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ZNF93 (NM_031218) Human Tagged ORF Clone
CAT＃: RG212079
- TrueORF®
ZNF93 (tGFP-tagged) - Human zinc finger protein 93 (ZNF93). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_031218" in other vectors (2)
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CNY 4,370.00
货期*
3周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HPF34; HTF34; TF34; ZNF505 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212079 ORF sequence, codon optimized.
Due to the complexity of NM_031218, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCCCTTCAGTTCCGGGACGTGGCCATCGAGTTCTCTCTCGAGGAATGGCATTGCCTGGATACCG CACAGAGAAATCTCTACCGGAACGTTATGCTCGAAAACTACAGCAACCTCGTGTTCCTGGGTATCGTCGT TTCTAAGCCGGACCTTATCGCGCATTTGGAACAGGGGAAAAAACCACTGACAATGAAACGACACGAGATG GTGGCGAATCCGAGTGTGATTTGCTCCCACTTCGCCCAAGATCTGTGGCCCGAACAGAATATCAAGGATT CTTTTCAGAAGGTCATCCTTCGGCGATACGAGAAAAGGGGCCACGGGAATCTGCAACTGATTAAACGGTG CGAAAGCGTGGACGAGTGCAAAGTTCACACTGGGGGCTACAATGGTCTGAACCAATGTAGCACGACCACC CAAAGCAAGGTGTTCCAGTGCGATAAGTACGGCAAGGTGTTTCACAAGTTTTCCAACTCTAACCGCCATA ATATCCGGCACACCGAGAAGAAGCCATTCAAATGTATAGAATGCGGCAAGGCCTTCAACCAGTTTAGCAC ACTCATCACACATAAAAAAATCCACACGGGCGAGAAGCCGTATATATGTGAAGAGTGTGGCAAGGCGTTT AAGTACTCAAGTGCTCTCAATACCCACAAAAGGATACATACTGGCGAAAAACCATATAAATGTGATAAGT GTGATAAGGCCTTTATCGCCTCCAGCACATTGTCAAAACATGAGATTATCCACACGGGAAAGAAGCCATA CAAATGCGAAGAATGCGGCAAAGCTTTTAACCAGTCCAGCACCCTGACAAAACACAAGAAAATTCATACT GGTGAAAAACCATACAAGTGCGAGGAGTGCGGTAAAGCTTTTAACCAATCCAGTACCTTGACTAAGCATA AGAAGATCCACACTGGAGAAAAACCCTACGTGTGTGAAGAGTGTGGCAAGGCATTTAAATACTCTCGAAT TCTCACAACACATAAGAGAATTCATACCGGCGAAAAGCCGTACAAATGCAACAAATGCGGAAAGGCATTC ATCGCCAGTTCTACTCTTAGCCGCCACGAATTTATTCACATGGGAAAGAAGCATTATAAATGCGAGGAGT GCGGGAAGGCCTTCATATGGAGCTCTGTGCTGACAAGGCATAAGCGGGTGCACACCGGTGAAAAGCCCTA TAAATGTGAGGAGTGCGGCAAAGCGTTTAAATACAGTTCTACACTTAGTAGCCATAAACGATCACATACA GGAGAGAAACCGTACAAGTGCGAAGAATGCGGGAAAGCCTTCGTCGCCTCATCCACTCTTAGCAAGCACG AGATCATCCATACAGGAAAAAAGCCATACAAGTGTGAAGAATGTGGAAAAGCGTTTAATCAGAGTAGTAG CCTGACCAAGCATAAGAAAATCCATACGGGCGAGAAGCCCTATAAATGCGAAGAGTGCGGCAAGGCTTTC AATCAGAGCTCCTCTCTGACCAAGCATAAAAAAATTCATACAGGCGAGAAGCCATATAAGTGTGAGGAGT GCGGAAAAGCCTTTAACCAGAGTAGCACTCTCATAAAGCATAAGAAGATCCATACACGCGAGAAACCCTA CAAGTGTGAAGAGTGCGGTAAAGCATTCCACCTGTCTACTCACCTGACCACCCATAAGATACTTCATACC GGCGAGAAGCCTTATCGATGTAGGGAGTGTGGGAAGGCCTTTAATCACAGCGCAACACTTAGTAGCCATA AAAAGATACACTCAGGCGAAAAGCCATACGAGTGTGATAAATGCGGGAAAGCATTCATCTCCCCTAGTTC TCTGTCTCGGCATGAAATCATCCATACCGGCGAAAAACCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212079 representing NM_031218
Red=Cloning site Green=Tags(s) MGPLQFRDVAIEFSLEEWHCLDTAQRNLYRNVMLENYSNLVFLGIVVSKPDLIAHLEQGKKPLTMKRHEM VANPSVICSHFAQDLWPEQNIKDSFQKVILRRYEKRGHGNLQLIKRCESVDECKVHTGGYNGLNQCSTTT QSKVFQCDKYGKVFHKFSNSNRHNIRHTEKKPFKCIECGKAFNQFSTLITHKKIHTGEKPYICEECGKAF KYSSALNTHKRIHTGEKPYKCDKCDKAFIASSTLSKHEIIHTGKKPYKCEECGKAFNQSSTLTKHKKIHT GEKPYKCEECGKAFNQSSTLTKHKKIHTGEKPYVCEECGKAFKYSRILTTHKRIHTGEKPYKCNKCGKAF IASSTLSRHEFIHMGKKHYKCEECGKAFIWSSVLTRHKRVHTGEKPYKCEECGKAFKYSSTLSSHKRSHT GEKPYKCEECGKAFVASSTLSKHEIIHTGKKPYKCEECGKAFNQSSSLTKHKKIHTGEKPYKCEECGKAF NQSSSLTKHKKIHTGEKPYKCEECGKAFNQSSTLIKHKKIHTREKPYKCEECGKAFHLSTHLTTHKILHT GEKPYRCRECGKAFNHSATLSSHKKIHSGEKPYECDKCGKAFISPSSLSRHEIIHTGEKP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031218 |
| ORF Size | 1860 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031218.2, NP_112495.2 |
| RefSeq Size | 2794 bp |
| RefSeq ORF | 1863 bp |
| Locus ID | 81931 |
| UniProt ID | P35789 |
| Protein Families | Transcription Factors |
| Gene Summary | Transcription factor specifically required to repress long interspersed nuclear element 1 (L1) retrotransposons: recognizes and binds L1 sequences and repress their expression by recruiting a repressive complex containing TRIM28/KAP1 (PubMed:25274305). Not able to repress expression of all subtypes of L1 elements. Binds to the 5' end of L1PA4, L1PA5 and L1PA6 subtypes, and some L1PA3 subtypes. Does not bind to L1PA7 or older subtypes nor at the most recently evolved L1PA2 and L1Hs. 50% of L1PA3 elements have lost the ZNF93-binding site, explaining why ZNF93 is not able to repress their expression (PubMed:25274305).[UniProtKB/Swiss-Prot Function] |
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