NAPE PLD (NAPEPLD) (NM_198990) Human Tagged ORF Clone
CAT#: RG209877
- TrueORF®
NAPEPLD (tGFP-tagged) - Human N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2
ORF Plasmid: DDK
"NM_198990" in other vectors (4)
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CNY 5,256.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C7orf18; FMP30; NAPE-PLD |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209877 representing NM_198990
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAAAATGAAAGCAACCAGTCTCTGATGACAAGCAGCCAATATCCTAAAGAAGCAGTAAGAAAAC GTCAAAATTCAGCACGGAATTCCGGAGCAAGTGATTCTTCTAGGTTTTCTAGGAAAAGCTTCAAACTGGA TTATAGACTAGAAGAAGATGTAACTAAATCCAAGAAAGGAAAAGATGGGAGATTTGTGAATCCGTGGCCA ACATGGAAAAACCCCTCTATTCCAAATGTTCTCAGATGGCTGATAATGGAGAAAGATCACAGCAGTGTTC CAAGTTCTAAAGAGGAACTAGACAAAGAACTCCCAGTGCTTAAGCCATATTTTATCACTAACCCTGAAGA AGCTGGAGTGAGGGAAGCTGGCTTAAGAGTCACATGGCTGGGACATGCCACGGTAATGGTGGAAATGGAT GAGCTCATATTTCTCACGGATCCCATCTTTAGCTCTCGTGCTTCACCATCGCAGTACATGGGTCCAAAGC GATTTCGTCGTTCCCCGTGCACAATAAGTGAACTCCCTCCAATAGATGCGGTCCTTATCAGTCACAACCA CTATGACCATCTGGACTACAATTCTGTCATTGCTTTGAATGAGCGATTTGGTAATGAGTTGAGATGGTTT GTGCCTTTGGGTCTCCTTGACTGGATGCAAAAATGTGGCTGTGAGAATGTGATTGAGTTGGACTGGTGGG AGGAGAATTGTGTCCCCGGACATGATAAGGTCACTTTTGTCTTTACACCTTCCCAGCACTGGTGTAAAAG GACTCTAATGGATGACAACAAGGTGCTATGGGGCAGCTGGTCTGTCTTGGGGCCTTGGAATCGATTTTTT TTCGCAGGAGATACTGGTTATTGCCCTGCTTTTGAAGAGATAGGAAAAAGATTTGGACCTTTTGACCTTG CAGCTATTCCCATCGGAGCTTATGAACCGAGGTGGTTTATGAAATACCAGCATGTAGACCCAGAAGAAGC TGTAAGGATTCACACTGATGTCCAAACAAAGAAATCTATGGCAATTCACTGGGGAACTTTTGCCTTAGCA AATGAGCATTACTTAGAGCCTCCAGTGAAGCTGAATGAAGCTCTAGAGAGATACGGACTTAACGCTGAAG ATTTTTTTGTCTTGAAGCATGGAGAATCAAGATACCTAAATAATAATGATGAAAACTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209877 representing NM_198990
Red=Cloning site Green=Tags(s) MDENESNQSLMTSSQYPKEAVRKRQNSARNSGASDSSRFSRKSFKLDYRLEEDVTKSKKGKDGRFVNPWP TWKNPSIPNVLRWLIMEKDHSSVPSSKEELDKELPVLKPYFITNPEEAGVREAGLRVTWLGHATVMVEMD ELIFLTDPIFSSRASPSQYMGPKRFRRSPCTISELPPIDAVLISHNHYDHLDYNSVIALNERFGNELRWF VPLGLLDWMQKCGCENVIELDWWEENCVPGHDKVTFVFTPSQHWCKRTLMDDNKVLWGSWSVLGPWNRFF FAGDTGYCPAFEEIGKRFGPFDLAAIPIGAYEPRWFMKYQHVDPEEAVRIHTDVQTKKSMAIHWGTFALA NEHYLEPPVKLNEALERYGLNAEDFFVLKHGESRYLNNNDENF TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_198990 |
ORF Size | 1179 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_198990.3, NP_945341.2 |
RefSeq Size | 4841 bp |
RefSeq ORF | 1182 bp |
Locus ID | 222236 |
UniProt ID | Q6IQ20 |
Gene Summary | NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM, Oct 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209877 | NAPEPLD (Myc-DDK-tagged)-Human N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2 |
CNY 3,656.00 |
|
RC209877L3 | Lenti ORF clone of Human N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209877L4 | Lenti ORF clone of Human N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC101779 | NAPEPLD (untagged)-Human N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2 |
CNY 3,656.00 |
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