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AFF2 (NM_002025) Human Tagged ORF Clone
CAT＃: RG212045
- TrueORF®
AFF2 (tGFP-tagged) - Human AF4/FMR2 family, member 2 (AFF2), transcript variant 1
ORF Plasmid: DDK
"NM_002025" in other vectors (4)
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CNY 10,728.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212045 representing NM_002025
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTATTCGACTTTTTCAGAGACTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTG CACTTAAAAAAAGGGAATGGGAGCGGAGGAATCAAGAAGTCCAGCAAGAAGACGATCTCTTTTCTTCAGG CTTTGATCTTTTTGGGGAGCCATACAAGGTAGCTGAATATACAAACAAAGGTGATGCACTTGCCAACCGA GTCCAGAACACGCTTGGAAACTATGATGAAATGAAGAATTTGCTAACTAACCATTCTAATCAGAATCACC TAGTGGGAATTCCAAAGAATTCTGTGCCCCAGAATCCCAACAACAAAAATGAACCAAGCTTTTTTCCAGA ACAAAAGAACAGAATAATTCCACCTCACCAGGATAATACCCATCCTTCAGCACCAATGCCTCCACCTTCT GTTGTGATACTGAATTCAACTCTAATACACAGCAACAGAAAATCAAAACCTGAGTGGTCACGTGATAGTC ATAACCCTAGCACTGTACTGGCAAGCCAGGCCAGTGGTCAGCCAAACAAGATGCAGACTTTGACACAGGA CCAGTCTCAAGCCAAACTGGAAGACTTCTTTGTCTACCCAGCTGAACAGCCCCAGATTGGAGAAGTTGAA GAGTCAAACCCATCTGCAAAGGAAGACAGTAACCCTAATTCTAGTGGAGAAGATGCTTTCAAAGAAATCT TTCAATCCAATTCACCGGAAGAATCTGAATTCGCCGTGCAAGCGCCTGGGTCTCCCCTAGTGGCTTCCTC TTTATTAGCTCCTAGCAGTGGCCTTTCAGTTCAAAACTTCCCACCAGGGCTTTACTGCAAAACAAGCATG GGGCAGCAAAAGCCAACTGCATACGTCAGACCCATGGATGGCCAGGACCAGGCACCGGACATCTCACCAA CACTGAAACCTTCAATTGAATTTGAGAACAGCTTTGGGAATCTGTCATTTGGAACACTCTTGGATGGAAA ACCCAGTGCAGCCAGTTCAAAGACTAAACTGCCAAAGTTCACCATCCTCCAAACAAGTGAAGTAAGCCTT CCCAGTGATCCAAGCTGTGTTGAAGAAATCTTGCGGGAGATGACCCATTCCTGGCCTACTCCTCTCACTT CCATGCATACTGCTGGACACTCTGAGCAGAGCACCTTTTCCATCCCAGGACAGGAATCGCAGCATCTGAC CCCAGGATTCACCTTACAAAAGTGGAATGACCCAACCACCAGAGCTTCTACAAAGTCAGTGTCTTTCAAA TCGATGCTTGAGGATGACCTGAAGCTGAGCAGTGATGAAGATGACCTTGAGCCTGTGAAGACCTTGACCA CTCAGTGCACTGCCACTGAGCTCTACCAGGCTGTTGAAAAGGCAAAACCTAGGAATAATCCTGTGAACCC ACCCTTGGCCACTCCCCAGCCCCCACCTGCAGTGCAAGCCAGCGGGGGTTCTGGCAGCTCCAGCGAATCG GAGAGCAGCTCTGAGTCGGATTCAGACACTGAAAGTAGCACCACTGACAGCGAATCTAATGAGGCACCTC GTGTGGCAACTCCAGAGCCTGAGCCACCCTCAACCAACAAGTGGCAACTGGATAAATGGCTTAACAAAGT GACATCCCAGAACAAGTCTTTTATTTGTGGCCCAAATGAAACACCCATGGAGACTATTTCTCTGCCTCCT CCAATCATCCAACCAATGGAAGTCCAGATGAAAGTGAAGACGAATGCCAGTCAGGTCCCAGCTGAACCCA AAGAAAGGCCTCTCCTCAGTCTCATTAGGGAGAAAGCCCGTCCACGGCCCACTCAGAAAATTCCAGAAAC AAAGGCTTTGAAGCATAAGTTGTCAACAACTAGTGAGACAGTGTCTCAAAGGACAATTGGGAAAAAACAG CCCAAAAAAGTTGAGAAGAACACCAGCACTGACGAGTTTACCTGGCCCAAACCAAATATTACCAGCAGCA CTCCCAAAGAAAAAGAAAGTGTGGAGCTTCATGACCCACCAAGAGGCCGCAACAAAGCCACTGCCCACAA ACCAGCCCCTAGGAAAGAACCAAGACCTAACATCCCTTTGGCTCCCGAGAAGAAGAAGTACAGAGGGCCT GGCAAGATTGTGCCAAAGTCTCGGGAATTCATTGAAACAGATTCATCTACATCTGACTCCAACACAGATC AGGAAGAGACCCTGCAAATCAAAGTCCTGCCTCCGTGCATTATTTCTGGAGGTAATACTGCCAAATCCAA GGAAATCTGTGGTGCCAGCCTGACCCTCAGCACCTTAATGAGTAGCAGTGGCAGCAACAACAACTTATCC ATCAGTAATGAAGAGCCAACATTTTCACCTATTCCTGTCATGCAAACTGAAATCCTGTCCCCTCTGCGAG ATCATGAGAACCTGAAAAACCTCTGGGTGAAGATTGACCTTGACTTACTCTCTAGAGTACCTGGCCACAG CTCACTCCATGCAGCACCTGCCAAGCCAGACCACAAGGAGACTGCCACAAAACCCAAGCGTCAGACAGCT GTCACAGCTGTGGAGAAACCAGCCCCTAAGGGCAAACGTAAGCACAAGCCAATAGAAGTTGCAGAGAAGA TCCCTGAGAAGAAGCAGCGCCTGGAGGAGGCCACAACTATCTGCTTGCTCCCTCCTTGCATCTCACCAGC CCCACCCCACAAGCCTCCCAACACTAGAGAAAATAATTCATCCAGGAGAGCAAATAGAAGAAAGGAAGAA AAACTATTTCCTCCTCCACTTTCCCCACTGCCAGAGGACCCTCCACGCCGCAGAAATGTCAGTGGCAATA ATGGTCCCTTTGGTCAAGACAAAAACATCGCCATGACTGGACAAATCACATCTACCAAACCTAAGAGAAC TGAAGGCAAATTCTGTGCTACTTTCAAAGGGATATCGGTAAATGAGGGAGACACTCCAAAAAAGGCATCC TCTGCCACCATCACTGTCACCAATACTGCTATTGCCACTGCTACTGTCACTGCTACTGCCATTGTCACCA CCACTGTCACAGCTACTGCCACCGCCACGGCCACCACCACAACTACTACCACTACCATTTCCACCATCAC CTCTACCATCACTACTGGCCTCATGGATAGCAGTCACCTGGAGATGACGTCCTGGGCGGCTCTGCCCCTT CTATCCAGCAGCAGCACTAATGTCCGGAGACCCAAGCTCACTTTTGATGACTCGGTTCACAATGCTGATT ATTACATGCAAGAAGCTAAGAAGCTGAAGCACAAAGCTGATGCACTGTTCGAGAAATTTGGCAAAGCTGT GAATTATGCTGATGCCGCCCTCTCCTTCACTGAATGTGGCAATGCCATGGAACGCGACCCTCTGGAAGCA AAGTCCCCATACACCATGTACTCTGAGACTGTGGAGCTCCTCAGGTATGCAATGAGGCTGAAGAACTTTG CAAGTCCCTTGGCTTCGGATGGGGACAAAAAGCTAGCAGTACTATGCTACCGATGTTTATCACTCCTCTA TTTGAGAATGTTTAAGCTGAAGAAGGACCATGCTATGAAGTACTCCAGATCACTGATGGAATATTTTAAG CAAAATGCTTCAAAAGTCGCACAGATACCCTCTCCATGGGTAAGCAATGGAAAGAACACTCCATCCCCAG TGTCTCTCAACAACGTCTCCCCCATCAACGCAATGGGGAACTGTAACAATGGCCCAGTCACCATTCCCCA GCGCATTCACCACATGGCTGCCAGCCACGTCAACATCACTAGCAATGTGTTACGGGGCTATGAACACTGG GATATGGCCGACAAACTGACAAGAGAAAACAAAGAATTCTTTGGTGATCTGGACACGCTGATGGGGCCTC TGACCCAGCACAGCAGCATGACCAATCTTGTCCGCTACGTTCGCCAAGGACTGTGTTGGCTGCGCATCGA TGCCCACTTGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212045 representing NM_002025
Red=Cloning site Green=Tags(s) MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLFSSGFDLFGEPYKVAEYTNKGDALANR VQNTLGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPS VVILNSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVE ESNPSAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSM GQQKPTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLPKFTILQTSEVSL PSDPSCVEEILREMTHSWPTPLTSMHTAGHSEQSTFSIPGQESQHLTPGFTLQKWNDPTTRASTKSVSFK SMLEDDLKLSSDEDDLEPVKTLTTQCTATELYQAVEKAKPRNNPVNPPLATPQPPPAVQASGGSGSSSES ESSSESDSDTESSTTDSESNEAPRVATPEPEPPSTNKWQLDKWLNKVTSQNKSFICGPNETPMETISLPP PIIQPMEVQMKVKTNASQVPAEPKERPLLSLIREKARPRPTQKIPETKALKHKLSTTSETVSQRTIGKKQ PKKVEKNTSTDEFTWPKPNITSSTPKEKESVELHDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGP GKIVPKSREFIETDSSTSDSNTDQEETLQIKVLPPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLS ISNEEPTFSPIPVMQTEILSPLRDHENLKNLWVKIDLDLLSRVPGHSSLHAAPAKPDHKETATKPKRQTA VTAVEKPAPKGKRKHKPIEVAEKIPEKKQRLEEATTICLLPPCISPAPPHKPPNTRENNSSRRANRRKEE KLFPPPLSPLPEDPPRRRNVSGNNGPFGQDKNIAMTGQITSTKPKRTEGKFCATFKGISVNEGDTPKKAS SATITVTNTAIATATVTATAIVTTTVTATATATATTTTTTTTISTITSTITTGLMDSSHLEMTSWAALPL LSSSSTNVRRPKLTFDDSVHNADYYMQEAKKLKHKADALFEKFGKAVNYADAALSFTECGNAMERDPLEA KSPYTMYSETVELLRYAMRLKNFASPLASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFK QNASKVAQIPSPWVSNGKNTPSPVSLNNVSPINAMGNCNNGPVTIPQRIHHMAASHVNITSNVLRGYEHW DMADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVRYVRQGLCWLRIDAHLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002025 |
| ORF Size | 3933 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002025.1, NP_002016.1 |
| RefSeq Size | 13695 bp |
| RefSeq ORF | 3936 bp |
| Locus ID | 2334 |
| UniProt ID | P51816 |
| Domains | AF-4 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212045 | AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 1 |
CNY 9,128.00 |
|
| RC212045L3 | Lenti-ORF clone of AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 1 |
CNY 15,680.00 |
|
| RC212045L4 | Lenti-ORF clone of AFF2 (mGFP-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 1 |
CNY 15,680.00 |
|
| SC125530 | AFF2 (untagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 1 |
CNY 9,136.00 |
