GM130 (GOLGA2) (NM_004486) Human Tagged ORF Clone
CAT#: RG209641
- TrueORF®
GOLGA2 (tGFP-tagged) - Human golgin A2 (GOLGA2)
ORF Plasmid: DDK
"NM_004486" in other vectors (7)
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CNY 12,064.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GM130 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209641 representing NM_004486
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAAGAAACCCGACAGAGCAAATTGGCCGCAGCGAAGAAAAAGTTGAGAGAATATCAGCAGAGGA ATAGCCCTGGTGTTCCTACAGGAGCGAAAAAGAAGAAGAAAATAAAAAATGGCAGTAACCCTGAGACAAC CACTTCTGGTGGTTGCCACTCACCTGAGGATACACCCAAGGACAATGCTGCTACTCTACAACCATCTGAT GACACCGTGTTACCTGGCGGTGTCCCTTCCCCTGGTGCCAGTCTCACTAGCATGGCGGCATCTCAGAATC ATGATGCTGACAATGTCCCTAATCTCATGGATGAAACCAAGACTTTCTCATCAACCGAGAGCCTGCGACA ACTCTCCCAACAGCTCAATGGTCTTGTTTGTGAGTCTGCGACATGTGTCAATGGGGAGGGCCCTGCATCG TCTGCTAACCTGAAGGATCTGGAGAGCCGGTACCAACAGCTAGCGGTAGCCCTGGACTCCAGCTATGTAA CAAACAAACAACTCAATATCACGATAGAGAAATTGAAACAACAGAACCAAGAAATTACGGATCAGTTGGA AGAAGAAAAGAAAGAATGCCACCAAAAGCAGGGAGCCCTAAGGGAGCAGTTACAGGTTCACATTCAGACC ATAGGGATCCTCGTATCAGAGAAAGCTGAGTTACAGACAGCCCTGGCTCACACTCAGCATGCTGCCAGGC AGAAAGAAGGAGAGTCTGAAGATCTGGCCAGCCGCCTGCAGTATTCCCGGCGGCGTGTGGGAGAGTTGGA GCGGGCTCTCTCTGCTGTCTCCACGCAGCAGAAGAAGGCAGACAGGTACAACAAGGAGTTAACCAAAGAG AGAGACGCCCTCAGGCTGGAGTTATACAAGAACACCCAAAGCAATGAGGACCTGAAGCAAGAGAAATCAG AATTGGAAGAGAAGCTTCGGGTCCTAGTGACTGAGAAGGCTGGCATGCAGCTTAACTTGGAAGAATTGCA AAAGAAGTTAGAGATGACGGAACTCCTGCTTCAACAGTTTTCAAGCCGGTGTGAAGCCCCTGATGCTAAC CAGCAGTTACAGCAGGCCATGGAGGAGCGGGCACAGCTGGAAGCACACCTGGGGCAGGTAATGGAGTCGG TTAGACAACTACAAATGGAGAGAGATAAATATGCGGAGAATCTCAAAGGAGAGAGCGCCATGTGGCGGCA GAGGATGCAGCAGATGTCAGAGCAGGTGCACACATTGAGAGAGGAGAAGGAATGTAGCATGAGTCGGGTA CAGGAGCTGGAGACGAGCTTGGCTGAACTGAGGAACCAGATGGCTGAACCCCCGCCCCCAGAGCCCCCAG CAGGGCCCTCCGAGGTGGAGCAGCAGCTACAAGCGGAGGCTGAGCACCTGCGGAAGGAGCTGGAGGGTCT GGCAGGACAGCTTCAAGCCCAGGTGCAAGACAATGAGGGCTTGAGTCGCCTGAACCGGGAGCAGGAGGAG AGGCTGCTGGAGCTGGAGCGGGCGGCCGAGCTCTGGGGGGAGCAGGCGGAGGCGCGCAGGCAAATCCTGG AGACCATGCAGAACGACCGCACTACCATCAGCCGCGCACTCTCCCAGAACCGGGAGCTCAAGGAGCAGCT GGCTGAGCTGCAGAGCGGATTTGTAAAGCTGACTAATGAGAACATGGAGATCACCAGCGCACTGCAGTCG GAGCAGCACGTCAAGAGGGAGCTGGGAAAGAAGCTGGGCGAGCTGCAGGAGAAGCTGAGCGAGCTGAAGG AAACGGTGGAGCTGAAGAGCCAAGAGGCTCAAAGTCTGCAGCAGCAGCGAGACCAGTACCTGGGACACCT GCAGCAGTATGTGGCCGCCTATCAGCAGCTGACCTCTGAGAAGGAGGTGCTGCATAATCAGCTACTGCTG CAGACCCAGCTCGTGGACCAGCTGCAGCAGCAGGAAGCTCAGGGCAAAGCGGTGGCCGAGATGGCCCGCC AAGAGTTGCAGGAAACCCAGGAGCGCCTGGAAGCTGCCACCCAGCAGAATCAGCAGCTACGGGCCCAGTT GAGCCTCATGGCTCACCCTGGGGAAGGAGATGGACTGGACCGGGAGGAGGAGGAGGATGAGGAGGAGGAG GAGGAGGAGGCGGTGGCAGTACCTCAGCCCATGCCAAGCATCCCGGAGGACCTGGAGAGCCGGGAAGCCA TGGTGGCATTTTTCAACTCAGCTGTAGCCAGTGCCGAGGAGGAGCAGGCAAGGCTACGTGGGCAGCTGAA GGAGCAAAGGGTGCGCTGCCGGCGCCTGGCTCACCTGCTGGCCTCGGCCCAGAAGGAGCCTGAGGCAGCA GCCCCAGCCCCAGGGACCGGGGGTGATTCTGTGTGTGGGGAGACCCACCGGGCCCTGCAGGGGGCCATGG AGAAGCTGCAGAGCCGCTTTATGGAGCTCATGCAGGAGAAGGCAGACCTGAAGGAGAGGGTAGAGGAACT GGAACATCGCTGCATCCAGCTTTCTGGAGAGACAGACACCATTGGAGAGTACATTGCACTGTACCAGAGC CAGAGGGCAGTGCTGAAGGAGCGGCACCGGGAGAAGGAGGAGTACATCAGCAGGCTGGCCCAAGACAAGG AGGAGATGAAGGTGAAGCTGCTGGAGCTGCAGGAGCTGGTCTTACGGCTTGTGGGCGACCGCAACGAGTG GCATGGCAGATTCCTGGCAGCTGCCCAGAACCCTGCTGATGAGCCCACTTCAGGGGCCCCAGCCCCCCAG GAACTTGGGGCTGCCAACCAGCAGGGTGATCTTTGCGAGGTGAGCCTCGCCGGCAGTGTGGAGCCTGCCC AAGGAGAGGCCAGGGAGGGTTCTCCCCGTGACAACCCCACTGCACAGCAGATCATGCAGCTGCTTCGTGA GATGCAGAACCCCCGGGAGCGCCCAGGCTTGGGCAGCAACCCCTGCATTCCTTTTTTTTACCGGGCTGAC GAGAATGATGAGGTGAAGATCACTGTCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209641 representing NM_004486
Red=Cloning site Green=Tags(s) MSEETRQSKLAAAKKKLREYQQRNSPGVPTGAKKKKKIKNGSNPETTTSGGCHSPEDTPKDNAATLQPSD DTVLPGGVPSPGASLTSMAASQNHDADNVPNLMDETKTFSSTESLRQLSQQLNGLVCESATCVNGEGPAS SANLKDLESRYQQLAVALDSSYVTNKQLNITIEKLKQQNQEITDQLEEEKKECHQKQGALREQLQVHIQT IGILVSEKAELQTALAHTQHAARQKEGESEDLASRLQYSRRRVGELERALSAVSTQQKKADRYNKELTKE RDALRLELYKNTQSNEDLKQEKSELEEKLRVLVTEKAGMQLNLEELQKKLEMTELLLQQFSSRCEAPDAN QQLQQAMEERAQLEAHLGQVMESVRQLQMERDKYAENLKGESAMWRQRMQQMSEQVHTLREEKECSMSRV QELETSLAELRNQMAEPPPPEPPAGPSEVEQQLQAEAEHLRKELEGLAGQLQAQVQDNEGLSRLNREQEE RLLELERAAELWGEQAEARRQILETMQNDRTTISRALSQNRELKEQLAELQSGFVKLTNENMEITSALQS EQHVKRELGKKLGELQEKLSELKETVELKSQEAQSLQQQRDQYLGHLQQYVAAYQQLTSEKEVLHNQLLL QTQLVDQLQQQEAQGKAVAEMARQELQETQERLEAATQQNQQLRAQLSLMAHPGEGDGLDREEEEDEEEE EEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAA APAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQS QRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQ ELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRAD ENDEVKITVI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004486 |
ORF Size | 2970 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004486.5 |
RefSeq Size | 4304 bp |
RefSeq ORF | 3009 bp |
Locus ID | 2801 |
UniProt ID | Q08379 |
Domains | M |
Gene Summary | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Regulation of the Golgi apparatus via GOLPH3-mediated new selective autophagy
,Lu, LQ;Tang, MZ;Qi, ZH;Huang, SF;He, YQ;Li, DK;Li, LF;Chen, LX;,
Life Sci.
,PubMed ID 32335164
[GOLGA2]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209641 | GOLGA2 (Myc-DDK-tagged)-Human golgin A2 (GOLGA2) |
CNY 10,464.00 |
|
RC209641L1 | Lenti-ORF clone of GOLGA2 (Myc-DDK-tagged)-Human golgin A2 (GOLGA2) |
CNY 12,864.00 |
|
RC209641L2 | Lenti-ORF clone of GOLGA2 (mGFP-tagged)-Human golgin A2 (GOLGA2) |
CNY 12,864.00 |
|
RC209641L3 | Lenti-ORF clone of GOLGA2 (Myc-DDK-tagged)-Human golgin A2 (GOLGA2) |
CNY 7,600.00 |
|
RC209641L4 | Lenti-ORF clone of GOLGA2 (mGFP-tagged)-Human golgin A2 (GOLGA2) |
CNY 7,600.00 |
|
SC310019 | GOLGA2 (untagged)-Human golgin A2 (GOLGA2) |
CNY 10,472.00 |
|
SC321016 | GOLGA2 (untagged)-Human golgin A2 (GOLGA2) |
CNY 10,472.00 |