTTTTTCACAAATGACAAGCATGTTGGACATT TTGATGGATTACTGCCATCTCAGAGATTTCAACTTCAGCAGGCTTGATGGGTCCATGTCTTACTCAGAGA GAGAAAAAAACATGCACAGCTTCAACACGGATCCAGAGGTGTTTATCTTCTTAGTGAGTACACGAGCTGG TGGCCTGGGCATTAATCTGACTGCAGCAGATACAGTTATCATTTATGATAGTGATTGGAACCCCCAGTCG GATCTTCAGGCCCAGGATAGATGTCATAGAATTGGTCAGACAAAGCCAGTTGTTGTTTATCGCCTTGTTA CAGCAAATACTATCGATCAGAAAATTGTGGAAAGAGCAGCTGCTAAAAGGAAACTGGAAAAGTTGATCAT CCATAAAAATCATTTCAAAGGTGGTCAGTCTGGATTAAATCTGTCTAAGAATTTCTTAGATCCTAAGGAA TTAATGGAATTATTAAAATCTAGAGATTATGAAAGGGAAATAAAAGGATCAAGAGAGAAGGTCATTAGTG ATAAAGATCTAGAGTTGTTGTTAGATCGAAGTGATCTTATTGATCAAATGAATGCTTCAGGACCAATTAA AGAGAAGATGGGGATATTCAAGATATTAGAAAATTCTGAAGATTCCAGTCCTGAATGTTTGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212231 representing NM_018063
Red=Cloning site Green=Tags(s) MPAERPAGSGGSEAPAMVEQLDTAVITPAMLEEEEQLEAAGLERERKMLEKARMSWDRESTEIRYRRLQH LLEKSNIYSKFLLTKMEQQQLEEQKKKEKLERKKESLKVKKGKNSIDASEEKPVMRKKRGREDESYNISE VMSKEEILSVAKKNKKENEDENSSSTNLCVEDLQKNKDSNSIIKDRLSETVRQNTKFFFDPVRKCNGQPV PFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPL STLPNWMAEFKRFTPDIPTMLYHGTQEERQKLVRNIYKRKGTLQIHPVVITSFEIAMRDRNALQHCYWKY LIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSL SETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFYTAIVNRTIAN MFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKL QNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDI LMDYCHLRDFNFSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQS DLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKE LMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018063 |
| ORF Size | 2514 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018063.5 |
| RefSeq Size | 3165 bp |
| RefSeq ORF | 2517 bp |
| Locus ID | 3070 |
| UniProt ID | Q9NRZ9 |
| Domains | SNF2_N, DEAD, helicase_C |
| Gene Summary | This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212231 | HELLS (Myc-DDK-tagged)-Human helicase, lymphoid-specific (HELLS) |
CNY 6,136.00 |
|
| RC212231L3 | Lenti ORF clone of Human helicase, lymphoid-specific (HELLS), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC212231L4 | Lenti ORF clone of Human helicase, lymphoid-specific (HELLS), mGFP tagged |
CNY 9,030.00 |
|
| SC126963 | HELLS (untagged)-Human helicase, lymphoid-specific (HELLS) |
CNY 6,144.00 |
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