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NODAL (NM_018055) Human Tagged ORF Clone
CAT＃: RG211302
- TrueORF®
NODAL (tGFP-tagged) - Human nodal homolog (mouse) (NODAL)
ORF Plasmid: DDK
"NM_018055" in other vectors (6)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HTX5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211302 representing NM_018055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACGCCCACTGCCTGCCCTTCCTTCTGCACGCCTGGTGGGCCCTACTCCAGGCGGGTGCTGCGACGG TGGCCACTGCGCTCCTGCGTACGCGGGGGCAGCCCTCGTCGCCATCCCCTCTGGCGTACATGCTGAGCCT CTACCGCGACCCGCTGCCGAGGGCAGACATCATCCGCAGCCTACAGGCAGAAGATGTGGCAGTGGATGGG CAGAACTGGACGTTTGCTTTTGACTTCTCCTTCCTGAGCCAACAAGAGGATCTGGCATGGGCTGAGCTCC GGCTGCAGCTGTCCAGCCCTGTGGACCTCCCCACTGAGGGCTCACTTGCCATTGAGATTTTCCACCAGCC AAAGCCCGACACAGAGCAGGCTTCAGACAGCTGCTTAGAGCGGTTTCAGATGGACCTATTCACTGTCACT TTGTCCCAGGTCACCTTTTCCTTGGGCAGCATGGTTTTGGAGGTGACCAGGCCTCTCTCCAAGTGGCTGA AGCGCCCTGGGGCCCTGGAGAAGCAGATGTCCAGGGTAGCTGGAGAGTGCTGGCCGCGGCCCCCCACACC GCCTGCCACCAATGTGCTCCTTATGCTCTACTCCAACCTCTCGCAGGAGCAGAGGCAGCTGGGTGGGTCC ACCTTGCTGTGGGAAGCCGAGAGCTCCTGGCGGGCCCAGGAGGGACAGCTGTCCTGGGAGTGGGGCAAGA GGCACCGTCGACATCACTTGCCAGACAGAAGTCAACTGTGTCGGAAGGTCAAGTTCCAGGTGGACTTCAA CCTGATCGGATGGGGCTCCTGGATCATCTACCCCAAGCAGTACAACGCCTATCGCTGTGAGGGCGAGTGT CCTAATCCTGTTGGGGAGGAGTTTCATCCGACCAACCATGCATACATCCAGAGTCTGCTGAAACGTTACC AGCCCCACCGAGTCCCTTCCACTTGTTGTGCCCCAGTGAAGACCAAGCCGCTGAGCATGCTGTATGTGGA TAATGGCAGAGTGCTCCTAGATCACCATAAAGACATGATCGTGGAAGAATGTGGGTGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211302 representing NM_018055
Red=Cloning site Green=Tags(s) MHAHCLPFLLHAWWALLQAGAATVATALLRTRGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAEDVAVDG QNWTFAFDFSFLSQQEDLAWAELRLQLSSPVDLPTEGSLAIEIFHQPKPDTEQASDSCLERFQMDLFTVT LSQVTFSLGSMVLEVTRPLSKWLKRPGALEKQMSRVAGECWPRPPTPPATNVLLMLYSNLSQEQRQLGGS TLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGEC PNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018055 |
| ORF Size | 1041 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018055.3, NP_060525.2 |
| RefSeq Size | 1744 bp |
| RefSeq ORF | 1044 bp |
| Locus ID | 4838 |
| UniProt ID | Q96S42 |
| Protein Families | Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway |
| Protein Pathways | TGF-beta signaling pathway |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211302 | NODAL (Myc-DDK-tagged)-Human nodal homolog (mouse) (NODAL) |
CNY 5,488.00 |
|
| RC211302L1 | Lenti ORF clone of Human nodal homolog (mouse) (NODAL), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC211302L2 | Lenti ORF clone of Human nodal homolog (mouse) (NODAL), mGFP tagged |
CNY 5,890.00 |
|
| RC211302L3 | Lenti ORF clone of Human nodal homolog (mouse) (NODAL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211302L4 | Lenti ORF clone of Human nodal homolog (mouse) (NODAL), mGFP tagged |
CNY 5,890.00 |
|
| SC304557 | NODAL (untagged)-Human nodal homolog (mouse) (NODAL) |
CNY 5,488.00 |
